Effect and Mechanism of Action of Pushing Method on Rats with Skeletal Muscle Overuse Injury based on Wnt/β-catenin Signaling Pathway by DUAN Miaomiao, HUANG Bo, RUAN Lei, MU Pangpang, CHEN Yiming, PENG Liang

“…The balance beam test was used to assess the motor function of the rats after skeletal muscle injury at 24 h after the end of modeling and 24 h after the end of the intervention, respectively; hematoxylin-eosin staining (HE) method was used to observe the pathological changes of the gastrocnemius muscle; EM-1400 transmission electron microscope was used to observe the ultrastructure of the skeletal muscle fibers; and fluorescence PCR was used to detect the myogenic regulatory factor myogenic differentiation antigen (Myogenic differentiation antigen (MyoD), myogenin (MyoG) and paired box 7 (Pax7) mRNA transcript levels were detected by fluorescence quantitative PCR; MyoD, MyoG, Pax7, Wnt3a, Axin2 and β-catenin protein expression levels were detected by Western blot. …”
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The methyl-CpG binding domain 2 regulates peptidylarginine deiminase 4 expression and promotes neutrophil extracellular trap formation via the Janus kinase 2 signaling pathway in e... by Biao Peng, Mu-Yun Yan, Yun-Rong Chen, Fei Sun, Xu-Dong Xiang, Da Liu

“…In both severe asthma mice and HL-60-differentiated neutrophil-like cells in vitro, inhibiting MBD2 reduced the mRNA and protein expression of JAK2 and PAD4, and inhibiting JAK2 reduced the expression of PAD4 mRNA and protein.Conclusion MBD2 regulates PAD4 expression through the JAK2 signaling pathway to promote NETs formation in mice with severe asthma. …”
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CCAAT/Enhancer-Binding Protein β Mediates Oxygen-Induced Retinal Neovascularization via Retinal Vascular Damage and Vascular Endothelial Growth Factor by Tingting Li, Xuan Cai, Xiangning Wang, Xueyan Zhang, Hui Zhang, Biwei Xu, Shiwei Li, Jianyan Hu, Qiang Wu

“…Reverse transcriptase-polymerase chain reaction (RT-PCR) and western blot analyses were used to measure mRNA and protein levels of C/EBP β and vascular endothelial growth factor (VEGF). …”
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Complement Receptors C5aR and C5L2 Are Associated with Metabolic Profile, Sex Hormones, and Liver Enzymes in Obese Women Pre- and Postbariatric Surgery by Reza Rezvani, Jessica Smith, Marc Lapointe, Picard Marceau, Andre Tchernof, Katherine Cianflone

“…Over 12 months postoperatively, liver enzymes in low C5L2/C5aR mRNA ratio group remained higher (ALP and ALT, P<0.05, AST and GGT, P<0.001 2-way-ANOVA). …”
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The value of exosomes suppressor of cytokine signaling 1， speckled protein 140 and calcyclin binding protein expressions in peritoneal dialysis effluent in the diagnosis of periton... by Han Xiao-li, Wang Jia-wei, Wei Zhi-feng, Wang Lin-lin, Guo Bao-zhu, Cao Yan-chun, Jin Yu-jie, Liu Sheng-jun

“…They were divided into PF group （n=38） and control group （non-PF， n=52） according to peritoneal tissue biopsy. The mRNA levels of exsome SOCS1， SP140 and CacyBP in peritoneal dialysis effluent were detected by quantitative reverse transcriptase polymerase chain reaction （qRT-PCR）. …”
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SLC1A5 is a key regulator of glutamine metabolism and a prognostic marker for aggressive luminal breast cancer by Lutfi H. Alfarsi, Rokaya El Ansari, Busra Erkan, Ali Fakroun, Madeleine L. Craze, Mohammed A. Aleskandarany, Kiu Wai Cheng, Ian O. Ellis, Emad A. Rakha, Andrew R. Green

“…In large BC cohorts, SLC1A5 mRNA (n = 9488) and SLC1A5 protein (n = 1274) levels were assessed and correlated their expression with clinicopathological features, molecular subtypes, and patient outcomes. …”
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The Impact of the Coexpression of MET and ESR Genes on Prognosticators and Clinical Outcomes of Breast Cancer: An Analysis for the METABRIC Dataset by Nehad M. Ayoub, Ghaith M. Al-Taani, Amer E. Alkhalifa, Dalia R. Ibrahim, Aymen Shatnawi

“…The ESR1 mRNA expression was significantly different among MET CNAs groups p<0.001. …”
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重新去极化对小脑颗粒神经元c-Jun 的影响 by 黄奕俊, 龚守芳, 邱鹏新, 苏兴文, 颜光美

“…【结果】复极化(repolarization , 5 mmol/ L K+)使小脑 颗粒神经元的c-jun mRNA 、c-Jun 总量及磷酸化水平迅速增加, 神经元存活率随后明显下降;复极化2 h 后重新去极化, c-jun m RNA、c-Jun 总量及磷酸化在2 h 内恢复至正常水平, 而神经元存活率维持在正常水平;JNK 活性在极化转换前后未改变。 …”
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Gene Expression of IGF1, IGF1R, and IGFBP3 in Epiretinal Membranes of Patients with Proliferative Diabetic Retinopathy: Preliminary Study by Dorota Romaniuk, Malgorzata W. Kimsa, Barbara Strzalka-Mrozik, Magdalena C. Kimsa, Adam Kabiesz, Wanda Romaniuk, Urszula Mazurek

“…Therefore, the present study focused on the assessment of IGF1, IGF1R, and IGFBP3 mRNA levels in ERMs and PBMCs from patients with PDR. …”
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Insulin Detemir Causes Lesser Weight Gain in Comparison to Insulin Glargine: Role on Hypothalamic NPY and Galanin by Mohammad Ishraq Zafar, Cuining Hu, Danfeng Liu, Raja Adeel Shafqat, Feng Gao

“…The expression of hypothalamic NPY and GAL at both mRNA and protein level were significantly lower (P<0.05) in the DM+DE group. …”
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Modifying Graft-versus-Host Disease in a Humanized Mouse Model by Targeting Macrophages or B-Cells by Marieke C. H. Hogenes, Suzanne van Dorp, Joyce van Kuik, Filipa R. P. Monteiro, Natalie ter Hoeve, Liane Guedes, Marijke R. van Dijk, Anton C. Martens, Roel A. de Weger

“…This may be related to a difference in the mRNA levels of TGF-β. Also, lower mRNA levels of Tregs in some organs were observed after Rituximab treatment, which contradicts that a higher number of Tregs would always be related to less severe GvHD. …”
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Pioglitazone Attenuates Vascular Fibrosis in Spontaneously Hypertensive Rats by Dengfeng Gao, Ning Ning, Guanghua Hao, Xiaolin Niu

“…Masson’s trichrome staining for collagen III and real-time RT-PCR analysis of collagen I, III and fibronectin mRNA indicated that pioglitazone significantly inhibited extracellular matrix production in the aorta. …”
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BioLake: an RNA expression analysis framework for prostate cancer biomarker powered by data lakehouse by Qiaowang Li, Yaser Gamallat, Jon George Rokne, Tarek A. Bismar, Reda Alhajj

“…In summary, BioLake offers a lightweight online tool to facilitate bioinformatic mRNA data analysis with the support of custom online data processing.…”
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系统性红斑狼疮患者骨髓基质细胞对造血干（祖）细胞的作用

“…④70%活动期SLE患者骨髓基质细胞有IFN-γmRNA的表达,而正常对照则未见有IFN-γmRNA的表达。【结论】活动期SLE患者骨髓基质细胞上清液对正常、自身骨髓造血干(祖)细胞的有抑制作用,这与其基质细胞存在高表达TNF-α、IFN-γ有关。…”
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Prophylactic effect of Kudingcha polyphenols on oxazolone induced colitis through its antioxidant capacities by Xingyao Long, Yanni Pan, Xin Zhao

“…Keywords: Kudingcha polyphenols, Oxazolone, Antioxidant, Colitis, mRNA expression…”
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Dulaglutide Protects Mice against Diabetic Sarcopenia-Mediated Muscle Injury by Inhibiting Inflammation and Regulating the Differentiation of Myoblasts by Fengyi Deng, Wenyan Wu, Xingyu Fan, Xing Zhong, Nuojin Wang, Yue Wang, Tianrong Pan, Yijun Du

“…High glucose inhibited the differentiation of C2C12 cells and decreased the mRNA and protein levels of myokines. Dulaglutide could reverse the differentiation state induced in C2C12 cells by high glucose.…”
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Sex-Differences in Renal Expression of Selected Transporters and Transcription Factors in Lean and Obese Zucker Spontaneously Hypertensive Fatty Rats by Andrea Babelova, Birgitta C. Burckhardt, Waja Wegner, Gerhard Burckhardt, Maja Henjakovic

“…The aim of this study was to identify sex-dependent expression of renal transporter mRNA in lean and obese Zucker spontaneously hypertensive fatty (ZSF1) rats and to investigate the interaction of the most altered transporter, organic anion transporter 2 (Oat2), with diabetes-relevant metabolites and drugs. …”
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靶向CIP2A的shRNA对人肠癌细胞生物学特征的影响 by 梁景琳, 叶俊文, 汪建平, 黄美近

“…【方法】采用RTPCR及Westernblot检测重组质粒转染肠癌HT29细胞前后mRNA和蛋白的变化；分别采用CCK8、Transwell小室 法、流式细胞术及裸鼠成瘤实验（n＝20）测定其对肠癌HT29细胞增殖、克隆形成、凋亡及侵袭能力的影响。…”
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HSP 90 在原发性肾病综合征发生和治疗中的意义

“…【结果】与对照组比较, INS 患者 HSP90 mRNA 表达水平明显增高; 而且 INS 患者中 GC抵抗组 HSP90 mRNA 表达显著高于 GC 敏感组 (P< 0.05)。…”
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Effects of Tocotrienol on Cardiovascular Risk Markers in Patients With Chronic Kidney Disease: A Randomized Controlled Trial by Liana Trugilho, Lívia Alvarenga, Ludmila Cardozo, Bruna Paiva, Jessyca Brito, Isis Barboza, Jonatas Almeida, Juliana dos Anjos, Pramod Khosla, Marcelo Ribeiro-Alves, Denise Mafra

“…Transcription factors NRF2 and NF-κB mRNA expressions remained unaltered in both groups. …”
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