Altered Autophagy-Associated Genes Expression in T Cells of Oral Lichen Planus Correlated with Clinical Features by Ya-Qin Tan, Jing Zhang, Ge-Fei Du, Rui Lu, Guan-Ying Chen, Gang Zhou

“…The present study mainly investigated the mRNA expression of autophagy-associated genes in peripheral blood T cells of OLP patients and evaluated correlations between their expression and the clinical features of OLP. …”
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Relationship between the CUBN and the MIA3 gene copy number variation and growth traits in different cattle breeds by Yue Han, Jiwei Liu, Congcong Zhang, Ming Sun, Xuanyu Li, Hongliang Liu, Shengnan Li, Yongchao Zhu, Ruidong Li, Xiaotong Luo, Yumin Zhao, Jian Wu

“…MIA3 showed no CNV in CYH and YB cattle, and only one deletion type occurred in YH cattle. CUBN and MIA3 mRNA have different expression patterns in different cattle breeds and tissues. …”
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KIF23基因沉默对肝癌HepG2细胞增殖、迁移及侵袭的影响 by 刘素娟, 柏明军, 周楚人, 陈俊伟, 黄明声

“…【方法】化学合成针对KIF23基因的siRNA，通过脂质体转染至HepG2细胞中，应用qRT-PCR 和Western blot法检测 转染后HepG2细胞中KIF23 mRNA和蛋白表达变化。采用CCK-8法和平板克隆法检测KIF23沉默后对HepG2细胞 增殖的影响，并通过细胞划痕实验和Transwell侵袭实验分别检测KIF23沉默后对迁移和侵袭能力的影响。…”
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Anti-Tumor Effect of Rutin on Human Neuroblastoma Cell Lines through Inducing G2/M Cell Cycle Arrest and Promoting Apoptosis by Hongyan Chen, Qing Miao, Miao Geng, Jing Liu, Yazhuo Hu, Lei Tian, Jingkun Pan, Yi Yang

“…In the meantime, the MYCN mRNA level and the secretion of TNF-α were inhibited. …”
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The Therapeutic Effects and Possible Mechanism of Pranoprofen in Mouse Model of Corneal Alkali Burns by Minting Chen, Abdirahman Abdinasir Gureeye, Yacouba Cissé, Lang Bai

“…Real-time RT-PCR and western blot were also performed to detect the mRNA and protein expression of NLRP3, IL-1β/p17, and matrix metallopeptidase MMP-13. …”
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Ningxiang pig-derived Lactobacillus reuteri improves the gut health of weaned piglets by regulating intestinal barrier function and cytokine profiles by Qian Xie, Mei Yang, Qing Duanmu, Meng Kang, Jing Wang, Bi E Tan

“…Additionally, L. reuteri notably enhanced the villus height-to-crypt depth ratio in the ileum (P < 0.05) and increased mRNA expression of zonula occludens-1 (ZO-1), Claudin-1, and ZO-1 in ileal tissue (P < 0.05). …”
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Food Intake and Core Body Temperature of Pups and Adults in a db Mouse Line Deficient in the Long Form of the Leptin Receptor without Misty Mutation by Wijang Pralampita Pulong, Miharu Ushikai, Emi Arimura, Masaharu Abe, Hiroaki Kawaguchi, Masahisa Horiuchi

“…Additionally, db/db mice displayed significantly lower mRNA levels of brown adipose tissue uncoupling protein 1 at both ages. …”
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TMEM101 expression and its impact on immune cell infiltration and prognosis in hepatocellular carcinoma by Lingyun Kuang, Yilin Pang, Quangang Fang

“…This study conducted an analysis of TMEM101 mRNA expression and its correlation with clinical outcomes in HCC patients using RNA sequencing data from various open databases. …”
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Prognostic Relevance and Function of MSX2 in Colorectal Cancer by Jiancheng Liu, Huaying An, Wei Yuan, Qiang Feng, Lianzhen Chen, Jie Ma

“…Compared with adjacent tissues, the expression of MSX2 was higher in tumor tissues in both mRNA and protein levels (P<0.01). Kaplan-Meier survival analysis showed that high mRNA expression of MSX2 was associated with short survival time (P=0.013). …”
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Effects of asiaticoside on the model of gestational diabetes mellitus in HTR-8/svneo cells via PI3K/AKT pathway by Zhilan Hu, Ya Long, Xiangyue Li, Zhiqin Jia, Mingyan Wang, Xuemei Huang, Xiaolan Yu

“…The cell proliferation rate, migration rate, mRNA levels and protein expressions of PI3K, AKT2, mTORC1, and GLUT4 in the HG group were significantly lower than those in the control group, which were significantly increased in the HG + AS group (p < 0.05).Conclusions AS can facilitate the cell proliferation and migration in the cell model of GDM, and might play a role in GDM treatment via PI3K/AKT pathway.…”
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强直性脊柱炎患者外周血单个核细胞亲环素Ａ的表达及意义

“…Ｗｅｓｔｅｒｎｂｌｏｔ及半定量逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）检测ＰＢＭＣ中ＣｙＰＡ蛋白及ｍＲＮＡ表达，Ｐｅａｒｓｏｎ相关分析了解ＡＳ患者ＰＢＭＣ中ＣｙＰＡ蛋白水平与病情活动性指标的相关性。…”
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脂多糖通过p38/MAPK通路对巨噬细胞自噬的调控作用 by 赖义明

“…qRT-PCR方法检测各组中与细胞自噬相关的基因Atg5,Atg7,LC3-Ⅱ和Bnip3 mRNA表达水平的改变?利用Western blotting检测LC3-Ⅱ?p-P38?P38蛋白在各组中的表达情况,以评价LPS激活巨噬细胞自噬过程中p38/MAPK通路的作用?…”
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The functional insight into the genetics of cardiovascular disease: results from the post-GWAS study by L. O. Bryzgalov, E. E. Korbolina, I. S. Damarov, T. I. Merkulova

“…A significant impact on binding sites of thirteen TFs including those involved in blood cells formation, hematopoiesis, macrophage function, inflammation, and vasoconstriction was found in K562 cells. 21 rSNP gene targets and 5 partners predicted by PPI were enriched for spliceosome and endocytosis KEGG pathways, endosome sorting complex and mRNA splicing REACTOME pathways. Related Gene Ontology terms included mRNA splicing and processing, endosome transport and protein catabolic processes. …”
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Semen extender triggers a mild physiological inflammatory response in the uterus without disrupting sperm-uterine immune crosstalk in vitro in cattle by Malinda HULUGALLA, Alireza MANSOURI, Elham WAEHAMA, Ihshan AKTHAR, Akio MIYAMOTO

“…When combined with sperm, SE did not enhance the protein or mRNA expression of these markers, except for IL1B and CXCL8. …”
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Structural Characterization of Polygonum divaricatum L. Polysaccharide and Its Inhibitory Effect on Lipopolysaccharide-Induced Inflammation in RAW264.7 Cells by CUI Yanyan, YUAN Yongxu, GUO Mingkun, HE Wenbing, LI Ming, PEI Shichun, LI Dajun

“…PSPDL inhibited the release of inflammatory factors and related gene mRNA expression, ameliorated oxidative stress, down-regulated the protein expression levels of p38, p-p38, IκB-α, p65, and p-p65. …”
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PD-L1 Blockade Attenuated Sepsis-Induced Liver Injury in a Mouse Cecal Ligation and Puncture Model by Weimin Zhu, Rui Bao, Xiaohua Fan, Tianzhu Tao, Jiali Zhu, Jiafeng Wang, Jinbao Li, Lulong Bo, Xiaoming Deng

“…Our results indicated that there was a significant increase of PD-L1 expression in liver after CLP challenge compared to sham-operated controls, in terms of levels of mRNA transcription and immunohistochemistry. Anti-PD-L1 antibody significantly alleviated the morphology of liver injury in CLP mice. …”
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Interleukin-29 Enhances Synovial Inflammation and Cartilage Degradation in Osteoarthritis by Lingxiao Xu, Qiuyue Peng, Wenhua Xuan, Xiaoke Feng, Xiangqing Kong, Miaojia Zhang, Wenfeng Tan, Meilang Xue, Fang Wang

“…Furthermore, recombinant IL-29 augmented the mRNA expression of IL-1β, IL-6, IL-8, and matrix-metalloproteinase-3 (MMP-3) in OA FLS and increased cartilage degradation when ex vivo OA cartilage explant was coincubated with OA FLS. …”
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ACE 抑制剂Ramipril对大鼠急性心肌梗死后心肌细胞凋亡 和左室重构的影响

“…循环血中AngⅡ含量(P < 0.05)?对照组Fas-L基因mRNA表达明显增加(P < 0.05),雷米普利显著抑制其表达(P < 0.05)?心梗2周后假手术组?…”
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ISU201 Enhances the Resolution of Airway Inflammation in a Mouse Model of an Acute Exacerbation of Asthma by Yuka Hiroshima, Linda Garthwaite, Kenneth Hsu, Hyouna Yoo, Sang-Ho Park, Carolyn L. Geczy, Rakesh K. Kumar, Cristan Herbert

“…ISU201 was administered to mice 2 and 8 hours later, while pulmonary inflammation and expression of mRNA for chemokines and proinflammatory cytokines were assessed after 4, 12, and 24 hours. …”
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NF-κB-94ins/del ATTG Genotype Contributes to the Susceptibility and Imbalanced Th17 Cells in Patients with Immune Thrombocytopenia by Jie Yu, Mingqiang Hua, Xueyun Zhao, Rui Wang, Chaoqing Zhong, Chen Zhang, Ruiqing Wang, Guosheng Li, Na He, Ming Hou, Daoxin Ma

“…Compared with controls, NF-κB gene expression was significantly decreased and WW or WD genotype ITP patients displayed higher mRNA expression than DD individuals. Similarly, the mRNA expression of NLRP3 was also increased in the WW genotype. …”
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