Effects of genetic ablation and pharmacological inhibition of HuR on gene expression, iron metabolism, and hormone levels by Nathalie Idlin, Sivakumar Krishnamoorthy, Magdalena Wolczyk, Mouad Fakhri, Michal Lechowski, Natalia Stec, Jacek Milek, Pratik Kumar Mandal, Jaroslaw Cendrowski, Christos Spanos, Magdalena Dziembowska, Katarzyna Mleczko-Sanecka, Juri Rappsilber, Gracjan Michlewski

“…Abstract Background HuR/ELAV1, a ubiquitous RNA-binding protein, belongs to the RNA-binding protein family and is crucial for stabilizing and regulating the translation of various mRNA targets, influencing gene expression. Elevated HuR levels are associated with multiple disorders, including cancer and neurodegenerative diseases. …”
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CD154 Induces Interleukin-6 Secretion by Kidney Tubular Epithelial Cells under Hypoxic Conditions: Inhibition by Chloroquine by Antoine Dewitte, Julien Villeneuve, Sébastien Lepreux, Marion Bouchecareilh, Xavier Gauthereau, Claire Rigothier, Christian Combe, Alexandre Ouattara, Jean Ripoche

“…Both intracellular IL-6 expression and secretion were stimulated by CD154 and associated with a strong upregulation of IL-6 mRNA and increased transcription. Searching for inhibitors of CD154-mediated IL-6 production by HK-2 cells in hypoxic conditions, we observed that chloroquine, a drug that has been repurposed as an anti-inflammatory agent, alleviated this induction. …”
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CircRNAs in colorectal cancer: potential roles, clinical applications, and natural product-based regulation by Jingjing Yang, Jingjing Yang, Qiqi Fan, Yiyun Wang, Yuanyue Liu, Xiaoning Xu, Yeqi Liang, Jiakang Xie, Jiajie Li, Fengting Ai, Yong Cao, Shangzhen Yu, Jinman Liu

“…Additionally, natural products such as flavonoids and glycosides, by modulating circRNA-miRNA-mRNA networks, offer promising therapeutic strategies. …”
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Comparative analysis of galectin-1, galectin-3, and galectin-8 signatures in normal and choriocarcinoma trophoblast cell lines by Jovanović Krivokuća Milica, Legner Janko, Ćujić Danica, Vilotić Aleksandra, Grubiša Ivana, Vićovac Ljiljana, Bojić-Trbojević Žanka

“…Expression levels of these galectins at both mRNA and protein levels were evaluated using PCR, qPCR, and Western blotting, while their cellular localization and distribution were assessed through immunocytochemistry, subcellular fractionation, and Western blotting. …”
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A nociceptor-specific RNAi screen in Drosophila larvae identifies RNA-binding proteins that regulate thermal nociception by Amber Dyson, Gita Gajjar, Katherine C. Hoffman, Dakota Lewis, Sara Palega, Erik Rangel Silva, James Auwn, Andrew Bellemer

“…Other screen isolates have previously described roles in regulating nociceptor morphology and mRNA processing, but less clear roles in regulating nociceptor function. …”
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Clonal Heterogeneity in the Neuronal and Glial Differentiation of Dental Pulp Stem/Progenitor Cells by Fraser I. Young, Vsevolod Telezhkin, Sarah J. Youde, Martin S. Langley, Maria Stack, Paul J. Kemp, Rachel J. Waddington, Alastair J. Sloan, Bing Song

“…Importantly, only DPSC clones with high nestin mRNA expression levels were found to successfully differentiate into Map2 and NF-positive neuronal-like cells. …”
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DNM3OS Enhances the Apoptosis and Senescence of Spermatogonia Associated with Nonobstructive Azoospermia by Providing miR-214-5p and Decreasing E2F2 Expression by Rui Hua, Qingjun Chu, Feiyan Guo, Qinjie Chen, Maocai Li, Xuan Zhou, Yongtong Zhu

“…Microarray assays were performed on samples of testicular biopsy tissue obtained from patients with NOA for the purpose of identifying differentially expressed lncRNAs and messenger RNA (mRNA) transcripts, and the results were verified by quantitative real-time polymerase chain reaction. …”
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Zellweger syndrome; identification of mutations in PEX19 and PEX26 gene in Saudi families by Abdulfatah M. Alayoubi, Ambreen Ijaz, Abdul Wali, Jamil A. Hashmi, Azizah Alharbi, Sulman Basit

“…Moreover, in this study, the importance of a synonymous exonic variant of PEX26 close to the splice donor site was explored in relation to pre-mRNA splicing and resulting disease manifestations.…”
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The effects of Bifidobacterium animalis QC08 on reducing uric acid level and providing renal protection in mice with hyperuricemia by Huijia Mao, Yang Fan, Fang Tan, Xingyao Long

“…Additionally, Bifidobacterium animalis QC08 was found to regulate the mRNA transcription of renal uric acid transporters, leading to significantly upregulation of the expression levels of UAT, ABCG2, and OAT1 genes (p < 0.05).ConclusionBifidobacterium animalis QC08 demonstrates certain uric acid-lowering, anti-inflammatory, and renal protective effects, which are associated with the inhibition of XOD activity and the modulation of the expression levels of uric acid transporter genes (UAT, ABCG2, and OAT1).…”
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CFTR氯通道调控小鼠内毒素血症血小板活化及炎症因子生成 by 胡瑞, 周仪, 李梅, 王冠蕾

“…结果<italic>Cftr</italic>^+/+小鼠静脉注射LPS后，外周血血小板TLR4和P-selectin表达增加、血小板数目减少、血小板表达促炎炎症因子mRNA水平升高，而CFTR全基因敲除加重之（<italic>P</italic>&lt;0.05）。…”
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Investigation of TGFβ1-Induced Long Noncoding RNAs in Endothelial Cells by Krishna K. Singh, Pratiek N. Matkar, Adrian Quan, Laura-Eve Mantella, Hwee Teoh, Mohammed Al-Omran, Subodh Verma

“…This is the first lncRNA and mRNA transcriptome profile of TGFβ-mediated changes in human endothelial cells. …”
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Effectiveness of Voytik-Harbin Protocol in Fabrication of Ram’s Testicular-Derived Hydrogel and Its Impact on Mouse In Vitro Spermatogenesis by Leila Rashki Ghaleno, Tohid Rezaei Topraggaleh, Leila Montazeri, Abdolhossein Shahverdi, Mojtaba Rezazadeh Valojerdi

“…Followingmouse testicular cell culture, TDH and collagen hydrogel did not differ significantly in terms of cell survival ratesand the mRNA expression of early spermatogenesis genes.Conclusion: Using the VH protocol for producing ram TDH resulted in a firm hydrogel with a high frequency ofrepeat, which may be suited for testicular cell growth.…”
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circCOL1A1 Promotes the Progression of Gastric Cancer Cells through Sponging miR-145 to Enhance RABL3 Expression by Yue Ma, Yanyi Ren, Huitao Wen, Chengcheng Cui

“…We performed RT-PCR with divergent primers, mRNA stability assay, and RNase R digestion assay to characterize circCOL1A1 in gastric cancer cell lines. qRT-PCR was applied to detect the level of circCOL1A1 in both gastric cancer cell lines and tissues. …”
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内质网应激参与蛋氨酸-胆碱缺乏饮食诱导的小鼠非酒精性脂肪肝的形成 by 方婉军, 凌文华

“…【结果】MCD 组 NAS 评分和血清中 ALT、AST 以及肝组织中 TG、TC 水平均高于普通膳食组；MCD 组 p- PERK、p-eIF2α、CHOP蛋白表达亦明显高于正常膳食组，而GADD34 mRNA表达显著低于普通膳食组，且随时间推移，MCD 组内质网应激逐渐加重；相关分析表明在MCD组中内质网应激相关指标与NAS评分呈显著相关。…”
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Identification of compounds against atherosclerosis induced by ox-LDL based on cell extraction/UPLC–MS/MS from mulberry twigs and their mechanistic analysis by Zhihui Jin, Wei Xiang, Xiaowen Shi, Fenglin Jiang, Yanan Jia, Yuansong Zhang, Lingshu Zeng, Xianzhi Huang, Li Xu

“…Real-time qPCR and Western blotting were used for mRNA and protein level analysis. Results Two novel active compounds, Kuwanon H and Morusin, and the known Kuwanon G, were identified. …”
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基于生物信息学途径筛选缺血性脑卒中关键基因及药物预测 by 于瑜, 王钟兴

“…最后用Connectivity Map数据库预测脑卒中的潜在作用药物。结果共筛出521个差异mRNA，其中421个上调、100个下调，其中2个关键基因<italic>Drd4</italic>（<italic>P</italic>=0.000 019）和<italic>Hcar2</italic>（<italic>P</italic>=0.000 094）经qRT-PCR实验证实在脑缺血后表达均升高。…”
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The Maternal Microbiome Programs the m6A Epitranscriptome of the Mouse Fetal Brain and Intestine by Zhuoyu Xiao, Sun Liu, Zengguang Li, Jinru Cui, Hailan Wang, Zihan Wang, Qihuan Ren, Laixin Xia, Zhijian Wang, Yuan Li

“…Although it has been shown that the microbiome affects the mRNA m6A modification of the host, it remains unclear whether the maternal microbiome affects m6A epitranscriptome of the fetus so as to impact fetal development. …”
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DDX50 cooperates with STAU1 to effect stabilization of pro-differentiation RNAs by Weili Miao, Douglas F. Porter, Zurab Siprashvili, Ian D. Ferguson, Luca Ducoli, Duy T. Nguyen, Lisa A. Ko, Vanessa Lopez-Pajares, Suhas Srinivasan, Audrey W. Hong, Yen-Yu Yang, Zhongwen Cao, Robin M. Meyers, Jordan M. Meyers, Shiying Tao, Yinsheng Wang, Paul A. Khavari

“…These findings uncover a DDX50-mediated mechanism of reprograming STAU1 from its canonical role in Staufen-mediated mRNA decay to an opposite role stabilizing pro-differentiation RNAs and establish an activity for glucose in controlling RNA structure and stability.…”
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慢病毒介导靶向PRL-3 基因的siRNA 抑制肝癌细胞的侵袭性

“…【目的】 探讨肝再生磷酸酶-3(PRL-3)的表达对肝癌细胞侵袭能力的影响65377;【方法】 设计65380;合成4对针对PRL-3 基因siRNA 的寡核苷酸序列,分别构建质粒,转染293T 细胞,据其对PRL-3 的抑制率,筛选最有效的PRL-3 基因RNAi 靶序列,设计并合成其siRNA 的双链DNA Oligo,与含绿色荧光蛋白(GFP)编码基因的pGCL-GFP 线性化载体连接产生重组慢病毒质粒LV-PRL3-SiRNA,并行PCR 和测序鉴定65377;将LV-PRL3-SiRNA,pHelper 1.0 和pHelper 2.0三种质粒DNA共转染293T细胞,包装产生慢病毒,以293T细胞GFP蛋白的表达水平经孔稀释法测定病毒滴度65377;重组慢病毒感染肝癌SMCC7721 细胞,行real time-PCR和western-blot验证PRL-3基因的沉默效果65377;用Transwell侵袭试验检测SMCC7721细胞体外侵袭力的改变65377;【结果】 成功构建PRL-3 基因RNA干扰慢病毒载体并获得相应的慢病毒,浓缩病毒悬液的滴度为6 × 108 Tu/mL65377;和对照组相比,RNA 干扰组SMMC7721 细胞PRL-3 基因的mRNA 水平和蛋白水平的抑制率分别为75%和63%;其穿过人工基底膜的平均数(14.2 ± 0.8)明显少于空白对照组(33.0 ± 1.6)及非特异性siRNA感染组(31.2 ± 0.8),侵袭抑制率为58% (P < 0.01)65377;【结论】 降低PRL-3 的表达可抑制肝癌细胞的侵袭能力65377;…”
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Phenolic compounds from Chaenomeles speciosa alleviate inflammation in lipopolysaccharide-treated RAW264.7 macrophages via the NF-κB and MAPK pathways by Fuxia Hu, Chao Liu, Fengqin Wang, Changxin Zhou, Maotong Zhu, Dongxiao Sun-Waterhouse, Zhaosheng Wang

“…The extract suppressed the levels of nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β), and the mRNA and protein expressions of nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). …”
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