ERK1/2 和 P38 信号通路在瘦素诱导巨噬细胞TNF- α表达中的作用

“…ERK1/2 的特异性抑制剂 PD98059、U0126 和 p38 的特异抑制剂 SB203580 能够分别抑制瘦素引起的 ERK1/2、p38 蛋白磷酸化以及 TNF- αmRNA 增加, 两者的联合作用可以强烈抑制 TNF- αmRNA 的表达, 而 JNK 信号转导途径与瘦素诱导 PM表达 TNF- α无关。…”
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Upregulated GSDMB in Clear Cell Renal Cell Carcinoma Is Associated with Immune Infiltrates and Poor Prognosis by Yuanshan Cui, Zhongbao Zhou, Yumeng Chai, Yong Zhang

“…Correlation analysis showed that the mRNA expression of GSDMB was associated with immune infiltrates and the purity of the tumor. …”
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心肺复苏大鼠脑基质金属蛋白酶9的表达和血脑屏障的变化

“…【目的】 探讨心肺复苏后早期大鼠脑组织基质金属蛋白酶9(MMP9)的表达和血脑屏障的变化规律65377;【方法】 将80只大鼠随机分为假手术组(n = 40)和复苏组(n = 40)65377;假手术组仅进行麻醉和气管插管65380;血管穿刺,不进行窒息,复苏组用气管夹闭窒息法致使心跳骤停后进行复苏,其他与对照组相同,分别在065380;365380;965380;24 h和48 h 5个时间点处死大鼠后,取样,测定脑组织MMP9的表达65380;MMP9 mRNA65380;脑含水量与脑组织伊文思蓝含量的变化,电镜观察脑组织的超微结构65377;【结果】 复苏组心肺复苏后365380;965380;24和48 h脑组织MMP9表达明显高于假手术组,MMP9 mRNA含量与假手术组相比差异有统计学意义,脑含水量与假手术组比较明显升高,与假手术组比较,脑组织伊文思蓝含量明显升高;超微结构发生改变65377; 【结论】 在心肺复苏大鼠脑缺血模型中MMP9蛋白和MMP9mRNA的表达明显升高;脑组织的脑含水量和伊文思蓝含量明显升高,血脑屏障受到破坏;电镜超微结构观察证实脑组织损伤明显;这些变化在心肺复苏后24 h达高峰65377;…”
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Downregulation of the Proinflammatory State of Circulating Mononuclear Cells by Short-Term Treatment with Pioglitazone in Patients with Type 2 Diabetes Mellitus and Coronary Artery... by Andreas Pfützner, Alexander Weise, Elisabeth Pfützner-Riehn, Georg Lübben, Michael Morcos, Efstrathios Karagiannis, Matthias Weber, Thomas Forst

“…This study was performed to investigate the influence of a short-term treatment with pioglitazone versus placebo on inflammatory activation of mononuclear cells (mRNA expression/protein secretion of inflammatory markers). …”
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AMPK Activation in TET2 Downregulated Leukemia Cells Upon Glutamine Limitation by Ahsen Merve BAYRAK, Burcu YUCEL

“…In glutamine-deficient medium, the mRNA expression of glutamine synthetase is increased, which could be related to glutamine addiction in cells. …”
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Therapeutic Effect of Gypenosides on Antioxidant Stress Injury in Orbital Fibroblasts of Graves’ Orbitopathy by Chao Ma, Haoyu Li, Wei Liu, Shuwen Lu, Xian Li, Jinyuan Chen, Kaijun Li, Wenzhan Wang

“…The expression of apoptosis-related protein mRNA was analyzed by RT-qPCR technique. ROS and SOD test suites were employed to detect the oxidative stress level in OFs. …”
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Short-Chain Fatty Acids Promote Immunotherapy by Modulating Immune Regulatory Property in B Cells by Cai-Jie Zhou, Bai-Ling Xie, Hai-Yang Han, Yin Wang, Yong-Hua Wang, Jing-Yi Hong, Yi-Xia Wei, Zhi-Gang Liu, Yan Feng, Gui Yang, Ping-Chang Yang

“…The spontaneous IL-10 mRNA decay was observed in B cells, which was prevented by the presence of PA through activating GPR43. …”
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Computational analysis of spliced leader trans-splicing in the regenerative flatworm <i>Macrostomum lignano</i> reveals its prevalence in conserved and stem cell related genes by K. V. Ustyantsev, E. V. Berezikov

“…One of the functions of SL trans-splicing is processing polycistronic pre-mRNA molecules transcribed from operons, when several genes are transcribed as one pre-mRNA molecule. …”
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TDP-43 nuclear retention is antagonized by hypo-phosphorylation of its C-terminus in the cytoplasm by Célia Rabhi, Nicolas Babault, Céline Martin, Bénédicte Desforges, Alexandre Maucuer, Vandana Joshi, Serhii Pankivskyi, Yitian Feng, Guillaume Bollot, Revital Rattenbach, David Pastré, Ahmed Bouhss

“…We show that C-terminus phosphorylation prevents the recruitment of TDP-43 in mRNA-rich stress granules only under acute stress conditions because of a low affinity for mRNA but not under mild stress conditions. …”
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Chronic Glucocorticoid-Rich Milieu and Liver Dysfunction by Hernán Gonzalo Villagarcía, Vanesa Sabugo, María Cecilia Castro, Guillermo Schinella, Daniel Castrogiovanni, Eduardo Spinedi, María Laura Massa, Flavio Francini

“…MSG rats displayed degenerated optic nerves, hypophagia, low body and liver weights, and enlarged adipose tissue mass; higher peripheral levels of glucose, triglycerides, insulin, uric acid, leptin, corticosterone, transaminases and TBARS, and peripheral and liver insulin resistance; elevated liver OS, inflammation markers, and glucokinase (mRNA/activity) and fructokinase (mRNA). Additionally, MSG liver phosphofructokinase-2, glucose-6-phosphatase (mRNA and activity) and glucose-6-phosphate dehydrogenase, Chrebp, Srebp1c, fatty acid synthase, and glycerol-3-phosphate (mRNAs) were increased. …”
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A Novel Role of Globular Adiponectin in Treatment with HFD/STZ Induced T2DM Combined with NAFLD Rats by Hong Ma, Guo-Ping You, Xuan-Pu Zhang, Xiang-Jiu Yang, Hua-Dong Lu, Yan-Ling Huang, Wen-Qiang Zhang

“…But the protein and mRNA expressions of adipoR1 increased (P<0.05, resp.) in T2DM group and no change was found in the gAd-treated group. …”
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Macrophage Migration Inhibitory Factor Interacting with Th17 Cells May Be Involved in the Pathogenesis of Autoimmune Damage in Hashimoto’s Thyroiditis by Haibo Xue, Yuhua Yang, Ying Zhang, Shoujun Song, Li Zhang, Lei Ma, Tingting Yang, Huan Liu

“…Results. MIF mRNA, IL-17A mRNA expressions and Th17 cells percentages, serum MIF, and IL-17A protein levels were all significantly higher in HT patients, even in euthyroid subgroup. …”
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Genetic Polymorphism and Expression of CXCR4 in Breast Cancer by Marina Okuyama Kishima, Karen Brajão de Oliveira, Carolina Batista Ariza, Carlos Eduardo Coral de Oliveira, Roberta Losi Guembarovski, Bruna Karina Banin Hirata, Felipe Campos de Almeida, Glauco Akelinghton Freire Vitiello, Kleber Paiva Trugilo, Alda Fiorina Maria Losi Guembarovski, Walter Jorge Sobrinho, Clodoaldo Zago Campos, Maria Angelica Ehara Watanabe

“…The present study aimed to investigate the influence of CXCR4 rs2228014 polymorphism on its mRNA and protein expression in breast cancer samples. …”
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Modulation of DAPK1 expression by its alternative splice variant DAPK1-215 in cancer by QingShui Wang, ShuYun Weng, WenTing Zhong, YouYu Lin, Yan Yu, YiMin Huang, LiLin Ge, XiuLi Zhang, FangQin Xue, Yehuda G. Assaraf, Yao Lin

“…Finally, clinical liver cancer specimens were analyzed to assess the prognostic relevance of DAPK1-215 and DAPK1 mRNA levels. Results DAPK1-215 downregulated DAPK1 expression in liver, kidney, and gastric cancer cells by reducing DAPK1 mRNA stability. …”
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The Suppression of Signal Transducer and Activator of Transcription-3 in A549&nbsp;human Lung Carcinoma Cells Induced by Marine Sponge Callyspongia aerizusa by Hadisaputri YE, Nurhaniefah AA, Mutakin M, Hendriani R, Rezano A, Sopyan I, Yusnaini Y, Asikin Y, Abdulah R

“…Moreover, IL-6 and HIF-1α mRNA expression levels decreased during 24 hours, while VEGF mRNA had a slight decrease compared to untreated cells.Conclusion: In conclusion, the ergosteroids present in marine sponge C. aerizusa extract signified a remarkable reduction in malignancy, migration, and invasion capabilities in A549 lung carcinoma cells. …”
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Level of CYP4G19 Expression Is Associated with Pyrethroid Resistance in Blattella germanica by Guang-zhou Guo, Yi-jie Geng, Da-na Huang, Cai-fang Xue, Ren-li Zhang

“…Two to eight days after injection, the result showed that CYP4G19 mRNA expressions were significantly reduced in the groups injected with dsRNAs.…”
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Competing Endogenous RNA: The Key to Posttranscriptional Regulation by Rituparno Sen, Suman Ghosal, Shaoli Das, Subrata Balti, Jayprokas Chakrabarti

“…It has recently been discovered that, apart from protein-coding ceRNAs, pseudogenes, long noncoding RNAs (lncRNAs), and circular RNAs act as miRNA “sponges” by sharing common MRE, inhibiting normal miRNA targeting activity on mRNA. These MRE sharing elements form the posttranscriptional ceRNA network to regulate mRNA expression. ceRNAs are widely implicated in many biological processes. …”
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Modulation of Nrf2 expression by targeting i-motif DNA by E. F. Warner, D. Guneri, M. A. O’Connell, C. J. MacDonald, Z. A. E. Waller

“…We identified small molecule ligands that interact with this i-motif structure and one significantly upregulated Nrf2 mRNA expression, and one ligand reduced Nrf2 mRNA expression in human cancer cells. …”
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Calculation of Free Energy Consumption in Gene Transcription with Complex Promoter Structure by Lifang Huang, Peijiang Liu, Kunwen Wen, Jianshe Yu

“…Then, we show that the numbers of the ON and OFF states of a promoter can reduce energy consumption of the gene system and the Fano factor of mRNA, and in contrast to other regulatory ways, the cooperative binding of transcription factors to DNA sites always reduces energy consumption but amplifies the mRNA noise. …”
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The effect of COVID-19 and COVID-19 vaccines on chronic spontaneous urticaria: single center experience by Öner Özdemir, Ümmügülsüm Dikici

“…As a result, omalizumab had to be started in 3/8 patients after mRNA vaccination due to CSU exacerbation or treatment failure. …”
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