环缩酚肤抑制视网膜表达V E G F 及P E C A M 基因和血管增生 by 梁小玲, 黄水盛, 陈浩宇, 郭晓会, 于珊珊, 谢素贞, 颜坚, 唐仕波

“…5d后取出左侧眼，分离视网膜并抽提RNA，用荧光定量PCR测出上述基因含量，并分别求出每个靶基因与标准基因S16含量的比率；右眼经心脏荧光素灌注后取眼球做视网膜平铺片，用图像分析软件定量计算视网膜新生血管的面积。【结果】视网膜中VEGF／S16的mRNA比率为：第1组(0.554±0.050)，第2组(0.355±0.037)，第3组(0.287±0.051)；PECAM／S16为：第1组(2.050±0.249)，第2组(1.228±0.153)，第3组(1.027±0.210)。…”
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ATG5 and ATG7 Expression Levels Are Reduced in Cutaneous Melanoma and Regulated by NRF1 by Živa Frangež, Deborah Gérard, Zhaoyue He, Marios Gavriil, Yuniel Fernández-Marrero, Yuniel Fernández-Marrero, S. Morteza Seyed Jafari, Robert E. Hunger, Philippe Lucarelli, Shida Yousefi, Thomas Sauter, Lasse Sinkkonen, Hans-Uwe Simon, Hans-Uwe Simon, Hans-Uwe Simon, Hans-Uwe Simon

“…We then show that nuclear respiratory factor 1 (NRF1) stimulates the production of mRNA and protein as well as the promoter activity of ATG5 and ATG7. …”
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Effects of dietary Bacillus subtilis 14823 on growth performance, gut barrier integrity and inflammatory response of broilers raised in a stressful tropical environment by Sunisa Khongthong, Pipat Piewngam, Natthrit Roekngam, Prapot Maliwan, Sonsawan Kongpuckdee, Juthatip Jeenkeawpleam, Prawit Rodjan

“…Furthermore, at d 14 and 35, mRNA expressions of occludin, claudin-1, and tight junction protein-1 in the jejunum were upregulated (P < 0.05), and expression levels of five inflammatory cytokine genes were downregulated in the ileum (P < 0.05). …”
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Increased Frequency of Circulating Follicular Helper T Cells in Patients with Rheumatoid Arthritis by Jie Ma, Chenlu Zhu, Bin Ma, Jie Tian, Samuel Essien Baidoo, Chaoming Mao, Wei Wu, Jianguo Chen, Jia Tong, Min Yang, Zhijun Jiao, Huaxi Xu, Liwei Lu, Shengjun Wang

“…Furthermore, expression of Bcl-6 mRNA and plasma IL-21 concentrations in RA patients was increased. …”
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Dietary supplementation with 15% tomato pomace (Solanum lycopersicum L.) improves sperm production and antioxidant status in aged male broiler breeders by Amir Mosayyeb Zadeh, Seyyed Ali Mirghelenj, Mohsen Daneshyar, Mohsen Eslami, Mohammad Amir Karimi Torshizi, Mahdi Zhandi, Touba Nadri, John Patrick Kastelic, Peyman Hasanloo, Mehdi Nabiloo

“…The T-15 and L-10 groups had higher mRNA expressions of AKT1 and Nrf2 (P<0.05). In conclusion, in aged male broiler breeders, up to 15% TP supplementation is recommended to improve reproductive performance by enhancing testes histology and antioxidant status.…”
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CLEC11A-Driven Molecular Mechanisms in Intervertebral Disc Degeneration: A Comprehensive Multi-Omics Study by Jiang N, Wang Q, Hu Z, Tian X

“…In vitro experiments demonstrated that overexpression of CLEC11A in nucleus pulposus cells significantly enhanced mRNA and protein expression of IVDD-related inflammatory markers; conversely, silencing CLEC11A markedly reduced these expressions. …”
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3-Hydroxybutyrate alleviates septic liver injury by promoting macrophage polarization from M1 to M2 by Hu Jingjuan, Deng Fan, Sun Qishun, Min Yue, Feng Siyuan, Wu Ling, Chen Xiaofeng, Liu Kexuan

“…The survival rate, liver histopathological damage, serum levels of Alanine Transaminase(ALT) and Aspartate Aminotransferase(AST), and hepatic mRNA expression of inflammatory cytokines IL-1β and IL-6 were evaluated. …”
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Expression of lipid metabolism- related genes, body composition and inflammation: Effects of sodium butyrate supplementation in subjects with obesity by Parichehr Amiri, Seyed Ahmad Hosseini, Maryam Saghafi-Asl, Amin Akbari-Naserkiadeh, Seyedeh Asal Jenani Zavieh, Sara Arefhosseini, Samira Asghari, Neda Roshanravan

“…At the end of the trial, the mRNA expression levels of adiponectin receptor-1, adiponectin receptor-2 and uncoupling protein-3, significantly increased in NaBu group to almost two-fold higher levels, compared with placebo group (p = 0.030, p = 0.030 and p = 0.010, respectively). …”
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Lipopolysaccharide (LPS) Aggravates High Glucose- and Hypoxia/Reoxygenation-Induced Injury through Activating ROS-Dependent NLRP3 Inflammasome-Mediated Pyroptosis in H9C2 Cardiomyo... by Zhen Qiu, Yuhong He, Hao Ming, Shaoqing Lei, Yan Leng, Zhong-yuan Xia

“…The concentrations of IL-1β and IL-18 in the supernatants were assessed by ELISA. The mRNA levels of NLRP3, ASC, and caspase-1 were detected by qRT-PCR. …”
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Cuproptosis-Related Signature Predicts the Prognosis, Tumor Microenvironment, and Drug Sensitivity of Hepatocellular Carcinoma by Xiangjun Qi, Jiayun Guo, Guoming Chen, Caishan Fang, Leihao Hu, Jing Li, Chi Zhang

“…However, the role of CRGs in Hepatocellular Carcinoma (HCC) remains unknown. Method. The mRNA transcriptome profiling data, somatic mutation data, and copy number gene level data of The Cancer Genome Atlas-Liver Hepatocellular Carcinoma project (TCGA-LIHC) were downloaded for subsequent analysis. …”
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Evaluation of longan (Dimocarpus longan) peel powder as fruit by-product additive in Nile tilapia (Oreochromis niloticus) feed: Effects on growth, immunity, and immune-antioxidant... by Supreya Wannavijit, Piyatida Outama, Chinh Le Xuan, Camilla Maria Fontana, Marina Paolucci, Md Afsar Ahmed Sumon, Ehab El-Haroun, Hien Van Doan

“…Similarly, analysis of mRNA transcripts of immune (IL-1, IL-8, and LBP) and antioxidant (GSTa, GPX, and GSR) gene expressions showed a significant upregulation (P < 0.05) in LP-fed diet groups compared to the control group. …”
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Jiawei Suanzaoren decoction for the treatment of perimenopausal insomnia: clinical observation and experimental study by Li-Qiong Chen, Yong-Can Zhou, Fang Ning, Ming-Jin Zhu

“…The mechanism behind this improvement may be related to the regulation of Estradiol (E2), Follicle-Stimulating Hormone (FSH), and luteinizing hormone (LH) levels, as well as the mRNA expression levels of 5-HT1a, 5-HT2a, GABAARα1, and GABAARγ2 receptors in the hypothalamus.…”
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Klotho enhances stability of chronic kidney disease atherosclerotic plaques by inhibiting GRK2/PLC-β-mediated endoplasmic reticulum stress in macrophages via modulation of the ROS/... by Zhe Li, Jing Li, Lin Li, Qian Wang, Qian Zhang, Ling Tian, Chenchen Li

“…This study investigates the mechanism by which Klotho enhances the stability of atherosclerotic plaques in chronic kidney disease. apoE-/- knockout mice and C57BL/6 mice underwent 5/6 nephrectomy and then klotho-NC and klotho-mimic groups were set up to be fed a high-fat chow diet and a dummy group was created to be fed a normal chow diet. qPCR detected relative mRNA expression of klotho. Oil Red O and HE staining assessed lipid proportion in the aorta. …”
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Association of serum and local GRP78 and CHOP expressions with disease progression in patients with non-traumatic osteonecrosis of femoral head by Peng Zhang, Qing-He Ye, Wen-Xiu Zhu, Yong-Heng Zhao, Hong-Xun Zhu, Biao-Fang Wei

“…A total of 42 ONFH patients at FICAT stage 3, 40 patients at FICAT stage 4, and 49 FNF patients received total hip replacement (THR). The mRNA and protein levels of CHOP and GRP78 were elevated in necrotic area compared to the non-necrotic area of ONFH patients and the FH tissues of FNF patients with statistical significance. …”
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Puerariae Radix protects against ulcerative colitis in mice by inhibiting NLRP3 inflammasome activation by Yu Ga, Yuanyuan Wei, Qingyu Zhao, Yimeng Fan, Yannan Zhang, Zhifang Zhang, Sijia Hao, Lixia Wang, Zhifen Wang, Jinlong Han, Shuang Wu, Zhihui Hao

“…PR treatment suppressed upregulation of NOD-like receptor protein 3 (NLRP3), cysteinyl aspartate-specific proteases-1 (caspase-1), apoptosis-associated speck-like (ASC) and gasdermin D (GSDMD) at both the protein and mRNA expression levels. The addition of a small molecule dual-specifi city phosphatase inhibitor NSC 95397 inhibited the positive effects of PR. …”
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Effects of cold-pressed wheat germ oil and Bacillus subtilis on growth performance, digestibility, immune status, intestinal microbial enumeration, and gene expression of broilers... by Abdel-Moneim Eid Abdel-Moneim, Safaa A.M. Ali, M.G. Sallam, Ahmed M. Elbaz, Noura M. Mesalam, Zangabel S. Mohamed, AbdelRahman Y Abdelhady, Bing Yang, Mohamed Farouk Elsadek

“…Heat stress exposure adversely affected broiler growth performance, carcass traits, immune response, and insulin-like growth factor 1 (IGF-1) and mucin2 (MUC2) mRNA expression. However, the CWB group showed a lower FCR, reduced mortality rate, and increased BWG compared to the other groups. …”
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Protective Effect of Daikenchuto on Dextran Sulfate Sodium-Induced Colitis in Mice by Takaharu Matsunaga, Shinichi Hashimoto, Naoki Yamamoto, Ryo Kawasato, Tomohiro Shirasawa, Atsushi Goto, Koichi Fujisawa, Taro Takami, Takeshi Okamoto, Jun Nishikawa, Isao Sakaida

“…Colon length; histological scores; and interleukin- (IL-) 10, IL-1β, and tumor necrosis factor-α mRNA expression profiles were analyzed using real-time PCR. …”
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Integrin α8 is a useful cell surface marker of alveolar lipofibroblasts by Atsuki Fukada, Yasunori Enomoto, Ryo Horiguchi, Yoichiro Aoshima, Shiori Meguro, Hideya Kawasaki, Isao Kosugi, Tomoyuki Fujisawa, Noriyuki Enomoto, Naoki Inui, Takafumi Suda, Toshihide Iwashita

“…Compared to SCA-1+ fibroblasts, ITGA8+ fibroblasts showed higher mRNA expression of potential AT2-supportive factors, Fgf10, Fgf7, and Wnt2, but unexpectedly, exhibited lower efficiency in alveolar organoid formation. …”
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Effect of 25-hydroxycholecalciferol and high phytase doses on performance, vitamin D status, bone mineralization, and mechanistic target of rapamycin gene expression of broilers by L.S. Bassi, C.C.S. Martins, C.A. Lozano-Poveda, I.C. Dias, S.G. Oliveira, A. Maiorka

“…Phytase did not affect growth performance from 1 to 21 d, but a higher dose (2 000 FYT/kg) reduced feed intake and FCR from 22 to 42 d compared to 600 FYT/kg, also reducing FCR in the total period (P < 0.05). The mRNA expression of mechanistic target of rapamycin in breast muscle assessed at 42 d was enhanced with 2 000 FYT/kg (P < 0.001). …”
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Osteocalcin Alleviates Nonalcoholic Fatty Liver Disease in Mice through GPRC6A by Mingliang Zhang, Xiaomin Nie, Yeqing Yuan, Yansu Wang, Xiaojing Ma, Jun Yin, Yuqian Bao

“…Consistent with previous studies, uncarboxylated osteocalcin significantly protected high-fat diet-fed wild-type mice from obesity and NAFLD, while it did not protect high-fat diet-fed GPRC6ALKO mice from NAFLD. Differential mRNA expression of lipogenesis and lipolysis between GPRC6ALKO mice and control mice revealed that GPRC6A mediated the effects of osteocalcin in alleviating NAFLD through inhibiting lipid synthesis and promoting lipolysis. …”
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