MCU expression in hippocampal CA2 neurons modulates dendritic mitochondrial morphology and synaptic plasticity by Katy E. Pannoni, Quentin S. Fischer, Renesa Tarannum, Mikel L. Cawley, Mayd M. Alsalman, Nicole Acosta, Chisom Ezigbo, Daniela V. Gil, Logan A. Campbell, Shannon Farris

“…Using a CA2-specific MCU knockout (cKO) mouse, we found that MCU deletion impairs plasticity at distal dendrite synapses. …”
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Transcriptomic Analysis Reveals an Altered Hcy Metabolism in the Stria Vascularis of the Pendred Syndrome Mouse Model by Wenyue Xue, Yuxin Tian, Yuanping Xiong, Feng Liu, Yanmei Feng, Zhengnong Chen, Dongzhen Yu, Shankai Yin

“…The altered expression of Bhmt results in a dramatic change in multiple biochemical reactions and a disruption of nutrient homeostasis in the endolymph which may contribute to hearing loss of Slc26a4 knockout mouse.…”
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Transcriptomic Profile Analysis of Brain Tissue in the Absence of Functional TRPM8 Calcium Channel by Erick B. Saldes, Alexandra Erdmier, Jai Velpula, Timothy E. Koeltzow, Michael X. Zhu, Swapna Asuthkar

“…<b>Methods:</b> RNA was isolated and purified for microarray analysis collected from male wild-type and TRPM8 knockout mice. <b>Results:</b> We identified various differentially expressed genes tied to multiple signaling pathways. …”
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Cytotoxic mechanisms of pemetrexed and HDAC inhibition in non-small cell lung cancer cells involving ribonucleotides in DNA by Tobias Solli Iveland, Lars Hagen, Mirta Mittelstedt Leal de Sousa, Nina Beate Liabakk, Per Arne Aas, Animesh Sharma, Bodil Kavli, Geir Slupphaug

“…Importantly, CRISPR/Cas9 knockout of the uracil-DNA glycosylase UNG did not affect γH2AX activation or sensitivity to pemetrexed. …”
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MYSM1 attenuates osteoarthritis by recruiting PP2A to deubiquitinate and dephosphorylate RIPK2 by Kang Wei, Chuankun Zhou, Zixing Shu, Xingru Shang, Yi Zou, Wei Zhou, Huanhuan Xu, Yulin Liang, Tian Ma, Xuying Sun, Jun Xiao

“…Genetic or adenovirus-induced MYSM1 knockout exacerbated OA progression in mice, whereas MYSM1 overexpression mitigated it. …”
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NRBF2 plays a crucial role in the acquisition process of learning and memory, independent of the Vps34 complex by Songfen Wu, Songfen Wu, Songfen Wu, Haicai Zhuang, Haicai Zhuang, Haicai Zhuang, Xidan Zhou, Kuan Li, Kuan Li, Kuan Li

“…IntroductionNRBF2, a component of autophagy-associated PIK3C3/VPS34-containing phosphatidylinositol 3-kinase complex, plays a crucial role in learning and memory processes, yet its specific impact on memory and the underlying molecular mechanisms remains unclear.MethodsHere, we utilized NRBF2 knockout mice to examine its influence on the time course of fear memory. …”
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A guide to selecting high-performing antibodies for STING1 (Uniprot ID: Q86WV6) for use in western blot, immunoprecipitation, and immunofluorescence [version 2; peer review: 2 appr... by Vera Ruíz Moleón, Carl Laflamme, Kathleen Southern, Riham Ayoubi, Charles Alende, Maryam Fotouhi

“…This study aims to improve reliability of STING1 research as we have characterized sixteen STING1 commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. …”
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Discoidin domain receptor 2 is an important modulator of BMP signaling during heterotopic bone formation by Fashuai Wu, Chunxi Ge, Haichun Pan, Yuanyuan Han, Yuji Mishina, Vesa Kaartinen, Renny T. Franceschi

“…Consistent with this distribution, conditional knockout of Ddr2 in Gli1-expressing cells inhibited bone formation to the same extent seen in globally Ddr2-deficient animals. …”
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Sex differences in the relationship between body composition and MASLD progression in a murine model of metabolic syndrome by Laura Manjarrés, Aline Xavier, Leticia González, Camila Garrido, Flavia C. Zacconi, Katherine Rivera, Laura Parra, Alkystis Phinikaridou, Cecilia Besa, Marcelo E. Andia

“…This study used an endothelial nitric oxide synthase knockout (eNOS KO) murine model to investigate sex-specific MASLD progression under a Western diet intervention. …”
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Fn14 Deficiency Ameliorates Anti-dsDNA IgG-Induced Glomerular Damage in SCID Mice by Jiawen Wu, Xiaoyun Min, Li Wang, Jie Yang, Ping Wang, Xingyin Liu, Yumin Xia

“…Between the wild-type (WT) and knockout (KO) mice injected with anti-dsDNA IgG hybridoma cells, the latter showed a decrease in both proteinuria and glomerular IgG deposition. …”
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A guide to selecting high-performing antibodies for Huntingtin (UniProt ID: P42858) for use in western blot, immunoprecipitation, and immunofluorescence [version 2; peer review: 2... by Rachel J. Harding, Carl Laflamme, Sara González Bolívar, Aled M. Edwards, Kathleen Southern, Rebeka Fanti, Riham Ayoubi, Charles Alende, Maryam Fotouhi, Renu Chandrasekaran

“…Here we have characterized twenty Huntingtin commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. …”
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Arabidopsis Calcium Dependent Protein Kinase 3, and Its Orthologues OsCPK1, OsCPK15, and AcCPK16, Are Involved in Biotic and Abiotic Stresses by Gardette R. Valmonte-Cortes, Colleen M. Higgins, Robin M. MacDiarmid

“…Overexpression mutants in both Arabidopsis and kiwifruit showed slight tolerance to drought, while knockout mutants were slightly more susceptible or had little difference with wild-type plants. …”
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OMP38 of Carbapenem‐Resistant Acinetobacter Baumannii‐Mediated mtDNA Release Activates the cGAS‐STING Signaling to Induce Inflammatory Response by Yang Yang, Yuanyuan Zeng, Jianjie Zhu, Jianjun Li, Lei Gu, Lin Wei, Zeyi Liu, Jian‐an Huang

“…Here, it is reported that CRAB infection induced a cytosolic DNA‐sensing signaling pathway and significant IFN‐β production in mice post‐CRAB infection. The knockout of STING reduced bacterial burden, the production of inflammatory cytokines, and lung injury in mice post CRAB infection. …”
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NPC1 controls TGFBR1 stability in a cholesterol transport-independent manner and promotes hepatocellular carcinoma progression by Shuangyan Li, Lishan Yan, Chaoying Li, Lijuan Lou, Fengjiao Cui, Xiao Yang, Fuchu He, Ying Jiang

“…Here, we demonstrate that knockout of Npc1 in hepatocytes attenuates hepatocellular carcinoma (HCC) progression in both DEN (diethylnitrosamine)-CCl4 induced and MYC-driven HCC mouse models. …”
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pir-hsa-216911 inhibit pyroptosis in hepatocellular carcinoma by suppressing TLR4 initiated GSDMD activation by Zhouxiang Liao, Lichao Yang, Xiaojing Cheng, Xuejing Huang, Qi Zhang, Daoqiang Wen, Zhenyu Song, Yasi Li, Sha Wen, Yongfeng Li, Meizhen Ou, Zhangnan Huang, Tianqi Liu, Min He

“…In the Huh7 cell line, pir-hsa-216911 knockout confirmed its role in suppressing the TLR4/NFκB/NLRP3 pathway by silencing TLR4. …”
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Shionone Inhibits Glomerular Fibirosis by Suppressing NLRP3 Related Inflammasome though SESN2-NRF2/HO-1 Pathway by Tian Xiao, Hanzhen Zhao, Yucong Wang, Mengyin Chen, Cong Wang, Chen Qiao

“…We also preformed experimentation on SESN2 deficiency models (SESN2 knockout mice and SESN2 siRNA in cells) to further prove our hypothesis. …”
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Smad4 deficiency in hepatocytes attenuates NAFLD progression via inhibition of lipogenesis and macrophage polarization by Wei Yang, Xuanxuan Yan, Rui Chen, Xin Xin, Shuang Ge, Yongxiang Zhao, Xinlong Yan, Jinhua Zhang

“…Hepatocyte-specific knockout Smad4 mice (AlbSmad4−/−) were first constructed using the Cre-Loxp recombinant system to establish a high-fat diet induced NAFLD model. …”
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Multivalent S2 subunit vaccines provide broad protection against Clade 1 sarbecoviruses in female mice by Peter J. Halfmann, Raj S. Patel, Kathryn Loeffler, Atsuhiro Yasuhara, Lee-Ann Van De Velde, Jie E. Yang, Jordan Chervin, Chloe Troxell, Min Huang, Naiying Zheng, Elizabeth R. Wright, Paul G. Thomas, Patrick C. Wilson, Yoshihiro Kawaoka, Ravi S. Kane

“…Challenge studies in female Fc-γ receptor knockout mice reveal that antibody-based cellular effector mechanisms play a role in protection elicited by these vaccines. …”
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Macrophage Infiltration and ITGB2 Expression in ESCC: A Novel Correlation by Tao Huang, Longqian Wei, Huafu Zhou, Jun Liu

“…Based on our findings and prior studies, we propose a novel hypothesis: inducing M1 macrophages in vitro, knocking out ITGB2, and then reinfusing these ITGB2‐knockout M1 macrophages into ESCC patients may represent a promising new immunotherapy strategy, providing a new avenue for ESCC immunotherapy.…”
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Deficiency in Galectin-3 Promotes Hepatic Injury in CDAA Diet-Induced Nonalcoholic Fatty Liver Disease by Kazuhiro Nomoto, Takeshi Nishida, Yuko Nakanishi, Makoto Fujimoto, Ichiro Takasaki, Yoshiaki Tabuchi, Koichi Tsuneyama

“…We investigated whether galectin-3 is involved in the development of NASH by comparing galectin-3 knockout (gal3−/−) mice and wild-type (gal3+/+) mice with choline-deficient L-amino-acid-defined (CDAA) diet-induced NAFLD/NASH. …”
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