LncRNA PTS-1 Protects Against Osteoarthritis Through the miR-8085/E2F2 Axis by Ma C, Chen Q, Wei YF, Chen SW, Liu H, Xin F, Ren YX

“…Cell viability, proliferation, and apoptosis rates, along with the mRNA and protein levels of apoptosis-related markers (cleaved Caspase 3, cleaved Caspase 9, Bcl-2, Bax), ECM metabolism markers (MMP-3, MMP-13, aggrecan, collagen II), and inflammation-related markers (IL-1β, IL-6, TNF-α) were evaluated using Cell Counting Kit-8, Toluidine Blue staining, Alcian Blue staining, flow cytometry, qRT-PCR, immunofluorescence, and Western Blot. …”
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New evidence on the nephrotoxicity of fine particulate matter: Potential toxic components from different emission sources by Fu-Jen Cheng, Chien-Er Huang, Pei-Shih Chen, Yu-Lun Tseng, Chung-Shin Yuan, Ching-Shu Lai

“…Exposure to PM2.5 water extract increased the mRNA levels of tumor necrosis factor-α, interleukin-6, and nitrite production in RAW264.7 macrophages. …”
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High-fat diet promotes gestational diabetes mellitus through modulating gut microbiota and bile acid metabolism by Lei Yao, Xuefei Zhou, Xianqi Jiang, Hao Chen, Yuanliang Li, Xiao Xiong, Yan Tang, Haogang Zhang, Pengfei Qiao

“…Expression levels of Fxr, Shp and Fgf15 mRNA and protein were detected by quantitative reverse transcription polymerase chain reaction and western blot, respectively.ResultsOur data indicated that high fat diet (HFD) was linked with higher prevalence of GDM, and HFD was positively associated with poor prognosis in GDM patients. …”
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Global vaccine research and application hotspots and trends: a systematic bibliometric analysis based on SCIE highly cited papers by Liming Liang, Xinyu Wang, Yue Zhu, Xu Xia, Runfeng Shi, Huilin Cao, Meixin He, Yuyu Wang, Caizhen You

“…The most highly cited paper was ‘Safety and Efficacy of the BNT162b2 mRNA COVID-19 Vaccine’ from the New England Journal of Medicine, which was cited 9435 times in total. …”
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FKBP5 as a key regulator of metabolic processes in birds: Insights from chicken pectoral muscle by Pengfei Du, Xiangli Zhang, Yao Zhu, Ziyang Wang, Xuemeng Si, Huaiyong Zhang, Yanqun Huang, Wen Chen

“…Insulin regulates FKBP5 expression in pectoral muscles in a time-dependent manner, while pyruvate increases FKBP5 levels and fasting/energy restriction reduces FKBP5 mRNA expression in the pectoral muscles.…”
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Effect of fructo-oligosaccharides on growth performance and meat quality in broilers by ZhiHui Yan, XiaoWu Tang, RunTao Wu, Can Yang, YunMiao Jiang, Xuan Wang, QingHai Tang, YongLing Hu, LeLi Wang, Zhi Jiang

“…There was an increasing trend for total antioxidant capacity (T-AOC) activity of thigh muscle in FOS group compared to CON group (P = 0.053). Relative mRNA expression of breast catalase (CAT), superoxide dismutase 1 (SOD1), thioredoxin reductase 1 (TXNRD) were up-regulated by FOS supplementation compared with CON group (P < 0.05). …”
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Intrahepatic CD161hiCD8+T Cell Recruitment Has a Pathogenetic Potential in Chronic HBV Infection by Jianfei Li, Qian Liu, Wanlu Duan, Zhi Duan, Futing Liu, Mengqi Ruan, Qiyin Zong, Hao Zhang, Qiang Zhou, Qin Wang

“…Accordingly, the CXCL16 mRNA expression in the liver tissue of CHB‐replication mice was markedly higher than in acute HBV‐replicating and control mice. …”
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二甲双胍和孟鲁司特在急性和慢性过敏性哮喘小鼠模型中的作用 by 郭妍蓉, 严彦

“…各实验组小鼠处死后苏木素-伊红染色和马松染色分析小鼠的肺部病理特征；采用Image J软件进行气道炎症细胞浸润评分和胶原沉积评分，以及分析支气管形态参数，包括支气管壁厚度和支气管平滑肌厚度；采用ELISA方法检测小鼠血清总IgE水平，qRT-PCR检测炎症因子<italic>IL-4</italic>、<italic>IL-5</italic>、<italic>IL-13</italic> mRNA转录水平。结果慢性模型小鼠的血清总IgE水平、气道周围胶原沉积程度、气道平滑肌厚度显著高于急性模型小鼠（<italic>P</italic>＜0.05）。…”
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Induction of phospholipase A2 group 4C by HCV infection regulates lipid droplet formation by Masahiko Ito, Jie Liu, Masayoshi Fukasawa, Koji Tsutsumi, Yumi Kanegae, Mitsutoshi Setou, Michinori Kohara, Tetsuro Suzuki

“…Among the transcripts associated with phosphatidylcholine biosynthesis, upregulation of PLA2G4C mRNA was most pronounced following HCV infection. …”
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Complete mitochondrial genomes of Culicoides brevitarsis and Culicoides imicola biting midge vectors of Bluetongue Virus by Khandaker Asif Ahmed, Anjana Karawita, Melissa J. Klein, Luana Fiorella Mincarelli, Barbara Secondini, Giuseppe Satta, Massimo Ancora, Cipriano Foxi, Marco Di Domenico, Michela Quaglia, Maria Goffredo, Alessio Lorusso, Cesare Cammà, Leon Court, Rahul V. Rane, Tom K. Walsh, Prasad N. Paradkar, Debbie Eagles, Gunjan Pandey, Christopher M. Hardy

“…Eleven protein-coding genes encode a full TAA stop codon, while two (ND5, COX3) are completed by mRNA polyadenylation. Phylogenetic analysis of the mitogenomes showed C. brevitarsis and C. imicola form a monophyletic group. …”
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CFTR Expression Analysis for Subtyping of Human Pancreatic Cancer Organoids by Alexander Hennig, Laura Wolf, Beatrix Jahnke, Heike Polster, Therese Seidlitz, Kristin Werner, Daniela E. Aust, Jochen Hampe, Marius Distler, Jürgen Weitz, Daniel E. Stange, Thilo Welsch

“…Human PDAC organoids can be classified into known subtypes based on KRT81 and CFTR immunoreactivity. CFTR and HNF1A mRNA levels correlated well. Furthermore, subtype-specific immunoreactivity matched well between PDAC organoids and the respective primary tumor tissue. …”
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Apigenin inhibits NLRP3 inflammasome activation in monocytes and macrophages independently of CD38 by Knut Husø Lauritzen, Knut Husø Lauritzen, Kuan Yang, Michael Frisk, Mieke C. Louwe, Maria Belland Olsen, Maria Belland Olsen, Mathias Ziegler, William E. Louch, Bente Halvorsen, Bente Halvorsen, Pål Aukrust, Pål Aukrust, Arne Yndestad, Øystein Sandanger, Øystein Sandanger

“…IL-1β and TNF secretion and mRNA expression, as well as N-terminal gasdermin-D formation were quantified. …”
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Wu-Mei-Wan enhances brown adipose tissue function and white adipose browning in obese mice via upregulation of HSF1 by Shen Chen, Kexin Nie, Hongzhan Wang, Yang Gao, Xinyue Jiang, Hao Su, Zhi Wang, Yueheng Tang, Fuer Lu, Hui Dong, Jingbin Li

“…Protein expression was assessed using western blot, immunohistochemistry, immunofluorescence and RT-qPCR were employed to detect the mRNA levels. Statistical analyses included t-tests, ANOVA, and non-parametric tests like the Mann–Whitney U test or Kruskal–Wallis test. …”
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Mechanism of Inhibition of Inflammation in Osteoarthritis by Tougu Xiaotong Capsule via Regulating Ras-Raf-MEK1/2-ERK1/2 Signaling Pathway by Munan LIN, Xiang SHAO, Limei XU, Xin ZHANG, Zaishi ZHU, Yidan HAN, Xinwei DUAN, Jianmei CHEN, song GAO, Xihai LI

“…Isolated chondrocytes of passage two were divided into blank group, model group(treatment with 10 ng/mL of LPS for 8 h), inhibitor group(treatment with 10 ng/mL of U0126 for 30 min followed by 10 ng/mL of LPS for additional 8 h)and TXC group(treatment with 10 ng/mL of LPS plus 300 μg/mL of TXC for 8 h).Western blot was carried out to detect the expression of Ras, Raf, p-ERK1/2, MEK1/2, MMP-3 and MMP-13.qPCR was used to detect the mRNAs expression of MEK1/2, ERK1/2, MMP-3 and MMP-13, as well as the expression of miR-27b, miR-34a and miR-146a.Results:1)The cytoplasm of isolated cells(P2)from the cartilage of knee joint of 4-week-old SD male rats was stained brown by typeⅡcollagen immunohistochemical staining as compared with the negative control cells, which indicated that the extracted cells were chondrocytes.2)The expression of Ras, Raf, MEK1/2, MMP-3, MMP-13 in the model group was significantly higher than that in the blank group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05), but the expression of p-ERK1/2 had no significant difference(<italic>P</italic>&gt;0.05);the expression of Ras, Raf, p-ERK1/2, MEK1/2, MMP-3, MMP-13 in both the inhibitor group and the TXC group was lower than that in the model group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05), but the expression of MEK1/2 in the TXC group had no significant difference compared to that in the model group(<italic>P</italic>&gt;0.05).3)The mRNA expression of MEK1/2, ERK1/2, MMP-3 and MMP-13 in the model group was significantly higher than that in the blank group, respectively(<italic>P</italic>&lt;0.01);their expression in both the inhibitor group and the TXC group was lower than that in the model group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05).4)The expression of miR-27b in the model group was significantly lower than that in the blank group(<italic>P</italic>&lt;0.01), but its expre-ssion in both the inhibitor group and the TXC group was higher than that in the model group(<italic>P</italic>&lt;0.01);the expression of both miR-34a and miR-146a in the model group was significantly higher than that in the blank group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05);however, their expression in both the inhibitor group and the TXC group was lower than that in the model group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05).Conclusion:Tougu Xiaotong capsule regulates the expression of key factors in Ras-Raf-MEK1/2-ERK1/2 signaling pathway and miR-27b, miR-34a and miR-146a to regulate the inflammatory of chondrocytes, thereby inhibiting osteoarthritis inflammation and protecting articular cartilage.…”
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NCAPD3-mediated ferroptosis of 2,3,5,4’-Tetrahydroxystilbene-2-O-β-D-glucoside inhibits proliferation in T47D cells by Jianfen Shen, Shuo Zhang, Shuo Zhang, Yan Song, Yan Song, Leiming Yang, Leiming Yang, Qi Huang, Qi Huang, Pengyu Wang, Pengyu Wang, Youzhi Zhang, Youzhi Zhang

“…And siRNA of Ncapd3 was transfected into T47D cells to detect the expression levels of ferroptosis-related proteins (WB) and cell viability (MTT).Results40 overlapping transcripts of DETs and diAS were obtained consistent with the analysis of full-length transcriptome sequencing, and Ncapd3 (Ncapd3-203) is key gene (transcript), which was also highly expressed in breast cancer and THSG could inhibit the mRNA and protein expression. Moreover, THSG could induce cell cycle arrest in G2/M stage and reduce ferroptosis-related protein expression (xCT and GPx4). …”
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Preconditioning of Rat Bone Marrow-Derived Mesenchymal Stromal Cells with Toll-Like Receptor Agonists by Fabiana Evaristo-Mendonça, Gabriela Sardella-Silva, Tais Hanae Kasai-Brunswick, Raquel Maria Pereira Campos, Pablo Domizi, Marcelo Felippe Santiago, Ricardo Augusto de Melo Reis, Rosalia Mendez-Otero, Victor Túlio Ribeiro-Resende, Pedro Moreno Pimentel-Coelho

“…The data showed that rBM-MSCs expressed TLR3, TLR4, and MDA5 mRNA and were able to internalize polyinosinic-polycytidylic acid (Poly(I:C)), a TLR3/MDA5 agonist. rBM-MSCs were then stimulated with Poly(I:C) or with lipopolysaccharide (LPS, a TLR4 agonist) for 1 h and were grown under normal culture conditions. …”
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Significance of Biogenetic Markers in Giant Cell Tumor Differentiation and Prognosis: A Narrative Review by Muhammad Taqi, Haseeb ul Rasool, Mobeen Zaka Haider, Munjed Al Muderis

“…Large amounts of osteoclast-related mRNA (cathepsin K, tartrate-resistant acid phosphatase, and matrix metalloproteinase9) in GCTs (<i>p</i> < 0.05) are associated with the grade of bone resorption. …”
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The hematopoietic function, histological characteristics, and transcriptome profiling of Wanxi white geese ovary during nesting and late-laying stages by Chunfang Zhao, Tao Jin, Kefeng Yang, Xinyu Liu, Man Ren, Deyong She, Qianqian Hu, Shenghe Li

“…In the nesting period, the ovaries were degenerated and had many primary follicles without follicular development. Analysis of mRNA-lncRNA expression revealed 1,257 DEGs between the nesting and the late-laying stages, of which 841 were up-regulated and 416 were down-regulated DEGs. …”
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Deregulated methylation and expression of PCDHGB7 in patients with non-small cell lung cancer: a novel prognostic and immunological biomarker by Yue Yuan, Yue Yuan, Xin Nie, Jiayi Gao, Jiayi Gao, Yumeng Tian, Yumeng Tian, Liuer He, Liuer He, Xue Wang, Ping Zhang, Junling Ma, Lin Li, Lin Li

“…The methylation level of PCDHGB7 was upregulated in tumor tissue and negatively correlated with PCDHGB7 mRNA level. In immunotherapy cohort studies, patients with higher PCDHGB7 tissue expression showed worse prognosis. …”
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Nitidine chloride inhibits the progression of hepatocellular carcinoma by suppressing IGF2BP3 and modulates metabolic pathways in an m6A-dependent manner by Dan-dan Xiong, Zhen-dong Chen, Jian-di Li, Yu-long Deng, Rong-quan He, Zhi-guang Huang, San-qi An, Yi-wu Dang, Gang Chen

“…Further MeRIP-seq and RIP-seq revealed 197 genes interacting with IGF2BP3, downregulated at mRNA and m6A levels after NC treatment, primarily associated with multiple metabolism-related pathways. …”
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