The Mycoplasma hyopneumoniae protein Mhp274 elicits mucosal and systemic immune responses in mice by Mengqi Xie, Zhongshun Huang, Yun Zhang, Yujie Gan, Huiying Li, Dan Li, Honglei Ding

“…The levels of IFN-γ, IL-4, and IL-17, in the supernatants of splenocytes and in serum were measured, and the mRNA levels of three cytokines in splenocytes were analyzed. …”
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Nephropathy in Hypertensive Animals Is Linked to M2 Macrophages and Increased Expression of the YM1/Chi3l3 Protein by Paula Andréa Malveira Cavalcante, Natalia Alenina, Alexandre Budu, Leandro Ceotto Freitas-Lima, Thaís Alves-Silva, Juan Sebastian Henao Agudelo, Fatimunnisa Qadri, Niels Olsen Saraiva Camara, Michael Bader, Ronaldo Carvalho Araújo

“…We examined the kidneys of TGM123-FVB/N animals to assess macrophage polarization and end-organ damage. mRNA expression was evaluated using real-time PCR, and protein levels were assessed through ELISA, CBA, Western blot, and immunofluorescence. …”
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Inhibition of complement system-related gene ITGB2 attenuates epithelial–mesenchymal transition and inflammation in diabetic nephropathy by Jun Peng, Wenqi Zhao, Lu Zhou, Kun Ding

“…Also, VWF and ITGB2 were significantly related to the clinical features, including age, serum creatinine level, and GFR (MDRD). Besides, mRNA and protein expression levels of ITGB2 in HG-treated HK-2 cells were remarkably elevated. …”
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Selective suppression of influenza A/H5N1 virus replication <i>in vitro</i> using nanocomplexes consisting of siRNA and aminopropylsilanol nanoparticles by Marina N. Repkova, Asya S. Levina, Oleg Yu. Mazurkov, Elena V. Makarevich, Ekaterina I. Filippova, Natalya A. Mazurkova, Valentina F. Zarytova

“…The most effective nanocomplex that contained siRNA targeted at the chosen region of mRNA segment 5 of the viral genome reduced virus replication in the culture by a factor of 630. …”
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Livogrit mitigates ANIT-induced cholestasis-like symptoms in an in vivo model by curbing hepatic inflammation and regulating BAX, TGF-β, MMP-9 and α-SMA gene expression by Acharya Balkrishna, Ritu Paliwal, Surjeet Singh, Rani Singh, Vivek Gohel, Rishabh Dev, Kunal Bhattacharya, Sandeep Sinha, Anurag Varshney

“…At molecular level, Livogrit inhibited up-regulation of BAX, TGF-β, α-SMA, and MMP-9 mRNA expressions, associated with the liver damages. …”
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Altered CD73-Adenosine Signaling Linked to Infection in Patients undergoing hemodialysis by Xiang F, Zhang Z, Nie Y, Cao X, Li Y, Chen X, Gong S, Zou J, Teng J, Ding X, Shen B

“…Animal models demonstrated reduced CD73 mRNA transcript and adenosine receptor levels, along with decreased serum adenosine levels in CKD. …”
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Nicotine-induced CHRNA5 activation modulates CES1 expression, impacting head and neck squamous cell carcinoma recurrence and metastasis via MEK/ERK pathway by Chen Feng, Wei Mao, Chenyang Yuan, Pin Dong, Yuying Liu

“…Knockdown of CHRNA5 can reduce cell migration, invasion, and proliferation, whereas nicotine exposure can reverse this trend. Additionally, the mRNA and protein expression of CES1 decreases with the knockdown of CHRNA5, indicating a regulatory relationship between the two. …”
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Analysis of the structure and function of the tomato <i>Solanum lycopersicum</i> L. MADS-box gene <i>SlMADS5</i> by A. V. Nezhdanova, M. A. Slugina, E. A. Dyachenko, A. M. Kamionskaya, E. Z. Kochieva, A. V. Shchennikova

“…Analysis of the expression of SlMADS5 and the tobacco genes NtLFY, NtAP1, NtWUS, NtAG, NtPLE, NtSEP1, NtSEP2, and NtSEP3 in leaves and apexes of transgenic and control plants showed that SlMADS5 mRNA is present only in tissues of transgenic lines. …”
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Studies on the alleviating effect of Bifidobacterium lactis V9 on dextran sodium sulfate-induced colitis in mice by Xiaoyan Duan, Xiaoyan Duan, Xiaoyan Duan, Xiaoyan Duan, Rilige Wu, Jianbo Li, Jianbo Li, Zeya Li, Yanqi Liu, Ping Chen, Bangmao Wang, Bangmao Wang, Bangmao Wang

“…RT-qPCR and Western blotting were used to detect mRNA and protein expression of target genes. The fecal microbiota was analyzed by 16S rRNA sequencing. …”
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Targeting EP2 Receptor Improves Muscle and Bone Health in Dystrophin^−/−/Utrophin^−/− Double-Knockout Mice by Xueqin Gao, Yan Cui, Greg Zhang, Joseph J. Ruzbarsky, Bing Wang, Jonathan E. Layne, Xiang Xiao, Johnny Huard

“…We found that Ep2, Ep4, Cox-2, 15-Pgdh mRNA, and PGE2 were significantly increased in DKO-Hom mice compared to wild-type (WT) mice. …”
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Therapeutic target genes and regulatory networks of gallic acid in cervical cancer by Zhixi You, Ye Lei, Yongkang Yang, Zhihui Zhou, Xu Chao, Keyi Ju, Songyi Wang, Yuanyuan Li, Yuanyuan Li

“…The upstream 13 miRNAs, 4 lncRNA, and 42 circRNAs were detected and used to construct a lncRNA/circRNA-miRNA-mRNA-pathway regulatory network.ConclusionThis study identified candidate genes and the regulatory networks underlying the therapeutic effects of GA on CC using GA data mining methods, thus establishing a theoretical basis for targeted therapy of CC.…”
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High Glucose and Lipopolysaccharide Prime NLRP3 Inflammasome via ROS/TXNIP Pathway in Mesangial Cells by Hong Feng, Junling Gu, Fang Gou, Wei Huang, Chenlin Gao, Guo Chen, Yang Long, Xueqin Zhou, Maojun Yang, Shuang Liu, Shishi Lü, Qiaoyan Luo, Yong Xu

“…We found that the expression of thioredoxin-interacting protein (TXNIP), NLRP3, and IL-1β was observed by immunohistochemistry in vivo. Simultaneously, the mRNA and protein levels of TXNIP, NLRP3, procaspase-1, and IL-1β were significantly induced by high glucose concentration and lipopolysaccharide in a dose-dependent and time-dependent manner in vitro. …”
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The total extract of Abelmoschus manihot (L.) medic flowers (TEA) mediated Nrf2-TFAM signalling to regulate mitochondrial antioxidant mechanism by Ying Song, Xinyi Zhu, Beibei Wang, Qisong Li, Biwei Song

“…RT-PCR was used to detect mRNA and mtDNA expression. The expression of the target protein was detected by Western blotting and immunofluorescence. …”
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Identification of SETD4 as an Onco‐Immunological Biomarker Encompassing the Tumor Microenvironment, Prognoses, and Therapeutic Responses in Various Human Cancers by Yuyun Zhong, Ruiqi Wang, Zijie Huang, Zhaoting Hu, Bin Peng, Bin Chen, Liyue Sun

“…The analysis revealed that the mRNA level of SETD4 was modulated by promoter methylation, and patients with lower methylation levels showed shorter survival times. …”
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Dairy Foods in a Moderate Energy Restricted Diet Do Not Enhance Central Fat, Weight, and Intra-Abdominal Adipose Tissue Losses nor Reduce Adipocyte Size or Inflammatory Markers in... by Marta D. Van Loan, Nancy L. Keim, Sean H. Adams, Elaine Souza, Leslie R. Woodhouse, Anthony Thomas, Megan Witbracht, Erik R. Gertz, Brian Piccolo, Andrew A. Bremer, Michael Spurlock

“…No diet differences were observed in weight, fat, or IAAT loss; nor SAT mRNA expression of inflammation, circulating cytokines, fasting lipids, glucose, or insulin. …”
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Increased TLR4 Expression Aggravates Sepsis by Promoting IFN-γ Expression in CD38−/− Mice by Qi Li, Chenyi Wu, Zhenlong Liu, Huiqing Zhang, Yuna Du, Yuxiang Liu, Kuangyu Song, Qiaofa Shi, Rong Li

“…In addition, compared with CD38−/−TLR4mut mice, we found an increase of TLR4 expression and mRNA expression of these cytokines in the kidney of CD38−/− mice, although only increased IFN-γ level was detected in the serum. …”
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hnRNPU-mediated pathogenic alternative splicing drives gastric cancer progression by Guoguo Jin, Yanming Song, Shaobo Fang, Mingyang Yan, Zhaojie Yang, Yang Shao, Kexin Zhao, Meng Liu, Zhenwei Wang, Zhiping Guo, Zigang Dong

“…This demethylation enhances the stability of hnRNPU mRNA, consequently promoting alternative splicing of MET. …”
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Anti-Inflammatory and Anti-Migratory Effects of Morin on Non-Small-Cell Lung Cancer Metastasis via Inhibition of NLRP3/MAPK Signaling Pathway by Punnida Arjsri, Kamonwan Srisawad, Sonthaya Umsumarng, Pilaiporn Thippraphan, Songyot Anuchapreeda, Pornngarm Dejkriengkraikul

“…Morin downregulated invasive proteins (MMP-2, MMP-9, u-PAR, u-PA, MT1-MMP) and EMT markers (fibronectin, N-cadherin, vimentin) (<i>p</i> < 0.01) while also reducing the mRNA levels of NLRP3, IL-1β, IL-18, and IL-6. Mechanistic investigations revealed that morin suppressed NLRP3 inflammasome activity and inactivated MAPK pathways. …”
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NAD⁺激活SIRT3/IDH2信号通路改善高血压血管内皮功能障碍 by 丘雨旻, 陈曦, 张建宁, 刘张弛, 朱秋霞, 张美信, 陶军, 吴杏

“…采用迁移实验和划痕实验评估体外内皮细胞功能；采用免疫荧光染色法检测mtROS水平；采用RT-PCR检测mtDNA、SIRT3、异柠檬酸脱氢酶2（IDH2）的mRNA表达；采用酶联免疫吸附法检测8-羟基-2'-脱氧鸟苷的水平；采用Western blot法检测p-eNOS、eNOS、SIRT3、IDH2蛋白表达水平。…”
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Deciphering the potential ability of DExD/H-box helicase 60 (DDX60) on the proliferation, diagnostic and prognostic biomarker in pancreatic cancer: a research based on silico, RNA-... by Dongdong Zhang, Enze Zhang, Ying Cai, Yixin sun, Peiji Zeng, Xiaohua Jiang, Yifan Lian

“…And is associated with poorer outcomes for pancreatic patients. The mRNA expression level of DDX60 was correlated with lymph node metastasis and grade in clinical pancreatic patients. …”
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