Oncostatin M and TLR-4 Ligand Synergize to Induce MCP-1, IL-6, and VEGF in Human Aortic Adventitial Fibroblasts and Smooth Muscle Cells by David Schnittker, Karen Kwofie, Ali Ashkar, Bernardo Trigatti, Carl D. Richards

“…Signalling pathways including STATs, MAPKinases, and NFκB were activated, and LPS induced steady state mRNA levels of the OSM receptor chains OSMRβ and gp130. …”
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β-淀粉样蛋白对叉头蛋白O3a和突触后致密蛋白95的影响 by 牛波, 王欣怡, 徐江平, 汪海涛

“…RT-PCR结果显示Aβ_25-35组大鼠海马组织中PSD95的mRNA水平下降为对照组的（58.40±8.28） % （<italic>F</italic>=1.193， <italic>P</italic>=0.010 1），同时FoxO3a的mRNA的表达量上调（140.90±7.45）% （<italic>F</italic>=2.378， <italic>P</italic>=0.049 6）。…”
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Dietary Solid-state-fermentation product of Bacillus velezensis T23 alleviate hepatic steatosis, oxidative stress, gut barrier damage, and microbiota dysbiosis in juvenile genetica... by Qiang Hao, Rolf Erik Olsen, Einar Ringø, Qianwen Ding, Tsegay Teame, Yuanyuan Yao, Chao Ran, Yalin Yang, Zhen Zhang, Zhigang Zhou

“…After 4 weeks of the feeding trial, the results showed that T23 supplementation notably reduced the hepatocytes vacuolization and the content of liver TAGs by downregulated the mRNA expression of the lipogenesis gene (fas), while upregulated the mRNA expression of lipid degradation genes (cpt1 and pparα) (p < 0.05). …”
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Jiangu Granules Regulates Endoplasmic Reticulum Stress-Induced Apoptosis in UMR-106 Cells by Modulating the PERK/eIF2α/ATF4/CHOP Pathway by CHEN Sainan, ZHOU Fen, HUANG Yunmei, LIN Yanping

“…In comparison to the NC group, the apoptosis rate in the H₂O₂ group displayed a significant increase (<italic>P</italic>&lt;0.05). Furthermore, the mRNA transcription level and protein expression level of ATF4 and CHOP, as well as GRP78, PERK, and eIf2α, exhibited a significant increase (<italic>P</italic>&lt;0.01). …”
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Exploring Future Pandemic Preparedness Through the Development of Preventive Vaccine Platforms and the Key Roles of International Organizations in a Global Health Crisis by Jihee Jeon, Eunyoung Kim

“…The need for rapid vaccine development has been highlighted by Coronavirus disease 2019 (COVID-19), and mRNA technology has shown promise as a platform. …”
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Dynamic Profile of CD4+ T-Cell-Associated Cytokines/Chemokines following Murine Myocardial Infarction/Reperfusion by Dongsheng Yuan, Jinjun Tie, Zhican Xu, Guanya Liu, Xinyu Ge, Zhulin Wang, Xumin Zhang, Shiyu Gong, Gang Liu, Qingshu Meng, Fang Lin, Zhongmin Liu, Huimin Fan, Xiaohui Zhou

“…IL-13 and MIP-1β showed higher levels in the MI/R serum at day 14 than at day 7. mRNA levels of IL-4, IL-6, IL-13, and IL-27 were increased in the day 7 group compared to the sham, while MIP-1β, MCP-3, and GRO-α mRNA levels showed no significant difference between the MI/R and sham groups in blood mononuclear cells. …”
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Creation of body color mutants by CRISPR/Cas9 gene editing in largemouth bass, Micropterus salmoides by Lina Sun, Tian Gao, Zhiqiang Li, Xiaolong Yang, Zuliang Qin, Mingxiao Ye, Ying Li, Feilong Wang, Deshou Wang

“…The efficiency of mutation was gRNA and Cas9 mRNA concentration dependent, and 400 ng/μl gRNA and 800 ng/μl Cas9 mRNA were recommended as the appropriate injection dose for genome editing in LMB. …”
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N-acetylcysteine increased the growth performance of Leuciscus waleckii by improving mitochondrial function, enhancing antioxidant and anti-inflammatory, and inhibiting apoptosis i... by Deng Lai Li, Rui Zhu, Si Tong Meng, Yin Tao Wang, Si Ying Liu, Zhi Yong Yang, Li Fang Wu

“…Meanwhile, 4.50 g/kg-6.00 g/kg NAC reduced the mRNA expression of hepatopancreatic and intestinal Bax, Caspase-3 and Caspase-9, and Caspase-3, Caspase-8 and Caspase-9 enzyme activities, and inhibited apoptosis. …”
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Effect of Traditional Chinese Medicine on Inflammatory Mediators in Pediatric Asthma by Hui Du, Yonghong Wang, Yumin Shi, Jian Yu, Wen Sun, Yiqun Zhang

“…Real-time PCR was used to determine the mRNA expression levels of interleukin- (IL-) 10, IL-17, matrix metalloproteinase-9 (MMP-9), and transforming growth factor β1 (TGF-β1) in peripheral blood mononuclear cells before and after treatment. …”
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Combined Effects of Chrysin Supplementation and Exercise Training on Diabetes-Induced Oxidative Stress and Apoptosis in Rat Testicular Tissue by Shiva Cheraghi Abajlou, Asghar Tofighi, Javad Tolouei Azar, Amir Afshin Khaki, Mazdak Razi

“…Following 8 weeks, the histopathological changes (Johnson’s score, epithelial height,and tubular diameter), testicular malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase(GPX), and the mRNA levels of anti-apoptotic gene Bcl-2 and pro-apoptotic gene Bax was analyzed.Results: Chrysin solely and simultaneous with MIRET could remarkably (P=0.001) improve the DM-induced histopathologicaldamages, increase the testicular SOD and GPx levels, and decline the DM-increased MDA content.Moreover, our results showed that Chrysin solely and more simultaneously with MIRET could significantly (P=0.001)decrease the mRNA expression of Bax and improve the Bcl-2 expression and rebalance the Bax/Bcl-2 balance.Conclusion: Our findings showed that co-administration of Chrysin along with MIRET can significantly amelioratethe DM-induced histopathological, and biochemical impairments and reduce the pro-apoptotic impact of DMon testicular tissue.…”
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Polarization of ILC2s in Peripheral Blood Might Contribute to Immunosuppressive Microenvironment in Patients with Gastric Cancer by Qingli Bie, Pan Zhang, Zhaoliang Su, Dong Zheng, Xinyu Ying, Yumin Wu, Huijian Yang, Deyu Chen, Shengjun Wang, Huaxi Xu

“…The increased frequency of ILC2s was also found in PBMC of patients by flow cytometry. In addition, the mRNA of Arg1 and iNOS were also significantly increased in patients. …”
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Comprehensive Analysis of Differentially Expressed miRNAs and mRNAs Reveals That miR-181a-5p Plays a Key Role in Diabetic Dermal Fibroblasts by Peng Liu, Yi Zhu, Qin Li, Biao Cheng

“…We searched the GEO database for related miRNA (GSE68185, GSE84971) and mRNA (GSE49566, GSE78891) profiles. After eliminating batch effects and identifying differentially expressed genes (DEGs), we applied enrichment analyses and found that 3 miRNAs and 30 mRNAs were differentially expressed in diabetic HDFs. …”
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Dose-dependent role of AMH and AMHR2 signaling in male differentiation and regulation of sex determination in Spotted knifejaw (Oplegnathus punctatus) with X1X1X2X2/X1X2Y chromosom... by Haixia Zhao, Jun Li, Zhizhong Xiao, Yongshuang Xiao

“…Notably, the specific mRNA expression of amhr2 is significantly enriched in amhr2cy, whose extracellular domain exhibits the highest binding affinity for Amh protein, with sexual expression differences manifesting as early as 5 dph. …”
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Serum Glial Cell Line-Derived Neurotrophic Factor (sGDNF) Is a Novel Biomarker in Predicting Cirrhosis in Patients with Chronic Hepatitis B by Guangyue Yang, Liping Zhuang, Tiantian Sun, Yee Hui Yeo, Le Tao, Wei Zhang, Wenting Ma, Liu Wu, Zongguo Yang, Yanqin Yang, Dongying Xue, Jie Zhang, Rilu Feng, Ebert Matthias P., Steven Dooley, Ekihiro Seki, Ping Liu, Cheng Liu

“…Conclusion. Using serum, tissue mRNA, and biopsy data, our study revealed a significant potential of sGDNF as a novel noninvasive biomarker for cirrhosis in CHB patients.…”
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抵抗素对3T3-L1脂肪细胞葡萄糖摄取的影响 by 李芳萍, 李焱, 傅玉如, 严励, 傅祖植

“…【结果】插入pcDNA3．1^（＋）的DNA片段的核苷酸序列与小鼠抵抗素基因mRNA编码区序列完全一致．且方向正确：插入pcDNA3．10^（＋）的DNA片段的核苷酸序列与抵抗素基因mRNA编码区核苷酸序列完全一致，且方向相反。…”
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ｍｉＲ－１０３通过抑制ＦＢＷ７的表达促进肝癌细胞增殖

“…Ｒｅａｌｔｉｍｅ ＰＣＲ和Ｗｅｓｔｅｒｎ ｂｌｏｔｔｉｎｇ检测转染ｍｉＲ－１０３ ｍｉｍｉｃｓ后肝癌细胞中ＦＢＷ７ ｍＲＮＡ和蛋白的表达变化。通过重荧光素酶报告基因实验检测转染ｍｉＲ－１０３ｍｉｍｉｃｓ 或ｉｎｈｉｂｉｔｏｒ后ＦＢＷ７－３’ＵＴＲ活性的变化。…”
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Spontaneous tumor regression following COVID-19 vaccination by Hui Dai, Renata Ferrarotto, Myrna Godoy, Diana Bell, Shiaw-Yih Lin, Daniel J McGrail, Luana Guimaraes de Sousa, Kaiyi Li, Mario L Marques-Piubelli, Cipriano Gonzalez, Sammy Ferri-Borgogno, Jared Burks

“…Insight: This case suggests that the mRNA-1273 COVID-19 vaccine stimulated anticancer immunity and tumor regression.…”
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Triiodothyronine (T3) increases the expression of the amphiregulin (AREG) oncogene by activating extranuclear pathways in MCF-7 breast cancer cells by Maria Teresa De Sibio, Fernanda Cristina Fontes Moretto, Regiane Marques Castro Olimpio, Miriane de Oliveira, Lucas Solla Mathias, Vinícius Vigliazzi Peghinelli, Helena Paim Tilli, Bianca Mariani Gonçalves, Dariane Beatriz Marino Cardoso, Larissa Silva Dall Aqua, Igor de Carvalho Depra, Mariana Menezes Lourenço, Aline Carbonera Luvizon, Paula de Oliveira Montandon Hokama, Maria Tereza Nunes, Marna Eliana Sakalem, Célia Regina Nogueira

“…A control group (C) received no T3 or inhibitors. Analyses of mRNA and protein expression were done using RT-qPCR and Western blot, respectively. …”
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Dietary γ-Aminobutyric Acid Promotes Growth and Immune System Performance and Improves Erythropoiesis and Angiogenesis in Gibel Carp (<i>Carassius auratus gibelio</i>) by Xinlan Bai, Lu Zhang, Hualiang Liang, Dongyu Huang, Mingchun Ren, Haifeng Mi

“…In terms of oxygen-carrying capacity, the mRNA expression of <i>epo</i>, <i>tf</i>, <i>tfr1</i>, <i>ho-1</i>, and <i>vegf</i> was markedly increased at the level of 449 mg/kg (G2). …”
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TSP-1-1223 A/G Polymorphism as a Potential Predictor of the Recurrence Risk of Bladder Cancer in a Chinese Population by Xiao Yang, Pengchao Li, Xuejian Yang, Chao Qin, Qiang Cao, Zhengdong Zhang, Meilin Wang, Hongzhou Cai, Jinbao Gu, Jun Tao, Min Gu, Qiang Lu, Changjun Yin

“…The expression of TSP-1 mRNA in bladder cancer tissues was lower in patients with an AG genotype than in those with an AA genotype. …”
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