Dissecting the role of single nucleotide polymorphism of lymphotoxin beta gene during pig domestication using bioinformatic and experimental approaches by R. B. Aitnazarov, E. V. Ignatieva, N. E. Bazarova, V. G. Levitsky, S. P. Knyazev, Y. Gon, N. S. Yudin

“…The frequency of the GG genotype in the wild boar sample was significantly lower than in the pooled sample of domestic pigs. The LTB mRNA expression rate in the lymph node of minipigs with genotype GG tended to increase (p < 0.06) in comparison with carriers o allele A. …”
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Advanced Glycation End Products Enhance Murine Monocyte Proliferation in Bone Marrow and Prime Them into an Inflammatory Phenotype through MAPK Signaling by Xian Jin, Liang Liu, Yaping Zhang, Yin Xiang, Guizhi Yin, Yi Lu, Ludong Shi, Jian Dong, Chengxing Shen

“…In vitro, when the monocytes were treated with different dosages of AGEs (50, 150, and 300 μg/mL), we found that proliferation (CCK8) but not apoptosis (FCM) of the monocytes was induced; the mRNA expressions of proinflammatory cytokines (IL-1β and TNF-α) and GM-CSF were upregulated in a dose-dependent manner while mRNA levels of IL-10 and M-CSF were changed much less in monocytes (real-time PCR). …”
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Effects of Dietary Fish Meal Replaced by Fish Steak Meal on Growth Performance, Antioxidant Capacity, Intestinal Health and Microflora, Inflammatory Response, and Protein Metabolis... by Dianguang Zhang, Yunzong Zheng, Xuexi Wang, Dejuan Wang, Hongjie Luo, Wenbo Zhu, Weini Zhang, Zhengbang Chen, Jianchun Shao

“…The FSM75 and FSM100 diets significantly decreased enzyme activities of amylase (AMS), lipase (LPS), trypsin, catalase (CAT), and total superoxide dismutase (T-SOD) and the total antioxidant capacity (T-AOC), and increased the malondialdehyde (MDA) content in the liver of large yellow croaker. The mRNA expression levels of intestinal barrier and inflammatory response-related genes suggested that the FSM50, FSM75, and FSM100 diets significantly decreased the mRNA abundances of intestinal barrier-related genes and anti-inflammatory response-related genes, and increased the mRNA abundances of proinflammatory gene il-6 in the anterior intestine. …”
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Influence of luteolin on the apoptosis of esophageal cancer Eca109 cells and its mechanism of action by Shaokang Wang, Lingmeng Fu, Yi Wu, Hongmei Xiao, Jing Wang, Guiju Sun

“…Expression of caspase9 and caspase3 mRNA and protein was analyzed by real-time PCR and Western blot respectively. …”
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Effects of dietary dihydroartemisinin on growth performance, meat quality, and antioxidant capacity in broiler chickens by Xiaoxiao Pu, Yuxuan Liang, Jiafang Lian, Miaoxuan Xu, Yalan Yong, Hao Zhang, Lili Zhang, Jingfei Zhang

“…Dihydroartemisinin quadratically increased the hepatic mRNA expression of Nrf2. Compared to the control group, dihydroartemisinin at 10 and 20 mg/kg quadratically induced the hepatic mRNA expression of HO-1. …”
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Curcumin ameliorates oxaliplatin-induced allodynia response and melanocortin downregulation in the spinal cord by Alma Nuril Aliya, Luke Wongso, Diah Ayu Wakita Trimanda, Risda Maulida, I Nengah Budi Sumartha, Samirah Samirah, Abdulloh Machin, Long Chiau Ming, Abdelhakim Bouyahya, Mahardian Rahmadi, Junaidi Khotib, Сhrismawan Ardianto

“…Melanocortin 4 receptor (Mc4r), Pro-opiomelanocortin (Pomc) and Trpa1 mRNA expressions were measured using RT-qPCR. …”
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Adaptability of clams to prolonged heatwaves: Influences on gene expression, antioxidant capacity, and histology of Chinese cyclina Cyclina sinensis by Meimei Liu, Zhichao Wang, Hongwei Ni, Wenqing Zhuo, Gaoyuan Yuan, Zhiguo Dong

“…Under prolonged heatwave stress challenge, HSP27, HSP70B2 and HSP90β mRNA in hepatopancreas and mantle were up-regulated in short heatwave group. …”
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Th1/Th2 Dichotomy in Obese Women with Gestational Diabetes and Their Macrosomic Babies by A. Seck, A. Hichami, S. Doucouré, F. Diallo Agne, H. Bassène, A. Ba, C. Sokhna, N. A. Khan, A. Samb

“…T lymphocytes were purified from the total blood with Ficoll-Paque. The mRNA expression of inflammatory markers in T cells was determined by RT-qPCR. …”
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Identification of Crucial lncRNAs for Luminal A Breast Cancer through RNA Sequencing by Xinjian Jia, Hai Lei, Xuemei Jiang, Ying Yi, Xue Luo, Junyan Li, Yu Chen, Sha Liu, Chengcheng Yang

“…Four lncRNA-nearby and coexpressed mRNA pairs of HOXC-AS3-HOXC10, AC020907.2-FXYD1, AC026461.1-MT1X, and AC132217.1-IGF2 were identified. …”
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ELAVL1‐dependent SOAT2 exacerbated the pancreatitis‐like cellular injury of AR42J cells induced by hyperstimulation with caerulein by Yu‐Jing Sun, Hua‐Ying Chen, Xiao‐Qin Lai

“…Moreover, ELAVL1 stabilized SOAT2 mRNA expression in AR42J cells. SOAT2 overexpression attenuated the effects induced by ELAVL1 silencing in caerulein‐exposed AR42J cells. …”
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Effects of Glycyrrhizin (GL) Supplementation on Survival, Growth Performance, Expression of Feeding-Related Genes, Activities of Digestive Enzymes, Antioxidant Capacity, and Expres... by Yuntao Wang, Wenxuan Xu, Jianmin Zhang, Jiahui Liu, Zhen Wang, Yongtao Liu, Kangsen Mai, Qinghui Ai

“…Compared with the control, the mRNA expression of orexigenic factor genes including neuropeptide Y (npy) and agouti-related protein (agrp) were significantly increased in larvae fed the diet with 0.005% GL, while the mRNA expression of anorexigenic factor genes including thyrotropin-releasing hormone (trh), cocaine and amphetamine regulated transcript (cart), and leptin receptor (lepr) were significantly decreased in larvae fed the diet with 0.005% GL (P<0.05). …”
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The expression profile of genes associated with behavior, stress, and adult neurogenesis along the hippocampal dorsoventral axis in tame and aggressive foxes by Yu. V. Alexandrovich, E. V. Antonov, S. G. Shikhevich, A. V. Kharlamova, L. V. Meister, Y. V. Makovka, D. V. Shepeleva, R. G. Gulevich, Yu. E. Herbeck

“…In tame foxes, significantly elevated mRNA levels were recorded for several genes: CYP26B1 for cytochrome P450 26B1 and ADRA1A for α1A adrenergic receptor in the dorsal hippocampus, whereas the level of NR3C2 mRNA for mineralocorticoid receptor was higher in the ventral. …”
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Oyster Mushroom (Pleurotus ostreatus) Ethanolic Extract Inhibits Pparg Expression While Maintaining the Methylation of the Pparg Promoter During 3T3-L1 Adipocyte Differentiation by Ariyanto EF, Farahana AK, Sudirman GSJ, Widiarsih E, Qomarilla N, Rahayu NS, Wikayani TP, Heryaman H, Wira DW, Triatin RD, Bashari MH, Pamela Y, Pratiwi YS, Ghozali M

“…The extract was obtained from 80 g of dried oyster mushroom powder extracted three times with 800 mL of ethanol, filtered, evaporated, and reconstituted in dimethyl sulfoxide (DMSO) to final concentrations of 0, 25, 50, and 100 μg/mL, with DMSO limited to 0.5% in all solutions. Pparg mRNA expression was quantified by qRT-PCR analysis and Pparg promoter methylation levels were measured quantitatively by pyrosequencing of bisulfite-treated DNA samples.Results: The addition of 25 μg/mL oyster mushroom ethanolic extract significantly suppressed Pparg mRNA expression with no significant change in the Pparg promoter methylation levels.Conclusion: Oyster mushroom ethanolic extract inhibited Pparg mRNA expression without altering Pparg promoter methylation, suggesting reduced adipocyte differentiation. …”
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Epigenetic alterations of 11beta-hydroxysteroid dehydrogenase 1 gene in the adipose tissue of patients with primary aldosteronism by Yoshimichi Takeda, Masashi Demura, Mitsuhiro Kometani, Shigehiro Karashima, Daisuke Aono, Seigo Konishi, Shin-ichi Horike, Makiko Meguro-Horike, Takashi Yoneda, Yoshiyu Takeda

“…PA, SCS, and Cu were diagnosed according to the guideline of the Japan Endocrine Society. The mRNA level of HSD11B1 was quantified using real-time PCR. …”
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Exploring precision therapeutics: computational design of antisense oligonucleotides targeting AXL gene transcripts in multiple sclerosis treatment management by Bhargav Shreevatsa, Bhargav Shreevatsa, Abhigna Nagaraj, Chandan Dharmashekar, Anisha Jain, Bhavana Harendra, Siddesh V. Siddalingegowda, Haneen A. Al-Mazroua, Sheikh F. Ahmad, Shashanka K. Prasad, Chandrashekar Srinivasa, Chandan Shivamallu, Shiva Prasad Kollur

“…The ASOs were optimized with a focus on stability, binding affinity, and specificity towards AXL mRNA while minimizing off-target effects. To investigate ASO-mRNA interactions and gauge their ability to alter AXL expression, Molecular Docking was performed. …”
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甘露糖对巨噬细胞炎症反应的双向调节作用 by 吴惠娟, 韦曲星, 张盛昔, 胡因铭, 王乐旬

“…和LPS处理相比，LPS + 甘露糖2 mmol/L（Man2）能够增高IL-1β、IL-12和TNF-α的mRNA水平以及蛋白水平（均<italic>P</italic>&lt;0.05）；LPS + Man20显著抑制IL-1β、IL-12、TNF-α、IL-6和CCL2的mRNA水平和蛋白水平（均<italic>P</italic>&lt;0.05）。…”
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RNA m6A-mediated post-transcriptional repression of glucocorticoid receptor in LPS-activated Kupffer cells on broilers by Yulan Zhao, Yidan Jiang, Yue Feng, Ruqian Zhao

“…We observed that YTHDF2 siRNA can alleviate LPS-induced GR mRNA stability decrease. Subsequently, specific m6A sites on GR were predicted and verified. …”
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Different Activity of the Biological Axis VEGF-Flt-1 (fms-Like Tyrosine Kinase 1) and CXC Chemokines between Pulmonary Sarcoidosis and Idiopathic Pulmonary Fibrosis: A Bronchoalveo... by Katerina M. Antoniou, Giannoula Soufla, Athanasia Proklou, George Margaritopoulos, Christiana Choulaki, Rena Lymbouridou, Katerina D. Samara, Demetrios A. Spandidos, Nikolaos M. Siafakas

“…A statistically significant increase has been detected in VEGF mRNA expression in IPF in comparison with pulmonary sarcoidosis (𝑃=.03). …”
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Networks of pre-diagnostic circulating RNA in testicular germ cell tumour by Joshua Burton, Trine B. Rounge, Trine B. Haugen, Marcin W. Wojewodzic

“…In this study, we performed Weighted Gene Co-expression Network Analysis (WGCNA) on mRNA and miRNA data from these samples. Central genes (hub genes) enriched functional pathways, and regulatory feature prediction were identified for all TGCT subtypes together and according to histology. …”
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The Efficacy of Jing Wan Hong Ointment for Nerve Injury Diabetic Foot Ulcer and Its Mechanisms by Shumei Jin, Mixia Zhang, Yan Gao, Xuebin Zhang, Guangzhi Cui, Yanjun Zhang

“…Moreover, the mechanisms of this effect seem to be dependent on increased expression of PDGF mRNA, but there was no influence on the expression of TGF-β, VEGF, and FLT-1 mRNA.…”
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