Zerumbone Attenuates the Severity of Acute Necrotizing Pancreatitis and Pancreatitis-Induced Hepatic Injury by Deng Wenhong, Yu Jia, Wang Weixing, Chen Xiaoyan, Chen Chen, Xu Sheng, Jin Hao

“…The expression of I-κBα and NF-κB protein was evaluated by western blot and immunohistochemistry assay while ICAM-1 and IL-1β mRNA were examined by RT-PCR. The results showed that AMY, sPLA2, ALT, and AST levels and histopathological assay of pancreatic and hepatic tissues were significantly reduced following administration of zerumbone. …”
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Applications of Recombinant Dna Technology in Gastrointestinal Medicine and Hepatology: Basic Paradigms of Molecular Cell Biology. Part B: Eukaryotic Gene Transcription and Post-Tr... by Gary E Wild, Patrizia Papalia, Mark J Ropeleski, Julio Faria, Alan BR Thomson

“…The production of each mRNA in human cells involves complex interactions of proteins (ie, trans-acting factors) with specific sequences on the DNA (ie, cis-acting elements). …”
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Peptidoglycan recognition protein PGRP-5 is involved in immune defence and neuro-behavioral disorders in zebrafish embryos. by Xi Li, Guanghua Xiong, Manni Luo, Siwan Mao, Ruiying Zhang, Ziwei Meng, Juan Li, Xinjun Liao

“…Thirdly, the mRNA levels of DrPGRP-5 were greatly elevated after 6 h of E. coli infection but reached its highest value at 24 h after M. luteus stimulation. …”
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The Use of Antisense-Mediated Inhibition to Delineate The Role of Inflammatory Agents in The Pathophysiology of Spinal Cord Injury by Damien D. Pearse, Francisco C. Pereira, Katina Chatzipanteli, Francisco W. Dalton Dietrich, Mary Bartlett Bunge

“…Hybridization of the oligonucleotide to the mRNA then interferes with the normal processing of that mRNA at the ribosome or targets the RNA duplex for cleavage by the RNA digestive enzyme, ribonuclease H, resulting in greatly reduced expression of the coded protein. …”
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Modulatory Impacts of Probiotic on the Synthesis of Claudins and Mucins in the Gastrointestinal Tract of Broiler Chicks by ESI Mohammed, M Al-Rasheed, MS Ahmed

“…ABSTRACT This study investigated the effect of 3 strains of probiotic bacteria on mRNA synthesis of some claudins (claudins1(CLD1), CLD3, and CLD5), and mucins (MUC5AC and MUC2) in broilers’ digestive systems. …”
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抵抗素对C2C12肌细胞株葡萄糖代谢的影响

“…【目的】 抵抗素(resistin)是一种与胰岛素抵抗(IR)密切相关的脂肪细胞因子65377;本研究通过观察resistin对C2C12肌细胞株葡萄糖代谢的影响,以探讨resistin在骨骼肌IR发生中的作用65377;【方法】构建含resistin基因的重组表达质粒pcDNA3.1-resistin,用于转染C2C12肌细胞株,使其表达resistin,用3H-2-脱氧葡萄糖进行葡萄糖摄取试验,用D-[14C(U)]葡萄糖进行葡萄糖氧化和糖原合成试验,RT-PCR检测肌细胞的葡萄糖转运蛋白4(GLUT4)mRMA65377; 【结果】 pcDNA3.1-resistin转染的C2C12肌细胞株可表达resistin;resistin使肌细胞胰岛素刺激的葡萄糖摄取率减少约30%,并使GLUT4mRNA 表达降低28%,而对葡萄糖氧化和糖原合成无显著影响65377; 【结论】 resistin可能通过抑制骨骼肌GLUT4mRNA的表达而降低葡萄糖摄取,但对葡萄糖氧化和糖原合成影响较小,提示resistin在骨骼肌IR发生中的作用有限65377;…”
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Spatiotemporal Pattern of Neuroinflammation After Impact-Acceleration Closed Head Injury in the Rat by Servan Rooker, Sebastian Jander, Jos Van Reempts, Guido Stoll, Philippe G. Jorens, Marcel Borgers, Jan Verlooy

“…We found an early upregulation of IL-1β, IL-6, and TNF-α mRNA between 1 h and 7 h after injury; the expression of iNOS mRNA only revealed a significant increase at 4 h. …”
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Carvedilol Confers Ferroptosis Resistance in HL-1 Cells by Upregulating GPX4, FTH1, and FTL1 and Inducing Metabolic Remodeling Under Hypoxia/Reoxygenation by Yi-Chin Li, Mei-Ling Cheng

“…Also, Carvedilol promoted ferroptosis-related genes, ferritin heavy chain 1 (<i>FTH1</i>) and ferritin light chain 1 (<i>FTL1</i>) mRNA levels, to reduce lipid peroxidation under both hypoxia and HR. …”
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Modulatory effects of rutin on the expression of cytochrome P450s and antioxidant enzymes in human hepatoma cells by Karakurt Sedar

“…Besides, rutin treatment significantly inhibited mRNA expression of CYP3A4 (73.2 %, p=0.0014). Also, CYP1A1, NQO1 and GSTP1 mRNA expressions were significantly increased 2.77-fold (p = 0.029), 4.85- fold (p = 0.0051) and 9.84-fold (p < 0.0001), respectively.…”
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Development and characterization of a double-crested cormorant hepatic cell line, DCH22, for chemical screening by Tasnia Sharin, Doug Crump, Jason M. O’Brien

“…Exposure to 3,3′,4,4′,5-pentachlorobiphenyl (PCB-126), benzo-a-pyrene, ß-napthoflavone and phenacetin induced CYP1A activity and mRNA expression in DCH22 3D spheroids. Induction of CYP3A activity and mRNA expression was observed following exposure to hexabromocyclododecane (HBCD), tris(1,3-dichloroisopropyl)phosphate, carbamazepine, and metyrapone. …”
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Poly(A)-DG: A deep-learning-based domain generalization method to identify cross-species Poly(A) signal without prior knowledge from target species. by Yumin Zheng, Haohan Wang, Yang Zhang, Xin Gao, Eric P Xing, Min Xu

“…In eukaryotes, polyadenylation (poly(A)) is an essential process during mRNA maturation. Identifying the cis-determinants of poly(A) signal (PAS) on the DNA sequence is the key to understand the mechanism of translation regulation and mRNA metabolism. …”
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瘦素及瘦素受体在胃癌组织和胃癌细胞株中的表达 by 赵晓龙

“…MGC803及MKN45均有瘦素及瘦素受体表达;47例胃癌组织石蜡切片标本检测有瘦素表达20例,有瘦素受体表达23例,两者共同表达17例;19例新鲜胃癌组织标本,有瘦素mRNA表达13例,瘦素受体mRNA表达12例,两者共同表达10例;与对照组相比,100 ng/mL的瘦素可使胃癌细胞株SGC7901? …”
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Potential Biomarkers of the Earliest Clinical Stages of Parkinson’s Disease by Anelya Kh. Alieva, Elena V. Filatova, Aleksey V. Karabanov, Sergey N. Illarioshkin, Petr A. Slominsky, Maria I. Shadrina

“…Based on these results, it can be suggested that the upregulation of the mRNA levels of ATP13A2, PARK7, and ZNF746 in untreated patients in the earliest clinical stages can also be observed in the preclinical stages of PD, and that these genes can be considered as potential biomarkers of the preclinical stage of PD.…”
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Targeting RNA splicing modulation: new perspectives for anticancer strategy? by Xuemei Lv, Xiaoyu Sun, Yang Gao, Xinyue Song, Xiaoyun Hu, Lang Gong, Li Han, Miao He, Minjie Wei

“…Abstract The excision of introns from pre-mRNA is a crucial process in the expression of the majority of genes. …”
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Activation of TLR4-Mediated NF𝜅B Signaling in Hemorrhagic Brain in Rats by Weiyu Teng, Lishu Wang, Weishuang Xue, Chao Guan

“…Compared with saline control, the TLR4 mRNA and protein significantly increased starting at 6 hours after ICH, peaked on the 3rd day after ICH, and then decreased but still maintained at a higher level on the 7th day after ICH (𝑃<.05). …”
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Lutein Leads to a Decrease of Factor D Secretion by Cultured Mature Human Adipocytes by Yuan Tian, Aize Kijlstra, Johan Renes, Martin Wabitsch, Carroll A. B. Webers, Tos T. J. M. Berendschot

“…Lutein significantly inhibited adipocyte FD mRNA expression and FD protein release into adipocyte culture supernatants. …”
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Expression and Secretion of CXCL-8 and CXCL-10 From <emph>Mycobacterium Bovis</emph> BCG-Infected Human Epithelial Cells: Role of IL-4

“…We found that, although <emph>M. bovis</emph> BCG induced gene expression of CXCL-8 and CXCL-10 in <emph>M. bovis</emph> BCG-infected human epithelial cells, CXCL-8 mRNA level was significantly reduced by IL-4, whereas no significant effect of IL-4 was observed on CXCL10 mRNA level. …”
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Effects of Amelogenin on Proliferation, Differentiation, and Mineralization of Rat Bone Marrow Mesenchymal Stem Cells In Vitro by Masanobu Izumikawa, Keijiro Hayashi, Mohammad Ali Akbor Polan, Jia Tang, Takashi Saito

“…The mRNA expression of osteocalcin and DMP-1 was also stimulated for 24 hours and 0.5 hours, respectively, in amelogenin addition group. …”
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The potential of supplementing compound organic trace elements at lower levels in Chinese yellow- feathered broiler diets, part II: Impacts on growth performance, gut health, intes... by Xiaoyan Nie, Fei Zhao, Yucheng Yin, Qi Lu, Yang Dai, Rui Wang, Yiwen Ji, Huihua Zhang, Cui Zhu

“…Spearman correlation analysis showed that the mRNA of occludin and jejunal V/C ratio were positively correlated with the abundance of Firmicutes (phylum), but negatively correlated with the abundance of Bacteroidota (phylum). …”
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To Explore the Pathogenesis of Vascular Lesion of Type 2 Diabetes Mellitus Based on the PI3K/Akt Signaling Pathway by Jia-Rong Gao, Xiu-Juan Qin, Zhao-Hui Fang, Li-Shan, Li-Ping Han, Hui-Jiang, Ming-Fei Guo, Nan-Nan Jiang

“…RT-QPCR was used to detect the mRNA expression of endothelial markers PTEN, PI3K, Akt, and VEGF. …”
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