Development of a 3-MicroRNA Signature and Nomogram for Predicting the Survival of Patients with Uveal Melanoma Based on TCGA and GEO Databases by Jun Zuo, Hongquan Ye, Jing Tang, Jianqun Lu, Qi Wan

“…The decision curve of nomogram revealed a good clinical usefulness of our nomogram. What’s more, a miRNA-mRNA network was constructed. Pathway enrichment showed that this network was largely involved in mRNA processing, the mRNA surveillance pathway, the spliceosome, and so on. …”
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甲基转移酶3对人脑血管平滑肌细胞基因表达及增殖和迁移功能的影响 Effects of Methyltransferase 3 on Gene Expression, Proliferation, and Migration Functions of Human Brain Vascular Smooth Muscle Cells... by 孟晨曦1，孙洪英2，张佳2，毛戬2，杨阳3，策乐木格3 (MENG Chenxi1, SUN Hongying2, ZHANG Jia2, MAO Jian2, YANG Yang3, Celemuge3 )

“…结果 与空白组相比，对照组细胞各基因的mRNA相对表达水平未发生明显改变。与对照组相比，干扰组细胞ZNF395（P=0.02）、ZNF548（P=0.03）、DIS3L（P=0.02）和GPR107（P＜0.01）的mRNA相对表达水平，以及ZNF395（P＜0.01）和ZNF548（P＜0.01）的m6A修饰水平均降低。…”
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Role of p57KIP2 in Stem and Progenitor Leydig Cells of Mouse Testes by Seung Hyun Park, Kyung Noh Yoon, Yang Xu, Myung Chan Gye

“…In THY1(+) isolated SLCs, p57kip2 siRNA significantly increased ki67 and pcna mRNA and pdgfrα mRNA, a differentiation marker and decreased nestin mRNA, a SLC marker. …”

Biphasic Modulation of NOS Expression, Protein and Nitrite Products by Hydroxocobalamin Underlies Its Protective Effect in Endotoxemic Shock: Downstream Regulation of COX-2, IL-1β,... by André L. F. Sampaio, Jesmond Dalli, Vincenzo Brancaleone, Fulvio D'Acquisto, Mauro Perretti, Carmen Wheatley

“…During the immune response, pro-inflammatory phase, parenteral hydroxocobalamin (HOCbl) treatment partially inhibits hepatic, but not lung, iNOS mRNA and promotes lung eNOS mRNA, but attenuates the LPS hepatic rise in eNOS mRNA, whilst paradoxically promoting high iNOS/eNOS protein translation, but relatively moderate •NO production. …”
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甲状腺相关眼病患者眼眶脂肪抵抗素的表达 by 刘红霞, 吴中耀, 杨华胜, 颜建华, 毛羽翔, 王莉菲, 林征

“…用RT-PCR法检测眶脂肪组织、前脂肪细胞及成熟脂肪细胞中抵抗素mRNA的表达，并进行免疫组化检测抵抗素蛋白的表达。【结果】前脂肪细胞中无抵抗素mRNA及蛋白的表达。…”
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Identification of Type VI Collagen Synthesizing Cells in Human Diabetic Glomerulosclerosis Using Renal Biopsy Sections by Mohammed Shawkat Razzaque, Takehiko Koji, Takashi Harada, Takashi Taguchi

“…For cellular localization of type VI collagen mRNA, paraffin‐embedded renal sections of the control and DN were hybridized in situ with digoxigenin (Dig)‐labeled antisense oligo‐DNA probe complementary to a part of \alpha1 (VI) mRNA. …”
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LncRNA与凋亡相关基因在小鼠肠缺血再灌后肠黏膜表达谱中的变化及共表达网络关系 by 许淼, 杨勇, 邓绮文, 沈建通, 刘卫锋, 杨文静, 刘克玄

“…【方法】采用lncRNA芯片高通量筛选小鼠肠I/R损伤早期肠黏膜中差异lncRNA的变化并用qRT-PCR验证芯片重复性，通过对与lncRNA同时差异表达的mRNA进行基因本体论（GO）富集分析，重点分析凋亡相关功能。最后，挑选差异变化的已知凋亡相关基因的mRNA，通过CNC分析计算出与之显著相关的IncRNA-mRNA关系对，构建差异lncRNA和凋亡mRNA共表达网络。…”
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The Correlation of PPARα Activity and Cardiomyocyte Metabolism and Structure in Idiopathic Dilated Cardiomyopathy during Heart Failure Progression by E. Czarnowska, D. Domal-Kwiatkowska, E. Reichman-Warmusz, J. B. Bierla, A. Sowinska, A. Ratajska, K. Goral-Radziszewska, R. Wojnicz

“…There were analogous changes in the expression of FAT/CD36 and CPT-1 mRNA in contrast to continuous overexpression of GLUT-4 mRNA and significant increase of PDK-4 mRNA in Group (II). …”
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Effect of long term heat stress and dietary restriction on the expression of small heat shock protein (shsp) genes in rat liver tissue by Faruk Bozkaya, Mehmet Osman Atli, Aydın Guzeloglu, Seyit Ali Kayis, Mehmet Salih Kaya, Nurettin Aydilek

“…<b>Aim:</b> Investigation of the effects of dietary restriction on expression of certain small heat shock protein (sHSP) genes at mRNA level in liver tissue of rats reared under long-term heat stress.…”
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Alterations of Dopamine-Related Transcripts in A11 Diencephalospinal Pathways after Spinal Cord Injury by Shunyi Zhao, Jaclyn H. DeFinis, Shaoping Hou

“…After 3 or 8 weeks, rostral (T5) and caudal (L1) spinal cord tissue was collected to measure mRNA levels of DA-related genes. Meanwhile, A11 neurons in the brain were explicitly isolated by laser capture microdissection, and single-cell qPCR was employed to evaluate mRNA levels in the soma. …”
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ERK5 Contributes to VEGF Alteration in Diabetic Retinopathy by Yuexiu Wu, Yufeng Zuo, Rana Chakrabarti, Biao Feng, Shali Chen, Subrata Chakrabarti

“…On the other hand, constitutively active MEK5, an activator of ERK5, increased ERK5 activation and ERK5 and KLF2 mRNA expression and attenuated basal- and glucose-induced VEGF mRNA expression. …”
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Cyclosporine A causes gingival overgrowth via reduced G1 cell cycle arrest in gingival fibroblasts. by Reiri Takeuchi, Takatoshi Nomura, Manabu Yaguchi, Noriko Kuwahara, Yuta Amino, Chieko Taguchi, Itaru Suzuki, Haruka Suzuki, Teruaki Nagashima, Kazumune Arikawa, Yuichiro Okada, Takato Nomoto, Koichi Hiratsuka

“…Treatment with cyclosporine A also increased MYC and ATM mRNA expression levels and decreased CDK2, ATR, P27, P53 and RB1 mRNA expression levels but not significantly. …”
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人类水通道蛋白在不同径线卵泡的黄素化颗粒细胞中的表达水平 by 郑泽桐, 谈际范, 李宇彬, 张秀冰, 刘思敏, 周灿权

“…结论AQP2在黄素化颗粒细胞中的mRNA水平随着卵泡径线的增长而增加，其表达量与促排卵方案有关，提示AQP2在卵泡生长和卵泡液生成过程中可能起到一定作用，且其mRNA表达水平可能受到FSH和LH的调节。…”
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Mechanical Stress-Induced IGF-1 Facilitates col-I and col-III Synthesis via the IGF-1R/AKT/mTORC1 Signaling Pathway by Bin Yan, Canjun Zeng, Yuhui Chen, Minjun Huang, Na Yao, Jie Zhang, Bo Yan, Jiajun Tang, Liang Wang, Zhongmin Zhang

“…Interestingly, col-I protein, col-I mRNA, col-III protein, col-III mRNA, and IGF-1 protein, but not IGF-1 mRNA, were inhibited by IGF-1 neutralizing antibody. …”
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核因子-κB对TNF-α诱导的脂肪细胞葡萄糖转运 蛋白4表达的影响

“…各组干预48 h后检测phospho- NF-κB p65(Ser536)的蛋白水平。GLUT4 mRNA及蛋白水平。蛋白检测采用Western法,mRNA检测采用RT-PCR法。【结果】 B组phospho- NF-κB p65蛋白水平(71.1 ± 5.9)高于A组(41.3 ± 1.7, P < 0.001)和C组(25.4 ± 4.7, P < 0.001)。…”
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3-Acetyldeoxynivalenol induces pyroptosis in leydig cells via METTL3-mediated N6-methyladenosine modification of NLRP3 by Yangyun Wang, Chaoliang Shi, Wei Jiao, Xiaoxiang Wan

“…Moreover, 3-ADON treatment resulted in a significant upregulation of METTL3 expression and increased global mRNA m6A modification levels. m6A sequencing and functional assays established that METTL3-mediated m6A modification of NLRP3 mRNA enhances its stability and expression. …”
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Expression of IGF-1R in oral submucous fibrosis and oral squamous cell carcinoma – An RT-PCR study by Vivek Nayyar, Vinay Hazarey, Sindhu M. Ganvir, Hemant J. Purohit, Reshma Talkal, Kiran Jot

“…Thus, we compared the expression of IGF-1R mRNA in normal oral mucosa (NOM), OSMF, and OSCC. …”
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Adiponectin Inhibits LPS-Induced HMGB1 Release through an AMP Kinase and Heme Oxygenase-1-Dependent Pathway in RAW 264 Macrophage Cells by Mohamed Elfeky, Ryuji Kaede, Yuko Okamatsu-Ogura, Kazuhiro Kimura

“…Adiponectin treatment decreased toll-like receptor 4 (TLR4) mRNA expression and increased heme oxygenase- (HO-) 1 mRNA expression without inducing IL-10 mRNA, while IL-10 treatment decreased TLR2 and HMGB1 mRNA expression and increased the expression of IL-10 and HO-1 mRNA. …”
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Data analysis of high-throughput sequencing and microarray to identify key signatures of microribonucleic acids in glioblastoma by A. A. Pushkin, E. A. Dzenkova, N. N. Timoshkina, D. Yu. Gvaldin

“…Networks of mRNA-miRNA interactions were built and expression profiles of genes and miRNAs characteristic of glioblastomas were developed. …”
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NLRP3炎症小体在甲型流感病毒H1N1预感染降低小鼠巨噬细胞抗MRSA免疫中的作用 by 师小函, 石云锋, 巴俊慧, 罗进梅, 胡佳佳, 吴本权

“…结果MRSA组NLRP3、Caspase-1的mRNA表达水平与空白对照组比无统计学差异（<italic>P</italic>均&gt;0.05），NLRP3的蛋白表达水平与空白对照组比无统计学差异（<italic>P</italic>&gt;0.05），MRSA组IL-1β的mRNA表达及上清液浓度均明显高于空白对照组（<italic>P</italic>均&lt;0.01）。…”
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