Quercetin attenuates cisplatin-induced fatigue through mechanisms associated with the regulation of the HPA axis and MCP-1 signaling by Cheng-Hung Chuang, Yu-An Tai, Ting-Jing Wu, Ying-Jui Ho, Shu-Lan Yeh, Shu-Lan Yeh

“…OQ and IQ also decreased CDDP-induced plasma levels of MCP-1 as well as the mRNA expression of MCP-1 and CCR2 in the brain stem. …”
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结缔组织生长因子介导高糖诱导肾小管 上皮细胞肥大的机制

“…【目的】 研究高糖诱导肾小管上皮细胞肥大的作用途径及可能机制65377;【方法】 HK-2细胞分正常对照组(培养基含葡萄糖1 g/L),等渗对照组(葡萄糖1 g/L + 甘露醇3.5 g/L)和高糖组(葡萄糖4.5 g/L)培养65377;收集各组培养至24 h,48 h,96 h的细胞,检测结缔组织生长因子(CTGF)65380;p27kip的mRNA水平65380;CTGF65380;p27kip的蛋白水平65380;细胞增殖活力65380;细胞周期分布及细胞总蛋白含量65377;另外检测各组培养30 min65380;1 h65380;24 h65380;48 h时的磷酸化细胞外信号调节激酶1/2(p-ERK1/2)水平65377;每个检测指标设3个重复样本65377; 【结果】 高糖刺激可引起HK-2细胞的CTGF65380;p27kip的mRNA及蛋白表达水平显著升高,ERK1/2磷酸化激活,阻滞于G1期的细胞显著增多,细胞增殖活力受抑制,细胞肥大主要指标胞内蛋白总含量进行性增高65377; 【结论】 证实了高糖可通过上调CTGF激活ERK1/2信号转导,从转录水平上调p27kip,从而使增殖细胞阻滞于G1期诱导细胞肥大65377;…”
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Long-Term Treatment with Atypical Antipsychotic Iloperidone Modulates Cytochrome P450 2D (CYP2D) Expression and Activity in the Liver and Brain via Different Mechanisms by Przemysław J. Danek, Władysława A. Daniel

“…Chronic iloperidone did not change the brain <i>CYP2D4</i> mRNA levels, except in the striatum, where they were significantly increased. …”
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A comprehensive analysis to reveal the underlying molecular mechanisms of natural killer cell in thyroid carcinoma based on single-cell RNA sequencing data by Xiaoqiong Li, Kejiang Wang, Jiaxin Liu, Yan Li

“…Hub genes associated with NK cell were identified by Pearson correlation analysis. The mRNA-miRNA-lncRNA and transcription factors (TF) networks were constructed and the drug was predicted. …”
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Microbial signatures in head and neck squamous cell carcinoma: an in silico study by Loganathan KAVITHA, Manogaran KUZHALMOZHI, Jayaseelan VIJAYASHREE PRIYADHARSINI, Arunachalam ARUN KUMAR, Krishna Mohan Rao UMADEVI, Kannan RANGANATHAN

“…The differential expression of the host’s mRNA transcripts and proteins associated with tumor microbiome were analyzed using The University of Alabama at Birmingham Cancer data analysis (UALCAN) and Clinical Proteomic Tumor Analysis Consortium (CPTAC) websites. …”
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Constructing the biomolecular networks associated with diabetic nephropathy and dissecting the effects of biomolecule variation underlying pathogenesis by Zi-Han Wang, Qi Dong, Qian Yan, Wan-Rong Yu, Dan-Dan Zhang, Ran Yi

“…In the second step, we investigated the differential expression and co-expression of hub nodes in diseased and normal individuals, identifying lncRNA-mRNA relationships implicated in disease regulation. …”
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Role of Toll-like receptor 2 during infection of Leptospira spp: A systematic review. by Chamila Kappagoda, Indika Senavirathna, Thilini Agampodi, Suneth Buddhika Agampodi

“…We assessed the direct TLR2 expression and indirect TLR2 involvement via the secretion/mRNA expression of immune effectors during leptospirosis. …”
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实时荧光定量PCR检测原发性肝癌中PRL-2基因的表达 by 程超, 郭爱林, 巫国勇, 谢春玲, 吴伟康

“…【目的】建立TaqMan实时荧光定量逆转录聚合酶链反应（RT-PCR）法检测肝细胞再生磷酸酯酶2（PRL-2）mRNA表达水平，并应用该法检测原发性肝细胞癌中PRL-2基因的表达。【方法】构建含PRL-2基因开放阅读框架的T载体．制作标准曲线。…”
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Brucella induces an unfolded protein response via TcpB that supports intracellular replication in macrophages. by Judith A Smith, Mike Khan, Diogo D Magnani, Jerome S Harms, Marina Durward, Girish K Radhakrishnan, Yi-Ping Liu, Gary A Splitter

“…Consistent with previous reports, XBP1 mRNA splicing was largely MyD88-dependent. However, up regulation of CHOP, and ERdj4 was completely MyD88 independent. …”
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N6-Methyladenosine in RNA and DNA: An Epitranscriptomic and Epigenetic Player Implicated in Determination of Stem Cell Fate by Pengfei Ji, Xia Wang, Nina Xie, Yujing Li

“…N6-Methyladenosine modification in DNA (m6dA), mRNA (m6A), tRNA, and other noncoding RNAs has been defined as another important epigenetic and epitranscriptomic marker in eukaryotes in recent years. …”
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MFG-E8 Exerts Neuroprotection in Neural Stem Cells Induced by Anesthetic Sevoflurane via Regulating the PI3K/AKT Pathways by Minmin Cai, Liufang Sheng

“…The cell viability and apoptosis affected by rhMFG-E8 were tested by MTT and flow cytometry analysis, respectively. Then, the mRNA expression of MFG-E8 was detected by qRT-PCR. …”
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Anticolitic Effect of Berberine in Rat Experimental Model: Impact of PGE2/p38 MAPK Pathways by Li Jia, Kuijin Xue, Junheng Liu, Ola A. Habotta, Lianhai Hu, Ahmed E. Abdel Moneim

“…Notable downregulation of mRNA expression of p38 MAPK and increased protein expression of TGF-β were achieved by BER treatment. …”
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Inhibition of Cathepsin S Produces Neuroprotective Effects after Traumatic Brain Injury in Mice by Jianguo Xu, Handong Wang, Ke Ding, Xinyu Lu, Tao Li, Jiawei Wang, Chunxi Wang, Jian Wang

“…In this study, we investigated the time course of CatS protein and mRNA expression and the cellular distribution of CatS in a mouse model of traumatic brain injury (TBI). …”
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New Insights into Behçet’s Syndrome Metabolic Reprogramming: Citrate Pathway Dysregulation by Anna Santarsiero, Pietro Leccese, Paolo Convertini, Angela Padula, Paolo Abriola, Salvatore D’Angelo, Faustino Bisaccia, Vittoria Infantino

“…Real-time PCR experiments were performed on PBMCs to quantify SLC25A1 and ACLY mRNA levels. Data processing through the Kruskal-Wallis test and Dunn’s multiple comparison test as post hoc showed higher SLC25A1 and ACLY mRNA levels in BS patients compared to those in healthy controls. …”
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小鼠胚胎干细胞源表皮样细胞 β1 整合素的表达与序列分析 by 张仁礼

“…【方法】用双层培养板将小鼠胚胎干细胞与人羊膜共培养4～5d,显微镜下观察其形态分化,分别用免疫组化法和Actin内参照逆转录聚合酶链反应(reversetranscriptionpolymerasechainreaction,RT-PCR)定量检测β1整合素蛋白和mRNA的表达情况,并对RT-PCR产物进行测序,对照组未加羊膜。【结果】与人羊膜共培养4～5d后,ES细胞分化为表皮细胞样的单层细胞,细胞排列紧密,呈多边形;免疫组化结果显示诱导4d后部分细胞呈β1整合素阳性;RT-PCR结果显示实验组β1整合素mRNA有较强的表达;对照组细胞形态各异,不能形成细胞单层,几乎不表达β1整合素,测序及同源性比对结果表明两条扩增序列分别与β1整合素具有100%的同源性。…”
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Resistin Regulates Pituitary Lipid Metabolism and Inflammation In Vivo and In Vitro by F. Rodriguez-Pacheco, M. G. Novelle, M. J. Vazquez, E. Garcia-Escobar, F. Soriguer, G. Rojo-Martinez, E. García-Fuentes, M. M. Malagon, C. Dieguez

“…Here, by a combination of in vivo and in vitro experimental models, we demonstrated that central acute and chronic administration of resistin enhance mRNA levels of the lipid metabolic enzymes which participated on lipolysis and moreover inhibiting mRNA levels of the lipid metabolic enzymes involved in lipogenesis. …”
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The Protective Effect of Low-Dose Ethanol on Myocardial Fibrosis through Downregulating the JNK Signaling Pathway in Diabetic Rats by Ying Yu, Xian-Jie Jia, Wei-ping Zhang, Ting-ting Fang, Jie Hu, Shan-Feng Ma, Qin Gao

“…Morphology of collagen in myocardial tissue was observed with Masson’s trichrome staining method and collagen volume fraction (CVF) was analysed. The mRNA expression of ALDH2 was assessed with Real-Time PCR. …”
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Bone Marrow Mesenchymal Stem Cells Decrease the Expression of RANKL in Collagen-Induced Arthritis Rats via Reducing the Levels of IL-22 by Fang Li, Xin Li, Guiyan Liu, Chong Gao, Xiaofeng Li

“…In addition, the expression of RANKL mRNA was measured by RT-PCR in the fibroblast-like synoviocytes (FLSs), cultured with IL-22 in vitro, which were delivered from the joints of CIA rats treated with or without MSCs. …”
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Vector‐Free Deep Tissue Targeting of DNA/RNA Therapeutics via Single Capacitive Discharge Conductivity‐Clamped Gene Electrotransfer by Jeremy L. Pinyon, Georg vonJonquieres, Stephen L. Mow, Amr Al Abed, Keng‐Yin Lai, Mathumathi Manoharan, Edward N. Crawford, Stanley H. Xue, Sarah Smith‐Moore, Lisa J. Caproni, Sarah Milsom, Matthias Klugmann, Nigel H. Lovell, Gary D. Housley

“…Proof‐of‐concept studies for “single capacitive discharge conductivity‐clamped gene electrotransfer” (SCD‐CC‐GET) deep tissue delivery of naked DNA and mRNA in the mouse hindlimb skeletal muscle achieve stable (>18 month) expression of luciferase reporter synthetic DNA, and mRNA encoding the reporter yield rapid onset (<3 h) high transient expression for several weeks. …”
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羟基喜树碱脂质体调节SphK1/S1P信号通路对心力衰竭大鼠心肌纤维化的影响 by 李思阳, 李更东, 闫宇辉

“…结果与对照组比较，模型组大鼠心肌组织病理损伤及纤维化严重，LVESD、LVEDD、TNF-α、IL-6水平、<italic>TGF-β1</italic>、<italic>Collagen I</italic>、<italic>Collagen Ⅲ</italic> mRNA表达及SphK1、S1P蛋白表达升高，LVEF降低（<italic>P</italic>＜0.05）；与模型组比较，HCPT组、LHCPT组、卡托普利组大鼠心肌组织病理损伤及纤维化减轻，LVESD、LVEDD、TNF-α、IL-6水平、<italic>TGF-β1</italic>、<italic>Collagen Ⅰ</italic>、<italic>Collagen Ⅲ</italic> mRNA表达及SphK1、S1P蛋白表达降低，LVEF升高（<italic>P</italic>＜0.05）；与LHCPT组比较，LHCPT+K6PC-5组大鼠心肌组织病理损伤及纤维化加剧，LVESD、LVEDD、TNF-α、IL-6水平、<italic>TGF-β1</italic>、<italic>Collagen Ⅰ</italic>、<italic>Collagen</italic> <italic>Ⅲ</italic> mRNA表达及SphK1、S1P蛋白表达升高，LVEF降低（<italic>P</italic>＜0.05）。…”
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