BMI1, ALDH1A1, and CD133 Transcripts Connect Epithelial-Mesenchymal Transition to Cancer Stem Cells in Lung Carcinoma by Ana Koren, Matija Rijavec, Izidor Kern, Eva Sodja, Peter Korosec, Tanja Cufer

“…BMI1 mRNA expression in tumors positively correlated with BMI1 mRNA expression in blood. …”
Get full text

Localized molecular chaperone synthesis maintains neuronal dendrite proteostasis by Célia Alecki, Javeria Rizwan, Phuong Le, Suleima Jacob-Tomas, Mario Fernandez Comaduran, Morgane Verbrugghe, Jia Ming Stella Xu, Sandra Minotti, James Lynch, Jeetayu Biswas, Tad Wu, Heather D. Durham, Gene W. Yeo, Maria Vera

“…The most abundant dendritic chaperone mRNA encodes a constitutive heat shock protein 70 family member (HSPA8). …”
Get full text

Food intake regulation during pregnancy and lactation in mice with reduced activity of the melanocortin system by E. N. Makarova, I. V. Romanova, N. M. Bazhan

“…Pregnant Ау/a and a/a mice showed different patterns of food intake and neuropeptide expressions. NPY mRNA levels increased during pregnancy only in a/a mice, while AgRP mRNA levels increased in both genotypes being lower in Ау/a then in a/a mice. …”
Get full text

Renin-angiotensin-aldosterone system in ISIAH rats with stressinduced arterial hypertension by A. D. Dubinina, E. V. Antonov, L. A. Fedoseeva, E. N. Pivovarova, A. L. Markel, L. N. Ivanova

“…By contrast, a significant increase of RAS genes expression was found in the brain tissues. The mRNA of the Ren gene was increased in the hypothalamus, and the mRNA of Ace gene was increased in the brain stem of the ISIAH rats. …”
Get full text

β-Catenin Regulation in Sporadic Colorectal Carcinogenesis: Not as Simple as APC by Ernst Fredericks, Gill Dealtry, Saartjie Roux

“…IL-17A mRNA was significantly reduced in IBS and CRC but unchanged in IBD. …”
Get full text

1-磷酸-神经鞘氨醇预防化疗大鼠卵巢功能损害的实验研究 by 彭萍, 杨冬梓, 莫亚勤

“…卵巢组织中,S1P两组Bcl-2 mRNA的表达明显高于对照组及CTX组(P<0.01),Bax mRNA表达明显低于对照组及CTX组(P<0.01);而CTX组的Bcl-2 mRNA表达明显低于对照组及S1P两组(P<0.01),BaxmRNA表达明显高于对照组及及S1P两组(P<0.01)。…”
Get full text

Regulatory Effect of Pyroptosis Related Genes Expression in Adipose Tissue of Aging Rats by Long-Time Weight-Bearing Running by GONG Lijing, TANG Shuning, YANG Luyao, SU Hao, NI Zhen, FU Pengyu

“…The relative expression levels of ASC, NF-κB, IL-1β and NLRP3 mRNA in pWAT of rats in the resistance training group were lower than those in the control group (<italic>P</italic>&lt;0.05), and the relative expression levels of NF-κB and NLRP3 mRNA were lower than those in the baseline group, and the relative expression level of Caspase1 mRNA was higher than that in the baseline group (<italic>P</italic>&lt;0.05).The relative expression levels of NF-κB and IL-1β mRNA in sWAT of rats in the resistance group were lower than those in the control group, and the relative expression levels of ASC, NF-κB and Caspase1 mRNA were higher than those in the baseline group (<italic>P</italic>&lt;0.05). (5) After 32 weeks of intervention, the IOD values of NLRP3, Caspase1, GSDMD and IL-1β in pWAT of rats in the control group were higher than those in the baseline group (<italic>P</italic>&lt;0.05). …”
Get full text

Effect of Ginkgolide B on Non-Alcoholic Fatty Liver Disease based on Notch Signaling Pathway by Hong YU, Junzi WU, Bo SONG, Jun LI

“…Its mechanism may be that Ginkgolide B could regulate the Notch signaling pathway by inhibiting Notch-3, Hes-1 protein and mRNA expression in liver tissue.…”
Get full text

ＦＧＦ２１在小鼠非酒精性脂肪肝病程中的变化及与 ＳＲＥＢＰ的相关性 by 郭云蔚

“…与素食喂养小鼠相比，小鼠肝组织ＦＧＦ２１ ｍＲＮＡ水平在高脂饮食喂养至９周和１８周时明显升高（均Ｐ ＜ ０．０５），与高脂饮食的时间呈正相关（ｒ ＝ ０．９５２，Ｐ ＝ ０．０００）并同时与ＳＲＥＢＰ ｍＲＮＡ 水平正相关（ｒ ＝ ０．７２５，Ｐ ＝ ０．０００）。…”
Get full text

生长激素上调性激素抑制下青春期大鼠生长板原代软骨细胞表皮生长因子的表达

“…【目的】 研究生长激素(GH)对性激素抑制下的离体青春期大鼠生长板软骨细胞表皮生长因子(EGF)mRNA表达及其蛋白合成65380;分泌的影响65377;【方法】 3周龄雌性SD大鼠开始注射促性腺激素释放激素拟似物(GnRHa)至7周龄,随后分离培养5 ~ 8只大鼠胫骨生长板原代软骨细胞,分为时效组和量效组65377;采用四氮甲基唑蓝(MTT)以及免疫组化法测定增殖细胞核抗原(PCNA),观察不同剂量65380;不同干预时间的生长激素对性激素抑制下的离体青春期大鼠生长板软骨细胞增殖的影响65377;用RT-PCR法检测软骨细胞EGF mRNA的表达,酶联免疫吸附法(ELISA)测定EGF的表达65377;【结果】 生长激素以时效和量效作用方式对雌激素受抑的离体青春期大鼠生长板软骨细胞增殖呈双相型影响65377;GH作用6 h后软骨细胞的EGF mRNA表达显著增加(与0 h比较P < 0.05),且从6 h到24 h间存在明显的时间依赖性(组间P < 0.05),在18 h达到最高峰65377;GH作用12 h后软骨细胞分泌入培养基中的的EGF表达显著增加(与0 h比较P < 0.05),且从12 h到48 h间存在明显的时间依赖性(组间P < 0.05),在36 h达到最高峰65377;量效关系显示50 ng/mL浓度的GH开始能显著增加培养基中EGF mRNA和蛋白的表达,且从50 ng/mL至1 000 ng/mL间存在明显的浓度依赖性(组间P < 0.05),在100 ng/mL时表达达到最高峰65377;【结论】 生长激素能促进性激素抑制下的离体青春期大鼠生长板软骨细胞的增殖及EGF mRNA和蛋白的表达65377;…”
Get full text

Effect of Electroacupuncture on Chondrocyte Apoptosis and Cartilage Matrix of Experimental Rats with Knee Osteoarthritis by Guangwen WU, Chunsong ZHENG, Xihai LI, Qiuying LIN, Yichen DAI, Qiuxiang LIN, Yuanyuan ZHANG, Mingxia WU

“…The cartilages of left tibial plateau were obtained, and saved in liquid nitrogen, and then measured the mRNA and protein levels of Bcl-2 and Bax by RT-PCR and Western blot, respectively.Results:Compared with the blank group, the chondrocyte apoptosis rate in the model group was significantly increased (<italic>P</italic>&lt;0.05); the mRNA and protein expression of Bcl-2 was decreased (<italic>P</italic>&lt;0.05), and the mRNA and protein expression of Bax was significantly increased (<italic>P</italic>&lt;0.05). …”
Get full text

诱导型一氧化氮合酶和血管内皮生长因子在舌癌细胞株中的表达 by 曾曙光, 陈伟良, 杨岚, 邱钧琦, 张积仁, 章锦才, 艾伟健, 郑俊发

“…【方法】采用逆转录-聚合酶链反应（RT-PCR）法和SP免疫组化方法,对舌鳞癌细胞株Tca8113中iNOS和VEGF mRNA和蛋白表达情况进行检测。【结果】RT-PCR检测到舌鳞癌Tca8113细胞株中iNOS／VEGF mRNA条带明显,SP免疫组化检测到iNOS／VEGF蛋白在舌癌细胞胞浆中呈强阳性表达。…”
Get full text

Regulation of Annexin I in Rheumatoid Synovial Cells by Glucocorticoids and Interleukin-1 by Eric F. Morand, Pam Hall, Paul Hutchinson, Yuan H. Yang

“…DEX increased FLS annexin I protein translocation and mRNA expression. Interleukin (IL)-1β also induced annexin I translocation and mRNA but also increased intracellular protein. …”
Get full text

Iron-mediated post-transcriptional regulation in Toxoplasma gondii. by Megan A Sloan, Adam Scott, Dana Aghabi, Lucia Mrvova, Clare R Harding

“…The accumulation of transcript is likely driven by an increased reporter mRNA stability under low iron. Interestingly, we find iron-mediated changes in mRNA stability in around 400 genes. …”
Get full text

Cloning of HSP90, expression and localization of HSP70/90 in different tissues including lactating/non-lactating yak (Bos grunniens) breast tissue. by Penggang Liu, Sijiu Yu, Yan Cui, Junfeng He, Chuan Yu, Zexing Wen, Yangyang Pan, Kun Yang, Liangli Song, Xue Yang

“…The aim of this study is to investigate the expression and localization of HSP70/90 in different tissues and explore the regulation effects of HSP70/90 at lactation period of female yaks. HSP90 mRNA was cloned from the heart samples of female yaks, Quantitative real-time (qRT-PCR), Western blotting (WB), immunohistochemistry and immunofluorescence assays were utilized to analyze the expressions of HSP70/90 mRNA and protein in different tissues. …”
Get full text

反复种植失败患者子宫内膜“种植窗”时期催产素受体的表达 by 夏婷婷, 李洁, 张媛, 习羽, 欧建平

“…结果RIF妇女组子宫内膜组织分泌中期OTR蛋白质和mRNA显著高于正常妇女组（<italic>P </italic>&lt; 0.05）。正常妇女月经周期子宫内膜组织存在OTR/OTR mRNA的周期性表达。…”
Get full text

Identification of mitophagy-related key genes and their correlation with immune cell infiltration in acute myocardial infarction via bioinformatics analysis by Zulong Sheng, Rui Zhang, Zhenjun Ji, Zhuyuan Liu, Yaqing Zhou

“…Moreover, we found a lower abundance of immune cells such as gamma delta T and natural killer cells in the high-risk group, and the expression of key genes showed a significant correlation with immune infiltration levels in both groups. Finally, 64 miRNA–mRNA pairs, 75 TF–mRNA pairs, 119 RNA-binding protein–mRNA pairs, and 32 drug–mRNA pairs were obtained in the interaction networks.ConclusionsIn total, 12 key MRDEGs were identified and a risk model was constructed for AMI diagnosis. …”
Get full text

Alterations in Mucin-Associated Gene Expression on the Ocular Surface in Active and Stable Stages of Atopic and Vernal Keratoconjunctivitis by Mariko Horinaka, Jun Shoji, Akiko Tomioka, Yukiko Tonozuka, Noriko Inada, Satoru Yamagami

“…To evaluate the presence of ocular surface mucin in patients with atopic and vernal keratoconjunctivitis (AKC/VKC), we investigated the mRNA expression levels of SAM-pointed domain-containing ETS-like factor (SPDEF) and mucin-related genes on the ocular surface. …”
Get full text

Clinicopathologic Significance of CXCL12 and CXCR4 Expressions in Patients with Colorectal Cancer by Naomi Yoshuantari, Didik Setyo Heriyanto, Susanna Hilda Hutajulu, Johan Kurnianda, Ahmad Ghozali

“…CXCR4 mRNA expression was inversely correlated in tumours with a lymphatic invasion.…”
Get full text

阿霉素肾病大鼠肾组织中nephrin和CD2AP的表达及意义

“…【结果】 ①模型组大鼠7 d时nephrin mRNA表达增加,14 d时其蛋白表达(9.44 ± 0.49)亦显著增加;CD2AP表达增加时间较晚,28 d时其mRNA和蛋白表达(7.88 ± 1.01)才显著增加。…”
Get full text