Identification of biomarkers associated with ferroptosis in macrophages infected with Mycobacterium abscessus using bioinformatic tools. by Jiahua Qian, Chenghua Lu, Kai Meng, Zhihong Xu, Honghao Xue, Weijie Yang

“…To explore regulatory network relationships among hub genes, we constructed RBP-mRNA, ceRNA, and TF-mRNA networks. Additionally, a protein-protein interaction network was built, and functional enrichment analyses were conducted for the hub genes. …”
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The role of the pAbp gene encoding the cytoplasmic poly(A)-binding protein in spermatogenesis of Drosophila melanogaster by E. U. Bolobolova, E. M. Baricheva, N. V. Dorogova

“…Cytoplasmic PABPs participate in the metabolic pathways of the mRNA: translation initiation and termination, cytoplasmic polyadenylation/deadenylation, mRNA stability, mRNA degradation. …”
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Study of RNA Interference Targeting NET-1 Combination with Sorafenib for Hepatocellular Carcinoma Therapy In Vitro and In Vivo by Song He, Ying-ze Wei, Gui-lan Wang, Yu-yin Xu, Jia-ming Zhou, Yi-xin Zhang, Li Chen

“…The expressions of NET-1 mRNA and protein were detected by RT-QPCR and western blot. …”
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Comprehensive analysis of poly(A) tails in mouse testes and ovaries using Nanopore Direct RNA Sequencing by Agnieszka Czarnocka-Cieciura, Michał Brouze, Natalia Gumińska, Seweryn Mroczek, Olga Gewartowska, Paweł S. Krawczyk, Andrzej Dziembowski

“…In this context, we provide a comprehensive transcriptomic dataset focusing on mRNA poly(A) tail composition and dynamics in murine testes and ovaries. …”
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Benidipine Protects Kidney through Inhibiting ROCK1 Activity and Reducing the Epithelium-Mesenchymal Transdifferentiation in Type 1 Diabetic Rats by Ganlin Wu, Meirong Xu, Kui Xu, Yilan Hu

“…B group showed attenuated MYPT1 phosphorylation, decreased ROCK1, α-SMA protein, and ROCK1 mRNA expression and increased expression of E-cadherin protein. …”
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Role of Eukaryotic Initiation Factors during Cellular Stress and Cancer Progression by Divya Khandige Sharma, Kamiko Bressler, Harshil Patel, Nirujah Balasingam, Nehal Thakor

“…Protein synthesis can be segmented into distinct phases comprising mRNA translation initiation, elongation, and termination. …”
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Transcriptome Analysis of Long Noncoding RNAs in Toll-Like Receptor 3-Activated Mesenchymal Stem Cells by Shihua Wang, Xiaoxia Li, Robert Chunhua Zhao

“…To explore the potential involvement of lncRNAs in TLR stimulated MSCs, we performed a comprehensive lncRNA and mRNA profiling through microarray. 10.2% of lncRNAs (1733 out of 16967) and 15.1% of mRNA transcripts (1760 out of 11632) were significantly differentially expressed (absolute fold-change ≥5 , P value ≤0.05) in TLR3 stimulated MSCs. …”
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林麝<i>FASN</i>基因CDS区克隆及序列分析 by 谌颖莲 竭 航 赵贵军 封孝兰 张承露 吴佳勇 曾德军

“…为了解林麝(<i>Moschus berezovskii</i>)脂肪酸合酶(Fatty acid synthase,FASN)基因mRNA序列信息及解析麝香的生物机制,通过转录组测序、Trinity拼接、反转录PCR扩增和Singer测序获取林麝<i>FASN</i>基因mRNA序列,并对FASN蛋白序列进化树构建、二级结构、三级结构、互作蛋白、磷酸化分析及miRNAs结合预测,筛选与麝鼠(<i>Ondatra zibethicus</i>)FASN相同且与牛(<i>Bos taurus</i>)、山羊(<i>Capra hircus</i>)和绵羊(<i>Ovis aries</i>)不同的氨基酸位点作为麝香生物合成有关的关键位点。…”
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Adjusting Effects of Baicalin for Nuclear Factor-κB and Tumor Necrosis Factor-α on Rats With Caerulein-Induced Acute Pancreatitis by Dongbo Xue, Weihui Zhang, Yingmei Zhang, Haiyang Wang, Biao Zheng, Xingye Shi

“…It was shown in the results that the expression of TNF-α mRNA, activation of NF-κB, and pathological score of AP group were all obviously higher than those of control group (P<.01). …”
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Detection of c-erbB-2 mRNAs Using Dig-Labelled Oligonucleotide Probe with in situ Hybridisation in Human Breast Carcinoma: Comparison with Immunohistochemical Results by Melek Öztürk, Sema Bolkent, Selma Yilmazer, Gültekin Kaner, Hilal Ünal

“…In this study for the detection of the overexpression mRNA in situ hybridisation (ISH) and immunohistochemistry (IHC) were used. …”
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Screening of necroptosis-related genes and evaluating the prognostic capacity, clinical value, and the effect of their copy number variations in acute myeloid leukemia by Dake Wen, Ru Yan, Lin Zhang, Haoyang Zhang, Xuyang Chen, Jian Zhou

“…The expression levels of the hub-NRDEGs were validated in vitro. The mRNA-miRNA and mRNA-TF interaction networks with the hub-NRDEGs were screened using Cytoscape@. …”
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Potential Value of Circular RNA circTBC1D4 in Gastrointestinal Stromal Tumors by null Chunyan-Zou, null Yanpeng-He, null Changshun-Xie, null Fulai-Gao, null Xiaoli-Xie, null Xiukun-Chai, null Chenxi-He, null Dongqiang-Zhao

“…Nevertheless, detailed investigations of circRNA-miRNA-mRNA regulatory networks in GIST are lacking. Objective. …”
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基质金属蛋白酶-2和血管内皮生长因子在肝癌肝移植受体的表达和意义

“…【方法】 肝移植受体分为4组:供体组(n = 60),肝硬化组(n = 60),肝癌无癌栓组(n = 45)和肝癌有癌栓组 (n = 35),分别用ELISA法测定4组血MMP2和VEGF浓度;荧光定量PCR法测定4组肝脏标本MMP2 mRNA和VEGF mRNA的表达量?肝癌受体接受肝移植术后,随访了解肿瘤的复发情况,并测定血MMP2?…”
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不同剂量苯那普利对自发性高血压大鼠肾小球硬化的影响

“…【目的】 观察超大剂量血管紧张素转化酶抑制剂干预能否进一步抑制肾小球硬化65377;【方法】 30只8周龄雄性自发性高血压大鼠随机分为模型对照组(M组),大剂量(10 mg·kg-1·d-1)苯那普利治疗组(LT组),超大剂量(50 mg·kg-1·d-1)苯那普利治疗组(HT组),以10只Wistar-Kyoto大鼠(Wistar-Kyoto rats,WKY)为正常对照组(N组)65377;检测各组大鼠的血压65380;血清肌酐及24 h尿蛋白;RT-PCR和免疫组织化学方法分别检测肾组织转化生长因子-β1(transforming growth factor,TGF-β1)和纤溶酶原激活物抑制因子(Plasminogen Activator Inhibitor,PAI-1)的mRNA和蛋白表达;肾组织石蜡切片评估肾小球胶原沉积程度,采用MASSON染色65377; 【结果】 1)TGF-β1和PAI-1的mRNA和蛋白表达:LT组和HT组的TGF-β1和PAI-1 的mRNA和蛋白表达被抑制(P < 0.01);两治疗组间无统计学差异(P > 0.05)65377;2)肾小球胶原沉积:LT组和HT组的胶原面积/肾小球面积比值均明显减低(0.17 ± 0.15, 0.18 ± 0.11 vs 0.30 ± 0.28; P = 0.001);两治疗组间无统计学差异(0.17 ± 0.15 vs 0.18 ± 0.11; P=0.404)65377;【结论】 大剂量与超大剂量血管紧张素转化酶抑制剂均能降低血压,减少蛋白尿,抑制肾组织TGF-β1和PAI-1的mRNA和蛋白表达,延缓肾小球硬化;但超大剂量血管紧张素转化酶抑制剂不能进一步的抑制肾小球硬化65377;…”
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Ox-LDL诱导血管内皮细胞表达LOX-1

“…随着ox-LDL与 HUVECs作用时间的延长，LOX-I mRNA和蛋白的表达显著升高(P< 0.01), 在6-24 h内呈明显的时间－效应关 系(P< 0.01)。…”
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Comprehensive Analysis and Functional Characteristics of Differential Expression of N6-Methyladenosine Methylation Modification in the Whole Transcriptome of Rheumatoid Arthritis by Lei Wan, Jian Liu, Chuanbing Huang, Ziheng Zhu, Kun Wang, Guanghan Sun, Lei Zhu, Zhongxiang Hu

“…N6-methyladenosine (m6A) modification is the most prevalent chemical modification in eukaryotic mRNA and is associated with the development of various immune diseases. …”
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Developmental changes of skin quality from breast, back, and thigh of Pekin ducks from 1 to 6 weeks of age by Hao Li, Wenxuan Zheng, Shuo Xin, Ye Hui, Yijun Zhu, Xi Lin, Huaqi Zhang, Yongwen Zhu

“…The skin quality was increased in a quadratic manner, which was associated with the changes in mRNA expression of target genes related to cell proliferation and collagen synthesis.…”
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miRNA-1与糖尿病心肌病心肌细胞肥大相关性的初步探讨

“…心肌细胞蛋白含量随培养的时间延长而逐步增高,并明显大于正常糖浓度培养下的心肌细胞;高糖刺激时心肌细胞ANP及BNP mRNA表达变化趋势与miRNA-1表达一致,ANP mRNA表达与miRNA-1存在线性相关(r = 0.990,P = 0.01)?…”
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胆管上皮细胞表达TGF-?茁在胆汁淤积性肝病中的作用

“…【目的】 通过研究胆汁淤积性肝病不同阶段的肝脏病变65380;BDEC增殖及转化生长因β(TGF-β)的变化,以探讨胆汁淤积性肝病发生发展的可能机制65377;【方法】 ①以胆总管结扎(BDL)制作胆汁淤积性肝病大鼠模型,分为正常对照组(D0)65380;7天组(D7)及18天组(D18);②动态观察不同时间点大鼠肝脏病理变化,以Knodell组织学评分评价肝脏组织学改变;③自肝脏中分离BDEC,以实时定量PCR方法(RT-PCR)检测不同时间点肝脏组织以及分离所得BDEC中TGF-β表达的情况;④分析肝脏Knodell组织学评分与TGF-β1 mRNA表达水平间的相关性;⑤利用永生化的小鼠肝内胆管上皮细胞株(mIBEC),通过体外实验探讨TGF-β1对mIBEC增殖的作用65377; 【结果】 ①肝脏Knodell组织学评分随着淤胆时间延长而增加(P < 0.01),且胆管增殖程度随着淤胆时间延长而加重;②肝脏组织及BDEC中TGF-β165380;TGF-β2及TGF-β3的mRNA表达水平随着淤胆时间延长而显著上调;③肝脏Knodell组织学纤维化程度评分与不同时间点肝脏组织及BDEC中TGF-β1 mRNA表达水平间存在正相关(r = 0.9376,P < 0.05及r = 0.9682,P < 0.01)65377;④体外研究显示,TGF-β1可抑制mIBEC增殖65377;【结论】 ①BDL诱导的胆汁淤积性肝病中,肝脏损伤65380;胆管增殖程度及TGF-β mRNA表达水平均随淤胆时间延长而增加;②TGF-β1与BDEC的相互作用在BDL诱导的胆汁淤积性肝病发生发展中起着重要作用;③增生的BDEC中TGF-β1表达上调参与了BDL诱导的胆汁淤积性肝纤维化形成65377;…”
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Effect of Electroacupuncture on Chondrocyte Apoptosis in Knee Osteoarthritis Rat Based on PI3K/Akt Signaling Pathway by Yuanyuan ZHANG, Xinyu JIANG, Xihai LI, Mingxia WU

“…The expression of PI3K, Akt, Bcl-2, Bax mRNA and protein were detected by real-time quantitative PCR and Western blot.Results:Compared with the sham operation group, the cartilage morphology of the model group was disordered, the number of chondrocytes was significantly reduced, the matrix staining was lost, the tidal line was distorted and incomplete, Mankin's score was significantly increased (<italic>P</italic>&lt; 0.01), the expressions of IL-1β and TNF-α in the synovium were significantly increased (<italic>P</italic>&lt; 0.01), the expressions of PI3K, Akt and Bcl-2 mRNA and protein were significantly decreased (<italic>P</italic>&lt; 0.01), and the expression of Bax mRNA and protein were significantly increased (<italic>P</italic>&lt; 0.01). …”
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