Creatine monohydrate administration delayed muscle glycolysis of antemortem-stressed broilers by enhancing muscle energy status, increasing antioxidant capacity and regulating musc... by Ning Liu, Qingzhen Zhong, Zewei Sun, Bolin Zhang

“…However, CMH administration ameliorated energy status, delayed muscle glycolysis, elevated mRNA expression involved in Cr metabolism and inhibited AMPK signaling of broilers experienced preslaughter transport stress. …”
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Preassembled complexes of hAgo2 and ssRNA delivered by nanoparticles: a novel silencing gene expression approach overcoming the absence of the canonical pathway of siRNA processing... by Shimaa A. E-S. El-Sayed, Mohamed A. Rizk, Hang Li, Uday Kumar Mohanta, Iqra Zafar, Shengwei Ji, Zhuowei Ma, Thom Do, Yongchang Li, Daisuke Kondoh, Jerzy Jaroszewski, Xuenan Xuan

“…We demonstrated a reduction in the expression of target genes at the mRNA level. Furthermore, this silencing inhibited Babesia growth in vitro and in vivo. …”
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Qinggan Yipi capsule ameliorates hepatic fibrosis in rats by down-regulating the TGF-β1/Smad2/3 signaling pathway and improving gut microbiota imbalance by Wenjing Xue, Haiqing Liu, Ziheng Su, Siqi Wang, Junping Cheng, Yunzhi Pan, Lurong Zhang

“…In addition, the results of qPCR and Western blot analysis from both in vitro and in vivo experiments indicated that QgYp significantly downregulated the expression of proteins and mRNA associated with the TGF-β1/Smad2/3 pathway. …”
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Knockdown ATG5 gene by rAAV9 alleviates doxorubicin-induced cardiac toxicity by inhibiting GATA4 autophagic degradation by Ai-Li Xu, Zheng Shen, Shi-Hao Wang, Hai-Yun Luan, Yong Xu, Ze-Chun Kang, Zi-Qi Liao, Jie Liu, Xiao-Lei Duan, Wei-Hua Bian, Hui Sun, Xin Xie

“…Heart function was determined using echocardiography, and cardiac tissue was assessed for protein expression, mRNA levels, fibrosis, and immunofluorescent staining. …”
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Eugenol inhibits NEAT1 as a ceRNA in pre-cancerous breast lesions by Yusheng Liu, Guijuan Zhang, Yi Ma, Min Ma, Xuefeng Jiang

“…Further, we established a lncRNA-miRNA-mRNA ceRNA network through bioinformatics analysis and investigated mechanistic roles of lncRNAs as ceRNAs and the anti-tumor effect of EU using MCF-10AT cells in vitro as well as PBL model rats in vivo. …”
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A novel histone acetylation-associated gene signature with prognostic value in Ewing sarcoma by Anshun Wu, Fayin Liu, Lei Zhou, Runyi Jiang, Shangjiang Yu, Zihuan Zhou, Qi Zhang, Qian Zhang, Dongjie Jiang, Shaohui He, Haifeng Wei

“…After collecting EWS patients with mRNA sequencing data from the Gene Expression Omnibus (GEO) database and a list of HARGs from previous studies, Cox regression and Least Absolute Shrinkage and Selection Operator (LASSO) regression were performed to construct a prognostic gene signature based on HARGs. …”
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Molecular profiling of bladder cancer xenografts defines relevant molecular subtypes and provides a resource for biomarker discovery. by Sharada Mokkapati, Ganiraju Manyam, Alexis R. Steinmetz, Côme Tholomier, Alberto Martini, Woonyoung Choi, Bogdon Czerniak, Byron H Lee, Colin P Dinney, David J McConkey

“…Tumor growth was monitored using bioluminescence imaging and mRNA-based molecular classification was used to characterize xenografts into molecular subtypes. …”
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Diethylcarbamazine Reduces Chronic Inflammation and Fibrosis in Carbon Tetrachloride- (CCl4-) Induced Liver Injury in Mice by Sura Wanessa Santos Rocha, Maria Eduarda Rocha de França, Gabriel Barros Rodrigues, Karla Patrícia Sousa Barbosa, Ana Karolina Santana Nunes, André Filipe Pastor, Anne Gabrielle Vasconcelos Oliveira, Wilma Helena Oliveira, Rayana Leal Almeida Luna, Christina Alves Peixoto

“…The reduction of hepatic injury in the DEC-treated group was confirmed by the COX-2 and iNOS mRNA expression levels. Based on the results of the present study, DEC can be used as a potential anti-inflammatory drug for chronic hepatic inflammation.…”
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Group 2 Innate Lymphoid Cells Participate in Renal Fibrosis in Diabetic Kidney Disease Partly via TGF-β1 Signal Pathway by Cuiping Liu, Ludan Qin, Jingya Ding, Luping Zhou, Chenlin Gao, Ting Zhang, Man Guo, Wei Huang, Zongzhe Jiang, Yang Long, Yong Xu

“…Moreover, the IL-4Rα and IL-13Rα1 mRNA in HK2 cells were increased followed by high glucose alone or combined with IL-4 or IL-13, but the differences were not statistically significant (P>0.05). …”
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LncRNAs in serum-derived extracellular vesicles are potential biomarker and correlated with immune infiltration in gastric cancer by Juan Ding, Yunyan Teng, Rongshu Cui, Jin Liu, Ke Xiao, Zhaogang Dong, Yi Zhang, Xiaofei Xu

“…Finally, the lncRNA-miRNA-mRNA network showed that three lncRNAs were predicted to interact with 15 miRNAs and 69 mRNAs. …”
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IL-33 Mediates Lung Inflammation by the IL-6-Type Cytokine Oncostatin M by Fernando Botelho, Anisha Dubey, Ehab A. Ayaub, Rex Park, Ashley Yip, Allison Humbles, Roland Kolbeck, Carl D. Richards

“…Adenoviral OSM (AdOSM) markedly induced IL-33 mRNA and protein levels in wild-type animals while IL-33 was undetectable in IL-33-/- animals. …”
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C2C12成肌细胞体外诱导分化为肌管的实验 by 陈永乐, 周光前, 邓宇斌, 智伟, 谢彗琪, 邓力, 杨志明, 王亚柱

“…③20 mL/L马血清诱导C 2C 12细胞向成熟肌细胞分化过程中，肌相关蛋白分子表达发生变化：结蛋白（desmin）、成肌分化抗原（MyoD）、生肌素（myogenin）和肌球蛋白（myosin）等表达逐渐增强（未刺激时分别约为49．6％ ± 1．1％、23．4％ ± 1．1％、4．8％ ± 1．6％和2．6％ ± 1．5％；第6天时分别提高为80．4％ ± 1．8％、85．4％ ± 1．1％、22．2％ ± 1．1％和26．0％ ± 1．6％；P<0．001）；desmin、MyoD和myogenin相应的mRNA分子也可以通过RT-PCR检测到。【结论】 ①低浓度的马血清能够有效刺激C 2C 12成肌细胞向成熟细胞分化，以20 mL/L浓度效果最好；②C 2C 12细胞是研究肌细胞发育分化的理想模板；③肌分化发育过程比较复杂，许多因素都可能起影响，研究设计要尽量标准化。…”
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High serum CA19-9 predicts severe cholecystitis in calculous cholecystitis patients by Shouwen Wang, Dachen Zhou, Wanjin Chen, Qi Guo, Liujin Hou, Ruolin Wu, Wei Wang, Muhammad Annus Khan, Muhammad Ahmad, Fan Huang, Meijuan Zheng, Guobin Wang, Hongchuan Zhao, Xiaoping Geng, Xiaojun Yu

“…Besides, the gallbladder specimens were collected for immunohistochemical staining and mRNA sequencing. Results Abdominal pain occur in more than 90% patients in high group, which is similar with that of normal group. …”
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Development of highly bioactive long-acting recombinant porcine FSH for batch production management of sows by Jiawei Zhang, Lei Shi, Xiaorong Zhong, Yunhe Bai, Jun Dou, Lu Zhang, Xiaochao Shi, Bingchun Wu, Zemin Tan, Lin Yan, Jinku Yu, Jingru Zhang, Guo Han, Jiansheng Xiong, Hua Zhang, Xingyuan Cao, Haoshu Luo

“…Through ovarian weight gain, superovulation and fertility testing assays, our results revealed that a single rpFSH-pFc treatment could effectively promote follicular development and maturation in vivo. Meanwhile, the mRNA expression levels of the target genes associated with follicular development, maturation and ovulation were significantly up-regulated after rpFSH-pFc treatment. …”
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Gas-phase fractionation DDA promotes in-depth DIA phosphoproteome analysis by Zhiwei Tu, Yabin Li, Shuhui Ji, Shanshan Wang, Rui Zhou, Gertjan Kramer, Yu Cui, Fei Xie

“…The differentially expressed phosphosites (DEPsites) in direct ALI were found in proteins related to mRNA processing and RNA. DEPsites in indirect ALI were enriched in proteins related to microtubule polymerization, positive regulation of microtubule polymerization and fibroblast migration. …”
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Genetic Polymorphisms of Toll-like receptors 2 and 9 as Susceptibility Factors for the Development of Ankylosing Spondylitis and Psoriatic Arthritis by Camila F. Oliveira-Toré, Amarilis G. Moraes, Gabriela F. Martinez, Janisleya S. F. Neves, Luciana C. Macedo, Marco A. Rocha-Loures, Marília G. Quirino, Hugo V. Alves, Ana M. Sell, Jeane E. L. Visentainer

“…Polymorphisms in TLR genes that lead to changes in these receptors or that interfere with the transcription rates of mRNA TLR may be involved in the chronic inflammatory immune response observed in SpA. …”
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Cellular heterogeneity and cytokine signatures in acute myeloid leukemia: A novel prognostic model by Jinxia Cao, Bin Hu, Tianqi Li, Dan Fang, ling Jiang, Jun Wang

“…Leveraging this comprehensive dataset, we constructed a prognostic risk score model based on seven pivotal cytokine-related genes: CCL23, IL2RA, IL3RA, IL6R, INHBA, TNFSF15, and TNFSF18. The mRNA levels of 7 genes in the risk score model have significant different. …”
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Identification of Anoikis-Related Genes in Chronic Kidney Disease Based on Bioinformatics Analysis Combined with Experimental Validation by Liu H, Mei M, Zhong H, Lin S, Luo J, Huang S, Zhou J

“…Among the 4 types of CKD mouse models, the mRNA expression levels of LAMB3 and CDH3 were significantly (P< 0.05) up-regulated in the model group.Conclusion: We identified 6 hub genes that may serve as potential key biomarkers of Anoikis-related progression in CKD.Keywords: chronic kidney disease, bioinformatics analysis, anoikis, biomarkers…”
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Retinoic Acid Signal Negatively Regulates Osteo/Odontogenic Differentiation of Dental Pulp Stem Cells by Jiangyi Wang, Guoqing Li, Lei Hu, Fei Yan, Bin Zhao, Xiaoshan Wu, Chunmei Zhang, Jinsong Wang, Juan Du, Songlin Wang

“…Activation of RA signal in vitro inhibited ALP activities and mineralization of human DPSCs and decreased the mRNA expression of ALP, osteocalcin, osteopontin, and a transcription factor, osterix. …”
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Exploring the functional role of POU5F1 using CRISPR RNP electroporation of zygote (CRISPR-EZ) in buffaloes by Meeti Punetha, Dharmendra Kumar, Suman Choudhary, Sheetal Saini, Kamlesh Kumari Bajwa, Surabhi Sharma, Manu Mangal, P. S. Yadav, T. K. Datta

“…We targeted the buffalo POU5F1 gene using the abovementioned parameters, which caused nonsense-mediated mRNA decay and led to complete knockout (KO) of the POU5F1 gene. …”
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