Peripheral Expression of IL-6, TNF-α and TGF-β1 in Alzheimer's Disease Patients by Gamze Güven, Pınar Köseoğlu, Ebba Lohmann, Bedia Samancı, Erdi Şahin, Başar Bilgiç, Haşmet Ayhan Hanağası, Hakan Gürvit, Nihan Erginel-Ünaltuna

“…In addition, we evaluated the mRNA expressions of IL-6 and TNF-α in peripheral blood mononuclear cells from 31 AD patients and their respective controls (n=30) via quantitative real-time polymerase chain reaction. …”
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抑制NF-kB对大鼠肾小球系膜细胞血管紧张素Ⅱ 1型 受体表达的影响

“…【目的】 研究抑制NF-kB活性对SD大鼠系膜细胞血管紧张素Ⅱ1型受体mRNA表达的影响。【方法】 分离SD大鼠系膜细胞分为下列3组:正常葡萄糖培养组(5.6 mmol/L D-葡萄糖),高葡萄糖培养组(25 mmol/L),吡咯烷二硫氨基甲酸酯(pyrrolidinedithiocarbamate, PDTC) + 高葡萄糖培养组。…”
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β-glucan attenuates intestinal ischemia-reperfusion injury in mice by promoting glucagon-like peptide-1 secretion by WANG Wei, HAN Ben, SUN Lihua

“…While, the mRNA levels of the 3 molecules were obviously higher and the Chiu's score was lower in the II/R+BG group than the II/R group (P<0.05). …”
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不同病毒载量的乙型肝炎病毒在不同ALT状态下对肝星状细胞增殖、细胞周期及细胞分泌的影响 by 高鹏, 刘才周, 朱陇东, 李俊峰, 于海涛, 姚立琼

“…而在ALT异常状态下，特别是 ALT ≥2UNL组中，随着病毒载量的增加，HSC增殖加速，处于G0/G1期的明显减少，而处于S期，G2/M期的明显增多以及<italic>TGF-β1、Smad3、Collagen Ⅰ、Collagen Ⅲ、α-SMA</italic> mRNA及相应蛋白的表达明显增高，<italic>Smad7 </italic>mRNA及蛋白明显减少，差异有统计学意义。…”
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Induced Inflammatory and Oxidative Markers in Cerebral Microvasculature by Mentally Depressive Stress by Yuequan Zhu, Yazeed Haddad, Ho Jun Yun, Xiaokun Geng, Yuchuan Ding

“…NADPH oxidase (NOX) was activated by CRS (p<0.01), and mRNA and protein expressions of NOX subunits (gp91phox, p47phox, p67phox, and p22phox) in brain microvasculature were found to be increased. …”
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Angiogenesis Regulated by Electroacupuncture Combined with Rehabilitation Training in Cerebral Ischemia Involved in PI3K/Akt Signaling Pathway by Wenchao YAO, Mengxing LI, Qing LIU, Mingyue TONG, Peng HE, Wei TANG

“…The expression of protein in the combined acupuncture group was higher than that of the other two groups, and the difference was statistically significant respectively (<italic>P</italic>&lt; 0.01); the trend of each variable at different time points was significantly different respectively (<italic>F</italic>_Time=100.462, 53.714, 106.552, 75.381, <italic>P</italic>&lt; 0.001), the expression of VEGF and Akt protein in the model group increased on the 7th day, and the difference was statistically significant compared with 3 d and 14 d respectively (<italic>P</italic>&lt; 0.05); the expression of BDNF and PI3K increased on the 7th day in the model group, compared with that on the 3rd day, the difference was statistically significant (<italic>P</italic>&lt; 0.01); the expression of VEGF, BDNF, PI3K, Akt protein increased significantly on the 14th day of the combined acupuncture group, and the difference was statistically significant compared with 3 d and 7 d respectively (<italic>P</italic>&lt; 0.05); The expression group×time all had a significant interaction (<italic>P</italic>&lt; 0.001). (4) The analysis of variance of repeated measurements of VEGF, BDNF, PI3K, Akt mRNA in rat brain tissues in ischemic area showed that the variables were statistically significant in different groups respectively (<italic>F</italic>_Intergroup=445.845, 427.743, 918.388, 543.814, <italic>P</italic>&lt; 0.001), the combined acupuncture group expression was higher than the other two groups, the difference was statistically significant respectively (<italic>P</italic>&lt; 0.01); the change trend of each variable at different time points was significantly different (<italic>F</italic>_Time=100.343, 13.984, 9.162, 32.931, <italic>P</italic>&lt; 0.001), the expression of BDNF and PI3K mRNA in the model group increased on the 7th day, and the difference was statistically significant respectively (<italic>P</italic>&lt; 0.05, <italic>P</italic>&lt; 0.01); the expression of VEGF mRNA in the model group increased on the 7th day, compared with that on the 14th day, the difference was statistically significant (<italic>P</italic>&lt; 0.01); the expression of VEGF, BDNF, PI3K, Akt mRNA increased significantly on the 7th day in the combined acupuncture group, and the difference was statistically significant compared with 3 d and 14 d respectively (<italic>P</italic>&lt; 0.05, <italic>P</italic>&lt; 0.01); the mRNA expression group×time had significant interaction (<italic>P</italic>&lt; 0.001).Conclusion:Under the influence of intervention effect, time effect and the interaction of the two, the combination of electroacupuncture and rehabilitation training can play a protective role in cerebral ischemia, which may be related to the control of the expression of VEGF and BDNF and the downstream transduction pathway PI3K/Akt, which mediates the angiogenesis after cerebral ischemia.…”
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Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells Promote Trophoblast Cell Proliferation and Migration by Targeting TFPI2 in Preeclampsia by Ying Chen, Chenchen Zhou, Xiaobo Zhao, Ronghua Che, Yuelin Wu, Sheng Wan, Jinda Pei, Liping Yao, Xiaolin Hua

“…The messenger ribonucleic acid (mRNA) levels of miR-195 and tissue factor pathway inhibitor 2 (TFPI2) were measured in placental tissues derived from normal and preeclampsia patients by real-time polymerase chain reaction (PCR). …”
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TPX2 Serves as a Cancer Susceptibility Gene and Is Closely Associated with the Poor Prognosis of Endometrial Cancer by Jun Wang, Hua Zheng, Hui He, Shuying Meng, Yatian Han, Zhe Su, Hanbing Yan, Yanan Zhang

“…Detailed clinicopathological information was collected, and changes in the mRNA and protein levels of hub DEGs were analyzed in EC. …”
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和厚朴酚对人白血病细胞系U937细胞增殖和凋亡的影响

“…【目的】 探讨和厚朴酚(HNK)对白血病细胞系U937细胞增殖和凋亡的影响65377;【方法】 将U937细胞和正常外周血单核细胞分别与HNK共培养,Hoechst33342荧光染色检测细胞凋亡形态,用噻唑蓝比色法(MTT)检测HNK对细胞增殖的抑制作用;流式细胞仪检测凋亡;罗丹明123检测线粒体膜电位;Caspase3/7活性检测试剂盒检测Caspase3/7活性;荧光定量PCR(FQ-PCR)检测Caspase-365380;Caspase-7 mRNA水平变化65377;【结果】 HNK对U937的体外增殖有显著抑制作用,48 h半数抑制浓度(IC50)为11.8 μg/mL,而对正常外周血单核细胞的IC50为40.3 μg/mL,Annexin V/PI双标染色显示,细胞凋亡与和厚朴酚呈浓度和时间依赖相关65377;HNK明显降低U937细胞线粒体膜电位,增加Caspase3/7活性及mRNA表达水平,但对正常外周血单核细胞的增殖抑制和凋亡作用不明显65377;【结论】 HNK可能通过激活caspase-3/7抑制U937细胞增殖65380;诱导U937细胞凋亡65377;…”
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Effect of fecal microbiota transplantation on ulcerative colitis model in rats: The gut–brain axis by Mahdis Morshedbak, Kaveh Rahimi, Mohammad Reza Tabandeh

“…The study examined several indicators, such as TLR4, MYD88, and NF-κB mRNA expression, along with oxidative stress factors. …”
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Feasibility of Metatranscriptome Analysis from Infant Gut Microbiota: Adaptation to Solid Foods Results in Increased Activity of Firmicutes at Six Months by Floor Hugenholtz, Jarmo Ritari, Lotta Nylund, Mark Davids, Reetta Satokari, Willem M. de Vos

“…Specific attention is given to the impact of removing rRNA from the mRNA on the phylogenetic and transcriptional profiling and its analysis depth. …”
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Stress-Related Gene Expression in Liver Tissues from Laying Hens Housed in Conventional Cage and Cage-Free Systems in the Tropics by María Paula Herrera-Sánchez, Roy Rodríguez-Hernández, Iang Schroniltgen Rondón-Barragán

“…Liver tissues from Hy-Line Brown hens housed in conventional cage and cage-free egg production systems at 60 and 80 weeks of production were used. mRNA transcript levels were determined by qPCR using the relative quantification method and ACTB as the reference gene. …”
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LRP6 调控经典 Wnt 信号通路作用于阿霉素诱导的 心肌细胞损伤 by 梁莉莹

“…【结果】在细胞及动物水平上，成功建立阿霉素心肌病模型；Dox引起LRP6的mRNA和蛋白水平下降；敲低LRP6加重阿霉素引起的心肌细胞凋亡和线粒体损伤；Dox和沉默LRP6均下调β-catenin，激活β-catenin可以逆转阿霉素造成的心肌损伤。…”
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Effects of FTY720 on Lung Injury Induced by Hindlimb Ischemia Reperfusion in Rats by Liangrong Wang, Feifei Chen, Yafei Pan, Lina Lin, Xiangqing Xiong

“…Results. W/D, P/A, and mRNA expressions of S1PL, SphK1, and SphK2 were higher in group IR than in group SM, while these were decreased in both groups F5 and F10 as compared to IR (p<0.05). …”
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Prenatal maternal stress in rats alters the epigenetic and transcriptomic landscape of the maternal-fetal interface across four generations by Stephanie E. King, Nicola A. Schatz, Olena Babenko, Yaroslav Ilnytskyy, Igor Kovalchuk, Gerlinde A. S. Metz

“…The transgenerational persistence of critical DNAm, miRNA and mRNA signatures may explain familial non-genetic disease risks.…”
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The effect of KUS121, a novel VCP modulator, against ischemic injury in random pattern flaps. by Koichi Yoshimoto, Ryosuke Ikeguchi, Takashi Noguchi, Maki Ando, Daichi Sakamoto, Terunobu Iwai, Kohei Nishitani, Hanako Ohashi Ikeda, Akira Kakizuka, Shuichi Matsuda

“…Immunohistochemistry showed that the KUS121 treatment reduced the number of apoptotic cells in the distal third of the flap (p < 0.01); moreover, in the KUS121-treated rats, the number of cells expressing CHOP, an endoplasmic reticulum (ER) stress marker, in the middle third of the flap was significantly lower than in the controls (p < 0.01). We examined the mRNA expression of Ddit3 (CHOP) and Casp3 (caspase-3) on day one after the surgery; mRNA expression of both genes appeared to decrease in the KUS121 group, as compared with the control group, although differences between groups were not significant. …”
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Phenotypic screening in zebrafish larvae identifies promising cyanobacterial strains and pheophorbide a as insulin mimetics by Tiago Ribeiro, Mariana Reis, Vitor Vasconcelos, Ralph Urbatzka

“…These were further characterized by measuring glucose levels in whole larvae, the expression of glucose transporters (GLUT 1–3) using western blot, and the mRNA expression levels of the glut2, pepck, and insa genes using real-time qPCR. …”
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盐酸二甲双胍对非酒精性脂肪肝大鼠脂肪组织肿瘤坏死因子α基因表达的影响 by 江庆澜, 李瑜元, 曾峥, 周永健, 杜洪, 黄健, 何淼

“…[方法]以高脂饲料饲喂雄性Wistar大鼠12周诱发NAFLD,然后转以基础饲料饲养,并分别进行盐酸二甲双胍干预与蒸馏水灌喂对照,每组8只,为期4周.从大鼠脂肪组织提取总RNA并逆转录成为cDNA,通过real-time qPCR检测肿瘤坏死因子α（TNF-α）mRNA的相对水平,以t检验分析两组间数据的差异.[结果]干预组大鼠脂肪组织的TNF-α mRNA相对水平平均值（0.063±0.006）比对照组（0.126±0.015）下降,其差异显著（t=4.662,P=0.001）.…”
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ABT737通过激活JNK/c-Jun通路上调Bim诱导宫颈癌细胞凋亡

“…【方法】 用MTT法检测ABT-737对人宫颈癌Hela细胞的生长抑制作用;用流式细胞术检测细胞凋亡率;用Western blot方法检测JNK、phospho-JNK、c-Jun、phospho-c-Jun以及Bim蛋白的表达;用RT-PCR检测Bim在mRNA水平的变化;用JNK特异性抑制剂SP600125和siRNA瞬时转染抑制JNK及c-Jun的活性。 …”
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The zebrafish moonshine gene encodes transcriptional intermediary factor 1gamma, an essential regulator of hematopoiesis. by David G Ransom, Nathan Bahary, Knut Niss, David Traver, Caroline Burns, Nikolaus S Trede, Noelle Paffett-Lugassy, Walter J Saganic, C Anthoney Lim, Candace Hersey, Yi Zhou, Bruce A Barut, Shuo Lin, Paul D Kingsley, James Palis, Stuart H Orkin, Leonard I Zon

“…Three different mon mutant alleles each encode premature stop codons, and enforced expression of wild-type tif1gamma mRNA rescues embryonic hematopoiesis in homozygous mon mutants. …”
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