Transcriptome-wide dynamics of m6A methylation in ISKNV and Siniperca chuatsi cells infected with ISKNV by Qijin Miao, Jing Jiang, Siyou Huang, Jie Gao, Qingqing Liu, Rui Zheng, Yiling Kang, Changjun Guo, Jianguo He, Junfeng Xie

“…Co-expression analysis revealed that the genes differentially expressed at both the mRNA and m6A modification levels were enriched in pathways such as the hippo, ErbB, and JAK-STAT pathways. …”
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Prenatal light exposure affects diurnal rhythms and visual development of the layer embryonic retina by S.Q. Wei, P. Yin, W.Y. Tang, Z.Y. Zhang, W. Chu, Q. Tong, B.M. Li, W.C. Zheng, C.Y. Wang

“…In contrary, the expression level of TGF-β1 mRNA and Smad2/3 protein in retina was dramatically downregulated in WL when compared to that in NL and GL (P < 0.01). …”
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Recommendation for the use of respiratory syncytial virus vaccines by Ping-Ing Lee, Yhu-Chering Huang, Ching-Chuan Liu, Shee-Uan Chen, Po-Ren Hsueh, Shih-Chi Ku, Po-Yen Chen, Chih-Jung Chen, Yi-Tsung Lin, Chun-Yi Lu, Nan-Chang Chiu, Hsin Chi, Yee-Chun Chen, Feng-Yee Chang, Muh-Yong Yen, Chin-Te Lu, Kuang-Yao Yang, Cheng-Hsun Chiu, Kao-Pin Hwang, Wen-Sen Lee, Ting-Yu Yen, Jen-Fu Hsu, Ying-Chin Lin, Ya-Li Hu, Tzou-Yien Lin

“…RSV is also a significant viral pathogen for adults with respiratory tract infection.Vaccines targeting the pre-fusion protein of RSV, including recombinant and mRNA vaccines, are now available. A committee of experts from related fields was convened by the Taiwan Immunization Vision and Strategy to develop recommendations for RSV vaccination in the elderly and pregnant women.The recommendation is not intended as a sole source of guidance in the prevention of RSV infection in children. …”
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Knockout of <i>dhx38</i> Causes Inner Ear Developmental Defects in Zebrafish by Mengmeng Ren, Xiang Chen, Liyan Dai, Jiayi Tu, Hualei Hu, Xiaohan Sun, Jiong Luo, Pei Li, Yiyang Fu, Yuejie Zhu, Weiqiang Sun, Zhaohui Tang, Mugen Liu, Xiang Ren, Qunwei Lu

“…DHX38, a DEAH box RNA helicase, is integral to pre-mRNA splicing regulation and plays critical roles in development, cell differentiation, and stem cell maintenance. …”
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MMS21通过维持MCM2-7复合物稳定性促进肝细胞癌增殖活性 by 李文佳, 朱元昕, 胡开顺

“…方法利用GEPIA2和CPATC数据库分析MMS21在肝细胞癌中的mRNA和蛋白表达变化及与患者预后的关系；设计两条siRNA分别敲低肝癌细胞内源性MMS21的表达，并采用流式细胞技术检测细胞周期分布比例；进行BrdU染色和DNA fiber实验检测细胞增殖能力与DNA合成速度；构建带有MYC标签的MMS21蛋白过表达载体，并在细胞系SK-hep1中建立其稳定表达的细胞株；利用Co-IP联合质谱分析的方法鉴定MMS21的互作蛋白组并进行信号通路富集分析；采用Western Blot，检测MMS21对细胞周期相关蛋白的影响。…”
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Dose effects of iron on growth, antioxidant potential, intestinal morphology, and intestinal barrier in yellow-feathered broilers by J. Chen, K.W. Lei, S.Y. Li, D.P. Li, Y.L. Wang, X. Wang, X. Bai, Y.L. Huang

“…The highest response of DAO in jejunal mucosa was observed for broilers supplemented with 160 mg/kg of Fe. Furthermore, the mRNA expressions of ZO-1 (P < 0.001), occludin (P = 0.004), and claudin-1 (P = 0.007) in jejunal mucosa decreased linearly with increased dietary Fe concentration. …”
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肌球蛋白重链7基因来源的miR-208b-3p促进心肌成纤维细胞中纤维化相关基因表达 by 张梦珍, 翟琳, 郭林林, 朱杰宁, 李晖, 徐金东, 方咸宏, 单志新

“…miR-208b-3p前体与宿主基因Myh7在db/db小鼠心肌中表达显著升高，miR-208b-3p与Myh7 mRNA在乳小鼠来源的mCFs和NMVCs中均有表达，但在NMVCs中水平更高。miR-208b-3p在Ang Ⅱ和G/Go处理后的mCFs和NMVCs中表达均明显升高。…”
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Therapeutic effects of composite probiotics derived from fermented camel milk on metabolic dysregulation and intestinal barrier integrity in type 2 diabetes rats by Tabusi Manaer, Tabusi Manaer, Tabusi Manaer, Tabusi Manaer, Jialehasibieke Sailike, Xin Sun, Baheban Yeerjiang, Baheban Yeerjiang, Baheban Yeerjiang, Baheban Yeerjiang, Xinhua Nabi, Xinhua Nabi, Xinhua Nabi, Xinhua Nabi

“…CPCM lowered serum DAO, D-lactate, and LPS levels (all p &lt; 0.001). mRNA levels of TGR5 and CYP7A1 in the liver, and TGR5 and FXR in the colon, were markedly elevated (all p &lt; 0.001). …”
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GH inhibits ALV-J replication and restricts cell cycle by activating PI3K/Akt signaling pathway by Junliang Xia, Ling Lin, Rongyang Ju, Chengxun Xu, Guodong Mo, Xiquan Zhang

“…In this study, we observed a significant upregulation of GH protein expression in the plasma of ALV infected chickens, and a marked increase in GH mRNA in ALV-J infected cells. We found that lower gp85 expression correlated with higher GH expression in immune tissues, suggesting that GH may inhibit gp85 expression. …”
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The involvement of CgCaspase-3 in regulating the expressions of proinflammatory cytokines through NF-κB/Rel signaling pathway in oysters by Zihan Wang, Xiaopeng Li, Jiejie Sun, Shuyi Mu, Wei Wu, Renle Chang, Lingling Wang, Linsheng Song

“…In haemocytes of Ac-DEVD-CHO-treated and CgCaspase-3-RNAi oysters, the nuclear translocation of NF-κB/Rel was inhibited at 24 h after Vibrio splendidus stimulation. The mRNA expressions of interleukins 17s (CgIL17–2, CgIL17–3, and CgIL17–6), tumor necrosis factor-1 (CgTNF-1), high mobility group box-1 protein (CgHMGB1) and interferon-like protein (CgIFNLP) were significantly decreased in haemocytes of CgCaspase-3-RNAi or CgRel-RNAi oysters, respectively. …”
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Iodine activates NLRP3 inflammasomes in PBMCs of patients with autoimmune thyroiditis and regulates Th1 and Th17 cell differentiation by Ying Wu, Qingling Guo, Yongping Liu, Xun Gong, Wei Sun, Yushu Li, Chenling Fan, Weiping Teng, Zhongyan Shan

“…Methods: We collected PBMCs and serum samples from 50 patients with AIT with normal thyroid function and 50 controls matched for age and sex. In PBMCs, the mRNA and protein expressions of certain inflammasome constituents (e.g., NLRP1, NLRP3, absent in melanoma 2 (AIM2) and caspase-1), interleukin (IL)-1β and IL-18 were assessed using qRT-PCR and western blotting. …”
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Study on the In Vitro and In Vivo Antioxidant Activity and Potential Mechanism of <i>Polygonum viviparum</i> L. by Zhen Yang, Jingyuan Man, Haoyu Liu, Di Wu, Qiangwen Gu, Hongjuan Zhang, Yu Liu, Dan Shao, Baocheng Hao, Shengyi Wang

“…Furthermore, the candidate antioxidant targets of PV were analyzed and screened using a comprehensive network pharmacology method, and their expression were then examined at the mRNA level and protein level. Our results suggest that PV may protect against H₂O₂-induced oxidative damage in macrophages by activating BCL2L1 and inhibiting ESR1, JAK2/STAT3, and MMP2. …”
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Identification of Genetic Markers of <i>APOM</i> and <i>CYP7A1</i> Genes Affecting Milk Production Traits in Chinese Holstein by Yanan Liu, Zijiao Guo, Junqing Ni, Chendong Yang, Bo Han, Yabin Ma, Jianming Li, Guie Jiang, Weijie Zheng, Dongxiao Sun

“…Additionally, with RNAfold Web Server, rs110098953 and rs378530166 changed the mRNA secondary structures of <i>APOM</i> and <i>CYP7A1</i> genes, respectively. …”
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An exploratory study of INPP4B, a biomarker of gemcitabine chemoresistance in pancreatic cancer by REN Jiaqiang, WU Shuai, SU Tong, LI Jie, HAN Liang, WU Zheng

“…RTFQ-PCR and Western blot confirmed that INPP4B mRNA and protein levels were significantly elevated in drug resistant cells compared to wild-type cells (P<0.05). …”
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Unveiling the prognostic value of ARID3A in breast cancer through bioinformatic analysis by Wen-Run Cai, Xu-Gang Sun, Yue Yu, Xin Wang, Xu-Chen Cao, Xiao-Feng Liu

“…This study investigates ARID3A's expression, prognostic relevance, clinicopathological correlations, co-expression profiles, and protein-protein interactions in breast cancer. Methods: ARID3A mRNA and protein expression levels were analyzed using UALCAN, GEPIA databases, and immunohistochemistry from our hospital samples. …”
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Bisphenol S exposure interrupted human embryonic stem cell derived cardiomyocytes differentiation through ER-NF-κB/ERK signaling pathway by Yongru Zhou, Lin Tian, Liang Wang, Wenjing Wu, Baofang Liang, Wei Xiong, Lishi Zhang, Xiaomeng Li, Jinyao Chen

“…The benchmark dose lower confidence limit (BMDL) of the relative expression of cTnT mRNA exposed by BPS during DP3 was the lowest among all the BMDLs of a good fit, with BMDL5 of 1.96 × 10−2 µg/mL, which is lower than the current reported exposure levels of BPS in maternal serum (0.03–0.07 ng/mL) and maternal umbilical cord serum (0.03–0.12 ng/mL).…”
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Clinical and molecular outcomes from the 5-Year natural history study of SSADH Deficiency, a model metabolic neurodevelopmental disorder by Itay Tokatly Latzer, Jean-Baptiste Roullet, Wardiya Afshar-Saber, Henry H. C. Lee, Mariarita Bertoldi, Gabrielle E. McGinty, Melissa L. DiBacco, Erland Arning, Melissa Tsuboyama, Alexander Rotenberg, Thomas Opladen, Kathrin Jeltsch, Àngels García-Cazorla, Natalia Juliá-Palacios, K. Michael Gibson, Mustafa Sahin, Phillip L. Pearl

“…GABA-metabolism in induced GABAergic neurons could be reversed using CRISPR correction of the pathogenic variants or mRNA transfection and SSADHD iPSCs were associated with excessive glutamatergic activity and related synaptic excitation. …”
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Enhancing reproductive capacity in hybrid Red Tilapia (Oreochromis niloticus ×‎ O. mossambicus) via dietary administration of date palm pollen (Phoenix dactylifera L.)‎ by Nashi K. Alqahtani, Hesham S. Ghazzawy, Roshmon Thomas Mathew, Yousef‎ Ahmed Alkhamis, Rashid Saleh Alngada, Moaheda E.H. Eissa, Sameh A. Abdelnour, Sara F. Ghanem, Layla A. Almutairi, El-Sayed Hemdan Eissa

“…The GSI (%) and HIS (%), mean number of fry/fish, egg diameter (mm), and their weights were improved in all DPP fish groups compared to the DPP0 group. The mRNA expression levels of reproductive-related genes, including FSHR, CYP1A, ESR1, FOXL2A, and ESR2A were significantly upregulated in the gonads of fish fed DPP diets compared to the control group (P < 0.05). …”
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The expression of CCL17 and potential prognostic value on tumor immunity in thyroid carcinoma based on bioinformatics analysis by Xue Gu, Bingfeng Chen, Shengcan Zhang, Xinyu Zhai, Ying Hu, Hui Ye

“…GEPIA database was applied to analyze CCL17 mRNA expression in THCA data from TCGA database. …”
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Transient lymphocyte count decrease correlates with oncolytic adenovirus efficacy in humans: mechanistic and biomarker findings from TUNIMO phase I trial by Tuomo Alanko, Akseli Hemminki, Víctor Cervera-Carrascón, Riikka Havunen, Kristian Taipale, Dafne C A Quixabeira, Jorma Sormunen, Katriina Peltola, Suvi Sorsa, Santeri A Pakola, Elise Jirovec, Lyna Haybout, Claudia Kistler, Tatiana V Kudling, Victor Arias, James H A Clubb, Mirte van der Heijden, Teijo Pellinen, Joao M Santos

“…More substantial lymphocyte count change was linked to stronger immune activation in plasma proteome after intravenous TILT-123 and the presence of TILT-123 mRNA in tumors. Regarding other assays. tumor biopsies profiled showed increased amounts of CD8+ T cells, CD4+ T cells and NK cells after intravenous TILT-123, but not after intratumoral TILT-123. …”
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