CSF-1 自背根节向脊髓转运对长春新碱诱导神经病理性痛大鼠小胶质细胞活化及其炎症的影响 by 付宝军, 姜静静, 黄玉琼, 林宗航, 李恒

“…分别采用机械缩足反射阈值（MWT）和热缩足反射潜伏期（TWL）评价大鼠机械痛敏和热痛敏；Western   blotting  法和免疫荧光化学检测CSF-1 以及小胶质细胞标志物Iba1 表达；RT-PCR 法检测CSF-1 mRNA 和Iba1 mRNA 表达；ELISA 法检测TNF-α、IL-6 和IL-1β。【结果】与Control 组比较，CINP 组大鼠在长春新碱首次注射后第3、5、7 天MWT 和TWL 明显降低（P<0.001）；与 CINP 组比较，CINP+anti 组 MWT 和 TWL 在第 5、7 天明显升高（P<0.001）；与 Control 组比较， CINP 组背根节CSF-1 蛋白及mRNA 表达、脊髓Iba1mRNA 表达、脊髓CSF-1 和Iba1 蛋白表达、脊髓Iba1 和背根节CSF-1 荧光强度、脊髓 TNF-α、IL-6 和 IL-1β表达明显上调（P<0.01，P<0.001）；与 CINP 组比较，CINP+anti 组背根节和脊髓 CSF-1 蛋白表达、脊髓 Iba1 蛋白及 mRNA 表达、脊髓 Iba1 和背根节 CSF-1 荧光强度、脊髓 TNF-α、IL-6 和IL-1β表达明显下调（P<0.05，P<0.01，P<0.001）。…”
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前列腺素Ｅ２体外主要通过活化受体ＥＰ４促进人ＣＤ３４＋ 细胞增殖

“…【结果】 人ＣＤ３４＋细胞可表达ＰＧＥ２的四个亚受体，但主要表达受体ＥＰ２和ＥＰ４；ＰＧＥ２作用后，ＥＰ２和ＥＰ４ ｍＲＮＡ表达量较对照组明显升高，而ＥＰ１和ＥＰ３的ｍＲＮＡ表达变化不明显；ＥＰ４Ａ可促进人ＣＤ３４＋细胞各系造血集落形成；ＥＰ４Ａ可促进人 ＣＤ３４＋细胞进入细胞周期，使Ｇ２／Ｍ期比例增加； ＥＰ４Ａ可促进凋亡抑制蛋白ｓｕｒｖｉｖｉｎ的ｍＲＮＡ和蛋白表达、β－ｃａｔｅｎｉｎ蛋白表达。…”
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Identification of a functional vitamin D response element in the promoter of goose anti-Müllerian hormone gene by Rong Chen, Chen Chen, Yifei Qin, Jie Liu, Mingming Lei, Huanxi Zhu, Zhendan Shi

“…Anti-Müllerian hormone (AMH) plays an important role in avian ovarian follicle development. The high mRNA expression of AMH in avian ovarian prehierarchical follicles helps prevent premature granulosa cell differentiation. …”
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Analysis of long noncoding gene expression and its interactions with protein-coding genes in vascular endothelial cells in keloids by Yunxiang Luo, Zi Ye, Yi Li, Chau Wei Wong, Shuqia Xu, Yu Deng, Zhicheng Su, Xueqing Li, Yingxiong Huang, Bing Han

“…Expression differences in mRNA and lncRNA between keloids and normal skin were calculated. …”
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辛伐他汀含药血清对U937单核巨噬细胞MMP-9及TIMP-1表达与分泌的影响 by 刘金来, 朱强锋, 郝宝顺

“…分别予不同浓度含药血清（5%、10%、20%）培养U937单核细胞源巨噬细胞24 h,应用逆转录聚合酶链反应检测MMP-9 mRNA及TIMP-1 mRNA的表达,以及应用酶联免疫吸附试验检测MMP-9及TIMP-1蛋白的分泌。…”
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Impact of Exercise on Tramadol-Conditioned Place Preference by Haneen Amawi, Alaa M. Hammad, Aseel Abdullah Ibrahim, Nosyba Alsbih, Frank Scott Hall, Fawaz Alasmari, Bahaa Al-Trad

“…Additionally, the TRA-induced elevations in <i>Il-1β</i> mRNA expression were reversed by all durations of exercise in Hipp. …”
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The RNA M5C methyltransferase NSUN2 promotes progression of hepatocellular carcinoma by enhancing PKM2-mediated glycolysis by Qin Qi, Rui Zhong, Yan Huang, Yong Tang, Xiao-wen Zhang, Chang Liu, Chun-fang Gao, Lin Zhou, Jian Yu, Lu-yi Wu

“…Specifically, NSUN2 could stabilize PKM2 mRNA by increasing the m5C level of the m5C site C773 in the 3′-UTR of PKM2 mRNA. …”
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MiR-107-3p/Atp6v0e1 contributes to protective effects of two selenium-containing peptides,TSeMMM and SeMDPGQQ on lead-induced neurotoxicity by Yong Fang, Tianhang Huang, Jian Wu, Xieqi Luo, Fengjiao Fan, Peng Li, Jian Ding, Xinyang Sun

“…However, the crosstalk between mRNA and microRNAs (miRNA) involved in the neuroprotection mechanism remains to be elucidated. …”
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Effects of neonatal handling on behavior and stress-response in rats selected for reaction towards humans by Yu. E. Herbeck, O. A. Amelkina, M. Yu. Konoshenko, S. G. Shikhevich, R. G. Gulevich, R. V. Kozhemyakina, I. Z. Plyusnina, I. N. Oskina

“…Neonatal handling decreased the amount of mRNA for the glucocorticoid receptor (GR) in the hippocampus of aggressive rats but did not affect significantly the amount of mRNA for the corticotropin-releasing hormone (CRH) in the hypothalamus. …”
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Inhibitory Effects of a Novel PPAR-γ Agonist MEKT1 on Pomc Expression/ACTH Secretion in AtT20 Cells by Rehana Parvin, Erika Noro, Akiko Saito-Hakoda, Hiroki Shimada, Susumu Suzuki, Kyoko Shimizu, Hiroyuki Miyachi, Atsushi Yokoyama, Akira Sugawara

“…Pomc mRNA expression and promoter activity were significantly inhibited by MEKT1 at 10 μM compared to rosiglitazone and pioglitazone. …”
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MiR-595 and Cldnd1: Potential related factors for bone loss in postmenopausal women with hip osteoporotic fracture. by Sun Jingyue, Liu Peixin, Wang Xiao

“…<h4>Background</h4>Recently researches have reported that miRNA and its target genes are associated with osteoporosis. MiRNA and mRNA might be potential diagnostic markers for osteoporosis.…”
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血管紧张素II对足细胞葡萄糖转运蛋白1的表达及 膜转位的影响

“…【目的】 观察血管紧张素II(AngII)对体外培养的小鼠足细胞葡萄糖转运蛋白1(GLUT1)的表达及膜转位的影响?【方法】 AngII对GLUT1mRNA和GLUT1蛋白表达的检测将体外培养的足细胞分为4组:对照组(C)?低剂量组(AngII 10-10 mol/L)?…”
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Palmitic acid-induced cell death: impact of endoplasmic reticulum and oxidative stress, mitigated by L-citrulline by Md. Rezwanul Habib, Yukako Tokutake, Shinichi Yonekura

“…PA increased intracellular ROS levels and NRF2 mRNA expression, whereas L-citrulline pretreatment remarkably reduced these levels. …”
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Anti-apoptosis Effect of Gualou Guizhi Granule on Neurons in Ischemic Stroke Rats and Primary Hippocampal Neurons by Yu LIN, Wei XU, Yuqin ZHANG, Huang LI, Wen XU

“…After the treatment with GLGZG, the down-regulated expression of Caspase-3, Bax mRNA level(<italic>P</italic>&lt;0.05, <italic>P</italic>&lt;0.01)and up-regulated expression of Bcl-2 mRNA(<italic>P</italic>&lt;0.05)were observed in primary hippocampal neurons in the above three GLGZG grous in comparison with that in the glutamate group. …”
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IL-22对类风湿关节炎成纤维样滑膜细胞凋亡的影响

“…利用荧光定量RT-PCR（qRT-PCR）检测Bcl-2 mRNA和Bcl-XL mRNA、Western Blotting检测Bcl-2蛋白在RA、骨关节炎（OA）和创伤（Trauma）患者中FLS的表达水平。…”
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大蒜素对神经母细胞瘤生长的抑制作用

“…【目的】 探讨大蒜素对神经母细胞瘤生长的影响及相关机制65377;【方法】 MTT法测定不同浓度大蒜素对神经母细胞瘤细胞生长的影响;建立裸鼠人神经母细胞瘤皮下移植瘤模型24只(n = 8),随机分为对照组65380;大蒜素Ⅰ组(1 mg8226;kg-18226;d-1)和大蒜素Ⅱ组(10 mg8226;kg-18226;d-1),观察其对肿瘤生长的影响,荧光定量PCR法测定大蒜素对裸鼠皮下移植瘤VEGF mRNA表达的影响;ELISA法测定裸鼠血浆VEGF浓度;免疫组化法观察肿瘤微血管密度65377;【结果】 不同浓度的大蒜素均能抑制神经母细胞瘤细胞的生长,高浓度的大蒜素能直接杀死肿瘤细胞;大剂量和小剂量大蒜素对裸鼠神经母细胞瘤皮下移植瘤均有抑制作用65377;对照组65380;大蒜素Ⅰ组和Ⅱ组的肿瘤体积为(2143 ± 127) mm365380;(1041 ± 89) mm3和(904 ± 65) mm3 (P < 0.05);大蒜素能下调神经母细胞瘤VEGF mRNA转录,对照组65380;大蒜素Ⅰ组和Ⅱ组VEGF mRNA表达量分别为(8.4 ± 0.8)65380;(6.1 ± 0.7)和(5.1 ± 0.5);大蒜素能够抑制肿瘤细胞VEGF的表达,对照组65380;大蒜素Ⅰ组和Ⅱ组裸鼠血VEGF浓度分别为(78 ± 10)65380;(45 ± 8)和(35 ± 7) pg/mL;大蒜素可以减少神经母细胞瘤的微血管密度65377;【结论】 大蒜素在体内和体外均可抑制神经母细胞瘤细胞的生长,其机制与大蒜素抑制肿瘤细胞增殖和下调神经母细胞瘤VEGF mRNA的转录和蛋白表达有关65377;…”
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Effects of the Replacement of Dietary Fishmeal by the Blend of Tenebrio molitor Meal, Chlorella Meal, Clostridium Autoethanogenum Protein, and Cottonseed Protein Concentrate on Gro... by Yongning Yu, Xing Wang, Junyan Jin, Dong Han, Xiaoming Zhu, Haokun Liu, Zhimin Zhang, Yunxia Yang, Shouqi Xie

“…The 100% substitution of FM with BLEND A significantly upregulated the mRNA expression of intestinal proinflammatory cytokines and downregulated the mRNA expression of anti-inflammatory cytokine il-10 (P <0.05), reduced intestinal villus height (P<0.05), and decreased protein apparent digestibility coefficient (P<0.05). …”
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Angesinenolide B, A Phthalide Dimeric Peroxide, Exerts Anti-Inflammatory Properties by Suppressing MAPK/STATs Signaling Pathways and ROS Production by Zhang L, Liu Y, Wang H, Guo S, Lv J

“…In vivo, ROS generation was explored using fluorescent probe DCFH-DA, and the TNF-α and IL-6 mRNA expressions were also evaluated in CuSO4-induced zebrafish inflammation model.Results: ASB treatment was found to suppress the levels of NO, TNF-α, IL-6 and ROS, restrain the expressions of iNOS and COX-2 proteins and mRNA, as well as down-regulate MAPK and STATs signaling pathways in LPS-stimulated RAW264.7 cells. …”
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Treatment of Cancer Gene Changes in Chronic Myeloid Leukemia by Big Data Analysis Platform-Based Dasatinib by Linlin Song, Qi Li, Hui Shi, Pengxia Zhang

“…Messenger ribonucleic acid (mRNA) datasets of CML patients were selected from cancer genomic map, including 120 samples and 20,614 mRNA in total. …”
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Fasting Upregulates PPAR𝛼 Target Genes in Brain and Influences Pituitary Hormone Expression in a PPAR𝛼 Dependent Manner by Bettina König, Christine Rauer, Susann Rosenbaum, Corinna Brandsch, Klaus Eder, Gabriele I. Stangl

“…Furthermore, pituitary prolactin and luteinizing hormone-𝛽 mRNA concentrations were increased upon fasting in wild-type mice but not in mice lacking PPAR𝛼. …”
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