RT-PCR 分析nm23 基因在乳腺肿瘤中的转录表达 by 易基群, 杨晓明

“…【目的】探讨nm23 基因m RNA 在乳腺癌组织、乳腺良性肿瘤组织、癌旁组织中的表达及其与临床的关系。【方 法】采用半定量RT-PCR 方法, 检测30 例乳腺癌组织、8 例配对癌旁组织、9 例慢性纤维囊性乳腺病和6 例乳腺纤维腺瘤组织 nm23H1 和nm23H2 mRNA 的表达。…”
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Integrative Bioinformatics Analysis Unravel the Association between Intracerebral Hemorrhage and Cellular Apoptosis through Immune Infiltration by Pandi Chen, Gengfan Ye, Jia Li, Kuan Feng, Guangyao Zhu, Maosong Chen, Wei Chen

“…Key genes were identified through least absolute shrinkage and selection operator regression after model validation. Subsequently, mRNA-transcription factor (mRNA-TF), mRNA–microRNA (mRNA–miRNA), mRNA-RNA-binding protein (mRNA-RBP), and mRNA-drug regulatory networks were established, followed by immune infiltration analysis. …”
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一氧化氮在舌鳞癌血管生成中的作用 by 曾曙光

“…【结果】①舌鳞癌iNOS mRNA 表达阳性组微血管密度 显著高于阴性组(P <0.001);在iNOS mRNA 表达阳性组, iNOS mRNA 阳性表达水平与MVD 呈高度显著性正相关, r =0.6 (P <0.001)。…”
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Sex differences of molecular mechanisms of insulin sensitivity in young and adult C57BL/6J mice by T. V. Yakovleva, A. Yu. Kazantseva, E. N.  Makarova, N. M. Bazhan

“…It was shown that there are sex differences in the levels of mRNA Pik3cd in the liver, in the levels of mRNA Irs1 in the muscle, in the levels of mRNA Irs1 and Slc2a4 in adipose tissue at the age of 10 weeks, and there are sex differences in the levels of mRNA Irs2 and Pik3cd in the liver, in the levels mRNA Pik3cd and Slc2a4 in the muscle, in the levels mRNA Insr and Pik3cd in adipose tissue at the age of 30 weeks. …”
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Integrated analysis of miRNAs and mRNAs in thousands of single cells by Jia Li, Jing Tian, Tao Cai

“…Here, we developed a parallel single-cell small RNA and mRNA coprofiling method (PSCSR-seq V2) that enables miRNA and mRNA coexpression analysis in many cells. …”
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甲状腺乳头状癌雌激素受体β表达及其 5′非翻译区甲基化 by 苟茜, 许琳婉, 刘智敏

“…【方法】应用 Western blot 和免疫组织化学染色法检测正常甲状腺细胞Nthy-ori3-1、PTC 细胞 BCPAP 以及正常甲状腺组织、甲状腺结节性增生组织和 PTC 组织ERβ蛋白表达；应用 qRT-PCR 检测各细胞和组织中 ERβ mRNA（0K-1）、ERβ mRNA（0N-1）和总 ERβ mRNA 表达水平；亚硫酸氢盐测序分析各细胞和组织中 ERβ 基因启动子 0K、启动子 0N 和外显子 0N 的 CpG 岛甲基化状态；DNA 甲基化转移酶抑制剂5-aza-2′-deoxycytidine（5-aza-dC）处理 Nthy-ori3-1 细胞和 BCPAP 细胞后，qRT-PCR 检测 ERβ mRNA（0K-1）、ERβ mRNA（0N-1）和总 ERβ mRNA 表达水平。…”
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转录因子GATA家族在人血管内皮细胞中的表达

“…【目的】 了解转录因子GATA家族在人血管内皮细胞中表达的情况65377;【方法】 体外培养人的脐静脉内皮细胞(HUVECs)65380;主动脉内皮细胞(HAECs)65380;肺动脉内皮细胞(HPAECs)及肠系膜动脉内皮细胞(HMAECs) 4种血管内皮细胞,取1×106个上述细胞采用RT*9鄄PCR法检测转录因子GATA家族mRNA在人的上述四种血管内皮细胞中的表达情况并对结果进行定性分析65377;【结果】 GATA*9鄄2 mRNA 及GATA*9鄄3 mRNA在上述4种血管内皮细胞中均表达,GATA*9鄄2 mRNA 及GATA*9鄄3 mRNA在大部分人血管内皮细胞中表达,仅HUVECs细胞中未能检测到GATA*9鄄4的表达,HMAECs未能检测到GATA*9鄄6的表达65377;而在HUVEC65380;HAEC65380;HPAECs及HMAECs 4种血管内皮细胞均未检测到GATA*9鄄1 mRNA 及GATA*9鄄5 mRNA的表达65377;【结论】 转录因子GATA家族在人不同血管内皮细胞中的表达是不一致的,GATA*9鄄2 及GATA*9鄄3在上述4种血管内皮细胞中均表达,它们可能参与血管内皮细胞的基因调控65377;…”
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Spatiotemporal control of translation in live zebrafish embryos via photoprotected mRNAs by Florian P. Weissenboeck, Melissa Pieper, Helena Schepers, Sophie Hötte, Nils Klöcker, Sabine Hüwel, Andreas van Impel, Stefan Schulte-Merker, Andrea Rentmeister

“…Several mechanisms acting on the mRNA level regulate when and where an mRNA is expressed. …”
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正常妊娠和子痫前期胎盘Syncytin 表达差异探讨

“…【目的】 探讨syncytin及其受体ASCT2在子痫前期(PE)和正常妊娠胎盘组织中的表达改变65377; 【方法】 纳入研究病人共37例,分为2组,PE组21例,正常对照组16例,采用定量RT-PCR方法检测syncytin及ASCT2 mRNA的转录水平,Western blot法测定syncytin 蛋白表达强度,比较两组较检测结果65377; 【结果】 两组均检测到syncytin mRNA65380;syncytin蛋白及ASCT2 mRNA,PE组胎盘syncytin mRNA水平(1.5 ± 1.4)和蛋白表达强度(55.7 ± 26.1)明显低于正常对照组(6.2 ± 3.0,92.2 ± 36.4,P < 0.01), ASCT2 mRNA水平无明显差异(P > 0.05)65377; 【结论】 胎盘组织syncytin表达下调与PE发病密切相关,其受体ASCT2与PE发生无相关性65377;…”
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Comparison of the Pharmacological Effects of Paricalcitol and Doxercalciferol on the Factors Involved in Mineral Homeostasis by J. Ruth Wu-Wong, Masaki Nakane, Gerard D. Gagne, Kristin A. Brooks, William T. Noonan

“…In pig parathyroid cells, paricalcitol and the active form of doxercalciferol induced VDR translocation from the cytoplasm into the nucleus, suppressed PTH mRNA expression and inhibited cell proliferation in a similar manner, although paricalcitol induced the expression of CaSR mRNA more effectively. …”
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Ribosomal profiling as a tool for studying translation in plants: main results, problems and future prospects by D. A. Afonnikov, O. I. Sinitsyna, T. S. Golubeva, N. A. Shmakov, A. V. Kochetov

“…The expression of eukaryotic genes can be regulated at several stages, including the translation of mRNA. It is known that the structure of mRNA can affect both the efficiency of interaction with the translation apparatus in general and the choice of translation initiation sites. …”
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In Vitro Study on the Regulation of Annexin IV and VEGF by hCG in the Human Endometrium by Shaoyuan Xu, Jie Li, Xiaoyan Chen, Beiyu Liu

“…Compared with the control group, VEGF mRNA and protein were increased in the hCG group (mRNA/protein: 2.30-fold/1.86-fold), but not in the hCG/PD98059 group. …”
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PI3-K与活动性狼疮肾炎Th2 细胞因子的关系 by 王俭勤, 李幼姬, 李志坚, 张涤华, 周道远, 许韩师, 叶任高

“…【方法】利用免疫沉淀、免疫印迹和RT-PCR 技术检测12 例正常人和14 例活动性狼疮肾炎患者外周血单个 核细胞PI3-K 磷酸化产物、IL-6 mRNA 和IL-10 m RNA 表达, 观察PI 3-K 特异性抑制剂PY294002 对活动性狼疮肾炎患者外 周血单个核细胞IL-6 m RNA 和IL-10 m RNA 表达的影响。…”
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实时荧光定量PCR检测肾透明细胞癌hIRS-1和hIRS-2的表达及临床意义

“…【目的】 本研究检测hIRS-1 mRNA和hIRS-2 mRNA在肾透明细胞癌组织和对应癌旁正常组织中的表达,分析其与临床病理参数之间的关系,评价hIRS-1和hIRS-2的临床意义65377;【方法】 应用实时荧光定量PCR法对hIRS-1及hIRS-2基因在43例肾透明细胞癌组织和对应癌旁正常组织中的表达进行检测,分析其与临床病理指标的关系65377;【结果】 hIRS-1 mRNA在肾透明细胞癌和癌旁组织中均存在表达,hIRS-1基因在肾透明细胞癌组织和对应癌旁正常组织中比较无显著差异(0.612 ± 0.127 vs 0.645 ± 0.116,P > 0.05);而hIRS-2基因在肾透明细胞癌中存在高表达,hIRS-2基因在肾透明细胞癌组织和对应癌旁正常组织中比较有显著差异(1.016 ± 0.143 vs 0.792 ± 0.136,P < 0.05);hIRS-1 mRNA的表达与患者年龄65380;性别65380;肿瘤大小65380;病理分级及临床分期无关(P > 0.05);hIRS-2 mRNA的表达与肿瘤大小及临床分期有关(P < 0.05),与患者年龄65380;性别65380;及病理分级无关(P > 0.05)65377;【结论】 hIRS-1 mRNA在肾透明细胞癌和癌旁组织中均存在表达,两者比较差异无统计学意义;hIRS-2 mRNA在肾透明细胞癌中存在高表达,提示hIRS-2基因在肾透明细胞癌细胞癌变过程中可能发挥重要作用65377; 关键词: 肾透明细胞癌; 实时荧光定量PCR; 胰岛素受体底物…”
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灵孢多糖对脂多糖诱导的原代多巴胺能神经元变性的保护作用 by 杨海华, 徐评议, 刘焯霖, 朱雯, 李毅, 叶钦勇

“…脂多糖组激活的小胶质细胞体积增大，细胞数量增多（30．6±4．5），TNF-α mRNA和iNOS mRNA表达增高，但经较大剂量灵孢多糖（100，300μg／mL）预处理后，小胶质细胞的激活被抑制，总的细胞数量显著减少（26．4±5．1，25．1±4．6），TNF-α mRNA和iNOS mRNA表达量降低（P〈0．01）。…”
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Expression of dna methyltransferases (dnmts) at mrna level in ovine endometrium during estrus cycle and early pregnancy by Mustafa Hitit, Mehmet Kose

“…<b>Aim: </b>To elucidate mRNA expression of DNA methyltransferase enzyme genes (DNMT1, DNMT3A, and DNMT3B) in ovine endometrium whether they are modulated during the estrus cycle and early pregnancy.…”
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Biosynthetic pathway for leukotrienes is stimulated by lipopolysaccharide and cytokines in pig endometrial stromal cells by Barbara Jana, Aneta Andronowska, Jarosław Całka, Aleksandra Mówińska

“…LPS increased LTAH mRNA abundance, while TNF-α, IL-1β and IL-10 augmented LTAH mRNA and protein abundances. …”
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缬沙坦对U937泡沫细胞血管内皮生长因子表达与分泌的影响

“…【目的】 观察缬沙坦对泡沫细胞血管内皮生长因子(VEGF)mRNA表达与蛋白分泌的影响?【方法】 以氧化型低密度脂蛋白刺激体外培养的人单核细胞株U937细胞,建立U937泡沫细胞模型?…”
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Altered Protein Structures and Neoepitopes in Lupus Neutrophils From Dysregulated Splicing of Messenger RNA by Rayan Najjar, Xiaoxing Wang, Jose Mario Bello Pineda, Hugh Alessi, Alison Bays, Robert K. Bradley, James N. Jarvis, Tomas Mustelin

“…Objective To test whether messenger RNA (mRNA) splicing is altered in neutrophils from patients with systemic lupus erythematosus (SLE) and can produce neoantigens. …”
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外周血单个核细胞caveolin-1的表达在新诊断2型糖尿病患者中的变化及其与胰岛素抵抗的关系

“…【方法】 检测47例新诊断2型糖尿病患者和41例正常血糖对照者PBMC中caveolin-1 mRNA和蛋白的表达,分析caveolin-1 mRNA表达与体质指数(BMI)、腰围、臀围、腰臀比、空腹血糖(FPG)、糖化血红蛋白(HbA1C)、空腹胰岛素(FINS)、稳态模型的胰岛素抵抗指数(HOMA-IR)和血脂的相关性。…”
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