A Study on the Dietary Yeast Polysaccharide Supplementation in Growth Performance, Antioxidant Capacity, and Immunity of Juvenile Largemouth Bass (<i>Micropterus salmoides</i>) by Junjie Qin, Haifeng Mi, Mingchun Ren, Dongyu Huang, Hualiang Liang, Lu Zhang, Tao Teng, Heng Yin

“…Moreover, the 0.05Y group showed significantly higher immune-related gene (nuclear factor-kappa B, interleukin-8, and interleukin-10) mRNA expression. Altogether, our results indicate that dietary YPS supplementation enhances the antioxidant and immune capacity of <i>M. salmoides</i>, but with no positive effect on their growth.…”
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Effects of Probiotics Red Yeast Rhodotorula paludigena CM33 on enhancing color pigmentation, antioxidant activity, immune response, intestinal microbiota, and growth in a commercia... by Jirawadee Kaewda, Papungkorn Sangsawad, Surintorn Boonanuntanasarn, Pimpisut Manassila, Apichat Boontawan, Mariena Ketudat‑Cairns, Cheeranan Sriphuttha, Chatsirin Nakharuthai

“…These findings correspond with the mRNA expression results of growth-related genes (insulin-like growth factor-1 (IGF-I) and IGF-II), antioxidant genes (superoxide dismutase (SOD) and catalase (CAT)), and pro-inflammatory genes (interleukin-1 beta (IL-1β) and IL-6). …”
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Spinal CRH facilitates the micturition reflex via the CRH2 receptor in rats with normal bladder and bladder outlet obstruction by Risa Shinoki, Ryosuke Jikuya, Takuma Nirei, Takeshi Fukazawa, Hiroki Takizawa, Mari Hioki, Sachi Kawaura, Tomoyuki Tatenuma, Go Noguchi, Daiki Ueno, Yusuke Ito, Mitsuru Komeya, Kentaro Muraoka, Hisashi Hasumi, Kazuki Kobayashi, Masahito Takiguchi, Kengo Funakoshi, Kazuhide Makiyama, Naoki Aizawa, Hiroki Ito

“…Real-time PCR showed higher CRHR2 mRNA expression in the spinal cord and DRG than in the bladder in both groups. …”
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A novel approach to regulate glucose uptake in an anaplastic thyroid cancer cell line by Shabnam Heydarzadeh, Ali Asghar Moshtaghie, Maryam Daneshpour, Mehdi Hedayati

“…Determination of GLUT1 and GLUT3 mRNA and protein expression in SW1736 cells was performed by qRT-PCR and western blotting. …”
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Protective Effects of Thalidomide on High-Glucose-Induced Podocyte Injury through In Vitro Modulation of Macrophage M1/M2 Differentiation by Hui Liao, Yuanping Li, Xilan Zhang, Xiaoyun Zhao, Dan Zheng, Dayue Shen, Rongshan Li

“…The present study explored the effects of Tha on mRNA and protein expressions of inducible nitric oxide synthase (iNOS), tumor necrosis factor- (TNF-) α, mannose receptor (CD206), and arginase- (Arg-) 1 in HG-activated macrophages. iNOS and TNF-α are established as markers of classically activated macrophage (M1). …”
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Interaction Between Malat1 and miR-499-5p Regulates Meis1 Expression and Function with a Net Impact on Cell Proliferation by Salma A. Fahim, Manon Ragheb, Ibrahim Hassan Fayed, Aya Osama, Ahmed Karam, Sameh Magdeldin, Rana Metwale, Mohamed Dief Allah Abdalmoneam Elsayed, Ahmed Abdellatif, Hesham A. Sadek, Shereen Ahmed El Sobky, Nada El-Ekiaby, Injie Omar Fawzy, Ahmed Ihab Abdelaziz

“…For the first time, we demonstrated that the overexpression of miR-499-5p led to the downregulation of Meis1 mRNA and protein in C166 cells by directly binding to its 3’UTR. …”
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Effects of milk extracellular vesicles from Holstein Friesian and Brown Swiss heat-stressed dairy cows on bovine mammary epithelial cells by S. Castellani, L. Basiricò, A. Maggiolino, C. Lecchi, P. De Palo, U. Bernabucci

“…Cell viability and apoptosis assay, reactive oxygen species production, and mRNA expression of heat shock proteins and antioxidant genes by reverse transcription and real time PCR were determined. …”
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Selectively expressed RNA molecules as a versatile tool for functionalized cell targeting by Frederik Rastfeld, Marco Hoffmann, Sylvie Krüger, Patrick Bohn, Anne-Sophie Gribling-Burrer, Laura Wagner, Nils Hersch, Carina Stegmayr, Lukas Lövenich, Sven Gerlach, Daniel Köninger, Christina Hoffmann, Helene L. Walter, Dirk Wiedermann, Hajaani Manoharan, Gereon R. Fink, Rudolf Merkel, Heribert Bohlen, Redmond P. Smyth, Maria A. Rueger, Bernd Hoffmann

“…The specific combination of the molecular properties of antisense technology and mRNA therapy with functional RNA secondary structures allowed us to develop selectively expressed RNA molecules for medical applications. …”
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吴茱萸次碱通过Sirt1改善血管平滑肌细胞钙化 by 邵静, 陈安, 张秀丽, 杨平珍

“…另外，高磷高钙可下调血管平滑肌细胞Sirt1的表达水平，qRT-PCR和Western blot结果证实吴茱萸次碱可明显地上调Sirt1 mRNA（0.35±0.06、0.75±0.11；0.22±0.08、0.87±0.13；P<0.05）和Sirt1的蛋白（0.38±0.09、0.71±0.13；P<0.05）的表达水平。…”
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Differential Effects of Angelicin Analogues on NF-κB Activity and IL-8 Gene Expression in Cystic Fibrosis IB3-1 Cells by Ilaria Lampronti, Maria Giulia Manzione, Gianni Sacchetti, Davide Ferrari, Susanna Spisani, Valentino Bezzerri, Alessia Finotti, Monica Borgatti, Maria Cristina Dechecchi, Giorgia Miolo, Giovanni Marzaro, Giulio Cabrini, Roberto Gambari, Adriana Chilin

“…Other analogues displayed higher activities; in particular, the most interesting compounds showing relevant anti-inflammatory effects were found to cause 56–83% reduction of IL-8 mRNA expression at low concentrations (1–10 μM), without changes in cell proliferation pattern, demonstrating their potential interest for a possible development of anti-inflammatory therapy of cystic fibrosis.…”
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Implication of fibroblast growth factor 7 in ovarian cancer metastases and patient survival by Laura F. Mortan, Laura F. Mortan, Brooke A. Meelheim, Justin Garland, Justin Garland, Jacqueline A. Bohn, Zitha Redempta Isingizwe, Doris M. Benbrook, Doris M. Benbrook

“…Significances of differential gene expression and patient prognosis were determined by volcano plot and log-rank test, respectively.ResultsTumor transcriptomes exhibited higher endothelial cells, oxygenation, proteasome activity, and metabolism in comparison to ascites, but similar percentages of cancer-associated fibroblasts and immune cells. FGF7 mRNA was significantly higher in mouse tumors compared to ascites. …”
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FadA antigen of Fusobacterium nucleatum: implications for ceRNA network in colorectal cancer and adenomatous polyps progression by Sama Rezasoltani, Elahe Shams, Moein Piroozkhah, Yaser Aidi, Mehdi Azizmohammad Looha, Parmida Bagheri, Roudabeh Behzadi Andouhjerdi, Amir Sadeghi, Leili Rejali, Ehsan Nazemalhosseini-Mojarad

“…Then, the ANXA2 ceRNA network was constructed. Subsequently, the mRNA, miRNA, and lncRNA expression levels were examined in intestinal biopsy specimens from 30 healthy controls, 30 Adenoma patients, and 30 cases of CRC stage I using qRT-PCR. …”
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tiRNA-Gln-CTG is Involved in the Regulation of Trophoblast Cell Function in Pre-eclampsia and Serves as a Potent Biomarker by Yixiao Wang, Xiaohong Ji, Hengmei Shi, Sicong Liu, Hong Yu

“…In vitro experiments revealed that tiRNA-Gln-CTG influences the proliferation, cycling, migration, and invasion of HTR-8/SVneo cells, possibly by targeting the 3′UTR region of thrombospondin-2 messenger ribonucleic acid (mRNA) for degradation. Extracellular vesicle (EV) carriers may mediate the level of tiRNA-Gln-CTG in the circulation. …”
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Mechanistic investigation and the optimal dose based on baicalin in the treatment of ulcerative colitis–A preclinical systematic review and meta-analysis by Jinchen Chong, Zepeng Chen, Jiaze Ma, Linhai He, Yijia Zhu, Zhihua Lu, Zhengxi Qiu, Chen Chen, Yugen Chen, Feng Jiang

“…Baicalin significantly modulated various biomarkers: HCS (SMD = -3.91), DAI (MD = -2.75), spleen index (MD = -12.76), MDA (SMD = -3.88), IL-6 (SMD = -10.59), IL-1β (SMD = -3.98), TNF-α (SMD = -8.05), NF-κB (SMD = -5.46), TLR4 (MD = -0.38), RORγ (MD = -0.89), MCP-1 (MD = -153.25), MPO (SMD = -7.34), Caspase-9 (MD = -0.93), Caspase-3 (MD = -0.45), FasL (MD = -1.20)) and enhanced BWC (MD = 0.06), CL (MD = 1.39), ZO-1 (MD = 0.44), SOD (SMD = 3.04), IL-10 mRNA (MD = 3.14), and FOXP3 (MD = 0.45) levels. Baicalin’s actions may involve the PI3K/AKT, TLR4/NF-κB, IKK/IKB, Bcl-2/Bax, Th17/Treg, and TLRs/MyD88 pathways. …”
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Construction and function validation of inducible immortalized gene integration vectors by YUE Wei, YANG Yue, QIAN Baohua, LI Yanxin, GU Haihui

“…Lentiviral packaging, cell transfection, mRNA expression analysis, Western blotting for protein detection, green fluorescent protein (GFP) visualization, and cell proliferation assays were conducted to evaluate transfection efficiency and assess the regulatory effects of Tet on gene expression in 293T and MEF cells. …”
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Multi-omics reveals that ST6GAL1 promotes colorectal cancer progression through LGALS3BP sialylation by Yuanchao Shi, Zhenzhong Pan, Jingwei Duan, Zexing Wang, Yiliang Fang, Bo Tang, Quanlin Guan

“…We found that ST6GAL1 was significantly upregulated in tumor samples than in matched normal samples by analyzing fresh clinical samples from public databases (mean mRNA expression level: tumor vs. normal samples = 0.002712:0.000966, P < 0.05, n = 22). …”
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GPR137-RAB8A activation promotes ovarian cancer development via the Hedgehog pathway by Chao Tang, Lin Li, Chongying Zhu, Qiang Xu, Zihao An, Shouying Xu, Chao Lin

“…Mechanistically, RAB8A was identified as a downstream target of GPR137, and GPR137 promotes RAB8A expression by promoting RAB8A mRNA stability. By RNA-sequencing and experiments in vitro using multiple ovarian cancer cell models as well as in vivo using subcutaneous xenografts assay and in situ transplantation ovarian cancer model in nude mice, we further demonstrated that RAB8A positively mediated OC progression through activating HH signaling pathway by disassociating the protein–protein complex formation of GLI and SuFu (Suppressor of Fused), which reciprocally enhanced GPR137 activity, forming a regulation loop between HH signaling and GPR137. …”
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High spatial resolution gene expression profiling and characterization of neuroblasts migrating in the peri-injured cortex using photo-isolation chemistry by Takuya Miyamoto, Kazuya Kuboyama, Mizuki Honda, Mizuki Honda, Yasuyuki Ohkawa, Shinya Oki, Shinya Oki, Kazunobu Sawamoto, Kazunobu Sawamoto

“…Indeed, neuroblasts migrating in the peri-injured cortex had significantly lower Cyclin D2 mRNA and Ki67 protein expression levels than those in the V-SVZ. …”
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Plasma prolactin axis shift from placental to pituitary origin in late prepartum mice by Taku James Sairenji, Shinnosuke Masuda, Yuya Higuchi, Mitsue Miyazaki, Hiroyuki Yajima, Oh Kwan Ee, Yuki Fujiwara, Takuya Araki, Noriaki Shimokawa, Noriyuki Koibuchi

“…We also found that maternal plasma progesterone regression precedes the onset of the PRL shift. The level of Prl3b1 mRNA was determined by RT-qPCR in the placenta and remained stable until parturition, implying that PRL3B1 peptide production or secretion was suppressed. …”
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Impaired hippocampal neurogenesis associated with regulatory ceRNA network in a mouse model of postoperative cognitive dysfunction by Jingrun Lin, Xiaoqiu Zhu, Xuan Li, Yu Hong, Yaohui Liang, Siqi Chen, Chenzhuo Feng, Lin Cao

“…Cognitive function was assessed 7–10 days post-surgery using open field, Y-maze, and novel object recognition tests. Hippocampal mRNA expression was analyzed using Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment. …”
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