Lnc-1708对体外人骨髓间充质干细胞成骨分化的抑制效应 by 曹峻川, 孙翔, 赵建江

“…分别转染lncRNA-1708过表达慢病毒及lncRNA-1708干扰质粒，获得稳定的hBMSC细胞株后成骨诱导分化14d，用qRT-PCR检测Runt相关转录因子2（RUNX2）、碱性磷酸酶（ALP）mRNA的表达并行碱性磷酸酶染色。【结果】相较成骨诱导分化前，3组hBMSC成骨诱导分化7d后lncRNA-1708表达量均降低（P<0.001）。…”
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Paradoxical Glucose-Sensitizing yet Proinflammatory Effects of Acute ASP Administration in Mice by Alexandre Fisette, Pegah Poursharifi, Katerina Oikonomopoulou, Mercedes N. Munkonda, Marc Lapointe, Katherine Cianflone

“…ASP supplementation induced a proinflammatory phenotype, with higher levels of cytokines including IL-6 and TNF-α in plasma and in adipose tissue, liver, and skeletal muscle mRNA. Additionally, ASP increased M1 macrophage content of these tissues. …”
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Vitamin D Regulates the Expression of Glucocorticoid Receptors in Blood of Severe Asthmatic Patients by Bassam Mahboub, Saba Al Heialy, Mahmood Yaseen Hachim, Rakhee K. Ramakrishnan, Ashraf Alzaabi, Rania Medhat Seliem, Laila Ibraheem Salameh, Sameen Masooma Toor, Fathelrahman Salem Shendi, Ola Mohamed Al Ali, Basil Khalid Safarini, Wafa Taleb Erabia, Rabih Halwani, Qutayba Hamid

“…Upon VitD supplementation of severe asthmatic patients, there was a significant increase in the blood levels of GR-α with no change in GR-β mRNA expression. VitD supplementation also suppressed the blood levels of IL-17F and IL-4. …”
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Regulatory Effect of Iguratimod on the Balance of Th Subsets and Inhibition of Inflammatory Cytokines in Patients with Rheumatoid Arthritis by Yunzhi Xu, Qi Zhu, Jinglve Song, Hongli Liu, Yutong Miao, Fan Yang, Feiyan Wang, Wenjing Cheng, Yebin Xi, Xiaoyin Niu, Dongyi He, Guangjie Chen

“…Frequencies of Th1, Th17, Tfh, and Treg were quantified using flow cytometry, and serum cytokines were detected by enzyme-linked immunosorbent assay. mRNA expression of cytokines and transcriptional factor were quantified by RT-PCR. …”
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New insights into the mechanism of triphenyl phosphate and its metabolite diphenyl phosphate in diabetic kidney disease by Ting Fang, Qiaoyan Liu, Xinxin Huangfu, Hongkai Zhu, Hongwen Sun, Liming Chen

“…The study also detected mRNA expression of core targets molecularly docked with TPHP and DPHP using real-time-PCR. …”
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Exacerbation of diabetes due to F. Nucleatum LPS-induced SGLT2 overexpression in the renal proximal tubular epithelial cells by Aiko Seki, Koichiro Kajiwara, Jumpei Teramachi, Masahiko Egusa, Takuya Miyawaki, Yoshihiko Sawa

“…The renal tissue SGLT2 mRNA amounts and the number of renal proximal tubules with overexpressed SGLT2 in the lumen were more in the Fn-LPS-administered-diabetic mice than in diabetic mice. …”
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HLA-DMB correlates with antitumor immunity and an improved prognosis in endometrial carcinoma tumors by Xiaoyu Xi, Xiaona Zhang, Jianxin Tang, Xiumei Fan, Jiexian Du

“…Finally, the expression of HLA-DMB was validated by qPCR and immunohistochemistry.ResultsThe expression of HLA-DMB at both mRNA and protein levels is significantly higher in UCEC tissues compared to normal tissues. …”
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Integration of machine learning and experimental validation to identify the prognostic signature related to diverse programmed cell deaths in breast cancer by Longpeng Li, Longpeng Li, Jinfeng Zhao, Yaxin Wang, Zhibin Zhang, Wanquan Chen, Jirui Wang, Yue Cai

“…Clofibrate and imatinib could serve as potential small-molecule complexes targeting SLC7A5 and BCL2A1, respectively. The mRNA expression levels of seven genes were upregulated in clinical cancer tissues.ConclusionPCDRS can be used as a biomarker to assess the prognosis and treatment response of BRCA patients, which offers novel insights for prognostic monitoring and treatment personalization of BRCA patients.…”
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USP4-mediated CENPF deubiquitylation regulated tumor metastasis in colorectal cancer by Zhongdong Xie, Hanbin Lin, Yuecheng Wu, Yanan Yu, Xintong Liu, Yating Zheng, Xiaojie Wang, Jiashu Wu, Meifang Xu, Yuting Han, Qiongying Zhang, Yu Deng, Lin Lin, Yan Linzhu, Li Qingyun, Xinjian Lin, Ying Huang, Pan Chi

“…Examination of clinical samples confirmed that USP4 expression positively correlates with CENPF protein expression, but not mRNA transcript levels. Taken together, this study describes a novel USP4-CENPF signaling axis which is crucial for CRC metastasis, potentially serving as a therapeutic target and a promising prognostic biomarker for CRC.…”
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Long noncoding RNA Gm4419 promotes mesangial cell proliferation and extracellular matrix accumulation via the miR-455-3p/histone deacetylase 2 axis by Yang Wen, Hua Gan, Qing Zhong, Ying Gong

“…In this study, a potential diabetic nephropathy-related long noncoding-microRNA-mRNA axis, Gm4419-miR-455-3p-Hdac2, was predicted using bioinformatics methods. …”
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RIPK1 expression and inhibition in tauopathies: implications for neuroinflammation and neuroprotection by Ignacio Silva-Llanes, Ignacio Silva-Llanes, Ignacio Silva-Llanes, Enrique Madruga, Enrique Madruga, Ana Martínez, Ana Martínez, Isabel Lastres-Becker, Isabel Lastres-Becker, Isabel Lastres-Becker, Isabel Lastres-Becker

“…Our findings show elevated levels of RIPK1 mRNA levels across various forms of tauopathies, in both mouse models and human tissue samples associated with primary and secondary TAU-related disorders. …”
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Expression and Clinical Significance of DAP3 in Breast Cancer and Effect of Bruceine D on Expression of DAP3:Analysis Based on Data-mining from Bioinformatics by Wenlong WANG, Qingzhi KONG, Hongda LU, Qingdong XIANG, Dianlei LIU

“…In addition, CCLE database analysis indicated that DAP3 was also highly expressed in breast cancer cell lines and its mRNA expression ranked ahead in all kinds of cancer cell lines. …”
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Prognostic significance of PD‐L2 expression in patients with oral squamous cell carcinoma—A comparison to the PD‐L1 expression profile by Manuel Weber, Falk Wehrhan, Christoph Baran, Abbas Agaimy, Maike Büttner‐Herold, Marco Kesting, Jutta Ries

“…Moreover, it should be analyzed if PD‐L2 is differentially expressed in tissue and blood samples of OSCC patients compared to healthy controls and if there is an association of PD‐L2 expression with histomorphologic and prognostic tumor parameters. Methods PD‐L2 mRNA expression was analyzed in tumors and healthy oral mucosa specimens and in corresponding peripheral blood samples of 48 OSCC patients and 26 healthy controls using RT‐qPCR. …”
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REDD1 mediates HDM-induced nuclear-cytoplasmic translocation and release of IL-33 in airway epithelial cells by downregulating Nrf2 by Tian Luo, Wentao Ji, Yuxin Gong, Lichang Chen, Chao Liu, Dandan Zhang, Xi Li, Yanhua Lv

“…To further investigate the role of the REDD1-Nrf2-IL-33 axis in the development of asthma, we employed Nrf2 activators and inhibitors to reassess the impact of REDD1 on IL-33. Results At both mRNA and protein levels, we found that REDD1 was significantly overexpressed in samples from asthma patients (P < 0.05). …”
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Transcriptome datasets of salt-stressed tomato plants treated with zinc oxide nanoparticlesMendeley DataSRR30014447 - SRR30014450SRR30014447 - SRR30014450PRJNA1141123SRR30014447 -... by Mostafa Ahmed, Zoltán Tóth, Diaa Attia Marrez, Roquia Rizk, Donia Abdul-Hamid, Kincső Decsi

“…The datasets were obtained through the utilization of the shallow mRNA sequencing technology. Six datasets from the SRA were uploaded to NCBI. …”
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CircRNA profiling reveals the regulatory role of circPAN3 in Hezuo boars Sertoli cell growth by Haixia Shi, Zunqiang Yan, Hong Du, Bo Zhang, Shuangbao Gun

“…Furthermore, we constructed a circRNA-miRNA-mRNA interaction network and functionally analyzed the target genes. …”
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Local Application of Semaphorin 3A Combined with Adipose-Derived Stem Cell Sheet and Anorganic Bovine Bone Granules Enhances Bone Regeneration in Type 2 Diabetes Mellitus Rats by Xiaoru Xu, Kaixiu Fang, Lifeng Wang, Xiangwei Liu, Yuchao Zhou, Yingliang Song

“…The rat ASC sheets showed stronger osteogenic ability than ASCs in vitro, as indicated by the extracellular matrix mineralization and the expression of osteogenesis-related genes at mRNA level. An ASC sheet combined with Bio-Oss® bone granules promoted bone formation in T2DM rats as indicated by microcomputed tomography (micro-CT) and histological analysis. …”
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Interaction between Angiotensin II and Insulin/IGF-1 Exerted a Synergistic Stimulatory Effect on ERK1/2 Activation in Adrenocortical Carcinoma H295R Cells by An-li Tong, Fen Wang, Yun-ying Cui, Chun-yan Li, Yu-xiu Li

“…Ang II significantly stimulated mRNA expression of CYP11B1 and CYP11B2, and such stimulatory effects were enhanced when cells were cotreated with insulin/IGF-1. …”
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纳米孔长读测序鉴定肝癌细胞中低氧特异性转录本 by 高力, 沈晟齐, 高平

“…结论纳米孔长读长测序技术的应用，不仅成功捕捉到了低氧条件下mRNA的特征性表达模式及特异性转录本，同时为深入探索低氧微环境下的转录组复杂性提供了强有力的技术支撑。…”
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IL-10 and socs3 Are Predictive Biomarkers of Dengue Hemorrhagic Fever by Lilian Karem Flores-Mendoza, Tania Estrada-Jiménez, Virginia Sedeño-Monge, Margarita Moreno, María del Consuelo Manjarrez, Guadalupe González-Ochoa, Lourdes Millán-Pérez Peña, Julio Reyes-Leyva

“…Amounts of interleukins IL-2, IL-4, IL-6, and IL-10, interferon- (IFN-) γ, and tumor necrosis factor- (TNF-) α were quantified by flow cytometry, and the relative expression of socs1 and socs3 mRNA was quantified by real-time RT-PCR. Results. …”
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