Combination Fractional Carbon Dioxide Laser Treatment and Bone Marrow Mesenchymal Stem Cell Therapy Enhances the Treatment of Skin Photoaging in a Murine Model System by Li L, He Z, Yu C, Zhang C, Yu Y, Li Y, Xu X

“…In addition, immunohistochemical analyses and real-time polymerase chain reaction analyses were conducted to detect MMP-3 and MMP-9 expression.Results: After 1 week, both dermal thickness and collagen fiber density were significantly increased in the BMSC and combination treatment groups as compared to the control group (P< 0.05), while both of these parameters were significantly increased in all treatment groups after 4 weeks relative to the control group (P< 0.05), with the most pronounced effect in the combination therapy group (P< 0.05). MMP-3 and MMP-9 mRNA and protein levels in the treatment groups were decreased relative to the control group after 4 weeks.Conclusion: Combination BMSC and carbon dioxide laser therapy was more effective than either of these therapeutic approaches in isolation as a treatment for photoaged skin. …”
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MiR-127-3p靶向MAPK4对葡萄膜黑色素瘤细胞增殖、凋亡、迁移和侵袭的影响 by 魏丽, 连红梅, 刘鹏, 刘兴华, 吴书一, 刘萌萌

“…【方法】通过RT-qPCR检测人葡萄膜黑色素瘤组织及细胞、正常组织及细胞中miR-127-3p与MAPK4mRNA的表达；通过Lipofectamine2000说明书将mimic-NC、miR-127-3pmimic、pc-MAPK4质粒分别或联合转染进入SP6.5或OM431细胞；通过双荧光素酶报告检测miR-127-3p与MAPK4复染靶向关系；通过CCK-8法检测细胞增殖，流式细胞术检测细胞凋亡，划痕实验检测细胞迁移能力，Transwell法检测细胞侵袭能力，蛋白印迹法检测AKT/mTOR通路蛋白相对表达水平。…”
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Corneal protective effects of a new ophthalmic formulation based on vitamin B12 and sodium hyaluronate by Francesca Lazzara, Federica Conti, Grazia Maugeri, Velia D’Agata, Velia D’Agata, Ludovica Sotera, Claudio Bucolo, Claudio Bucolo

“…Cell viability, cytotoxicity, ROS production, and mRNA expression of pro-inflammatory cytokines (TNF-α and IL-1β) were assessed by MTT, LDH, 2′,7′–dichlorofluorescein diacetate (DCFDA) assays and Real-time PCR, respectively. …”
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Comprehensive pan-cancer analysis reveals NTN1 as an immune infiltrate risk factor and its potential prognostic value in SKCM by Fuxiang Luan, Yuying Cui, Ruizhe Huang, Zhuojie Yang, Shishi Qiao

“…Analysis of the TCGA, GTEx, and UALCAN databases revealed significant differences in NTN1 expression at both the mRNA and protein levels. Prognostic value was evaluated through univariate Cox regression and Kaplan–Meier methods. …”
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MTMR6 downregulation contributes to cisplatin resistance in oral squamous cell carcinoma by Kah Young Lee, Su Young Oh, Heon-Jin Lee, Tae-Geon Kwon, Jin-Wook Kim, Chang-Geol Shin, Su-Hyung Hong, So-Young Choi

“…Mouse xenografts derived from MTMR6-knockdown UMSCC1 cells exhibited increased resistance to cisplatin compared to controls. Conclusion Assessing mRNA levels of MTMR6 and has-miR-544a in biopsy samples could help predict cisplatin responsiveness in OSCC.…”
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整合素β4调控脑血管发育 by 邓昭华, 董影影, 李颍雯, 何欢欢

“…方法通过逆转录PCR（RT-PCR）检测靶向<italic>itgb4</italic> mRNA序列的吗啉环（MO）在斑马鱼胚胎中的敲低效果。利用体视显微镜和激光共聚焦显微镜（laser scanning confocal microscope， LSCM）观察抑制<italic>itgb4</italic>表达后，斑马鱼胚胎脑部血管的发育变化。…”
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TRPV Subfamily (TRPV2, TRPV3, TRPV4, TRPV5, and TRPV6) Gene and Protein Expression in Patients with Ulcerative Colitis by Joel J. Toledo Mauriño, Gabriela Fonseca-Camarillo, Janette Furuzawa-Carballeda, Rafael Barreto-Zuñiga, Braulio Martínez Benítez, Julio Granados, Jesus K. Yamamoto-Furusho

“…TRPV5 had significantly higher mRNA levels in a control group compared with active UC patients (P=0.02). …”
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Lignans from Patrinia scabiosaefolia improve insulin resistance by activating PI-3K/AKT pathway and promoting GLUT4 expression by Zhenhua Liu, Mengke Wang, Lijun Meng, Yixiao Chen, Qiuyi Wang, Yan Zhang, Xuefeng Xi, Wenyi Kang

“…Western blotting experiments showed that compounds 2 and 4 up-regulated the protein expressions of p-IRS, PI-3K, p-AKT and glucose transporter 4 (GLUT4), and promoted the transcription of GLUT4 mRNA. Therefore, the mechanisms of compounds 2 and 4 were presumed to improve IR by activating PI-3K/AKT signaling pathway.…”
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Phthalate exposure induces microRNA-5010/Nrf2-EGR1/GDF15 signaling expression in prostate cancer by Yuh-Shyan Tsai, Yeong-Chin Jou, Ian Seng Cheong, Hsiu-Ting Tung, Lin-Nei Hsu, Hsin-Tzu Tsai, Tzong-Shin Tzai

“…., SOD2, Heme oxygenase-1, and EGR-1) while increasing GDF15 mRNA expression via EGR-1 regulation in a dose- and time-dependent manner. …”
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High interstitial fluid pressure enhances USP1-dependent KIF11 protein stability to promote hepatocellular carcinoma progression by Zhengyi Wu, Chao Li, Shouhua Zhang, Liang Sun, Junwen Hu, Bingbing Qiu, Shuiqiu Liu, Yiran Hong, Tianxiang Chen, Kai Wang, Xiangbao Yin, Jinlong Yan

“…Omics analysis and subsequent molecular validation revealed that KIF11 protein levels were markedly upregulated under HIFP, despite no significant change in mRNA levels. Further investigation showed that this upregulation was linked to a reduction in KIF11's ubiquitin-mediated degradation, suggesting that the HIFP environment stabilizes KIF11 expression by inhibiting its degradation pathway. …”
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Integrated analysis of ATAC-seq and RNA-seq reveals ADSCP2 regulates oxidative phosphorylation pathway in hypertrophic scar fibroblasts by Qian Li, Zhe Quan, Ling Chen, Yiliang Yin, Xin Chen, Jingyun Li

“…Quantitative PCR analysis demonstrated that ADSCP2 treatment resulted in a reduction of COX6B1 and NDUFA1 mRNA expression levels. Furthermore, cellular ATP and lactic acid concentrations were diminished in the ADSCP2-treated group. …”
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Elevated miR-221-3p inhibits epithelial–mesenchymal transition and biochemical recurrence of prostate cancer via targeting KPNA2: an evidence-based and knowledge-guided strategy by Dingchao Li, Jingang Jian, Manhong Shi, Zihao Chen, Anguo Zhao, Xuedong Wei, Yuhua Huang, Yalan Chen, Jianquan Hou, Yuxin Lin

“…Then a knowledge-guided network strategy was applied to prioritize key targets of miR-221-3p in PCa progression based both on topological and functional characterization of genes in multi-omics miRNA-mRNA and protein-protein interaction networks. Finally, a key gene was computationally identified and experimentally validated using cell line and clinical samples through EdU assay, scratch assay, transwell assay, dual-luciferase reporter assay and the epithelial-to-mesenchymal transition (EMT)-related analysis. …”
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Exosomes from LPS-Stimulated hDPSCs Activated the Angiogenic Potential of HUVECs In Vitro by Xiangyu Huang, Wei Qiu, Yuhua Pan, Jianjia Li, Zhao Chen, Kaiying Zhang, Yifei Luo, Buling Wu, Wenan Xu

“…Compared to the uptake of N-EXO, uptake of LPS-EXO activated the angiogenic potential of HUVECs by promoting the proliferation, migration, and tube formation abilities in vitro. The mRNA expression levels of vascular endothelial growth factor (VEGF) and kinase-insert domain-containing receptor (KDR) in the LPS-EXO group were significantly higher than those in the N-EXO group. …”
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Taohong Siwu Decoction Exerts a Beneficial Effect on Cardiac Function by Possibly Improving the Microenvironment and Decreasing Mitochondrial Fission after Myocardial Infarction by Zhi-rong Luo, Han Li, Zhong-xin Xiao, Shui-jin Shao, Tian-tian Zhao, Yue Zhao, Fang-fang Mou, Bo Yu, Hai-dong Guo

“…The infarct size and collagen deposition in the infarct area were measured using Masson’s trichrome staining, and the number of CD31- and α-SMA-positive blood vessels in the peri-infarct and infarct area was evaluated by immunofluorescent staining. The mRNA expression of bFGF, IGF-1, and HGF was detected using RT-PCR assay. …”
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Structural characterization and anti-inflammatory activities of novel polysaccharides obtained from Pleurotus eryngii by Han Wang, Sai Ma, Alfred Mugambi Mariga, Qiuhui Hu, Qian Xu, Anxiang Su, Ning Ma, Gaoxing Ma

“…Biological activity analysis was then carried out and found that all these polysaccharides could significantly suppress the relative mRNA expression of toll-like receptor 4, nitric oxide (NO), tumor necrosis factor-α, interleukin (IL)-1β and IL-6 in lipopolysaccharide (LPS)-induced inflammation of RAW264.7 cells, as well as the over secretion of the above cell cytokines. …”
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Effects of lipid levels on growth performance and glucose and lipid metabolism of adult Nile tilapia (Oreochromis niloticus) fed with high carbohydrate diets by Ningning Xie, Jianmin Zhang, Ming Jiang, Xiaolin Meng, Lixue Dong, Xing Lu, Hua Wen, Juan Tian

“…Tilapia also showed an adaption to D1 and D2 diets with increased digestive enzyme activities and enhanced mRNA expression levels of glucose carriers. Furthermore, D1 and D2 diets influenced the glucose and lipid metabolism in muscle. …”
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纳米孔长读测序鉴定肝癌细胞中低氧特异性转录本 by 高力, 沈晟齐, 高平

“…结论纳米孔长读长测序技术的应用，不仅成功捕捉到了低氧条件下mRNA的特征性表达模式及特异性转录本，同时为深入探索低氧微环境下的转录组复杂性提供了强有力的技术支撑。…”
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The Fabrication and Evaluation of a Potential Biomaterial Produced with Stem Cell Sheet Technology for Future Regenerative Medicine by Shukui Zhou, Ying Wang, Kaile Zhang, Nailong Cao, Ranxing Yang, Jianwen Huang, Weixin Zhao, Mahbubur Rahman, Hong Liao, Qiang Fu

“…During 3 weeks after transplantation, the mRNA expression of cytokines, such as IL-4/IL-10, was obviously higher in decellularized ADSC sheets than that of porcine SIS. …”
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The cAMP Pathway Amplifies Early MyD88-Dependent and Type I Interferon-Independent LPS-Induced Interleukin-10 Expression in Mouse Macrophages by Orna Ernst, Yifat Glucksam-Galnoy, Muhammad Athamna, Iris Ben-Dror, Hadar Ben-Arosh, Galit Levy-Rimler, Iain D. C. Fraser, Tsaffrir Zor

“…We now show that the cAMP pathway directly upregulates IL-10 transcription and plays an important permissive and synergistic role in early, but not late, LPS-stimulated IL-10 mRNA and protein expression in mouse macrophages and in a mouse septic shock model. …”
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FAK抑制剂对肝癌HCC-LM3细胞骨架重排和侵袭的作用机制 by 姚红兵, 肖芳, 郭威, 吴嘉兴, 文雪霖, 蒋建晖, 华赟鹏

“…方法HCC-LM3细胞分为Control组、CT-707低剂量（1.5 μmol/L）组、CT-707中剂量（3 μmol/L）组、CT-707高剂量（6 μmol/L）组，采用Transwell小室实验、细胞划痕、MTT实验分别测定细胞侵袭、迁移能力及细胞活力；采用RT-qPCR和Western blot分别检测Palladin、Vimentin、MMP2和MMP9 mRNA及蛋白的表达；Western blot检测p-FAK、FAK、p-PI3K、PI3K、p-Akt及Akt蛋白的表达。…”
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