Comprehensive analyses reveal the promising value of gasdermins as prognostic biomarkers and immunotherapeutic targets in head and neck squamous cell carcinoma by Huageng Huang, Jingjing Ge, Shunzhen Lu, Xinyi Deng, Ying Tian, He Huang, Zhao Wang, Yuyi Yao, Huangming Hong, Tongyu Lin

“…Compared with those in normal tissues, the mRNA and protein levels of GSDMs, especially GSDMD/E, were commonly elevated in HNSC (P < 0.05). …”
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HINT1 suppression protects against age-related cardiac dysfunction by enhancing mitochondrial biogenesis by Michio Sato, Tsuyoshi Kadomatsu, Jun Morinaga, Yuya Kinoshita, Daisuke Torigoe, Haruki Horiguchi, Sumio Ohtsuki, Shuji Yamamura, Ryoko Kusaba, Takanori Yamaguchi, Goro Yoshioka, Kimi Araki, Tomohiko Wakayama, Keishi Miyata, Koichi Node, Yuichi Oike

“…Furthermore, expression of the lncRNA Caren, which inhibits Hint1 mRNA translation, decreased with age in heart, and CR suppressed this effect. …”
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Comparative evaluation of ACetic - MEthanol high salt dissociation approach for single-cell transcriptomics of frozen human tissues by Marina Utkina, Anastasia Shcherbakova, Ruslan Deviatiiarov, Ruslan Deviatiiarov, Ruslan Deviatiiarov, Alina Ryabova, Marina Loguinova, Valentin Trofimov, Anna Kuznetsova, Mikhail Petropavlovskiy, Rustam Salimkhanov, Denis Maksimov, Eugene Albert, Alexandra Golubeva, Walaa Asaad, Lilia Urusova, Ekaterina Bondarenko, Anastasia Lapshina, Alexandra Shutova, Dmitry Beltsevich, Oleg Gusev, Oleg Gusev, Oleg Gusev, Oleg Gusev, Larisa Dzeranova, Galina Melnichenko, Ildar Minniakhmetov, Ivan Dedov, Natalya Mokrysheva, Sergey Popov

“…This technique aims to preserve cell morphology and RNA integrity, minimizing transcriptome changes and providing a more accurate representation of mature mRNA. We compared the ability of enzymatic, ACME HS, and nuclei isolation methods to preserve major cell types, gene expression, and standard quality parameters across 41 tissue samples. …”
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Xixin Decoction’s novel mechanism for alleviating Alzheimer’s disease cognitive dysfunction by modulating amyloid-β transport across the blood–brain barrier to reduce neuroinflamma... by Chaokai Yang, Enlong Zhao, Hu Zhang, Liqi Duan, Xinyue Han, Hongli Ding, Yan Cheng, Dengkun Wang, Dengkun Wang, Dengkun Wang, Xiaojing Lei, Xiaojing Lei, Xiaojing Lei, Yongchang Diwu, Yongchang Diwu, Yongchang Diwu

“…BBB integrity was evaluated using Evans blue staining, and protein expression levels were analyzed via ELISA, Western blotting, and immunofluorescence.ResultsIn vitro experiments revealed that XXD-containing serum, when cultured for 24, 48, and 72 h, could upregulate the expression of P-gp mRNA and protein, downregulate CB1 protein expression, and upregulate CB2 and Mfsd2a protein expression. …”
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Intervention effect and mechanism of Dracocephalum moldavica L. extract on bleomycin-induced pulmonary fibrosis in rats by Xiaoyu SUN, Li CHEN, Ruifang GAO, Hui YU, Min JIN

“…Real-time fluorescence quantitative PCR (RT-qPCR) was used to detect α-SMA and Col-I mRNA levels in lung tissue. Enzyme-linked immunosorbent assay (ELISA) was used to detect tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) in rats. …”
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Genetic characteristics involving the PD-1/PD-L1/L2 and CD73/A2aR axes and the immunosuppressive microenvironment in DLBCL by Tingting Zhang, Zhenzhen Zhang, Xiubao Ren, Jin He, Zhengzi Qian, Bin Meng, Lei Jiao, Hengqi Liu, Lanfang Li, Lihua Qiu, Shiyong Zhou, Wenchen Gong, Huilai Zhang, Xianhuo Wang

“…CD73 genetic mutations did not increase mRNA and protein expression. Patients with genetically altered CD73 tended to have a better overall survival than patients with wild-type CD73. …”
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Solid Predominant Histology and High Podoplanin Expression in Cancer-Associated Fibroblast Predict Primary Resistance to Osimertinib in EGFR-Mutated Lung Adenocarcinoma by Yuji Uehara, MD, Hiroki Izumi, MD, PhD, Tetsuro Taki, MD, PhD, Tetsuya Sakai, MD, PhD, Hibiki Udagawa, MD, PhD, Eri Sugiyama, MD, PhD, Shigeki Umemura, MD, PhD, Yoshitaka Zenke, MD, PhD, Shingo Matsumoto, MD, PhD, Kiyotaka Yoh, MD, PhD, Shoko Kubota, MD, PhD, Keiju Aokage, MD, PhD, Naoya Sakamoto, MD, PhD, Shingo Sakashita, MD, PhD, Motohiro Kojima, MD, PhD, Michiko Nagamine, MD, PhD, Yukio Hosomi, MD, PhD, Masahiro Tsuboi, MD, PhD, Koichi Goto, MD, PhD, Genichiro Ishii, MD, PhD

“…We also investigated HGF mRNA expression levels, using The Cancer Genome Atlas Program and Singapore Oncology Data Portal cohorts. …”
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In vitro functional validation of anti-CD19 chimeric antigen receptor T cells expressing lysine-specific demethylase 1 short hairpin RNA for the treatment of diffuse large B cell l... by Zhi Guo, Zhi Guo, Mingxin He, Ning Liu, Yiqing Yang, Rui Sun, Jianxun Wang, Qiang Wang

“…Epigenetic modifications can regulate T cell activation, maturation and depletion; they can be applied to reduce T cell depletion, improve infiltration, and promote memory phenotype formation to reduce relapse after CAR-T cell therapy.PurposeWe propose to develop and validate in vitro the function of novel CAR-T cells for the treatment of DLBCL, which simultaneously express an anti-CD19 CAR with lysine-specific demethylase 1 (LSD1) short hairpin (sh)RNA to prevent depletion and prolong the survival of CAR-T cells.MethodsWe designed an shRNA sequence targeting LSD1 mRNA, and created a vector with the following elements: the U6 promoter driving expression of the LSD1 shRNA sequence, the EF1a promoter driving a second-generation anti-CD19 CAR sequence encoding an anti-CD19 single-chain variable fragment (FMC63), the CD8 hinge and transmembrane structural domains, the CD28 co-stimulatory structural domain, and the CD3ζ-activating structural domain. …”
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gga-miR-6634-5p Affects the proliferation and steroid hormone secretion of chicken (Gallus Gallus) granulosa cells by targeting MMP16 by Yixiang Tian, Xing Wu, Zihao Zhang, Yijie Li, Meng Hou, Ruirui Jiang, Donghua Li, Yanhua Zhang, Yulong Guo, Guoxi Li, Xiaojun Liu, Xiangtao Kang, Yadong Tian, Wenting Li, Yuetao Li

“…We found that gga-miR-6634-5p significantly down-regulated the mRNA levels of proliferation-related genes (CCND1, CDK1, and CDK6), decreased cell viability, the number of EdU-labelled positive cells, and the percentage of S-phase cells, as analysed by quantitative real time PCR (qRT-PCR), cell counting kit-8 (CCK-8), 5-ethynyl-2′deoxyuridine (EdU) and flow cytometry analyses (P < 0.01 or P < 0.05). qRT-PCR and enzyme-linked immunosorbent assay (ELISA) results demonstrated that gga-miR-6634-5p up-regulated the expression of steroid synthesis-related genes (CYP19A1, 3β-HSD, StAR and FSHR) (P < 0.01 or P < 0.05), as well as the secretion of estradiol (E2) and progesterone (P4) (P < 0.01 or P < 0.05). …”
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Hyperthermia suppresses the biological characteristics and migration of chicken primordial germ cells by Yuzhou Gu, Yuzhou Gu, Yuzhou Gu, Kexin Wu, Kexin Wu, Kexin Wu, Bowen Niu, Bowen Niu, Bowen Niu, Zhiting Wang, Zhiting Wang, Zhiting Wang, Yuchen Jie, Yuchen Jie, Yuchen Jie, Zixuan Fan, Zixuan Fan, Zixuan Fan, Junying Li, Junying Li, Congjiao Sun, Congjiao Sun, Congjiao Sun, Zhuo-Cheng Hou, Zhuo-Cheng Hou, Zhuo-Cheng Hou, Li-Wa Shao, Li-Wa Shao, Li-Wa Shao

“…Cell viability, glycogen granule contents, and mRNA expression levels of pluripotency markers (NANOG and POUV), germ cell-specific markers (DAZL and CVH), and telomerase reverse transcriptase (TERT) were reduced in PGCs cultured under high temperatures (43°C for 12, 24, and 48 h). …”
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Molecular assessment of splicing variants in a cohort of patients with inborn errors of immunity: methodological approach and interpretation remarks by Laura Miguel Berenguel, Carla Gianelli, Carla Gianelli, Carla Gianelli, Elisabet Matas Pérez, Teresa del Rosal, Ana Méndez Echevarría, Ángel Robles Marhuenda, Marta Feito Rodríguez, Maria Teresa Caballero Molina, Maria Teresa Caballero Molina, Lorena Magallares García, Brenda Sánchez Garrido, Samantha Hita Díaz, Luis Allende Martínez, Luis Allende Martínez, Pilar Nozal Aranda, Pilar Nozal Aranda, Pilar Nozal Aranda, Carmen Cámara Hijón, Carmen Cámara Hijón, Carmen Cámara Hijón, Eduardo López Granados, Eduardo López Granados, Eduardo López Granados, Rebeca Rodríguez Pena, Rebeca Rodríguez Pena, Rebeca Rodríguez Pena, María Bravo García-Morato, María Bravo García-Morato, María Bravo García-Morato

“…BackgroundSplicing is the molecular mechanism to produce mature messenger RNA (mRNA) before its translation into protein. It is estimated that 50% of disease-causing mutations disrupt splicing, mostly of them affecting canonical positions. …”
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BPZ inhibits early mouse embryonic development by disrupting maternal-to-zygotic transition and mitochondrial function by Zhiming Ding, Huilei Chen, Huiru Cheng, Caiyun Wu, Hongzhen Ruan, Bingjing Zhu, Ping Zhou, Zuying Xu, Huifen Xiang

“…Mechanistically, BPZ exposure inhibits the activation of the zygotic genome and impedes maternal mRNA degradation, thereby interfering with maternal-to-zygotic transition (MZT). …”
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Biosynthesis inhibition of miR-142-5p in a N 6-methyladenosine-dependent manner induces neuropathic pain through CDK5/TRPV1 signaling by Jinshi Li, Yang Guo, Chen Zhu, Dongxu Wang, Yuan Li, Xiaotong Hao, Linyan Cao, Yiting Fan, Bo Fang

“…MiR-142-5p was shown to simultaneously bind to the mRNA of CDK5 and TRPV1, thereby inhibiting their expression. …”
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Dual specific STAT3/5 degraders effectively block acute myeloid leukemia and natural killer/T cell lymphoma by Daniel Pölöske, Helena Sorger, Anna Schönbichler, Elvin D. deAraujo, Heidi A. Neubauer, Anna Orlova, Sanna H. Timonen, Diaaeldin I. Abdallah, Aleksandr Ianevski, Heikki Kuusanmäki, Marta Surbek, Elisabeth Heyes, Thomas Eder, Christina Wagner, Tobias Suske, Martin L. Metzelder, Michael Bergmann, Maik Dahlhoff, Florian Grebien, Roman Fleck, Christine Pirker, Walter Berger, Emir Hadzijusufovic, Wolfgang R. Sperr, Lukas Kenner, Peter Valent, Tero Aittokallio, Marco Herling, Satu Mustjoki, Patrick T. Gunning, Richard Moriggl

“…JPX‐0700/−0750 decreased the mRNA and protein levels of STAT3/5 targets involved in cancer survival, metabolism, and cell cycle progression, exhibiting nanomolar to low micromolar efficacy. …”
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The association of single nucleotide polymorphisms in tumor necrosis factor 3 with susceptibility/resistance of Magallana gigas to Halomonas sp. 7T by Hongmei Fan, Qingyu Peng, Tianbao Liu, Lei Fang, Chuanyan Yang, Ning Kong, Lingling Wang, Linsheng Song

“…MgTNF-3 was constituted of a typical TNF domain and a transmembrane region, and shared low sequence identity and similarity with the TNF superfamily members from vertebrates and other invertebrates. The MgTNF-3 mRNA was constitutively expressed in haemocytes and all the examined tissues with the highest expression level in adductor muscle, and was significantly induced by lipopolysaccharide stimulation. …”
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Plural molecular and cellular mechanisms of pore domain KCNQ2 encephalopathy by Timothy J Abreo, Emma C Thompson, Anuraag Madabushi, Kristen L Park, Heun Soh, Nissi Varghese, Carlos G Vanoye, Kristen Springer, Jim Johnson, Scotty Sims, Zhigang Ji, Ana G Chavez, Miranda J Jankovic, Bereket Habte, Aamir R Zuberi, Cathleen M Lutz, Zhao Wang, Vaishnav Krishnan, Lisa Dudler, Stephanie Einsele-Scholz, Jeffrey L Noebels, Alfred L George, Atul Maheshwari, Anastasios Tzingounis, Edward C Cooper

“…G256W/+ pyramidal cell KCNQ2 and KCNQ3 immunolabeling was significantly shifted from axon initial segments to neuronal somata. Despite normal mRNA levels, G256W/+ mouse KCNQ2 protein levels were reduced by about 50%. …”
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Apheresis for the treatment of relapses in MS and NMOSD: reduced antibody reactivities, gene expression changes and potential clinical response indicators by Michael Hecker, Brit Fitzner, Isis Ludwig-Portugall, Friederike Bohne, Edmar Heyland, Juliane Klehmet, Matthias Grothe, Matthias Schwab, Alexander Winkelmann, Stefanie Meister, Ales Dudesek, Hannah Wurm, Ilya Ayzenberg, Ingo Kleiter, Ingo Kleiter, Corinna Trebst, Martin W. Hümmert, Bernhard Neumann, Bernhard Neumann, Klaus Eulitz, Dirk Koczan, Uwe K. Zettl

“…We then measured (1) concentrations of the immunoglobulin isotypes IgG, IgM and IgA, (2) antibody reactivities against 12 peptides derived from potential autoantigens and Epstein-Barr virus proteins, (3) frequencies of CD19+ B cells, CD3+ T cells and CD14+ monocytes, (4) transcriptome profiles of CD19+ B cells and CD4+ T cells and (5) mRNA levels of 7 cytotoxicity-related genes in CD4+ T cells. …”
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Effect of integrin α5 on NLRP3 expression in periodontal ligament fibroblasts within an inflammatory microenvironment by DAI Jingyi, CAI Hongxuan, SI Weixing, ZHANG Zan, WANG Zhurui, LI Mengsen, TIAN Ya guang

“…For <i>in vitro</i> experiments, compared to the 0-CM group, both the protein and mRNA levels of integrin α5 and NLRP3 were significantly increased in rat PDLFs in the 5-CM group (<i>P &lt; </i>0.05). …”
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Epithelial NSD2 maintains FMO‐mediated taurine biosynthesis to prevent intestinal barrier disruption by Yue Xu, Xiuying Xiao, Chunxiao Ma, Ziyi Wang, Wenxin Feng, Hanyu Rao, Wei Zhang, Ningyuan Liu, Rebiguli Aji, Xiangjun Meng, Wei‐Qiang Gao, Li Li

“…Mechanistically, NSD2 loss led to downregulation of H3K36me2 and flavin‐containing monooxygenase (FMO) (taurine‐synthesis enzyme) mRNA, resulting in decreased taurine biosynthesis in IECs. …”
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Sexual dimorphism in lung transcriptomic adaptations in fetal alcohol spectrum disorders by Vishal D. Naik, Dylan J. Millikin, Daniel Moussa, Hong Jiang, Alexander L. Carabulea, Joseph D. Janeski, Jiahui Ding, Kang Chen, Marta Rodriguez-Garcia, Sunil Jaiman, Stephen A. Krawetz, Gil Mor, Jayanth Ramadoss

“…Samples with RNA Integrity Numbers (RINs) > 8 were prepared using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB), xGen Broad-range RNA Library Prep (IDT), and xGen Normalase UDI Primer Plate 2 (IDT). …”
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