Construction of a Novel Necroptosis-Related Signature in Rat DRG for Neuropathic Pain by Liu Y, Hao S, Hao H, Zheng G, Bing J, Kang L, Li J, Zhao H, Hao H

“…This study aims to identify a gene signature related to necroptosis in DRG that can predict neuropathic pain.Methods: The mRNA expression profiles associated with neuropathic pain (GSE24982 and GSE30691) were acquired from the Gene Expression Omnibus (GEO) database. …”
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Identification and the molecular mechanism of novel duck liver-derived anti-inflammatory peptides in lipopolysaccharide-induced RAW264.7 cell model by Xiankang Fan, Laidi Zhang, Yangying Sun, Changyu Zhou, Qiang Xia, Lihui Du, Zhen Wu, Daodong Pan

“…In the lipopolysaccharide-induced RAW264.7 cell model, the release of NO, TNF-α, and IL-6 and the mRNA expression of inflammatory factors (TNF-α, IL-6, COX-2, and NF-κB) were significantly inhibited by these peptides. …”
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RETRACTED ARTICLE: NR3C2 affects the proliferation and invasiveness of colon cancer cells through the Wnt/β-Catenin signaling pathway by Ke Nie, Zhong-Jiang He, Ling-Jun Kong

“…Abstract Purpose The aim of this study was to explore the potential correlation between the nuclear receptor subfamily 3 group C member 2 (NR3C2) and outcomes of colon cancer, along with the mechanisms underlying this association. Method mRNA (messenger RNA) data and clinical records pertaining to colon cancer were retrieved from The Cancer Genome Atlas (TCGA) database. …”
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Comprehensive Analysis of Competing Endogenous RNA Network Focusing on Long Noncoding RNA Involved in Cirrhotic Hepatocellular Carcinoma by Yuli Zhang, Dinggui Chen, Miaomiao Yang, Xianfeng Qian, Chunmei Long, Zhongwei Zheng

“…The lncRNA, miRNA, and mRNA expression datasets were obtained from Gene Expression Omnibus and The Cancer Genome Atlas. …”
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Low-Concentration Oxygen/Ozone Treatment Attenuated Radiculitis and Mechanical Allodynia via PDE2A-cAMP/cGMP-NF-κB/p65 Signaling in Chronic Radiculitis Rats by Junnan Wang, Mingyi Wu, Xiaowen Lin, Yun Li, Zhijian Fu

“…Moreover, intrathecal administration of 20 μg/mL oxygen/ozone reversed the increased levels of spinal PDE2A and NF-κB/p65 mRNA and protein expressions in rats with chronic radiculitis. …”
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Morphological and functional analysis of colorectal cancer cell lines in 2D and 3D culture models by Seiichi Shinji, Yutaro Ogawa, Takeshi Yamada, Akihisa Matsuda, Kay Uehara, Yasuyuki Yokoyama, Goro Takahashi, Takuma Iwai, Toshimitsu Miyasaka, Shintaro Kanaka, Koki Hayashi, Yuuki Shichi, Masakazu Fujiwara, Kimimasa Takahashi, Tomio Arai, Toshiyuki Ishiwata, Hiroshi Yoshida

“…In 3D cultures using low-attachment plates, mesenchymal cells from 2D cultures showed reduced vimentin mRNA levels. Morphologically, the five CRAC cell lines appeared similarly shaped in 2D culture but formed different structures in 3D culture. …”
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Identification of the RHD novel allele c. 801+2T>G and study of its effect on RhD phenotype in vitro by JIA Shuangshuang, WEN Jizhi, WEI Ling, ZHANG Runqing, LUO Guangping, JI Yanli

“…By using an in vitro micro gene splicing system, the mRNA splicing results were detected and analyzed using agarose and capillary electrophoresis to predict their impact on RhD phenotype. …”
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Hirudo extract ameliorates proliferative vitreoretinopathy by promoting autophagy and attenuating the THBS2/PI3K/Akt pathway by Hui Huang, Qun Huang, Jing Wang, Wanjie Wang, Yanlin Zheng

“…Autophagy was observed via transmission electron microscopy. The mRNA expression of MMP9, N-cadherin, vimentin, THBS2, PI3K, and Akt was measured via RT‒qPCR. …”
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Resveratrol Alleviates NEFA-Induced Oxidative Damage in Bovine Mammary Epithelial Cells by Restoring Mitochondrial Function by Longwei Sun, Junpeng Huang, Xiangyang Dou, Zhenyu Dong, Yuan Li, Shujing Tan, Ran Yu, Chengmin Li, Weiguo Zhao

“…In addition, in vivo studies in a mouse model of oxidative damage induced by high-fat diet (HFD) demonstrated that RES alleviated oxidative damage (decreased MDA content) and mitochondrial dysfunction (decreased expression of Drp1 and Fis1 and increased levels of Mfn2, Cyt C mRNA and ATP production) in mammary gland tissue. …”
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Genes related to neural tube defects and glioblastoma by Rui Cao, Yurong Liu, Kaixin Wei, Ning Jin, Yuxiang Liang, Ruifang Ao, Weiwei Pan, Xiang Wang, Xiuwei Wang, Li Zhang, Jun Xie

“…Our results indicated that two genes at mRNA and protein levels have been well verified in both NTDs mouse and GBM human samples, namely, Poli and Fgf1. …”
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ET-1通过上调CXCR4表达促进鼻咽癌低转移 细胞株6-10B的迁徙

“…【目的】 探讨内皮素-1(ET-1)上调CXCR4表达促进鼻咽癌低转移细胞株6-10B迁徙的机制【方法】 加入不同浓度的ET-1以及同时加入SDF-1α处理6-10B细胞,趋化实验检测ET-1对6-10B细胞迁徙能力的影响分别采用Real-time PCR以及Western Blot方法检测ET-1上调后CXCR4在基因以及蛋白水平的变化Western Blot方法检测用特异性的ETAR抑制剂或者PI3K/AKT/mTOR或MARK1/ERK1/2抑制剂预处理6-10细胞后,ET-1对CXCR4表达水平的变化;并探讨ET-1处理后,PI3K/AKT/mTOR以及MARK1/ERK1/2信号通路中蛋白磷酸化水平的改变【结果】 趋化实验显示6-10B细胞丧失对SDF-1α的趋化能力,但不同浓度的ET-1刺激后6-10B细胞对SDF-1α的迁徙能力明显增强(P < 0.05),10 nmol/L ET-1的作用最明显Real-time PCR以及Western Blot结果显示,不同浓度的ET-1处理6-10B细胞后,ET-1在基因以及蛋白的水平可以呈剂量依赖性及时间依赖性地上调CXCR4的表达用特异性的内皮素受体A(ETAR)抑制剂或者PI3K/AKT/mTOR或MARK1/ERK1/2通路的抑制剂,能够明显抑制ET-1对CXCR4表达的上调Western blot检测显示ET-1能激活PI3K/AKT/mTOR及MAPK1/ERK1/2信号通路【结论】 ET-1能上调6-10B细胞功能性CXCR4的mRNA和蛋白表达,并能明显增强6-10B细胞对SDF-1α的迁徙能力CXCR4的上调主要是通过ETAR介导,激活PI3K/AKT/mTOR和MAPK1/ERK1/2信号通路…”
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Elucidating the Role of HIF-1&alpha;/YAP Signaling Pathway in Regulating Inflammation in Human Periodontal Stem Cells: An in vitro Study by Zhao HW, Liu ST, Wang XJ, Zhang XM, Ma X

“…RT-qPCR confirmed these trends, showing reduced mRNA levels of IL-1β and TNF-α and increased YAP expression in the LPS + DMOG group (P < 0.0001). …”
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Association Study between Novel CYP26 Polymorphisms and the Risk of Betel Quid-Related Malignant Oral Disorders by Shyh-Jong Wu, Yun-Ju Chen, Tien-Yu Shieh, Chun-Ming Chen, Yen-Yun Wang, Kun-Tsung Lee, Yueh-Ming Lin, Pei-Hsuan Chien, Ping-Ho Chen

“…Additionally, we observed a significant mRNA expression of CY26A1 and CYP26B1 in cancerous tissues compared with adjacent noncancerous tissues. …”
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骨肉瘤细胞外泌体激活肺组织成纤维细胞炎性信号通路 by 谢显彪, 温丽丽, 吕东明, 李洪波, 刘伟海, 邹雨桐, 姚浩, 沈靖南

“…结果143B细胞外泌体中TSG101、Flotillin-1、CD63、CD9表达，Calnexin表达缺失(<italic>P</italic>&lt;0.05)；电镜外泌体呈“茶托”样形状，大小为(141.92±52.85) nm；外泌体经裸鼠尾静脉注射后进入肺组织含量显著高于肝、肾、脾、脑等器官（<italic>P</italic>&lt;0.05）；外泌体与人肺成纤维细胞共培养后炎性因子IL-1β、IL-6及TNF-α mRNA水平明显升高（<italic>P</italic>&lt;0.05），同时p-p65，p-ERK及p-p38均显著上调（<italic>P</italic>&lt;0.05）。…”
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环孢霉素A对大鼠心脏移植模型淋巴细胞分子表达与移植心脏病理学改变时相关系的影响

“…实时定量PCR检测淋巴细胞编码基因CD4?CD8 和酪氨酸激酶P59 mRNA表达水平?心肌组织病理学检查判断排斥反应程度?【结果】 ①心脏移植后24 h内,外周血淋巴细胞基因的转录与表达为一过性降低;②心肌组织CD4+?…”
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Seven Immune-Related Genes’ Prognostic Value and Correlation with Treatment Outcome in Head and Neck Squamous Cell Carcinoma by Rui Mu, Yuehong Shen, Chuanbin Guo, Xinyun Zhang, Hongyu Yang, Huijun Yang

“…Finally, the hub gene’s differential mRNA expression levels were evaluated by means of the Gene Expression Profiling Interactive Analysis (GEPIA), and the Human Protein Atlas (HPA) was utilized for the validation of their translational levels. …”
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Circle-seq reveals that eccDNA may be a key blood biomarker for HBV-associated liver cancer by Xiao K. Ran, Xiao F. Zhao, Zhen W. Wei, Hua Z. Pang, Yan F. Tang, Rong Liu, Tie X. Wu, Xu D. Liu

“…The identified eccDNA is combined with mRNA to screen target genes using bioinformatics analysis. …”
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New insights into the salt-responsive regulation in eelgrass at transcriptional and post-transcriptional levels by Huan Zhao, Huan Zhao, Xu Dong, Xu Dong, Dazuo Yang, Dazuo Yang, Qingchao Ge, Qingchao Ge, Peng Lu, Peng Lu, Chang Liu, Chang Liu

“…Eelgrass (Zostera marina), a representative marine angiosperm, serves as an ideal model for investigating the mechanisms underlying salt tolerance.MethodsThis study integrated mRNA, sRNA, and degradome sequencing data to identify key genes associated with salt tolerance in eelgrass.ResultsThe results indicate that a series of genes involved in biological processes such as “in response to water deprivation” and “biosynthesis of secondary metabolites” respond to salt stress. …”
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间充质干细胞通过诱导M2型巨噬细胞治疗急性肺损伤 by 孙瑶, 吕海金, 易小猛, 郭俊, 易慧敏

“…BALF中巨噬细胞CD206阳性细胞的比例，ALI+MSC组较ALI组明显升高。在CD206和IL-10的mRNA水平上，ALI+MSC组高于ALI组（P<0.05）。BALF中炎症因子TNF-α浓度ALI+MSC组较ALI组明显降低（P<0.05）。…”
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Quercetin inhibits truncated isoform of dopamine- and cAMP-regulated phosphoprotein as adjuvant treatment for trastuzumab therapy resistance in HER2-positive breast cancer by Han-Sheng Chang, Tzu-Chun Cheng, Shih-Hsin Tu, Chih-Hsiung Wu, You-Cheng Liao, Jungshan Chang, Min-Hsiung Pan, Li-Ching Chen, Yuan-Soon Ho

“…To evaluate the t-DARPP expression in BC, paired tumors and surrounding normal tissues were analyzed by real-time polymerase chain reaction and confirmed higher DARPP-32 kDa family mRNA expression in HER2+ BC tumor tissues. We established 2 patient-derived xenografts (PDX) mice models to test the efficacy of trastuzumab, named model 1 (non-responder) and model 2 (responder). t-DARPP and p95-HER2 protein-protein interactions were detected in PDX tumor tissue from non-responders using Förster resonance energy transfer assays. …”
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