Functionally-informed fine-mapping identifies genetic variants linking increased CHD1L expression and HIV restriction in monocytes by Riley H. Tough, Paul J. McLaren, The International Collaboration for the Genomics of HIV

“…A recent genome-wide association study in individuals of African ancestries identified a region on chromosome 1 significantly associated with decreased HIV set-point viral load. Knockout of the closest gene, CHD1L, enhanced HIV replication in vitro in myeloid cells. …”
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SIRT5 Contributes to Colorectal Cancer Growth by Regulating T Cell Activity by Ke Wang, Zuojian Hu, Cuiping Zhang, Lujie Yang, Li Feng, Pengyuan Yang, Hongxiu Yu

“…In this work, we found that Sirt5 knockout mice were resistant to AOM and DSS-induced colitis-associated colorectal tumorigenesis and the level of IFN-γ in their tumor microenvironment was higher. …”
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The Role of Adrenomedullin in the Renal NADPH Oxidase and (Pro)renin in Diabetic Mice by Michio Hayashi, Akihiro Tojo, Tatsuo Shimosawa, Toshiro Fujita

“…To clarify the role of adrenomedullin in diabetic nephropathy, we investigated the NADPH oxidase expression, renin-secreting granular cell (GC) hyperplasia, and glomerular matrix expansion in the streptozotocin (STZ)-induced diabetic adrenomedullin gene knockout (AMKO) mice compared with the STZ-diabetic wild mice at 10 weeks. …”
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Blocking REDD1/TXNIP Complex Ameliorates HG-Induced Renal Tubular Epithelial Cell Apoptosis and EMT through Repressing Oxidative Stress by Lin Mu, Nan Chen, Yakun Chen, Zhifen Yang, Huandi Zhou, Shan Song, Yonghong Shi

“…By generating the DN model in REDD1 knockout mice, we demonstrated that REDD1 deficiency significantly improved apoptosis and EMT in diabetic mice. …”
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Alpha-actinin-4-mediated FSGS: an inherited kidney disease caused by an aggregated and rapidly degraded cytoskeletal protein. by June Yao, Tu Cam Le, Claudine H Kos, Joel M Henderson, Phillip G Allen, Bradley M Denker, Martin R Pollak

“…Comparison of the phenotype in wild-type, heterozygous, and homozygous Actn4 "knockin" and "knockout" mice, together with our in vitro data, suggests that the phenotypes in mice and humans involve both gain-of-function and loss-of-function mechanisms.…”
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lncRNA ZFAS1 Promotes HMGCR mRNA Stabilization via Binding U2AF2 to Modulate Pancreatic Carcinoma Lipometabolism by Luoluo Wang, Yi Ruan, Xiang Wu, Xinhua Zhou

“…ZFAS1 was kept at a high level in PC tissues versus the normal counterparts. ZFAS1 gene knockout remarkably suppressed PC cell multiplication and invasiveness and decreased the contents of free fatty acids, total cholesterol, triglycerides, and phospholipids. …”
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Using insecticidal compounds to elucidate the potential role of neurotransmitters in Lepidoptera pupal ecdysis by Niranjana Krishnan, Cassandra Gorman, Jillian Stewart, Steven Bradbury, Russell Jurenka

“…An analysis of ecdysis movements showed that neonicotinoid-treated lepidopteran larvae exhibited similar disruptions as observed in CCAP neuron-knockout Drosophila larvae. Based on findings to date, we hypothesize that acetylcholine regulates lepidopteran pupal ecdysis directly through CCAP neurons or by activating their upstream efferent inhibitory (likely GABA-releasing) neurons. …”
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Global Autorecognition and Activation of Complement by Mannan-Binding Lectin in a Mouse Model of Type 1 Diabetes by Esben Axelgaard, Jakob Appel Østergaard, Saranda Haxha, Steffen Thiel, Troels Krarup Hansen

“…To test this hypothesis, we induced diabetes by injection of low-dose streptozotocin in MBL double-knockout (MBL/DKO) mice. Development of diabetes was followed by measurements of blood glucose and urine albumin-to-creatinine ratio. …”
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The BnaBPs gene regulates flowering time and leaf angle in Brassica napus by Jiang Yu, Yi‐Xuan Xue, Rehman Sarwar, Shi‐Hao Wei, Rui Geng, Yan‐Feng Zhang, Jian‐Xin Mu, Xiao‐Li Tan

“…The protein sequence analysis showed two proteins containing conserved domains KNOX I, KNOX II, ELK, and HOX of the KONX protein family. The CRISPR/Cas9 knockout lines exhibited early budding and flowering time, coupled with floral organ abscission earlier and a larger leaf angle. …”
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KYNU as a Biomarker of Tumor-Associated Macrophages and Correlates with Immunosuppressive Microenvironment and Poor Prognosis in Gastric Cancer by Kaiyu Shen, Binyu Chen, Liu Yang, Wencang Gao

“…KYNU was overexpressed in GC cells in vitro, and KYNU knockout significantly inhibited GC cell proliferation and invasion. …”
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Alternative Splicing of Cdh23 Exon 68 Is Regulated by RBM24, RBM38, and PTBP1 by Nana Li, Haibo Du, Rui Ren, Yanfei Wang, Zhigang Xu

“…The splicing of Cdh23 exon 68 is affected in Rbm24 knockdown or knockout cells. Moreover, we also found that PTBP1 inhibits the inclusion of Cdh23 exon 68. …”
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The role of the NOTCH1 signaling pathway in the maintenance of mesenchymal stem cell stemness and chondrocyte differentiation and its potential in the treatment of osteoarthritis by Fuming Wu, Haiquan Yu

“…Results Experimental results demonstrated that NOTCH1 expression can influence the maintenance of MSC stemness and chondrocyte differentiation by regulating EZH2. Knockout of NOTCH1 decreased the expression of chondrocyte markers, while overexpression increased their expression. …”
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Angiotensin-Converting Enzyme 2 Deficiency Aggravates Glucose Intolerance via Impairment of Islet Microvascular Density in Mice with High-Fat Diet by Li Yuan, Ying Wang, Chunli Lu, Xiaoya Li

“…Male wildtype (WT) and ACE2 knockout (ACE2 KO) mice were divided into chow diet group and long-term high-fat diet (HFD) group. …”
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<i>TFL1</i>-like genes in <i>Vigna unguiculata</i> (L.) Walp. with different growth habit types by E. A. Krylova, E. K. Khlestkina

“…The next step towards understanding the role of TFL1-like genes requires obtaining knockout lines based on these genes and studying their phenotype. …”
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Elegant and Innovative Recoding Strategies for Advancing Vaccine Development by François Meurens, Fanny Renois, Uladzimir Karniychuk

“…One challenge is balancing attenuation with yield; however, innovations like Zinc-finger antiviral protein (ZAP) knockout cell lines can enhance vaccine production. …”
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Mechanisms of KRAS inhibitor resistance in KRAS-mutant colorectal cancer harboring Her2 amplification and aberrant KRAS localization by Kohei Maruyama, Yuki Shimizu, Yumi Nomura, Tomoko Oh-hara, Yuki Takahashi, Satoshi Nagayama, Naoya Fujita, Ryohei Katayama

“…In the latter group, PDCs with PIK3CA major mutation showed high sensitivity to PI3K+mTOR co-inhibition, and a PDC with Her2 amplification with PIK3CA minor mutation showed PI3K-AKT pathway dependency but lost KRAS-MAPK dependency by cytoplasmic localization of KRAS. In the PDC, Her2 knockout restored KRAS plasma membrane localization and KRAS inhibitor sensitivity. …”
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CXCL1-Triggered Interaction of LFA1 and ICAM1 Control Glucose-Induced Leukocyte Recruitment during Inflammation In Vivo by Kirsten Buschmann, Lutz Koch, Natascha Braach, Hanna Mueller, David Frommhold, Johannes Poeschl, Peter Ruef

“…Flow chamber experiments, expression analysis, functional depletion, and knockout of key adhesion molecules gave mechanistic insight in involved pathways. …”
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Fluoroquinolone-specific resistance trajectories in E. coli and their dependence on the SOS-response by Lisa Teichmann, Sam Luitwieler, Johan Bengtsson-Palme, Benno ter Kuile

“…Results We exposed Escherichia coli to four fluoroquinolones—ciprofloxacin, enrofloxacin, levofloxacin, and moxifloxacin. Using a recA knockout mutant, deficient in the SOS response, as a control, we assessed how the presence or absence of this pathway affects resistance development. …”
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Transcriptional repression of GTL1 under water‐deficit stress promotes anthocyanin biosynthesis to enhance drought tolerance by Noel Anthony Mano, Mearaj A. Shaikh, Joshua R. Widhalm, Chan Yul Yoo, Michael V. Mickelbart

“…To test the hypothesis, gene expression was profiled by RNA‐seq analysis in emerging and expanding leaves of wild‐type and a drought‐tolerant gtl1‐4 knockout mutant under well‐watered and water‐deficit conditions. …”
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PfFBXO1 is essential for inner membrane complex formation in Plasmodium falciparum during both asexual and transmission stages by Sreelakshmi K. Sreenivasamurthy, Carlos Gustavo Baptista, Christopher M. West, Ira J. Blader, Jeffrey D. Dvorin

“…Following inducible knockout of PfFBXO1, parasites undergo abnormal segmentation and karyokinesis, generating inviable daughters. …”
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