Expression Analysis of Fibronectin Type III Domain-Containing (FNDC) Genes in Inflammatory Bowel Disease and Colorectal Cancer by Tilo Wuensch, Jonas Wizenty, Janina Quint, Wolfgang Spitz, Madeleen Bosma, Olaf Becker, Andreas Adler, Wilfried Veltzke-Schlieker, Martin Stockmann, Sascha Weiss, Matthias Biebl, Johann Pratschke, Felix Aigner

“…We here aim to comprehensively analyze the mRNA expression of FNDC1, FNDC3A, FNDC3B, FNDC4, FNDC5, and GPR116 in nonaffected and affected mucosal samples of patients with IBD or colorectal cancer (CRC). …”
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Interrelationship between altered metabolites and the gut microbiota in people living with HIV with different immune responses to antiretroviral therapy by Xuebin Tian, Zhongyao Gao, Yiwen Xie, Xiangyun Lu, Yulong Zhao, Peng Yao, Mingqing Dong, Lifeng Yu, Nanping Wu

“…Further experiments showed that Indole-3-acetyl-beta-1-D-glucoside significantly decreased the proportions of CD4+CD57+, effector CD4+, CD4+PD1+, CD8+CD57+, effector CD8+, and CD8+HLA-DR+ T cells. Moreover, mRNA expression analysis showed that Indole-3-acetyl-beta-1-D-glucoside treatment led to a suppression of pro-inflammatory cytokines. …”
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Ginsenoside Rd protects against acute liver injury by regulating the autophagy NLRP3 inflammasome pathway by Xiaomei Zhong, Yibin Sun, Yanxiang Lin, Shan Deng, Huan Wang, Xian Zhou, Jinjian Lu, Yanfang Zheng, Ruoyin Luo, Mingqing Huang, Jianyuan Song

“…It exhibited strong anti-inflammatory effect by reducing inflammation associated protein, such as cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), nod-like receptor protein 3 (NLRP3), associated speck-like protein containing a CARD (ASC), interleukin-18 (IL-18) and interleukin-1β(IL-1β) proteins and the mRNA expression levels of COX-2, Tumor Necrosis Factor α (TNF α), interleukin-6 (IL-6) and iNOS were decreased in liver tissue. …”
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The role of FTO in m6A RNA methylation and immune regulation in Staphylococcus aureus infection-related osteomyelitis by Sijing Liu, Sijing Liu, Sijing Liu, Kai Li, Changhai Long, Mingwu Lao, Biao Ma, Changquan Liu, Haoyuan He, Chunjiang Wang, Wangzhu Chen, Bin Yu

“…The western blotting and qPCR analyses confirmed that the protein expression of FTO was reduced in macrophages after infection with S. aureus, consistent with the observed changes in mRNA expression. Furthermore, we validated that FTO may influence the regulation of inflammation through the FoxO1/NF-kB pathway.ConclusionThe m6A RNA methylation regulator FTO may serve as a potential diagnostic marker and therapeutic target, involved in the pathogenesis of S. aureus infection-related osteomyelitis. …”
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Assessment of prognosis and responsiveness to immunotherapy in colorectal cancer patients based on the level of immune cell infiltration by Kaili Liao, Minqi Zhu, Lei Guo, Zijun Gao, Jinting Cheng, Bing Sun, Yihui Qian, Bingying Lin, Jingyan Zhang, Tingyi Qian, Yixin Jiang, Yanmei Xu, Qionghui Zhong, Xiaozhong Wang

“…ObjectiveTo build a new prognostic risk assessment model based on immune cell co-expression networks for predicting overall survival and evaluating the efficacy of immunotherapy for colon cancer patients.MethodsThe Cancer Genome Atlas (TCGA) database was used to obtain mRNA expression profiling data, clinical information, and somatic mutation data from colorectal cancer patients. …”
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3-methyl-4-nitrophenol disturbs the maternal-to-zygotic transition of early embryos by damaging mitochondrial function and histone modification by Fan Chen, An-Feng Luo, Kai-Xin Pan, Hao Gu, Chang-Fan Zhou, Wei Zeng, Song Liu, Adrian Molenaar, Hong-Yan Ren, Li-Jun Huo, Yan-Zhen Bi

“…SMART-seq2 transcriptome sequencing further confirmed that PNMC treatment disrupted global gene expression in 2-cell embryos, with differentially expressed genes enriched in multiple signaling pathways, including those related to autophagy, apoptosis, fertilization, embryonic development, transcription, and mRNA processing. Integration of transcriptome data with open databases revealed that both zygotic genome activation genes and maternal factors experienced significant transcript-level disruptions. …”
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miR-129-5p Regulates the Immunomodulatory Functions of Adipose-Derived Stem Cells via Targeting Stat1 Signaling by He-Yang Zhang, Yu-Han Wang, Yan Wang, Yan-Nv Qu, Xiao-Hui Huang, Hui-Xin Yang, Chang-Ming Zhao, Youdi He, Si-Wei Li, Jin Zhou, Changyong Wang, Xiao-Xia Jiang

“…When fludarabine, an inhibitor of Stat1 activation, was added to ASCs with miR-129-5p knockdown, iNOS mRNA and protein levels were significantly reduced. …”
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Fosab, but not fosaa, plays important role in learning and memory in fish—insights from zebrafish gene knockout study by Qiuling Wang, Qiuling Wang, Qiuling Wang, Lixin Zhang, Lixin Zhang, Lixin Zhang, Chenyuan Zhu, Chenyuan Zhu, Chenyuan Zhu, Ke Lu, Ke Lu, Ke Lu, Jiaqi Wu, Jiaqi Wu, Jiaqi Wu, Xu-Fang Liang, Xu-Fang Liang, Xu-Fang Liang

“…The average time to complete the trial in fosab−/− zebrafish was significantly higher than in WT zebrafish. The relative mRNA expression level of c-jun in fosab−/− zebrafish was significantly higher than that in WT zebrafish, while the fosaa−/− zebrafish has no discernible trend. …”
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NALCN expression is down-regulated and associated with immune infiltration in gastric cancer by Xuewei Li, Xuewei Li, Na Wu, Chen Wang, Beibei Pei, Xiaoyan Ma, Jun Xie, Jun Xie, Wenhui Yang

“…Consistent with these findings, the expression trend of NALCN mRNA in the GEO database mirrored the experimental results. …”
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Ibuprofen reduces inflammation, necroptosis and protects photoreceptors from light-induced retinal degeneration by Ping-Wu Zhang, Zi-He Wan, Weifeng Li, Abhishek Vats, Kunal Mehta, Laura Fan, Lingli Zhou, Sean Li, Gloria Li, Casey J. Keuthan, Cynthia Berlinicke, Cheng Qian, Noriko Esumi, Elia J Duh, Donald J. Zack

“…Twenty-four hour after LD, retinal mRNA expression for the inflammatory-factors tumor necrosis factor (Tnf), interleukin-1 beta (Il1B), and C-C motif chemokine ligand 2 (Ccl2) increased by 10-, 17-, and 533-fold, respectively; in IBU-treated animals, the expression levels of these inflammatory factors were not significantly different from no-LD controls. …”
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AFP shields hepatocellular carcinoma from macrophage phagocytosis by regulating HuR-mediated CD47 translocation in cellular membrane by Yinglian Pan, Qiushi Yin, Zhaoliang Wang, Gang Wu, Kun Liu, Xiaowei Li, Jinchen Liu, Jiangzheng Zeng, Bo Lin, Wei Li, Mingyue Zhu, Mengsen Li

“…Mechanistically, the results demonstrated that AFP enhanced CD47 membrane translocation by interacting with Hu-Antigen R(HuR), an RNA-binding protein that regulates mRNA stability and translation. AFP alters the subcellular distribution of HuR, increasing its cytoplasmic accumulation and binding to CD47 transcript. …”
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MicroRNA-132, Delivered by Mesenchymal Stem Cell-Derived Exosomes, Promote Angiogenesis in Myocardial Infarction by Teng Ma, Yueqiu Chen, Yihuan Chen, Qingyou Meng, Jiacheng Sun, Lianbo Shao, Yunsheng Yu, Haoyue Huang, Yanqiu Hu, Ziying Yang, Junjie Yang, Zhenya Shen

“…Expression levels of miR-132 in exosomes and HUVECs were quantified by real-time PCR. The mRNA levels of miR-132 target gene RASA1 in HUVECs were quantified by real-time PCR. …”
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The relationship between dietary branched-chain and aromatic amino acids with the regulation of leptin and FTO genes in adipose tissue of patients undergoing abdominal surgery by Farshad Teymoori, Hossein Farhadnejad, Mostafa Norouzzadeh, Mitra Kazemi Jahromi, Niloufar Saber, Ebrahim Mokhtari, Golaleh Asghari, Emad Yuzbashian, Parvin Mirmiran, Alireza Khalaj, Maryam Zarkesh, Mehdi Hedayati, Mohammadreza Vafa

“…Using the quantitative PCR method, leptin and FTO mRNA expression was measured in both visceral and subcutaneous fat tissues. …”
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Small Extracellular Vesicles from Young Healthy Human Plasma Inhibit Cardiac Fibrosis After Myocardial Infarction via miR-664a-3p Targeting SMAD4 by Wang W, Li Y, Zhang C, Zhou H, Li C, Cheng R, Chen X, Pu Y, Chen Y

“…Further studies confirmed SMAD4 as a direct downstream target of miR-664a-3p, whose overexpression could reverse the anti-fibrotic effects of miR-664a-3p.Conclusion: In summary, these findings firstly revealed that Young-sEVs could directly bind to the 3′-untranslated region of SMAD4 mRNA through miR-664a-3p, thereby inhibiting the TGF-β/SMAD4 signaling pathway to protect heart from fibrosis and improve cardiac function. …”
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A novel USP4 inhibitor that suppresses colorectal cancer stemness by promoting β-catenin and Twist1 degradation by Fengtian Li, Yi Zhou, Xinyue Lin, Yaxin Zhang, Qingyong Hu, Enen Zhao, Huali Li, Xingyan Pan, Feng Shu, Kun Zhang, Chengmei Huang, Na Tang, Wenting Liao

“…Methods RNA sequencing was performed to examine the mRNA expression of known deubiquitinating enzymes in CRC tissues from patients with alternate response to chemotherapy. …”
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IL-6/GATA2/SERPINE1 pathway is implicated in regulating cellular senescence after acute kidney injury by Hongshuang Su, Xiaoxi Lin, Ayinuer Paredong, Ayinuer Paredong, Congcong Yao, Yan Zhang, Mengke Geng, Yuqian Guan, Lichao Gong, Feng Jiang, Qi Lv, Songtao Shou, Heng Jin

“…Inhibition of the IL-6 receptor by tocilizumab in these cells led to a reduction in cellular senescence, accompanied by decreased of the cell cycle regulatory proteins P53 and P21 in mRNA and protein levels, while alleviating cell cycle arrest. …”
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Effect of Chloroform Extraction Site of Jiangu Granule on Obteoblast Differentiation <italic>in Vitro</italic> via BMP2/Smad1/Runx2/Osterix Signaling Pathway by ZHOU Fen, SUN Yuqing, SUN Pan, ZHANG Chutian, YANG Juan, LIN Yanping

“…After cell interventions in each experiment group, RT-PCR was used to detect the mRNA expression levels of BMP2, Smad1, Runx2, Osterix and osteoblast differentiation-related genes ALP and Collagen Ⅰ in each group. …”
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Telomere Length Is Associated With Adverse Atrial Remodeling in Patients With Atrial Fibrillation by Kabita Pradhan, Balram Neupane, Paul Niehues, Martin Kirschner, Fabian Beier, Chao‐Chung Kuo, Erika Anneliese Hilbold, Christian Bär, Oana‐Maria Thoma, Maximilian Waldner, Margherita Vieri, Tim H. Brümmendorf, Vithurithra Tharmapalan, Wolfgang Wagner, Michael Kleines, Mahdi Emrani, Matthias Daniel Zink, Andreas Napp, Nikolaus Marx, Michael Gramlich

“…TL was quantified and correlated to low voltage areas. 3′ mRNA sequencing was performed for gene expression profiling. …”
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The mechanism of tea tree oil regulating the damage of hydrogen sulfide to spleen and intestine of chicken by Yachao Wang, Yilei Liang, Yan Huang, Wei wang, Xiaoyan Long, Li Jiang, Tingting Cheng, Jinfeng Du, Xuegang Luo

“…Intestinal findings indicated that TTO treatment enhanced villus height, reduced crypt depth, and upregulated the expression of Claudin 1, Occludin, and ZO-1 mRNA in the jejunum (P < 0.05). After TTO treatment, H2S-induced oxidative stress injury and apoptosis protein expression in spleen were improved (P < 0.05). …”
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MLL4 regulates postnatal palate growth and midpalatal suture development by Jung-Mi Lee, Hunmin Jung, Bruno de Paula Machado Pasqua, Yungki Park, Qinghuang Tang, Shin Jeon, Shin Jeon, Soo-Kyung Lee, Jae W. Lee, Hyuk-Jae Edward Kwon

“…Whole-mount and histological staining at perinatal stages identified that the midline defects in the Mll4-cKO mice emerged as early as 1 day prior to birth, presenting as a widened midpalatal suture, accompanied by increased cell apoptosis in the suture mesenchyme. Genome-wide mRNA expression analysis of the midpalatal suture tissue revealed that MLL4 is essential for the timely expression of major cartilage development genes, such as Col2a1 and Acan, at birth. …”
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