Interleukin 6 Function in the Skin and Isolated Keratinocytes Is Modulated by Hyperglycemia by Eric G. Lee, Lerin R. Luckett-Chastain, Kaitlin N. Calhoun, Benjamin Frempah, Anja Bastian, Randle M. Gallucci

“…Wound closure, protein, and mRNA expression were assessed up to 13 days of postwounding. …”
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The role of long non-coding RNA LINC00839 in oral squamous cell carcinoma based on bioinformatics and experimental research by Ashkan Kalantary-Charvadeh, Saman Morovat, Somayeh Aslani, Nasrin Ziamajidi, Amirnader Emami Razavi, Roghayeh Abbasalipourkabir

“…Using The Cancer Genome Atlas, we analyzed lncRNA, miRNA, and mRNA sequencing data for OSCC. Different online tools were applied to detect lncRNA-related miRNAs and their target mRNAs, forming a lncRNA/miRNA/mRNA axis. …”
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基于生物信息学途径评估 UBE2 家族及 UBE2T 基因在肺腺癌中的表达及临床意义 by 杨豪帅, 赖慧娜, 饶怡茹, 林晓平

“…【结论】UBE2家族与肺腺癌临床病理特征相关。UBE2T mRNA有望成为肺腺癌新的生物标志物。…”
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Mechanism of Swimming Exercise Intervention on Rats with Vascular Dementia Based on PERK/ATF4/CHOP Pathway by LIU Fang, LI Mengxing, WANG Yu, TANG Wei

“…The mechanism may be related to the regulation of the PERK/ATF4/CHOP pathway, suppressing the expression of ERS-related proteins PERK, ATF4, CHOP mRNA and protein in the hippocampal region of rats with VD.…”
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T-bet基因转染对哮喘小鼠脾脏Th1/Th2平衡的影响

“…【目的】 观察气道内T-bet基因转染对哮喘小鼠脾脏淋巴细胞Th1/Th2平衡65380;T-bet及GATA-3 mRNA表达的影响65377; 【方法】 将C57BL/6小鼠32只随机分为4组,每组8只,分别为正常对照组(A组)65380;哮喘模型组(B组)65380;空质粒干预组(C组) 和T-bet质粒干预组(D组)65377;卵白蛋白(OVA) 抗原溶液腹腔注射致敏,滴鼻造模65377;空质粒组和T-bet质粒干预组OVA激发24 h前,分别经鼻滴入50 μg空质粒65380;重组T-bet质粒,对照组用生理盐水代替OVA65377;最后一次滴鼻激发48 h后处死小鼠,流式细胞仪检测各组实验小鼠的脾脏淋巴细胞的Th1/Th2, RT-PCR检测小鼠脾脏淋巴细胞的T-bet及GATA-3mRNA表达65377; 【结果】 哮喘小鼠气道转染pcDNA3-T-bet质粒后:①流式细胞仪检测发现,脾脏Th1百分率较哮喘模型组显著升高[(2.29 ± 1.55)% vs. (1.93 ± 1.14)%,P﹤0.05],Th2百分率显著降低[(0.93 ± 0.64)% vs. (1.63 ± 0.59)%];②RT-PCR检测发现脾脏淋巴细胞转录因子T-bet mRNA表达水平显著增加(0.53 ± 0.027 vs. 0.28 ± 0.035,P﹤0.05), GATA-3 mRNA表达水平显著降低(0.24 ± 0.022 vs. 0.58 ± 0.038,P﹤0.05)65377;而pcDNA3空质粒干预后与哮喘模型组比较无显著差异65377;【结论】 气道内转导pcDNA3-T-bet质粒后,可显著上调哮喘小鼠体内T-betmRNA表达,下调GATA-3 mRNA表达,有效改善哮喘小鼠体内Th1/Th2比例失衡,从而抑制哮喘小鼠的气道炎症,为哮喘的T-bet基因治疗提供依据65377;…”
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旋毛虫排泄分泌抗原53-ku重组蛋白上调M2型肺泡巨噬细胞减轻脓毒症小鼠急性肺损伤 by 关开泮, 刘志豪, 廖瑾莉, 曾庆理

“…LPS组小鼠肺泡灌洗液IL-6较空白组升高，而IL-4下降，灌洗巨噬细胞的TNF-α，iN? OS的mRNA表达水平较空白组明显升高，而IL-10，Arg-1的mRNA表达水平下降，表现出M1表型极化的特点；经rTsP53蛋 白治疗的脓毒症小鼠肺泡灌洗液 IL-6 浓度下降，IL-4 水平上升，灌洗巨噬细胞向 M2 表型极化，表现为 IL-10，Arg-1 的 mRNA表达水平较脓毒症组明显升高，而iNOS的mRNA表达水平下降。…”
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Expression of IL-18, IL-18 Binding Protein, and IL-18 Receptor by Normal and Cancerous Human Ovarian Tissues: Possible Implication of IL-18 in the Pathogenesis of Ovarian Carcinoma by Liat Medina, Alex Rabinovich, Benjamin Piura, Victor Dyomin, Ruthy Shaco Levy, Mahmoud Huleihel

“…Cancerous ovarian tissues expressed significantly higher IL-18 mRNA levels (P=0.025), while there was no difference in the expression of IL-18BP mRNA and IL-18R mRNA between cancerous and normal ovarian tissues. …”
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大肠癌组织CD137L的表达及意义

“…【目的】 检测大肠癌组织及癌旁组织中CD137L mRNA及蛋白的表达情况,探讨其在大肠癌发生进展中的意义65377; 【方法】 运用实时荧光定量逆转录聚合酶链反应及冰冻切片免疫组化的方法检测大肠癌组织及癌旁组织中CD137L的表达情况,并分析其表达情况与各临床因素的相关性65377; 【结果】 54例大肠癌组织中CD137L mRNA的相对表达水平为 0.035 ± 0.013,显著高于癌旁组织的0.008 ± 0.005(P < 0.05)65377;但在不同性别65380;年龄65380;肿瘤发生部位65380;Dukes分期65380;病理分化及血清CEA水平情况下,CD137L mRNA的表达水平无显著性差异(P > 0.05)65377;随机抽取8例大肠癌肿瘤组织及3例癌旁组织,进行免疫组化检测,结果显示6例CD137L蛋白呈阳性表达,CD137L表达于肿瘤细胞表面;3例癌旁组织该分子的表达均为阴性65377;【结论】 CD137L mRNA在大肠癌组织中高表达,CD137L分子表达于大肠癌肿瘤细胞表面,提示该分子可能在大肠癌的发生进展中发挥作用65377;…”
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METTL3 regulates autophagy of hypoxia-induced cardiomyocytes by targeting ATG7 by Linnan Li, Hao Cheng, Yufei Zhou, Di Zhao, Xiaoxue Zhang, Yajun Wang, Jianying Ma, Junbo Ge

“…Abstract N6-methyladenosine (m6A) mRNA modification is the most common mRNA internal modification in eukaryotes, which participates in a variety of biological processes. …”
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The Effects of Pitavastatin on Nuclear Factor-Kappa B and ICAM-1 in Human Saphenous Vein Graft Endothelial Culture by Bulent Demir, Burak Onal, Sibel Ozyazgan, Esra Demir, Vedat Bakuy, Ahmet Gokhan Akkan

“…Pitavastatin increases ICAM-1 mRNA expression in saphenous vein endothelial cells. …”
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心衰鼠肺水通道蛋白表达变化及内皮素受体拮抗剂对其的影响 by 陈筱潮, 刘英梅, 张旭明

“…测定 血液动力学参数, 放射免疫法(RIA)测血浆内皮素-1 浓度, 逆转录-聚合酶链反应(RT-PCR)法定量检测肺组织AQP4 和AQP5 m RNA 的表达。【结果】空白对照组、心衰组和心衰治疗组肺组织AQP4 m RNA 的相对水平为(0.56 ±0.12)、(0.20 ±0.08)及 (0.36 ±0.14), P <0.001 ;AQP5 mRNA 相对水平为(0.64 ±0.17),(0.09 ±0.03)及(0.38±0.12), P <0.001 。…”
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Butyric acid alleviates LPS-induced intestinal mucosal barrier damage by inhibiting the RhoA/ROCK2/MLCK signaling pathway in Caco2 cells. by Luqiong Liu, Tong Chen, Zhenrong Xie, Yongjin Zhang, Chenglu He, Yongkun Huang

“…We then applied the ROCK2 inhibitor Y-27632 and reevaluated the TEER, FD-4 flux, and mRNA, and protein expression of ZO-1, Occludin, RhoA, ROCK2, and MLCK, as well as their distribution in Caco2 cells. …”
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IgA肾病患者血清IgA1对足细胞凋亡的影响

“…与对照相比,IgAN患者和健康对照来源的aIgA1诱导足细胞Fas mRNA 升高2.4倍(P < 0.05),而Bcl-2 mRNA和Bax mRNA无显著性变化(P > 0.05)。…”
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抑制 NAD(P)H 氧化酶表达和活性对大鼠糖尿病肾病的影响

“…肾质量指数等?【结果】 糖尿病大鼠肾p22phox mRNA的表达较正常大鼠明显升高(P < 0.05), apocynin治疗不影响肾p22phox mRNA 的表达;福辛普利治疗4周,明显降低了肾p22phox mRNA表达(P < 0.05);apoc-ynin和福辛普利均显著降低了肾小球细胞外基质蛋白?…”
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脑钠肽对压力负荷诱导的小鼠心力衰竭的影响及作用机制 by 刘帅烨, 周越, 何昕, 黄艺仪, 陈艺莉, 何建桂

“…构建模型4周后通过超声心动图、心脏及肺脏质量比值来检测心脏功能 及结构变化，并使用Q-PCR技术来检测心肌肥厚标志物（ANP）mRNA水平。通过免疫荧光技术观察心肌组织内 毛细血管的变化，Image-J软件测量毛细血管密度和毛细血管/心肌细胞比值，Q-PCR和Western-Blotting 检测心 肌组织内血管新生因子（AngⅠ，VEGFA）mRNA 转录和蛋白表达水平。…”
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Sulfatase modifying factor 2 as a predictive biomarker for urothelial carcinoma by Wei-Ting Kuo, Yi-Chen Lee, Jia-Bin Liao, Ching-Jiunn Tseng, Yi-Fang Yang

“…Knockdown of SUMF2 significantly reduced the migration and invasion abilities of 5637 cells. Furthermore, SUMF2 mRNA levels negatively correlated with FBXW7 mRNA levels in BLCA. …”
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Evolution of the RNA alternative decay cis element into a high-affinity target for the immunomodulatory protein Roquin by Jan-Niklas Tants, Katharina Friedrich, Jasmina Neumann, Andreas Schlundt

“…Roquin initiates mRNA decay and contributes to balanced transcript levels required for immune homoeostasis. …”
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Supplementation of dairy cows with bovine somatotropin or omega-3 rich fish oil affects the endometrial expression of peroxisome proliferator-activated receptors by Leslie A. MacLaren, Todd R. Bilby, Frank Michel, Aydin Guzeloglu, Charles R. Staples, William W. Thatcher

“…Fish oil supplementation is associated with reduced PPAR&#948; mRNA abundance, but did not affect steady-state PPAR&#945; mRNA abundance. …”
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巨噬细胞ABCA1对Ox-LDL诱导的炎症因子的调节及其意义 by 郭志刚, 吴平生, 李建华, 赖文岩

“…【目的】研究在氧化低密度脂蛋白(Ox-LDL)诱导作用下,ATP结合盒转运子A1(ABCA1)对巨噬细胞中细胞间黏附分子-1(ICAM-1)、单核细胞化学趋向蛋白-1(MCP-1)mRNA和蛋白质及白介素-1β(IL-1β)蛋白质表达的影响,在细胞水平证实ABCA1对动脉粥样硬化的影响。…”
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Therapeutic Potential of Liraglutide for Diabetes–Periodontitis Comorbidity: Killing Two Birds with One Stone by Man Yang, Yunqing Pang, Minyu Pei, Yuanyuan Li, Xuemin Yuan, Rongbing Tang, Jing Wang

“…In addition, after treatment with LIRA, the ratio of RANKL/OPG was reduced, and the expression levels of ALP mRNA and Runx2 mRNA were upregulated (P<0.05). Conclusions. …”
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