Transcriptome analysis of muscle atrophy in Leizhou black goats: identification of key genes and insights into limb-girdle muscular dystrophy by Ke Wang, Mengning Xu, Xiaotao Han, Hu Liu, Jiancheng Han, Wei Sun, Hanlin Zhou

“…Results Differential expression analysis revealed 536 mRNAs with significant differences across three muscle groups. Marked variations in mRNA expression patterns were observed between leg and back muscles versus abdominal muscles, reflecting characteristics similar to those found in limb-girdle muscular dystrophy. …”
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The inhibition of ORMDL3 prevents Alzheimer's disease through ferroptosis by PERK/ATF4/HSPA5 pathway by Yankun Shao, Yilin Xu, Huang Di, Xinxiu Shi, Yingying Wang, Hongyu Liu, Lina Song

“…In serum samples of AD patients, ORMDL3 mRNA expression levels were upregulated. The serum ORMDL3 levels expression was positively related to the ADL score or MoCA score in AD patients. …”
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Acylated Ghrelin Renders Chemosensitive Ovarian Cancer Cells Resistant to Cisplatin Chemotherapy via Activation of the PI3K/Akt/mTOR Survival Pathway by Attalla Farag El-kott, Ali A. Shati, Mohammed Ali Al-kahtani, Sultan Alqahtani

“…AG treatment did not affect the mRNA and protein levels of GHS-R1a and GHS-R1b in both control and Cis-treated cells. …”
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Notch Signaling Pathway Is Involved in bFGF-Induced Corneal Lymphangiogenesis and Hemangiogenesis by Fang Xie, Xue Zhang, Wenting Luo, Hongyan Ge, Dawei Sun, Ping Liu

“…Vascular endothelial growth factor-A (VEGF-A), Delta-like ligand 4 (Dll4), and Notch1 mRNA and protein expression were examined on days 1, 3, 7, and 14 by real-time polymerase chain reaction and western blot. …”
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Cytokine Detection and Modulation in Acute Graft vs. Host Disease in Mice by A. C. Knulst, G. J. M. Tibbe, C. Bril-Bazuin, E. G. Breedland, A. van Oudenaren, R. Benner, H. F. J. Savelkoul

“…In the allogeneic reconstituted mice the mRNA expression of IL-1α, IL-2, IL-6, IFN-γ and TNF-α displayed faster kinetics compared with that in syngeneic reconstituted mice. …”
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Effect of decitabine on PD-L2 methylation in whole blood of iodine-induced autoimmune thyroiditis rats by Baiming Jin, Yanbo Qi, Hong Chao, Xiaolei Yang, Hongjie Li, Siyuan Wan

“…The PD-L2 methylation levels were significantly decreased in EAT rats compared to control rats, and the mRNA expression of the PD-L2 was significantly increased. …”
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Differential Regulation of NK Cell Receptors in Acute Lymphoblastic Leukemia by Le Jie Lee, Norfarazieda Hassan, Siti Zuleha Idris, Suresh Kumar Subbiah, Heng Fong Seow, Norhafizah Mohtaruddin, Kian Meng Chang, Raudhawati Osman, Hishamshah Mohd Ibrahim, Sheila Nathan, Maha Abdullah

“…Quantitative PCR (q-PCR) further investigated copy number variation (CNV), methylation index (MI), and mRNA expression of significant genes in T-ALL (14 males), NC (12 males and 12 females), and B-ALL samples (N=12 males and 12 females). …”
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Prevention of Cyclophosphamide-Induced Immunosuppression in Mice with the Antimicrobial Peptide Sublancin by Shuai Wang, Shuo Huang, Qianhong Ye, Xiangfang Zeng, Haitao Yu, Desheng Qi, Shiyan Qiao

“…Furthermore, sublancin restored the mRNA levels of IL-2, IL-4, and IL-6 in the spleen. …”
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Radiotherapy Suppresses Bone Cancer Pain through Inhibiting Activation of cAMP Signaling in Rat Dorsal Root Ganglion and Spinal Cord by Guiqin Zhu, Yanbin Dong, Xueming He, Ping Zhao, Aixing Yang, Rubing Zhou, Jianhua Ma, Zhong Xie, Xue-Jun Song

“…Thermal hyperalgesia and mechanical allodynia were measured and used for evaluating level of pain caused by TCI treatment. PKA mRNA expression in dorsal root ganglion (DRG) was detected by RT-PCR. …”
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Ethnicity-specific distribution of <em>TRPM8</em> gene variants in Eurasian populations: signs of selection by T. A. Potapova, A. G. Romashchenko, N. S. Yudin, M. I. Voevoda

“…We analyzed sequences of five TRPM8 mRNA isoforms extracted by researchers from different tissues. …”
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rAAV2/1-SR-BI-IRES-EGFP转染HepG2肝细胞对 125I-HDL摄取的调节

“…【方法】 用含SR-BI基因的重组腺相关病毒rAAV2/1-SR-BI-IRES-EGFP杂合载体转染人HepG2肝细胞株,Realtime RT- PCR和Western Blot分别检测转染后SR-BI mRNA和蛋白的表达?125I-HDL同位素标记观察转染对HDL结合的影响?激光共聚焦显微镜观察转染后SR-BI细胞分布及转移情况?…”
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IGF2BP2 Regulates the Progression of Alzheimer's Disease Through m6A‐Mediated NLRP3 Inflammasome by Wu Jingrui, Yang Haihui, Yan Jinjin, Fang Le

“…Materials & Methods The mRNA and protein expression level were determined using RT‐qPCR and western blot, respectively. …”
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Effects of Antipsychotic Drugs on the Epigenetic Modification of Brain-Derived Neurotrophic Factor Gene Expression in the Hippocampi of Chronic Restraint Stress Rats by Mi Kyoung Seo, Young Hoon Kim, Roger S. McIntyre, Rodrigo B. Mansur, Yena Lee, Nicole E. Carmona, Ah Jeong Choi, Gyung-Mee Kim, Jung Goo Lee, Sung Woo Park

“…Chronic restraint stress resulted in the downregulation of total and exon IV BDNF mRNA levels and a decrease in histone H3 acetylation and an increase in MeCP2 binding at BDNF promoter IV. …”
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The Transcription Factor SOX18 Inhibitor Small Molecule 4 Is a Potential Treatment of Cancer‐Induced Lymphatic Metastasis and Lymphangiosarcoma by Katja K. Koll, Martin M. Zimmermann, Patrick A. Will, Ulrich Kneser, Christoph Hirche

“…We found that Sox18 exhibited high mRNA expression levels in both HDLEC and MO‐LAS. …”
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miR-126 Mimic Counteracts the Increased Secretion of VEGF-A Induced by High Glucose in ARPE-19 Cells by Roberta Sanguineti, Alessandra Puddu, Massimo Nicolò, Carlo Enrico Traverso, Renzo Cordera, Giorgio L. Viviani, Davide Maggi

“…After 24 hours, medium was replaced and cells were cultured for 24 hours in normal (CTR) or diabetic condition (HG). Then, we analyzed mRNA levels of miR-126, VEGF-A, PI3KR2, and SPRED1. …”
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AICAR Protects against High Palmitate/High Insulin-Induced Intramyocellular Lipid Accumulation and Insulin Resistance in HL-1 Cardiac Cells by Inducing PPAR-Target Gene Expression by Ricardo Rodríguez-Calvo, Manuel Vázquez-Carrera, Luis Masana, Dietbert Neumann

“…Then, cells were exposed to AICAR stimulation for 24 h in the presence or absence of the AMPK inhibitor Compound C, and the mRNA levels of the three PPARs were analyzed by real-time RT-PCR. …”
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SIRT6通过调节eNOS抑制心肌肥大的机制研究

“…【结果】 与PE刺激组相比,腺病毒过表达SIRT6能显著抑制PE诱导的心肌细胞ANF?BNP mRNA水平及心肌细胞表面积的增加(P < 0.05);与对照组相比,基因沉默SIRT6诱导心肌细胞ANF?…”
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Construction of cuproptosis-related genes risk model predicts the prognosis of Uterine Corpus Endometrial Carcinoma by Yanfang Huang, Guoxian Luo, Xiujie Sheng, Jianqi Li

“…Validation of the selected genes, CDKN2A, GLS, and PPAT, was performed at both mRNA and protein levels. A total of 27 cuproptosis-related genes were identified, with 19 upregulated and 6 downregulated in UCEC. …”
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Inhibiting METTLl3-ATG5 axis-mediated harmful autophagy in macrophages could help reduce airway epithelial inflammation and remodeling in COPD by Chen Guiyang, Xia Zhiwei, Zeng Xianyou, Liu Hanying

“…Methylated RNA immunoprecipitation-qPCR showed that METTL3 knockdown reduced m6A, and the actinomycin D assay suggested that METTL3 knockdown reduced ATG5 mRNA levels and lowered ATG5 mRNA stability. METTL3-knockdown RAW264.7 reduced the inflammation and airway remodeling markers in the co-cultured mouse bronchial epithelial cells. …”
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羟基红花黄色素A对脓毒症小鼠外周血促炎/抗炎因子的影响 by 王金平, 陈润桦, 赵佩研, 黄泽愉, 吴汉伟, 吴建龙

“…观察动物一般情况改变，检测血常规、血生化及血清白细胞介素-6（IL-6）、白细胞介素-10（IL-10）、肿瘤坏 死因子（TNF-α）的含量及mRNA表达水平，培养外周血细菌负荷量。【结果】术后24 h，模型组动物出现竖毛、肛门残留粪便 等；与对照组、假手术组、HSYA组相比，模型组小鼠全血白细胞计数（WBC）、血小板水平（PLT）明显下降，血清丙氨酸氨基 转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、尿素氮（BUN）、IL-6、IL-10、TNF-α含量及mRNA表达明显升高，细菌负荷量 明显增多，差异有统计学意义（P < 0.05）。…”
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