Effect of opercular deformity on morphological and transcriptomic profiling of gills in golden pompano (Trachinotus ovatus) by Yuwei Wu, Qian Ma, Dihao Huang, Liuyong Wang, Qiling Zhou, Chunli Ran, Gang Chen

“…Paraffin section, Hematoxylin-Eosin (HE) staining, and TdT-mediated dUTP nick-end labeling (TUNEL) of the gills revealed that no significant difference in morphological structure or apoptosis was identified between the gills collected from the deformed and normal sides. …”
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Masked Modeling-Based Ultrasound Image Classification via Self-Supervised Learning by Kele Xu, Kang You, Boqing Zhu, Ming Feng, Dawei Feng, Cheng Yang

“…Notably, our method does not rely on externally labeled data, allowing us to extract representative features without the need for human annotation. …”
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A machine learning based algorithm accurately stages liver disease by quantification of arteries by Zhengxin Li, Xin Sun, Zhimin Zhao, Qiang Yang, Yayun Ren, Xiao Teng, Dean C. S. Tai, Ian R. Wanless, Jörn M. Schattenberg, Chenghai Liu

“…AD was counted using qVessel (previously trained on manually labeled vessels by stained slides (CD34/a-SMA/CK19) and developed by a decision tree algorithm). …”
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A Named Entity Recognition Model for Chinese Electricity Violation Descriptions Based on Word-Character Fusion and Multi-Head Attention Mechanisms by Lingwen Meng, Yulin Wang, Yuanjun Huang, Dingli Ma, Xinshan Zhu, Shumei Zhang

“…Finally, the CRF layer decodes the output to generate the globally optimal label sequence with the highest probability. Experimental results on the constructed power grid field operation violation description dataset demonstrate that the proposed NER model outperforms the traditional BERT-BiLSTM-CRF model, with an average improvement of 1.58% in precision, recall, and F1-score. …”
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Deep imaging of LepR+ stromal cells in optically cleared murine bone hemisections by Yuehan Ni, Jiamiao Wu, Fengqi Liu, Yating Yi, Xiangjiao Meng, Xiang Gao, Luyi Xiao, Weiwei Zhou, Zexi Chen, Peng Chu, Dan Xing, Ye Yuan, Donghui Ding, Ge Shen, Min Yang, Ronjie Wu, Ling Wang, Luiza Martins Nascentes Melo, Sien Lin, Xiaoguang Cheng, Gang Li, Alpaslan Tasdogan, Jessalyn M. Ubellacker, Hu Zhao, Shentong Fang, Bo Shen

“…The bone hemisections are compatible with diverse antibody labeling strategies. Confocal microscopy of these transparent samples allows for qualitative and quantitative image analysis using Aivia or Bitplane Imaris software, assessing a spectrum of parameters. …”
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Increased Depth of Cellular Imaging in the Intact Lung Using Far-Red and Near-Infrared Fluorescent Probes by Abu-Bakr Al-Mehdi, Mita Patel, Abu Haroon, Darla Reed, B. Ohlsson-Wilhelm, K. Muirhead, Brian D. Gray

“…Transpelural imaging of tumor cells labeled with long-wavelength probes and expressing green fluorescent protein (GFP; excitation/emission 488/507 nm) was done in the intact rat lung after perfusate administration or intravenous injection. …”
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Clinical Outcome between Ticagrelor versus Clopidogrel in Patients with Acute Coronary Syndrome and Diabetes by Peixun He, Xiaolin Luo, Jiabei Li, Yi Li, Xiaozeng Wang, Lan Huang, Jun Jin, Yaling Han

“…The study was a single-center, prospective, randomized, open-label, blinded endpoint, and controlled registry trial. …”
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Virtual reality support during systemic cancer therapy to improve anxiety/depressive symptoms and reduce toxicity in patients with gastrointestinal cancers—OncoVR by S. Kasper, S. Liszio, K. Schorrmann, M. Gerigk, S. Jovic, O. Basu, K. Kostbade, B. Goraus, A. Elsakka, B. Puladi, J. Kleesiek, M. Schuler, G. Luijten, J. Egger

“…Patients and methods: OncoVR is a randomized, open-label, cross-over trial to investigate the feasibility and impact of VR support during cancer therapy to improve anxiety, depressive symptoms, and toxicity in patients with gastrointestinal cancers. …”
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3D Semantic VSLAM of Indoor Environment Based on Mask Scoring RCNN by Chongben Tao, Yufeng Jin, Feng Cao, Zufeng Zhang, Chunguang Li, Hanwen Gao

“…In view of existing Visual SLAM (VSLAM) algorithms when constructing semantic map of indoor environment, there are problems with low accuracy and low label classification accuracy when feature points are sparse. …”
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Temporal and spatial pattern of DNA damage in neurons following spinal cord Injury in mice by Elle EM Scheijen, Naomi Veeningen, Sam Duwé, Anna Ivanova, Jana Van Broeckhoven, Sven Hendrix, David M Wilson

“…Results: Our analysis revealed that increased DNA damage foci were present from 1 hpi to 3 dpi in SCI mice relative to controls (sham surgery and naive), with the damage signal spreading over time longitudinally from the affected area to more rostral and caudal regions. Co-labeling of γH2AX with NeuN revealed neuronal specificity of DNA damage, with increased early cell death (pan-nuclear γH2AX) peaking at 1 dpi and apoptosis (cleaved Caspase-3) arising later at 3 dpi. …”
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Enhanced multiscale human brain imaging by semi-supervised digital staining and serial sectioning optical coherence tomography by Shiyi Cheng, Shuaibin Chang, Yunzhe Li, Anna Novoseltseva, Sunni Lin, Yicun Wu, Jiahui Zhu, Ann C. McKee, Douglas L. Rosene, Hui Wang, Irving J. Bigio, David A. Boas, Lei Tian

“…Traditional histology reveals micro- and meso-scale brain features but suffers from staining variability, tissue damage, and distortion, which impedes accurate 3D reconstructions. The emerging label-free serial sectioning optical coherence tomography (S-OCT) technique offers uniform 3D imaging capability across samples but has poor histological interpretability despite its sensitivity to cortical features. …”
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PSCA-targeted BPX-601 CAR T cells with pharmacological activation by rimiducid in metastatic pancreatic and prostate cancer: a phase 1 dose escalation trial by Mark N. Stein, Ecaterina E. Dumbrava, Benjamin A. Teply, Usama S. Gergis, Martin E. Guiterrez, Ran Reshef, Sumit K. Subudhi, Céline F. Jacquemont, Joseph H. Senesac, J. Henri Bayle, Charity D. Scripture, Monica S. Chatwal, Mehmet A. Bilen, Walter M. Stadler, Carlos R. Becerra

“…Abstract Here we report results of a phase 1 multi-institutional, open-label, dose-escalation trial (NCT02744287) of BPX-601, an investigational autologous PSCA-directed GoCAR-T® cell product containing an inducible MyD88/CD40 ON-switch responsive to the activating dimerizer rimiducid, in patients with metastatic pancreatic (mPDAC) or castration-resistant prostate cancer (mCRPC). …”
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A Video-Based Cognitive Emotion Recognition Method Using an Active Learning Algorithm Based on Complexity and Uncertainty by Hongduo Wu, Dong Zhou, Ziyue Guo, Zicheng Song, Yu Li, Xingzheng Wei, Qidi Zhou

“…Then, an active learning algorithm based on complexity and uncertainty was proposed to automatically select the most valuable samples, thereby reducing the cost of sample labeling and model training. Finally, the effectiveness, superiority, and real-time performance of the proposed method were verified utilizing the MMI Facial Expression Database and some real-time-collected data. …”
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Melatonin in Male Dromedary Camel (<i>Camelus dromedarius</i>) Seminal Plasma and Its Specific MT1 and MT2 Receptors on Sperm Membranes by Lamia Doghbri, Melissa Carvajal-Serna, Moufida Atigui, Adriana Casao, Victoria Peña-Delgado, Mabrouk-Mouldi Seddik, Mohamed Dbara, Rosaura Pérez-Pé, Mohamed Hammadi

“…Two melatonin receptors (MT1, MT2) were detected on camel spermatozoa, with several immunotypes labeled mainly in the tail and post-acrosome region, but also in the acrosome and neck. …”
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Deterministic genetic barcoding for multiplexed behavioral and single-cell transcriptomic studies by Jorge Blanco Mendana, Margaret Donovan, Lindsey Gengelbach O'Brien, Benjamin Auch, John Garbe, Daryl M Gohl

“…This method, called Targeted Genetically-Encoded Multiplexing (TaG-EM), involves inserting a DNA barcode just upstream of the polyadenylation site in a Gal4-inducible UAS-GFP construct so that the barcode sequence can be read out during single-cell sequencing, labeling a cell population of interest. By creating many such independently barcoded fly strains, TaG-EM enables positive identification of cell types in cell atlas projects, identification of multiplet droplets, and barcoding of experimental timepoints, conditions, and replicates. …”
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Smart surgical telementoring system with patch-to-pixel point tracking method by Yanwei Qu, Ling Li, Tongtong Li, Jieyu Zheng, Wentao Tang, Xiaojian Li

“…Following this, feature matching is performed on the tracked patch in the current frame and the square patch in the labelled frame or template frame. The affine transformation is then calculated based on the feature matching results. …”
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DNA Damage Sensor γ-H2AX Is Increased in Preneoplastic Lesions of Hepatocellular Carcinoma by Yasunobu Matsuda, Toshifumi Wakai, Masayuki Kubota, Mami Osawa, Masaaki Takamura, Satoshi Yamagiwa, Yutaka Aoyagi, Ayumi Sanpei, Shun Fujimaki

“…In 40 (69.9%) of 58 cases with HCC, the labeling index (LI) of γ-H2AX was above 50% and was inversely correlated with the histological grade. …”
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The first multiple center prospective study of rhFSH CTP in patients undergoing assisted reproductive technology in China by Longmei Wu, Huayan Yin, Lingfang Guan, Guanjian Li, Junqiang Zhang, Qunshan Shen, Xiaoqing Ni, Chao Wang, Tianjuan Wang, Hao Geng, Chuan Xu, Yunxia Cao, Xiaojin He, Bing Song

“…Abstract We assessed the safety and efficacy of rhFSH-CTP, a novel long-acting FSH agent, in controlled ovarian hyperstimulation for patients undergoing ART. A multi-center, open-label, randomized, positive-control, non-inferiority clinical trial was conducted. …”
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Safety and Biodistribution of an Autologous Bone Marrow-Derived Mononuclear Cell Infusion into Renal Arteries in Patients with Focal Segmental Glomerulosclerosis: A Phase 1 Study by Bruno Freire Botelho, André Luis Barreira, Marcio Gomes Filippo, Karina Dutra Asensi, Lanuza A P Faccioli, Anna Beatriz dos Santos Salgado, Elizabeth Figueiredo de Salles, Carlos Eduardo Cruz Marques, Pedro Leme Silva, Regina Coeli dos Santos Goldenberg, Angelo Maiolino, Bianca Gutfilen, Sergio Augusto Lopes de Souza, Maurilo Leite Junior, Marcelo Marcos Morales

“…Five patients with refractory FSGS and an estimated glomerular filtration rate (eGFR) between 20 and 40 ml/min/1.73 m2 underwent bone marrow aspiration and received an arterial infusion of autologous BMDMCs (5 × 107) for each kidney. In addition, BMDMCs labeled with technetium-99m (99mTc-BMDMCs) were used to assess the biodistribution by scintigraphy. …”
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Effects of Stromal Cell-Derived Factor-1α Secreted in Degenerative Intervertebral Disc on Activation and Recruitment of Nucleus Pulposus-Derived Stem Cells by Jinwei Ying, Zhihua Han, Shishen Pei, Linghao Su, Dike Ruan

“…After the systemic delivery of exogenous PKH26-labelled NPSCs into the rats in vivo, there was a significant difference in the distribution of the migrated cells between the normal and the degenerative IVDs, which might be caused by the different expression levels of SDF-1α. …”
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