Long Noncoding RNA–Messenger RNA (lncRNA–mRNA) Network in Resistance to Gummy Stem Blight (<i>Stagonosporopsis cucurbitacearum</i>) of Melon Plant Introduction (PI) 420145 by Rui Zhang, Jing Chen, Yongbing Zhang, Xiumei Lu, Chunyan Cheng, Ji Li, Qunfeng Lou, Yuhui Wang, Chuntao Qian

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Distinct response patterns of endothelial markers to the BNT162b2 mRNA COVID-19 booster vaccine are associated with the spike-specific IgG antibody production by Beatriz Castro-Robles, Francisco J. Cimas, Francisco J. Cimas, Lourdes Arias-Salazar, Jesús Ontañón, Julia Lozano, Susana López-López, Fernando Andrés-Pretel, María Ángeles Requena-Calleja, Antonio Mas, Antonio Mas, Antonio Mas, Gemma Serrano-Heras, Tomás Segura, Tomás Segura, Javier Solera

Subjects: “…mRNA COVID-19 vaccines…”
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Comparative mRNA Expression of eEF1A Isoforms and a PI3K/Akt/mTOR Pathway in a Cellular Model of Parkinson’s Disease by Kawinthra Khwanraj, Suriyat Madlah, Khwanthana Grataitong, Permphan Dharmasaroja

“…In this paper, the expression of all gene members of the PI3K/Akt/mTOR pathway in relation to those of the eEF1A isoforms in a cellular model of PD was investigated at the mRNA level. The results showed a similar trend of upregulation of genes of the eEF1A isoforms (eEF1A1 and eEF1A2) and of the PI3K (classes I–III)/Akt (Akt1, Akt2, and Akt3)/mTOR (mTORC1 and mTORC2) pathway in both nondifferentiated and differentiated SH-SY5Y dopaminergic cells treated with 1-methyl-4-phenylpyridinium (MPP+). …”
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Jet Injection of Naked mRNA Encoding the RBD of the SARS-CoV-2 Spike Protein Induces a High Level of a Specific Immune Response in Mice by Denis N. Kisakov, Larisa I. Karpenko, Lyubov A. Kisakova, Sergey V. Sharabrin, Mariya B. Borgoyakova, Ekaterina V. Starostina, Oleg S. Taranov, Elena K. Ivleva, Oleg V. Pyankov, Anna V. Zaykovskaya, Elena V. Dmitrienko, Vladimir A. Yakovlev, Elena V. Tigeeva, Irina Alekseevna Bauer, Svetlana I. Krasnikova, Nadezhda B. Rudometova, Andrey P. Rudometov, Artemiy A. Sergeev, Alexander A. Ilyichev

Subjects: “…mRNA vaccines…”
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HPV E6/E7, hTERT, and Ki67 mRNA RT-qPCR Assay for Detecting High-Grade Cervical Lesion with Microscope Slides by Geehyuk Kim, Jemberu Taye, Kwangmin Yu, Sunyoung Park, Jungho Kim, Sunghyun Kim, Dongsup Lee, Hye-young Wang, Kwang Hwa Park, Hyeyoung Lee

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Regulation of Paneth cell-specific genes in COVID-19 patients and SARS-CoV-2-infected mice by quantification of mRNA from exfoliated cells in stool samples by Gill Diamond, Erika L. Figgins, Denny Gao, Tanya O. Robinson, Kenneth Wilson, Jon D. Gabbard, Charles D. Anderson, Lalit Batra, Chithra Sreenivasan, Jian Zheng, Uyen Do, Braden Mulcahy, Lisa K. Ryan, Nathaniel McLaughlin, Anna H. Owings, Neha Dhaliwal, Haley B. Williams, T. Grant Wichman, Michelle Galeas-Pena, Sarah C. Glover

“…Results revealed a significant decrease in mRNA levels from Paneth cell-specific genes, including DEFA5, DEFA6, PLA2G2A, PRSS2 and ITLN2 in SARS-CoV-2 positive patients compared with controls. …”
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美托洛尔对心力衰竭大鼠心脏β3肾上腺素能受体 mRNA 表达水平的影响

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Regulating Immune Responses Induced by PEGylated Messenger RNA–Lipid Nanoparticle Vaccine by Hyein Jo, Jaewhoon Jeoung, Wonho Kim, Dooil Jeoung

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PIWI proteins and piRNAs: key regulators of stem cell biology by Fernando Claro-Linares, Patricia Rojas-Ríos

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Patterns of Isoform Variation for N Gene Subgenomic mRNAs in Betacoronavirus Transcriptomes by James J. Kelley, Andrey Grigoriev

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Comprehensive bioinformatics analysis identifies hub genes associated with immune cell infiltration in early-onset schizophrenia by Shasha Wu, Tailian Xue, Yilin Li, Weikang Chen, Yan Ren

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Exploring the CD3/CD56/TNF-α/Caspase3 pathway in pyrethroid-induced immune dysregulation: curcumin-loaded chitosan nanoparticle intervention by Nawal Alsubaie, Yasmina M. Abd-Elhakim, Amany Abdel-Rahman Mohamed, Tarek Khamis, Tarek Khamis, Mohamed M. M. Metwally, Mohamed M. M. Metwally, Nawal Helmi, Afnan M. Alnajeebi, Badriyah S. Alotaibi, Amirah Albaqami, Wedad Mawkili, Mai A. Samak, Mai A. Samak, Samar A. Eissa

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SP-1-activated LINC01016 overexpression promotes gastric cancer invasion and metastasis through inhibiting EIF4A3-mediated MMP9 mRNA decay by Ying Sun, Hui Zhang, Duan-bo Shi, Peng Gao

“…We determined that LINC01016 can blocks the binding of EIF4A3 to MMP9 mRNA, thereby inhibiting EIF4A3-mediated nonsense-mediated RNA decay (NMD), increasing MMP9 mRNA level and protein expression levels to promote tumor progression. …”
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吉西他滨对人肺癌A549细胞株CN-Ⅱ, APE/Ref-1 mRNA 和蛋白表达的影响

“…【目的】 研究人肺癌A549细胞株在吉西他滨化疗时CN-Ⅱ, APE/Ref-1基因表达的变化,并探讨其在肺癌化疗耐药中所起的作用65377;【方法】 不同浓度吉西他滨065380;1065380;2065380;40及60 μmol/L作用人肺癌A549细胞株24 h,分别以RT-PCR及Western blot方法测定用药后CN-Ⅱ和APE/Ref-1的mRNA及蛋白表达情况65377; 【结果】 吉西他滨作用人肺癌A549细胞株24 h后, CN-Ⅱ和APE/Ref-1的mRNA及蛋白表达水平均明显上升,并与吉西他滨的浓度呈正相关(CN-Ⅱ RT-PCR: r = 0.687, P = 0.009; Western blot: r = 0.594, P = 0.021; APE/Ref-1 RT-PCR: r = 0.669, P = 0.010; Western blot: r = 0.562, P = 0.029)65377; 【结论】 CN-Ⅱ和APE/Ref-1在肺癌化疗时表达明显增强,可能与化疗耐药性的产生有关,并提示针对CN-Ⅱ和APE/Ref-1的靶向干预可能有助于提高肺癌的化疗敏感性65377;…”
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Expression Profiles of Differentially Expressed Circular RNAs and circRNA–miRNA–mRNA Regulatory Networks in SH-SY5Y Cells Infected with Coxsackievirus B5 by Jing Li, Heng Yang, Huaran Shi, Jihong Zhang, Wei Chen

“…Additionally, RT-qPCR was used to validate the RNA-seq data, and a circRNA–miRNA–mRNA interaction network was constructed based on two circRNAs, such as hsa_circ_0008378 and novel_circ_0014617, which were associated with the regulation of innate immune response in host cells. …”
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LIN28A-dependent lncRNA NEAT1 aggravates sepsis-induced acute respiratory distress syndrome through destabilizing ACE2 mRNA by RNA methylation by Jun Liu, Xiang Li, Peng Yang, Yufeng He, Weilong Hong, Yawei Feng, Zhiqiang Ye

“…Besides, NEAT1 destabilized ACE2 mRNA depending on RNA methylation by forming methylated NEAT1/hnRNPA2B1/ACE2 mRNA complex in LPS-treated AT-II cells. …”
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实时荧光定量 PCR检测野生型p53介导蛋白磷酸酶mRNA 在非小细胞肺癌中的表达

“…【目的】 荧光染料Ⅰ(SYBR GreenⅠ)是一种较为常用的非探针类定量PCR的方法,其结果具有一定特异性65377;本研究的目的是建立检测野生型p53介导蛋白磷酸酶(Wip1)mRNA的SYBR GreenⅠ实时定量PCR的方法,了解肺癌组织及其远癌正常肺组织中Wip1的表达水平,研究肺癌组织中Wip1的表达水平与各种临床病理特征之间的关系65377; 【方法】 利用相对定量的方法检测44例肺癌及相应远癌正常肺组织中Wip1 mRNA相对于β-actin mRNA的表达量,进行相对定量分析65377; 【结果】 44例非小细胞肺癌(NSCLC)及相应远癌正常肺组织均有Wip1 mRNA的表达,其中17例非小细胞肺癌中Wip1 mRNA高表达,占38.6%,两者表达差异有统计学意义 (Ratio = 2.1644 ± 1.394,P < 0.05);分化程度低的肺癌组织中Wip1 mRNA表达量显著高于分化程度高者(F = 5.08,P = 0.013)65377; 【结论】 SYBR GreenⅠ实时定量PCR方法可以成功地检测Wip1基因的表达量65377;初步检测结果显示Wip1可能是一种肺癌发生发展过程中的致癌因素65377;…”
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Relationship between Fecal Content of Fatty Acids and Cyclooxygenase mRNA Expression and Fatty Acid Composition in Duodenal Biopsies, Serum Lipoproteins, and Dietary Fat in Colectomized Familial Adenomatous Polyposis Patients by K. Almendingen, A. T. Høstmark, L. N. Larsen, O. Fausa, J. Bratlie, L. Aabakken

“…We report here findings of an observational study on 29 colectomized familial adenomatous polyposis patients that describe the fecal content of fatty acids, and relate this to the proportions of fatty acids and levels of cyclooxygenase mRNA expression in duodenal biopsies, levels of serum lipoproteins, and diet. …”
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反义IL-6寡核苷酸对实验性系膜增生性肾炎IL-6,IL-6mRNA表达的影响

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Acute zonal occult outer retinopathy (AZOOR) complex associated with torpedo retinopathy following COVID-19 recombinant mRNA vaccination: a case report of a rare clinical presentation by Nai-Wen Chang, Chia-hao Kuo, Hung-Da Chou, Yi-Hsing Chen

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