Identification of the Role of Mume fructus in Treating Nocturnal Asthma Based on Network Pharmacology and In vitro Investigation by Ling-Ling Tang, Yan-Qiu Xu, Ying Yang, Xian-Hong Sun, Bo-Han Wang, Xiao-Lu Shi, Li Liu

“…Mume fructus counteracted LPS-stimulated mRNA and protein expression of IL-6. In addition, Mume fructus treatment prevented the LPS-induced phosphorylation of NF-κB and MAPK pathways, as well as the nuclear translocation of NF-κB p-p65 in the 16HBE and RAW264.7 cells. …”
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Network pharmacology combined with experimental verification for exploring the potential mechanism of phellodendrine against depression by Lili Hu, Na Wu, Jue Wang, Donghui Cai

“…PHE affected the levels of IL6 and IL1β with different concentrations. The mRNA levels of CHRM1, HTR1A and key targets of the PI3K/Akt signalling pathway were also influenced. …”
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Translational regulation of PKD1 by evolutionarily conserved upstream open reading frames by Lei Chen, Xia Gao, Xiangshen Liu, Ye Zhu, Dong Wang

“…Our analyses demonstrate that PKD1 mRNA contains two evolutionarily conserved translation-inhibitory uORFs. uORF1 is translatable, and uORF2 is likely not translatable. …”
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The Role of Epoxyeicosatrienoic Acids in Diabetes Mellitus-Induced Impaired Vascular Relaxation of Aortic Rings in Ovariectomized Sprague-Dawley Rats by Dragan Manojlović, Ana Stupin, Anita Matić, Zrinka Mihaljević, Sanja Novak, Ines Drenjančević

“…MS-PPOH did not reduce AChIR in OVX-DM rats as it did in OVX-CTR ones. CYP4a3 mRNA expression in OVX-DM rats was significantly lower compared to that in the OVX-CTR group. …”
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HuR Plays a Positive Role to Strengthen the Signaling Pathways of CD4+ T Cell Activation and Th17 Cell Differentiation by Shiguang Yu, Morgan Tripod, Ulus Atasoy, Jing Chen

“…Our work showed that, mechanistically, HuR positively promoted CD3g expression by binding its mRNA and enhanced the expression of downstream adaptor Zap70 and Malt1 in activated CD4+ T cells. …”
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The different response of PM2.5 stimulated nasal epithelial spheroids in control, asthma and COPD groups by Paulina Misiukiewicz-Stępień, Elwira Zajusz-Zubek, Katarzyna Górska, Rafał Krenke, Magdalena Paplińska-Goryca

“…Results The common pattern for healthy asthma and COPD epithelium inflammatory response to PM2.5 stimulation include the increase in IL-1β, IL-6, IL-8 mRNA expression, and secretion of IL-6. Asthmatic spheroids produced higher amount of TNF-α and IL-8, whereas COPD spheroids expressed increased mRNA level of MUC5AC and decreased level of MMP7. …”
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雷公藤内酯上调PTEN 信号通路减轻异丙肾上腺素诱导的 慢性心力衰竭大鼠心室重构 by 刘茂, 陈剑, 姚婕榕, 谭广毅, 伍卫

“…采用Iso诱导 CHF大鼠心室重构模型，TPL组和Cap组分别给予TPL（每天20 μg/kg）或Cap（每天15 mg/kg）连续灌胃6周，测量大鼠左室收 缩末期内径（LVIDs）、左室舒张末期内径（LVIDd）和心室体质量指数（VW/BW）；行Masson染色和Ⅰ型胶原免疫组化，计算心 肌胶原容积分数（CVF）和血管周围胶原面积（PVCA），评价CHF大鼠心肌纤维化程度；行Western Blot和实时荧光PCR检测 大鼠心肌组织PTEN mRNA和蛋白表达水平。【结果】TPL组、Cap组与Iso组相比较，TPL和Cap治疗可降低CHF大鼠LVIDd、 LVIDs、VW/BW、CVF和PVCA，CHF大鼠心肌纤维化程度明显减轻，PTEN mRNA及蛋白表达水平明显升高。…”
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Conditioned Media from Adipose-Tissue-Derived Mesenchymal Stem Cells Downregulate Degradative Mediators Induced by Interleukin-1β in Osteoarthritic Chondrocytes by Julia Platas, Maria Isabel Guillén, María Dolores Pérez del Caz, Francisco Gomar, Vicente Mirabet, Maria José Alcaraz

“…CM enhanced type II collagen expression in OA chondrocytes while decreasing matrix metalloproteinase (MMP) activity in cell supernatants as well as the levels of MMP-3 and MMP-13 proteins and mRNA in OA chondrocytes stimulated with interleukin- (IL-) 1β. …”
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Downregulation of Thromboxane A2 Receptor Occurs Mainly via Nuclear Factor-KappaB Signaling Pathway in Rat Renal Artery by Yaping Zhang, Man Mi, Yan-Hua Xie, Si-Wang Wang, Lars Edvinsson, Cang-Bao Xu

“…Downregulation of TP receptors was monitored using myograph (contractile function), real-time PCR (receptor mRNA), and immunohistochemistry (receptor protein). …”
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RhoA/Rho激酶信号通路在血管紧张素Ⅱ刺激心肌成纤维细胞增殖和胶原合成中的作用 by 汪祥海, 伍卫, 杨军, 方昶, 耿登峰, 黄至斌

“…采用四氮唑盐比色法测定细胞增殖,羟脯氨酸法测定CFBs胶原含量,RT-PCR检测RhoA/Rho激酶mRNA的表达,Western blot检测肌球蛋白结合亚基磷酸化(phosphorylation of myosin-binding subunit,MBS-P)表达作为Rho激酶功能活化的标志。…”
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Optimization of the intron sequences combined with the CMV promoter increases recombinant protein expression in CHO cells by Ting-Ting Xu, Yan-Fang Wang, Jing-Jia Yuan, Chun-Liu Mi, Shao-Lei Geng, Xiao-yin Wang, Tian-Yun Wang

“…The qPCR results indicated that the mRNA level of transgene increased through optimizing intron sequences combined with the CMV promoter. …”
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Expression and Secretion of CXCL-8 and CXCL-10 From Mycobacterium Bovis BCG-Infected Human Epithelial Cells: Role of IL-4 by Patricia Méndez-Samperio, Elena Miranda, Abraham Vázquez

“…We found that, although M. bovis BCG induced gene expression of CXCL-8 and CXCL-10 in M. bovis BCG-infected human epithelial cells, CXCL-8 mRNA level was significantly reduced by IL-4, whereas no significant effect of IL-4 was observed on CXCL10 mRNA level. …”
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Methylation Status of Vitamin D Receptor Gene Promoter in Benign and Malignant Adrenal Tumors by Catia Pilon, Andrea Rebellato, Riccardo Urbanet, Vincenza Guzzardo, Rocco Cappellesso, Hironobu Sasano, Ambrogio Fassina, Francesco Fallo

“…We previously showed a decreased expression of vitamin D receptor (VDR) mRNA/protein in a small group of adrenocortical carcinoma (ACC) tissues, suggesting the loss of a protective role of VDR against malignant cell growth in this cancer type. …”
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高成瘤性前列腺癌细胞亚克隆细胞株在反基因治疗中的应用

“…实时荧光定量逆转录PCR(qRT-PCR)检测肿瘤组织AR mRNA表达;免疫组化法检测AR,PSA,Ki67蛋白的表达;TUNEL法检测细胞凋亡?【结果】 TF0治疗组的抑瘤率为63.16%,AR mRNA的表达水平(39.16 ± 3.21)%,Ki67的阳性表达率(25.43 ± 2.01)%,凋亡指数(35.76±2.35)%,与对照组相比差异有统计学意义(P < 0.05)?…”
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慢病毒携带shRNA抑制CR-1基因表达对 CNE-2细胞特性的影响

“…【目的】 应用慢病毒介导的RNA干扰(RNAi)技术,有效沉默鼻咽癌细胞CNE-2的CR-1基因表达,研究CR-1对鼻咽癌细胞生物学行为的影响65377; 【方法】 构建针对CR-1的siRNA慢病毒表达载体[含H1启动子和绿色荧光蛋白(GFP)]65377;利用包装细胞293FT生产慢病毒,感染鼻咽癌细胞CNE-2, 流式筛选GFP阳性细胞65377;应用荧光定量PCR65380;Western blot检测病毒感染后CNE-2细胞中CR-1基因的mRNA和蛋白表达,获得干扰CR-1基因的细胞群65377;并用MTT法65380;平板克隆形成实验及Boyden侵袭小室观察CR-1干扰后对CNE-2细胞生物学行为的影响65377; 【结果】 PCR 和测序证实,成功构建了CR-1 shRNA的慢病毒载体pLVTHM-shCR-165377;荧光定量PCR 和Western blot结果表明,所获得的细胞中CR-1 mRNA及蛋白表达水平较阴性对照组65380;空白对照组均显著降低(P < 0.05)65377;CR-1干扰后的细胞生长明显减慢,克隆形成能力减弱,侵袭运动能力下降65377; 【结论】 通过RNAi技术阻断CR-1的表达,可抑制CNE-2细胞的生长65380;增殖65380;迁徙,提示CR-1在鼻咽癌的发生65380;发展过程中起重要作用65377;…”
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胰岛素样生长因子Ⅰ与透明质酸对人胚关节软骨细胞表型的影响 by 黄建荣, 刘尚礼, 宋卫东, 李卫平, 郑召民, 沈慧勇, 黄东生

“…【结果】IGF-Ⅰ 和HA 联合培养的第8 代细胞胞浆内含有丰富的粗面内质网和分泌小泡;RT-PCR 显示Ⅱ 型胶 原mRNA 表达阳性而Ⅰ 型胶原mRNA 表达阴性;甲苯胺蓝染色显示细胞浆内有大量的紫色异染颗粒;Ⅱ 型胶原和agg recan 免疫组化见80 %～ 92 %的细胞染色呈阳性或强阳性, 平均灰度分别为85.92 和116.23, 均显著低于对照组, 说明处理组染色 较对照组增强。…”
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Identification of KRT16 and ANXA10 as cell cycle regulation genes for lung adenocarcinoma based on self-transcriptome sequencing of surgical samples and TCGA public data mining by Wen-jian Liu, Jia-pan Shen, Ren-quan Zhang, Xiao-yun Fan

“…The expression was validated at mRNA and protein levels, and the gene knockdown was used to examine the impact and underlying mechanisms on lung cancer cells. …”
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Naringenin, a Food Bioactive Compound, Reduces Oncostatin M Through Blockade of PI3K/Akt/NF-κB Signal Pathway in Neutrophil-like Differentiated HL-60 Cells by Na-Ra Han, Hi-Joon Park, Seong-Gyu Ko, Phil-Dong Moon

“…While exposure to granulocyte-macrophage colony-stimulating factor (GM-CSF) induced elevated OSM release and mRNA expression, the elevated OSM release and mRNA expression were diminished by the addition of NAR in dHL-60 cells. …”
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Increased nuclear expression of DNA damage inducible transcript 4 can serve as a potential prognostic biomarker in patients with gliomas: a study based on data mining and experimen... by Alireza Sadeghipour, Fahimeh Fattahi, Zahra Madjd, Fatemeh Tajik, Farnoosh Sedaghati, Leili Saeednejad Zanjani

“…Materials and methods The expression of DDIT4 at the mRNA level and its potential role as a prognostic biomarker in gliomas were analyzed using the GEPIA tool. …”
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The highly prolific phenotype of Lacaune sheep is associated with an ectopic expression of the B4GALNT2 gene within the ovary. by Laurence Drouilhet, Camille Mansanet, Julien Sarry, Kamila Tabet, Philippe Bardou, Florent Woloszyn, Jérome Lluch, Grégoire Harichaux, Catherine Viguié, Danielle Monniaux, Loys Bodin, Philippe Mulsant, Stéphane Fabre

“…The reduced FecL locus contained only two genes, insulin-like growth factor 2 mRNA binding protein 1 (IGF2BP1) and beta-1,4-N-acetyl-galactosaminyl transferase 2 (B4GALNT2), and we identified two SNP in complete linkage disequilibrium with FecL(L) . …”
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