microRNA-21对膀胱癌T24细胞增殖的影响 及对VEGF和AP1蛋白的调节

“…【目的】 研究microRNA-21(miR-21)对膀胱癌T24细胞系中血管内皮生长因子(vascular endothelial growth factor, VEGF)和活化因子蛋白-1(activator protein-1, AP1)及mRNA表达的调节作用,探讨miR-21对膀胱癌T24细胞增殖的影响65377; 【方法】 用人工合成的miR-21相应的双链互补DNA片段,插入pSilencerTM 3.1-H1 neo载体表达载体,经测序鉴定后作为microRNA的表达质粒;用脂质体将miR-21高表达质粒转染进T24膀胱癌细胞,经G418筛选获得阳性克隆65377;用RT-PCR技术和Western blot方法分别检测该细胞系中VEGF和AP1的mRNA和蛋白表达情况65377;四甲基偶氮唑蓝(MTT)法检测膀胱癌T24细胞增殖的变化情况65377;【结果】 Western blot结果显示miR-21能够增加VEGF和AP1蛋白的表达,RT-PCR方法分析显示VEGF和AP1 mRNA水平升高65377;MTT显示转染后细胞的生长速率比未转染的细胞明显加快65377;【结论】 miR-21促进T24膀胱癌细胞增殖, 提示可能与VEGF和AP1 的低表达有关65377;…”
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In Situ Cytokine Expression and Morphometric Evaluation of Total Collagen and Collagens Type I and Type III in Keloid Scars by Isabela Rios da Silva, Luciana Colombo Rodrigues da Cunha Tiveron, Marcos Vinicius da Silva, Alberto Borges Peixoto, Carla Aparecida Xavier Carneiro, M. A. dos Reis, Pedro Carvalho Furtado, Bárbara Rocha Rodrigues, Virmondes Rodrigues, Denise Bertulucci Rocha Rodrigues

“…Significantly, the expression of mRNA for TGF-β in keloid was increased, the expressions of IFN-γ, IFN-γR1, and IL-10 were lower, and IFN-γR1 and TNF-α had no statistical difference. …”
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Natalizumab Treatment Modulates Peroxisome Proliferator-Activated Receptors Expression in Women with Multiple Sclerosis by Véronique Ferret-Sena, Alexandra Maia e Silva, Armando Sena, Inês Cavaleiro, José Vale, Bruno Derudas, Giulia Chinetti-Gbaguidi, Bart Staels

“…At three months, NTZ increased PPARβ/δ mRNA (p=0.009) in comparison to baseline, while mRNA expression of PPARγ and CD36 (a well-known PPAR target gene) was lower in comparison to healthy controls (p=0.026 and p=0.028, resp.). …”
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Effect of vasopressin on the expression of genes for key enzymes of interstitial hyaluronan turnover and concentration ability in WAG rat kidneys by L. N. Ivanova, A. V. Babina, G. S. Baturina, L. E. Katkova

“…The V2 receptor-selective vasopressin analog dDAVP (100 μg/kg bw, ip, twice a day for 2 days) induced a considerable decrease in Has2 mRNA content in the papilla with less pronounced changes in the cortex. …”
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Do Salivary <i>Cullin7</i> Gene Expression and Protein Levels Provide Advantages over Plasma Levels in Diagnosing Breast Cancer? by Ceren Tilgen Yasasever, Derya Duranyıldız, Süleyman Bademler, Hilal Oğuz Soydinç

“…Considering the possibility of Cul7 being a biomarker at the protein and mRNA levels, plasma is thought to be a better study material for Cul7. …”
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Base Composition Characteristics of Mammalian miRNAs by Bin Wang

“…MicroRNAs (miRNAs) are short RNA sequences that repress protein synthesis by either inhibiting the translation of messenger RNA (mRNA) or increasing mRNA degradation. Endogenous miRNAs have been found in various organisms, including animals, plants, and viruses. …”
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Sequences of acetyl CoA carboxylase promoter for tumour necrosis factor action by Keerang Park, Michael E. Pape, Ki-Han Kim

“…Tumour necrosis factor (TNF) inhibits the accumulation of acetyl CoA carboxylase (ACC) mRNA by decreasing the rate of ACC gene transcription. …”
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Mechanism of Bushen Zhuangjin Decoction to Promote BMSCs Homing and Protect Articular Cartilage in Mice by the SDF-1/CXCR4 Axis by HUANG Yanfeng, MA Dezun, FU Changlong, YE Jinxia, HUANG Yunmei, LI Xihai

“…The fluorescence intensity of SDF-1 in bone tissue was observed by laser confocal microscopy. qPCR and Western blot were used to detect mRNA transcription level and protein relative expression level of homing-related regulatory factors in each group. (2) In the cell experiment, 4-week-old SPF male C57BL/6 mice were selected and the primary BMSCs were extracted by the whole bone marrow adherence method. …”
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Effects of Exercise Preconditioning Mediating Oxidative Stress to Regulate PI3K/Akt and Nrf2/HO-1 Pathways to Alleviate Cognitive and Learning Disorders in Rats by KONG Haijun, LI Xinlong, WANG Fenghua, CHEN Xiaoan

“…The number of Nissl bodies in CA1 region decreased (<italic>P</italic>&lt;0.05). The mRNA expression levels of PI3K, Nox1 and Duox2 increased (<italic>P</italic>&lt;0.05), while the mRNA expression level of Akt decreased (<italic>P</italic>&lt;0.05). …”
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Effect of Tiaoxin Recipe on Memory Learning and Synaptic Plasticity in APP/PS1 Double Transgenic Alzheimer's Disease Mice based on αCaMKⅡ-CREB-BDNF Signal Pathway by Xiaowen WANG, Hong QIAN, Chunjuan SHEN, Yanjun LI, Haixin YUAN, Jian WANG

“…Objective:To observe the effects of Tiaoxin recipe on long-term potentiation (LTP) in hippocampal CA1 region, hippocampal alpha calcium/calmodulin-dependent kinaseⅡ (αCaMKⅡ), cyclic adenosine monophosphate response element binding protein (CREB), brain derived neurotrophic factor (BDNF), postsynaptic density 95 (PSD-95) mRNA and protein expression in hippocampal CA1 region of APP/PS1 double transgenic mice. …”
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Effect and Mechanism of Electroacupuncture at the Affected Side of Quchi （LI 11） and Yanglingquan （GB 34） Acupoints in Rats with Limb Spasms after Cerebral Infarction by ZHAN Sheng, HUANG Linxing, YI Lizhen, CHEN Ruixue, HUANG Huiyuan, YUE Zenghui

“…RT-PCR was used to analyze mRNA transcription levels of RFK and NOX2 mRNA.Results(1) Zea-Longa neurological deficit score and MAS score: compared with that before treatment, Zea-Longa neurological deficit score and MAS score of the electroacupuncture group decreased significantly after treatment (<italic>P</italic>&lt;0.05). …”
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卵巢子宫内膜异位囊肿患者免疫调节细胞Th17/Treg 失衡的 临床研究 by 杨娟, 韦成厚, 祝彩霞, 耿慧珍, 邓松清, 牛刚

“…③卵巢子宫内膜异位囊肿患者异位内膜Treg特异转录因子FoxP3 mRNA表达水平较两组在位内膜 均明显升高，Th17特异转录因子ROR-γt mRNA表达水平较两组在位内膜均明显降低。…”
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NADPH氧化酶在TGF-β1诱导大鼠小管上皮细胞 表达炎症因子中的作用 by 张海燕, 姜宗培, 常洁, 李晓艳, 朱恒梅, 余学清

“…p47phox和 p67phox亚单位以及MCP-1和IL-6 mRNA的表达采用RT-PCR检测?细胞上清液中MCP-1的含量采用ELISA检测?【结果】 TGF-β1可显著上调NADPH氧化酶p67phox亚单位mRNA的表达, 8 h为对照的2.43倍 (P < 0.01),24 h达3.59倍(P < 0.01)?…”
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Artificially Cultivated Ophiocordyceps sinensis Alleviates Diabetic Nephropathy and Its Podocyte Injury via Inhibiting P2X7R Expression and NLRP3 Inflammasome Activation by Chao Wang, Xiao-xia Hou, Hong-liang Rui, Li-jing Li, Jing Zhao, Min Yang, Li-jun Sun, Hong-rui Dong, Hong Cheng, Yi-Pu Chen

“…The experiments in vivo and in vitro both displayed that the mRNA and protein expression of P2X7R, NLRP3, ASC, caspase1 (procaspase-1 mRNA in the gene level and active caspase-1 subunit P10 in the protein level), IL-1β, and IL-18 was significantly upregulated and the mRNA and protein expression of podocyte-associated molecules was significantly changed (downregulation of nephrin, podocin, and WT-1 expression and upregulation of desmin expression) indicating podocyte injury in the kidney tissue of DN rats and in the HG-stressed mouse podocytes, respectively. …”
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DNA去甲基化酶TET在神经母细胞瘤患者中表达及意义 by 杨洋, 杨风雨, 张晓燕, 黄玠烨, 周倜, 齐炜炜, 邹焱, 杨霞

“…TET1表达与肾上腺素能受体2（β2-AR）表达呈正相关（<italic>r</italic>=0.347，<italic>P</italic>=0.023）。②生物信息学分析结果表明，TET3 mRNA表达水平与ADNP（神经保护蛋白）、ARID2（染色质重塑因子）等抑癌基因的mRNA表达水平呈正相关，与CD63（四次跨膜蛋白）、JOSD2（去泛素化酶）等癌基因的mRNA表达水平呈负相关；TARGET数据库中TET3 mRNA水平与神经母细胞瘤患者总生存期（OS）和无事件生存期（EFS）呈正相关（<italic>P</italic>=0.024， <italic>P</italic>=0.014）。…”
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Fission yeast essential nuclear pore protein Nup211 regulates the expression of genes involved in cytokinesis. by Domenick Kamel, Ayisha Sookdeo, Ayana Ikenouchi, Hualin Zhong

“…The fission yeast basket nucleoporin Nup211 is implicated in mRNA export and is essential for cell viability. …”
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A review of COVID-19 vaccination and the reported cardiac manifestations by Jamie Sin Ying Ho, Ching-Hui Sia, Jinghao Nicholas Ngiam, Poay Huan Loh, Nicholas Wen Sheng Chew, William Kok-Fai Kong, Kian-Keong Poh

“…In Singapore, 9.03 million doses of the mRNA COVID-19 vaccines by Pfizer-BioNTech and Moderna have been administered, and 4.46 million people are fully vaccinated. …”
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充血性心力衰竭犬心肌诱生型一氧化氮合酶的表达

“…【方法】建立快速心 脏起搏致心力衰竭的动物模型, 用RT-PCR 方法观察心力衰竭不同阶段心肌组织iNOS mRNA 的表达。【结果】在快速心脏起 搏致心力衰竭动物模型的不同阶段, 心肌细胞均有iNOS mRNA 的表达, 表达的水平在起搏的第1 周末即达到高峰, 第2 周末 有所回落并在第3 周末维持在较稳定的水平, 而在第4 周末则出现了明显的衰退。…”
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Chemical Biology of G-quadruplex and i-motif DNA: use of topologically constrained DNA by Dejeu, Jérôme, Defrancq, Eric

“…They are indeed involved in many biological processes including translation regulation, pre-mRNA processing, mRNA targeting, telomere maintenance, etc. …”
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Intra-uterine Growth Restriction Downregulates the Hepatic Toll Like Receptor-4 Expression and Function by Ozlem Equils, Sapna Singh, Semra Karaburun, Daning Lu, Manikkavasagar Thamotharan, Sherin U. Devaskar

“…IUGR had no effect on the splenic (n = 4) or intestinal (n = 4) TLR4 mRNA levels. In the liver, IUGR led to a 20% increase in baseline tumor necrosis factor (TNF)-α mRNA expression ( p < 0.03) and a 70% increase in interleukin-1β (IL-1β) mRNA expression ( p < 0.008) as compared to the control rats (CM/CP; n = 7). …”
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