The Aryl Hydrocarbon Receptor (AhR) Is a Novel Gene Involved in Proper Physiological Functions of Pancreatic β-Cells by Shuhd Bin Eshaq, Jalal Taneera, Shabana Anjum, Abdul Khader Mohammed, Mohammad H. Semreen, Karem H. Alzoubi, Mohamed Eladl, Yasser Bustanji, Eman Abu-Gharbieh, Waseem El-Huneidi

“…Moreover, Ahr silencing led to the downregulation of major β-cell regulator genes, Ins1, Ins2, Pdx-1, and Glut2, at both the mRNA and protein levels. In summary, this study provides novel insights into the role of AhR in maintaining proper β-cell function. …”
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In Vivo Tracking of Chemokine Receptor CXCR4-Engineered Mesenchymal Stem Cell Migration by Optical Molecular Imaging by Senthilkumar Kalimuthu, Ji Min Oh, Prakash Gangadaran, Liya Zhu, Ho Won Lee, Ramya Lakshmi Rajendran, Se hwan Baek, Yong Hyun Jeon, Shin Young Jeong, Sang-Woo Lee, Jaetae Lee, Byeong-Cheol Ahn

“…MSCs were transduced with retroviruses to generate CXCR4-overexpressing MSCs (MSC-CXCR4/Fluc2) and MSCs (MSC/Fluc2). CXCR4 mRNA and protein expression was confirmed by RT-PCR and Western blotting, respectively, and it was higher in MSC-CXCR4/Fluc2 than in naive MSCs. eGFP expression was confirmed by confocal microscopy. …”
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Biglycan-driven risk stratification in ZFTA-RELA fusion supratentorial ependymomas through transcriptome profiling by Konstantin Okonechnikov, David R. Ghasemi, Daniel Schrimpf, Svenja Tonn, Martin Mynarek, Jan Koster, Till Milde, Tuyu Zheng, Philipp Sievers, Felix Sahm, David T.W. Jones, Andreas von Deimling, Stefan M. Pfister, Marcel Kool, Kristian W. Pajtler, Andrey Korshunov

“…Our results indicate that integrating BGN expression (at mRNA and/or protein level) into risk stratification models may improve ST-EPN ZFTA-RELA outcome prediction. …”
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Development of Collagen-Based 3D Matrix for Gastrointestinal Tract-Derived Organoid Culture by Joo Hyun Jee, Dong Hyeon Lee, Jisu Ko, Soojung Hahn, Sang Yun Jeong, Han Kyung Kim, Enoch Park, Seon Young Choi, Sukin Jeong, Joong Woon Lee, Han-Jun Cho, Min-Soo Kwon, Jongman Yoo

“…Then, characteristics of mouse colon organoids were analyzed by morphology and quantitative messenger RNA (mRNA) expression, revealing that the mouse colon organoids grown in the collagen-based matrix and in Matrigel had quite similar morphology, specific markers, and proliferative rates. …”
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Extracellular Vesicles from Amnion-Derived Mesenchymal Stem Cells Ameliorate Hepatic Inflammation and Fibrosis in Rats by Masatsugu Ohara, Shunsuke Ohnishi, Hidetaka Hosono, Koji Yamamoto, Kohei Yuyama, Hideki Nakamura, Qingjie Fu, Osamu Maehara, Goki Suda, Naoya Sakamoto

“…AMSC-EVs significantly decreased the number of Kupffer cells (KCs) in the liver of rats with NASH and the mRNA expression levels of inflammatory cytokines such as tumor necrosis factor- (Tnf-) α, interleukin- (Il-) 1β and Il-6, and transforming growth factor- (Tgf-) β. …”
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The transcriptional regulation effects of histidine, isoleucine and glutamate on free exopolysaccharide biosynthesis of Streptococcus thermophilus 937 by Yunchao Wa, Yunchao Wa, Xia Zhao, Xia Zhao, Chenchen Zhang, Chenchen Zhang, Hengxian Qu, Hengxian Qu, Dawei Chen, Dawei Chen, Xia Chen, Xia Chen, Yujun Huang, Yujun Huang, Ruixia Gu, Ruixia Gu

“…Our previous studies showed f-EPS production from S. thermophilus 937 was increased 2-fold in the presence of 15 mM of glutamate, isoleucine, and histidine in the chemically defined medium.MethodsIn this study, we used transcriptomics and qPCR to further explore the specific mechanism of the enhanced effect of 3 amino acids on the f-EPS biosynthesis of S. thermophilus 937.ResultsThe mRNA-seq analysis and targeted pathway analysis indicated that genes associated with histidine/valine/leucine/ isoleucine/phenylalanine/tyrosine/tryptophan synthesis, galactose metabolism, purine metabolism and quorum sensing in S. thermophilus 937 were significantly upregulated under increasing concentrations of histidine, isoleucine and glutamate in chemically defined medium (CDM). qPCR results showed that the significant upregulation of galactose metabolism- and nucleotide sugar synthesis-related genes was attributed to the increase in glutamate concentration, and glutamate could induce the expression of galR. …”
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间充质干细胞抑制MEK/ERK信号通路改善盲肠结扎穿孔所致急性肺损伤中肺泡Ⅱ型上皮细胞的凋亡

“…肺组织fas?bax/bcl-2及caspase3 mRNA扩增倍数CLP/Saline vs CLP/MSC p值分别为0.09?0.004及0.037,均 < 0.05,蛋白水平fas?…”
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Drosophila ubiquitin-specific peptidase 14 stabilizes the PERIOD protein by regulating a ubiquitin ligase SLIMB by So Who Kang, Jung-Eun Park, Soonhyuck Ok, Minhui Um, Hyeonjeong Son, Seunghee Byun, Nayoung Park, Su Jin Lee, Thị Xuân Thùy Trần, Gyeongmin Kim, Jeonghun Yeom, Kyunggon Kim, Eun Young Kim, Min-Ji Kang

“…We find that overexpression of USP14 in clock cells reduces PER protein levels without altering its mRNA levels whereas USP14 knockdown increases PER protein levels, suggesting that USP14 regulates PER post-translationally. …”
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Antiviral Activity and Underlying Mechanism of <i>Moslae herba</i> Aqueous Extract for Treating SARS-CoV-2 by Yan Feng, Qiong Ge, Jian Gao, Zhuoying Wu, Yunyi Zhang, Haiyan Mao, Beibei Wu, Changping Xu

“…Of the nine, the PNP and TPI1 were identified as core targets as AEMH treatment significantly regulated the mRNA expression level of the two genes on infected cells. …”
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Anti-inflammatory treatment using alpha melanocyte stimulating hormone (α-MSH) does not alter osteoblasts differentiation and fracture healing by Johanna Graue, Melanie Timmen, Katharina Schmitz, Daniel Kronenberg, Markus Böhm, Kishor K. Sivaraj, M. Gabriele Bixel, Richard Stange

“…Using quantitative real time PCR, we analyzed mRNA expression of receptors. To explore the impact of α-MSH on bone regeneration in vivo, treatment with α-MSH or NaCl (control) was performed in a murine fracture-healing model using a closed femur fracture stabilized with an intramedullary implant (female, n = 6–8 mice per group). …”
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Modulation of Innate Immunity by G-CSF and Inflammatory Response by LBPK95A Improves the Outcome of Sepsis in a Rat Model by Haoshu Fang, Chuanfeng Hua, Stefanie Weiss, Anding Liu, Wenhui Cheng, Ralf Claus, Jürgen Rödel, Olaf Dirsch, Uta Dahmen

“…Irrespective of dose, the blockade of the LPS-LBP interaction was associated with low systemic cytokine levels and delayed increases in hepatic TNF-α and IL-6 mRNA expression. The delayed increase in cytokines was associated with the phosphorylation of STAT3 and AKT. …”
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Attenuated titin protein expression is associated with advanced stages of ovarian cancer by Harvey Sharma, Jaskaran Aujla, Asad Nawaz, Thabet Khasawneh, Ayesha Alvero, Robert T. Morris, Asma Basha, Laila Tutunji, Toshima Z. Parris, Khalil Helou, Ghassan M. Saed

“…Quantitative real-time PCR analyzed titin mRNA in cell lines, including chemosensitive, chemoresistant, and normal ovarian cells. …”
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Time-Qualified Patterns of Variation of PPARγ, DNMT1, and DNMT3B Expression in Pancreatic Cancer Cell Lines by Valerio Pazienza, Francesca Tavano, Massimo Francavilla, Andrea Fontana, Fabio Pellegrini, Giorgia Benegiamo, Vincenzo Corbo, Fabio Francesco di Mola, Pierluigi Di Sebastiano, Angelo Andriulli, Gianluigi Mazzoccoli

“…We investigated the time-related patterns of PPARG, DNMT1, and DNMT3B expression monitoring their mRNA levels by qRT-PCR at different time points over a 28-hour span in BxPC-3, CFPAC-1, PANC-1, and MIAPaCa-2 PC cells after synchronization with serum shock. …”
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N88S seipin-related seipinopathy is a lipidopathy associated with loss of iron homeostasis by Mariana O. Ribeiro, Mafalda Oliveira, Verónica Nogueira, Vítor Costa, Vitor Teixeira

“…This was linked to changes in the expression of Aft1p-controlled iron regulon genes, including the mRNA-binding protein CTH2 and the high-affinity iron transport system member FET3, in a p38/Hog1p- and Msn2p/Msn4p-dependent manner. …”
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Modulation of the endocannabinoid system by (S)-ketamine in an animal model of depression by Nicole R. Silva, Shokouh Arjmand, Luana B. Domingos, Adriano M. Chaves-Filho, Melina Mottin, Caroline C. Real, Anna L. Waszkiewicz, Pedro H. Gobira, Alessio Nicola Ferraro, Anne M. Landau, Carolina H. Andrade, Heidi K. Müller, Gregers Wegener, Sâmia R.L. Joca

“…Changes in eCB receptors and enzymes were assessed at mRNA and protein levels (qPCR and western blot), CB1 binding ([3H]SR141716A autoradiography) and endocannabinoid content (lipidomics). …”
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Influence of Titanium Alloy Scaffolds on Enzymatic Defense against Oxidative Stress and Bone Marrow Cell Differentiation by Lais Morandini Rodrigues, Elis Andrade Lima Zutin, Elisa Mattias Sartori, Daniela Baccelli Silveira Mendonça, Gustavo Mendonça, Yasmin Rodarte Carvalho, Luana Marotta Reis de Vasconcellos

“…At day 14, all scaffolds had more than 2-fold induction for Runx2 mRNA levels, with statistically significant differences compared to the control group. …”
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Decreased Frequencies of Th17 and Tc17 Cells in Patients Infected with Avian Influenza A (H7N9) Virus by Jiaqi Bao, Dawei Cui, Xiaochen Wang, Qianda Zou, Dejian Zhao, Shufa Zheng, Fei Yu, Li Huang, Yuejiao Dong, Xianzhi Yang, Guoliang Xie, Weizhen Chen, Yu Chen

“…In this study, we found that the frequencies of Th17 and Tc17 cells among human peripheral blood mononuclear cells (PBMCs) as well as IL-17A protein and mRNA levels were markedly decreased in patients with acute H7N9 virus infection. …”
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Transcriptome analysis reveals modulations in glycosylation profiles of the mucosal barrier and their potential interaction with gut microbiota in weaned piglets by Hao Cheng, Hao Li, Yujie Zhao, Kai Yang, Jing Wang, Bie Tan, Xiaokang Ma

“…DLY piglets displayed elevated mRNA expression levels of MUC2 and MUC5AC in colonic mucosal tissue than NX piglets (P < 0.05). …”
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Serological insights from SARS-CoV-2 heterologous prime and boost responses in Thailand by Daniel Ward, Lapasrada Pattarapreeyakul, Rujiraporn Pitaksalee, Naphatcha Thawong, Waritta Sawaengdee, Suthida Tuntigumthon, Catriona Patterson, Kevin Tetteh, Susana Campino, Panadda Dhepakson, Surakameth Mahasirimongkol, Taane G. Clark

“…The Thailand Ministry of Health adopted these strategies using vector, inactivated, and mRNA vaccines. However, this approach has introduced challenges for SARS-CoV-2 sero-epidemiology studies. …”
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Case report: Deciphering the clinical significance of a novel partial BRCA1 exon 10 duplication in a patient with triple-negative breast cancer by Alice Faversani, Debora Manuelli, Davide Barteselli, Giulia Melloni, Carlo Santaniello, Luigi Corsaro, Luigi Corsaro, Davide Sacco, Davide Sacco, Davide Clerici, Laura Gargiulo, Fulvio Ferrara, Lucy Costantino

“…RNA analysis allowed to confirm the production of an altered mRNA showing the duplicated sequence. In this way, we were able to assign a clinical significance to the new alteration and classify it as a pathogenic variant. …”
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