Pathogenic Role of Iron Deposition in Reticuloendothelial Cells during the Development of Chronic Hepatitis C by Hironori Mitsuyoshi, Kohichiroh Yasui, Kanji Yamaguchi, Masahito Minami, Takeshi Okanoue, Yoshito Itoh

“…The levels of serum soluble TNF-α receptor (sTNFR2) and hepatic hepcidin mRNA and the efficacy of phlebotomy were compared among patients with different iron deposition patterns. …”
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Bioactive milk-derived nutrient MFG-E8 ameliorates skeletal muscle atrophy induced by mitochondria damage in aging rats via activating the MAPK/ERK signaling pathway by Kaifang Guan, Xiaolin Liu, Weihong Lu, Yuhao Mao, Yirong Mao, Ying Ma, Rongchu Wang, Qiming Li

“…Flow cytometry and quantitative real-time PCR proved that MFG-E8 promoted cell proliferation by upregulating mRNA expression of cyclin D1, cyclin E1, CDK, and downregulating mRNA expression of p21 and p27, thereby increasing the S and G2/M phase and decreasing the G0/G1 phase. …”
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Impaired Glucose Metabolism in Young Offspring of Female Rats with Hypothyroidism by Zhoujun Liu, Yu Chen, Guofang Chen, Xiaodong Mao, Xiao Wei, Xingjia Li, Yijiao Xu, Fan Jiang, Kun Wang, Chao Liu

“…From birth to 8 weeks, pancreatic TRβ1 and TRβ2 mRNA level declined significantly in MH offspring, accompanied by decreased Ki67 and insulin mRNA expression. …”
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The Anti-Inflammatory Effects of Shinbaro3 Is Mediated by Downregulation of the TLR4 Signalling Pathway in LPS-Stimulated RAW 264.7 Macrophages by Hwa-Jin Chung, Wonil Koh, Won Kyung Kim, Joon-Shik Shin, Jinho Lee, Sang Kook Lee, In-Hyuk Ha

“…Shinbaro3 also downregulated the mRNA and protein expression of inflammatory mediators in a dose-dependent manner. …”
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链脲佐菌素诱导的１型糖尿病小鼠肾脏ＣＯＸ－２／ＰＧＥ２／ＥＰｓ的表达

“…ＥＬＩＳＡ法检测血清和尿液中前列腺素Ｅ２（ＰＧＥ２）的表达水平；ＲＴ－ｑＰＣＲ法检测肾组织环氧合酶－２（ＣＯＸ－２）、膜结合型前列腺素Ｅ２合成酶－１（ｍＰＧＥＳ－１）、各型ＥＰ受体（ＥＰ１、ＥＰ２、ＥＰ３、ＥＰ４）的ｍＲＮＡ表达水平；Ｗｅｓｔｅｒｎｂｌｏｔ 法检测相应蛋白表达水平。【结果】 随着周龄增加，ＤＭ组小鼠逐渐出现明显多饮、多尿、体质量下降等糖尿病消耗症状；与ＮＣ组小鼠相比，ＤＭ组小鼠空腹血糖（ＦＢＧ）和２４ ｈ尿蛋白排泄量均显著增加（Ｐ＜０．０５）；肾脏ＣＯＸ－２和ＥＰ４表达水平明显上调（Ｐ ＜ ０．０５）、而ｍＰＧＥＳ－１、ＥＰ１、ＥＰ２、ＥＰ３ ｍＲＮＡ表达无显著变化（Ｐ ＞ ０．０５）；同时，ＤＭ组小鼠２４ ｈ尿ＰＧＥ２定量显著高于ＮＣ组（Ｐ ＜ ０．０５）。…”
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miR-106a Is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B and Associated with Enhanced Levels of Interleukin-8 by Zhongsi Hong, Haiyu Hong, Jian Liu, Xiaobin Zheng, Mingxing Huang, Chunna Li, Jinyu Xia

“…Exogenous expression of miR-106a could significantly repress IL-8 expression at both mRNA and protein levels in PBMCs, whereas miR-106a inhibitor had the opposite effects. …”
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Tryptophan metabolism-related gene CYP1B1 serves as a shared biomarker for both Parkinson’s disease and insomnia by Xin-Yu Li, Wen-Kai Yu, Jing-Hao Wu, Wen-Jun He, Yu-Nan Cheng, Kai Gao, Yi-Han Wei, Yu-Sheng Li

“…Additionally, gene interaction, Drug-mRNA, Transcription Factor (TF)-mRNA, and competing endogenous RNA (ceRNA) networks centered on these hub genes were constructed to explore regulatory mechanisms and potential drug interactions. …”
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In silico identification of chilli genome encoded MicroRNAs targeting the 16S rRNA and secA genes of “Candidatus phytoplasma trifolii” by Vineeta Pandey, Aarshi Srivastava, Ramwant Gupta, Haitham E. M. Zaki, Haitham E. M. Zaki, Muhammad Shafiq Shahid, Rajarshi K. Gaur

“…The circos algorithm was then utilised to create the miRNA-mRNA regulatory network. The free energy between the miRNA:mRNA duplex was also computed, and the best value of −17.46 kcal/mol was obtained for CA-miR166c_2. …”
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Correlation of susceptibility to ortho-aminoazotolueneinduced hepatocarcinogenesis with Car and Ahr signaling pathway activation in mice by N. V. Baginskaya, E. V. Kashina, M. Yu. Shamanina, S. I. Ilnitskaya, V. I. Kaledin, V. A. Mordvinov

“…In this study, we investigated the effect of chronic OAT administration on the mRNA expression levels of Ahr, Car and their target genes Cyp1a1 and Cyp2b10 in the liver of DD/He (DD) and CC57BR/Mv (BR) mouse strains contrasting in sensitivity to hepatocarcinogenesis. …”
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Maternal obesity exacerbates the responsiveness of offspring BALB/c mice to cow’s milk protein-induced food allergy by Jingxin Gao, Tiange Li, Dong Liang, Han Gong, Liang Zhao, Xueying Mao

“…Furthermore, maternal obesity led to a lower level of occludin mRNA expression in BLG -sensitized mice. The mice born to obese mothers exhibited increased mRNA expression levels of GATA-3, interleukin (IL)-4 and IL-10 in jejunum after BLG sensitization, indicating maternal obesity intensified Th2-type biased immune responses. …”
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Light-regulated microRNAs shape dynamic gene expression in the zebrafish circadian clock. by Zuo Wang, Shuang Wang, Yi Bi, Alessandra Boiti, Shengxiang Zhang, Daniela Vallone, Xianyong Lan, Nicholas S Foulkes, Haiyu Zhao

“…However, considerable evidence points to post-transcriptional regulatory mechanisms, notably microRNAs (miRNAs), playing an essential role in shaping dynamic changes in mRNA levels. Therefore, does light directly impact the function of miRNAs? …”
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蛋白酶体抑制剂MG132 通过FOXO3a/Puma 通路对急性T 淋 巴细胞白血病细胞增殖凋亡的影响 by 张祖涵, 林文浩, 刘小俭, 梁聪, 罗学群, 黄礼彬

“…MG132作用于CCRF-CEM细胞后：FOXO3a的mRNA表达水平无明显改变，Puma的mRNA表 达明显上升；细胞中FOXO3a 及Puma 蛋白表达量增加；其中胞核中FOXO3a 蛋白表达量上升明显，胞浆中亦略有上升。 …”
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Neutrophils Are Dysregulated in Patients with Hereditary Angioedema Types I and II in a Symptom-Free Period by Tereza Grymova, Marcela Vlkova, Premysl Soucek, Roman Hakl, Jana Nechvatalova, Peter Slanina, Julie Stichova, Jiri Litzman, Tomas Freiberger

“…We assessed the relative mRNA expression of 10 genes related to neutrophil activation using RNA extracted from the peripheral blood neutrophils of 23 HAE patients in a symptom-free period and 39 healthy donors. …”
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Taenia crassiceps-Excreted/Secreted Products Induce a Defined MicroRNA Profile that Modulates Inflammatory Properties of Macrophages by Diana Martínez-Saucedo, Juan de Dios Ruíz-Rosado, César Terrazas, Blanca E. Callejas, Abhay R. Satoskar, Santiago Partida-Sánchez, Luis I. Terrazas

“…To further define the anti-inflammatory effect of TcES in the response of LPS-stimulated macrophages, we performed transcriptomic array analyses of mRNA and microRNA to evaluate their levels. Although the addition of TcES to LPS-stimulated BMDM induced modest changes in the inflammatory mRNA profile, it induced the production of mRNAs associated with the activation of different receptors, phagocytosis, and M2-like phenotype. …”
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Lycium barbarum Polysaccharides Promote Maturity of Murine Dendritic Cells through Toll-Like Receptor 4-Erk1/2-Blimp1 Signaling Pathway by Xiangguo Duan, Yaru Lan, Xiaoyu Zhang, Shaozhang Hou, Jian Chen, Bin Ma, Yuhan Xia, Chunxia Su

“…Furthermore, we found that LBP could increase the mRNA and protein expression of TLR4, p38, Erk1/2, JNK, and Blimp1 signal molecules. …”
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The Effect of Chromium Picolinate Supplementation on the Pancreas and Macroangiopathy in Type II Diabetes Mellitus Rats by Shan Huang, Wenfang Peng, Xiaohong Jiang, Kan Shao, Lili Xia, Yubin Tang, Jiayin Qiu

“…In addition, the levels of serum NO and insulin were significantly increased and the serum levels of HbA1C, AGES, APN, and apelin were significantly decreased in groups 4 and 5 compared with group 2. The mRNA expression of APN and apelin in groups 4 and 5 was also recovered to the normal level. …”
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Genetic Characteristics of the Rat Fibroblast Cell Line Rat-1 by Thomas Liehr, Stefanie Kankel, Eva Miriam Buhl, Sarah K. Schröder-Lange, Ralf Weiskirchen

“…Additionally, we analyzed the transcriptome of Rat-1 cells through mRNA sequencing (mRNA-Seq) using next-generation sequencing (NGS). …”
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大鼠放射性口腔黏膜炎病损组织细胞因子检测及意义

“…【目的】 检测大鼠放射性口腔黏膜炎形成过程中病损局部组织内细胞因子,探讨其动态变化规律及意义65377;【方法】 获取接受单次30 Gy X线照射后5 d65380;8 d65380;14 d的大鼠口腔黏膜组织,采用抗体芯片技术检测19种细胞因子;荧光定量PCR检测8种炎性相关因子的mRNA水平;ELISA定量检测IL-1α65380;IFN-γ65380;TIMP-165377;【结果】 抗体芯片技术检测到,照射后Frac65380;IL-465380;IL-665380;IL-1065380;TNF-α 5种细胞因子水平下降;IL-1β65380;LIX65380;VEGF65380;β-NGF 4种细胞因子照射前后水平无明显差异;照射后水平上升幅度 < 2倍的有CINC-265380;GM-CGF65380;Leptin65380;MCP-1和MIP-3α 5种细胞因子,上升幅度≥2倍的有CINC-365380;INF-γ65380;IL-1α和TIMP-1 4种细胞因子65377;Real-time PCR检测到抗凋亡相关基因Bcl-2,以及IL-665380;TNF-α65380;VEGF及IL-1β的mRNA表达水平下降;而促凋亡相关基因Bax65380;中性粒细胞局部募集相关基因CINC和TLR-9表达上升65377; IL-1α65380;IFN-γ65380;TIMP-1的ELISA检测结果与前两种检测结果一致65377;【结论】 大鼠抗体芯片和荧光定量PCR65380;ELISA检测结果互相佐证了病损组织内的细胞因子在口腔黏膜炎发生65380;发展65380;演进中呈现分泌受阻和表达增加的动态变化并发挥调控作用65377;…”
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Long-Term Environmental Enrichment Relieves Dysfunctional Cognition and Synaptic Protein Levels Induced by Prenatal Inflammation in Older CD-1 Mice by Zhe-Zhe Zhang, Li-Ping Zeng, Jing Chen, Yong-Fang Wu, Ya-Tao Wang, Lan Xia, Qi-Gang Yang, Fang Wang, Gui-Hai Chen

“…The LPS-E group exhibited better cognition and lower protein and mRNA levels of Arc and Syt1 than mice in the LPS group of the same age. …”
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Exploring the potential effect and mechanisms of protocatechuic acid on human hair follicle melanocytes by Li Bo, Tan Jun, Zou Bosheng, Liu Xiaojia, Yu Yiling

“…Normal primary HFM were isolated and cultured till logarithmic period of second passage, then treated with different concentrations of PCA (0.1–200 μmol L−1) to study the cell proliferation, melanin contents, tyrosinase activity and protein and mRNA expression of melanogenic genes (tyrosinase-related protein 1 (TRP-1), tyrosinase-related protein 2 (TRP-2), and microphthalmia-associated transcription factor (MITF)) in the cultured HFM. …”
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