Mechanism of Acupuncture Therapy on RANKL/OPG Signaling Pathway to Regulate Bone Metabolism Balance in Osteoporosis Model Rats by Qin YIN, Hong PAN, Xiaojuan DING, Xiaoguang QIN, Xiaorong GUO

“…Sp and SMI were significantly decreased(<italic>P</italic>&lt;0.05).③Apoptosis of distal bone cells: compared with the blank group, the number of apoptotic cells in the model group increased significantly;compared with the model group, the number of apoptotic cells in the distal femur of the estradiol group and the acupuncture group decreased significantly.④OPG and RANKL mRNAand protein expression in hypothalamus and femur: compared with the blank group, OPG mRNA and protein expression in hypothalamus and femur of the model group were significantly decreased, while RANKL mRNA and protein expression were significantly increased(<italic>P</italic>&lt;0.05);compared with the model group, the expression of OPG mRNA and protein in hypothalamus and femur of the acupuncture group were significantly increased, while the expression of RANKL mRNA and protein were significantly decreased(<italic>P</italic>&lt;0.05). …”
Get full text

Inhibition of Angiogenic Factor Production from Murine Mast Cells by an Antiallergic Agent (Epinastine Hydrochloride) In Vitro by K. Asano, A. Furuta, K. Kanai, S. Sakaue, H. Suzaki, T. Hisamitsu

“…Semiquantitative analysis using RT-PCR showed that EP also significantly reduced mRNA expressions for KC, TNF, and VEGF. These results strongly suggest that EP suppresses angiogenesis factor production through the inhibition of mRNA expression in mast cells and results in favorable modification of clinical conditions of allergic diseases.…”
Get full text

抵抗素诱导脐静脉内皮细胞功能异常 by 李志臻, 黎锋, 严励, 李芳萍, 李焱, 程桦, 傅祖植

“…流式细胞检测抵抗素对内皮细胞间黏附分子（ICAM-1）、血管细胞间黏附分子（VCAM-1）与活性氧簇（ROS）表达的影响；RT-PCR检测抵抗素对内皮素（ET-1）、内皮型一氧化氮合酶（eNOS）、诱导型一氧化氮合酶（iNOS）mRNA表达的影响。【结果】人脐静脉内皮细胞经人抵抗素（50ng／mL、100ng／mL）处理24h后ICAM-1和ET-1mRNA表达显著增高，而各组间VCAM-1表达、ROS生成，以及eNOS和iNOS mRNA表达无明显差别。…”
Get full text

RETRACTED ARTICLE: Histological, ultrastructural, and biochemical study on the possible role of Panax ginseng in ameliorating liver injury induced by Lambda cyhalotherin by Manal Abdul-Hamid, Hanaa M. Mohamed, Sanaa M. Abd El-Twab, Karim Zaied

“…Results LCT significantly decreased catalase (CAT), superoxide dismutase (SOD), and total thiol (T. thiol) and increased lipid peroxidation (LPO). mRNA and protein expression levels of p53 were upregulated, whereas Bcl-2 mRNA and protein expression levels were downregulated in LCT-intoxicated animals. …”
Get full text

Fat mass and obesity-associated protein expression in colorectal cancer and its influence on the biological behaviour of colorectal cancer cells by Huihui Wu, Yibo Wu, Zhishan Zhang, Qinglin Chen, Tingjin Zheng, Hui Zhong

“…Expression levels of FTO mRNA and protein in colorectal cancer and adjacent non-cancerous tissues were assessed using RT-PCR and immunohistochemistry. …”
Get full text

Statins Increase the Frequency of Circulating CD4+FOXP3+ Regulatory T Cells in Healthy Individuals by Ana Lucía Rodríguez-Perea, Carlos J. Montoya, Sven Olek, Claire A. Chougnet, Paula A. Velilla

“…We found that both statins significantly increased Treg frequency and FOXP3 mRNA levels at day 30. At day 45, Treg numbers returned to baseline values; however, TGF-β and FOXP3 mRNA levels remained high, accompanied by increased percentages of CTLA-4- and GITR-expressing Treg. …”
Get full text

Survivin 启动子控制LRIG1基因表达的重组腺病毒对人膀胱癌裸鼠移植瘤的治疗

“…与其他两组相比,Ad-Surp-LRIGl组LRIG1的mRNA和蛋白表达水平明显升高,而EGFR的mRNA和蛋白表达水平则显著降低(P < 0.01)?…”
Get full text

ＤＪ－１蛋白调控乳鼠心肌细胞内质网应激反应的研究

“…通过腺病毒转染过表达ＤＪ－１蛋白，应用荧光定量ＰＣＲ及Ｗｅｓｔｅｒｎ ｂｌｏｔｔｉｎｇ分别检测在ＰＥ及ＴＧ作用下，心肌细胞内质网应激标记物ＧＲＰ７８和ｃａｌｒｅｔｉｃｕｌｉｎ的ｍＲＮＡ及蛋白表达改变，明确ＤＪ－１对心肌细胞内质网应激的作用。 【结果】 ＰＥ、ＴＧ均可引起ＧＲＰ７８及ｃａｌｒｅｔｉｃｕｌｉｎ的ｍＲＮＡ及蛋白表达升高，提示二者均可导致心肌细胞的内质网应激反应。…”
Get full text

A Model of Insulin Resistance in Mice, Born to Diabetic Pregnancy, Is Associated with Alterations of Transcription-Related Genes in Pancreas and Epididymal Adipose Tissue by Akadiri Yessoufou, Kabirou Moutairou, Naim Akhtar Khan

“…Adult IR and control offspring were selected and we determined the mRNA expression of transcription factors known to modulate pancreatic and adipose tissue activities and inflammation. …”
Get full text

Expression and Significance of AQP3 in Cutaneous Lesions by Dongfeng Niu, Yanhua Bai, Qian Yao, Wei Hou, Lixin Zhou, Xiaozheng Huang, Chen Zhao

“…AQP3 mRNA was detected in normal epidermis, seborrheic keratosis, solar keratosis, Bowen’s disease, squamous cell carcinoma, eccrine poroma, apocrine carcinoma, and sebaceoma; however, AQP3 mRNA was absent in basal cell carcinoma, nevocellular nevus, or malignant melanoma. …”
Get full text

A NOTCH3 pathogenic variant influences osteogenesis and can be targeted by antisense oligonucleotides in induced pluripotent stem cells. by Ernesto Canalis, Jungeun Yu, Lauren Schilling, Paymaan Jafar-Nejad, Michele Carrer

“…ASOs targeting NOTCH3 decreased both NOTCH3 wild type and NOTCH36692-93insC mutant mRNA by 40% in mesenchymal and 90% in osteogenic cells. …”
Get full text

ATRA-induced NEAT1 upregulation promotes autophagy during APL cell granulocytic differentiation. by Doudou Tang, Huihui Wang, Yafeng Jiang, Mingjie Chen, Guangsen Zhang, Shangjie Wu, Yewei Wang

“…Specifically, NEAT1 binds to the RNA-binding protein TAF15, which in turn stabilizes the mRNA of both ATG10 and ATG12.…”
Get full text

Effect of Stretching Force on the Cells of Epithelial Rests of Malassez In Vitro by Teruyoshi Koshihara, Kenichi Matsuzaka, Toru Sato, Takashi Inoue

“…Expression of OPN-mRNA in the stretching group was significantly higher than that of control group at 12 and 24 hours (P<.05). …”
Get full text

Hepatic Overexpression of GRP94 in a Rabbit Model of Parenteral Nutrition-Associated Liver Disease by Xueping Zhu, Xiaomin Zhang, Lingling Yu, Yumin Xu, Xing Feng, Jian Wang

“…The RT-PCR results showed that the level of GRP94 mRNA in the liver of the TPN group was significantly higher compared with the control group. …”
Get full text

Uninephrectomy and sodium‐glucose cotransporter 2 inhibitor administration delay the onset of hyperglycemia by Yuri Sakai Ishizaki, Masao Kikuchi, Koichi Kaikita, Shouichi Fujimoto

“…There were few group differences in food intake or body weight, but blood glucose concentrations continued to rise in the sham + placebo, whereas this rise was delayed for several weeks in the UNx + placebo, and largely suppressed by dapagliflozin. SGLT2 mRNA expression was significantly lower in the UNx group, but SGLT1 mRNA expression did not significantly differ. …”
Get full text

GLP-1通过NOX4信号通路拮抗AGE诱导的人脐静脉内皮细胞氧化损伤

“…【结果】 与对照组相比,AGE可诱导细胞NOX4蛋白表达(P = 0.001)及NOX4 mRNA表达(P < 0.05)明显增高;加入GLP-1后,增高的NOX4蛋白(P = 0.003)及NOX4 mRNA(P < 0.05)明显下降?…”
Get full text

PTEN 基因影响K562细胞周期的分子机制探讨

“…【目的】 探讨抑癌基因PTEN对人慢性粒细胞白血病细胞系K562细胞周期的影响65377; 【方法】 将携带有野生型PTEN和绿色荧光蛋白的腺病毒(Ad-PTEN-GFP)及对照载体腺病毒(Ad-GFP)转染人慢性粒细胞白血病细胞系K56265377;MTT检测细胞增殖抑制率;流式细胞仪检测转染效率65380;细胞凋亡率和细胞周期的变化;荧光定量PCR(FQ-PCR)检测PTEN65380;Cyclin D165380;Cyclin D265380;CDK465380;P27Kip1 mRNA水平变化,Western blot检测上述蛋白表达水平的变化65377; 【结果】 与Ad-GFP组比较,Ad-PTEN-GFP 转染K562细胞后,细胞凋亡率最高为30%,细胞周期显示:G0/G1期细胞比例由54.9%增加至78.5%,G2/M期比例由30.2%降至13.6%,Cyclin D165380;Cyclin D265380;CDK4 mRNA及CyclinD1蛋白表达降低,P27Kip1 mRNA及蛋白表达增加65377; 【结论】 PTEN基因高表达可以促进K562细胞凋亡,并通过抑制Cyclin D165380;Cyclin D265380;CDK4及增加P27Kip1表达使细胞周期阻滞在G0/G1期65377;…”
Get full text

DSIF factor Spt5 coordinates transcription, maturation and exoribonucleolysis of RNA polymerase II transcripts by Krzysztof Kuś, Loic Carrique, Tea Kecman, Marjorie Fournier, Sarah Sayed Hassanein, Ebru Aydin, Cornelia Kilchert, Jonathan M. Grimes, Lidia Vasiljeva

“…Abstract Precursor messenger RNA (pre-mRNA) is processed into its functional form during RNA polymerase II (Pol II) transcription. …”
Get full text

Chi-miR-4110 promotes granulosa cell apoptosis by targeting Sma- and Mad-related protein 2 (Smad2) in the caprine ovary. by Xiaopeng An, Yuxuan Song, Jinxing Hou, Yue Zhang, Kaiwen Chen, Haidong Ma, Xinyan Zhao, Guang Li, Kexin Gao, Shan Wang, Binyun Cao, Yueyu Bai

“…Results revealed that chi-miR-4110 decreased mRNA and protein levels of Smad2 by targeting its 3'-untranslated region (3'UTR). …”
Get full text

Expression of lipocortins in human bronchial epithelial cells: effects of IL-1β , TNF-α, LPS and dexamethasone by M. M. Verheggen, H. I. M. de Bont, P. W. C. Adriaansen-Soeting, B. J. A. Goense, C. J. A. M. Tak, H. C. Hoogsteden, P. Th. W. van Hal, M. A. Versnel

“…No induction of lipocortin I or II mRNA or protein was observed after incubation with dexamethasone. …”
Get full text