Microcephaly protein ANKLE2 promotes Zika virus replication by Adam T. Fishburn, Cole J. Florio, Thomas N. Klaessens, Brian Prince, Neil A. B. Adia, Nicholas J. Lopez, Nitin Sai Beesabathuni, Sydney S. Becker, Liubov Cherkashchenko, Sophia T. Haggard Arcé, Vivian Hoang, Traci N. Shiu, R. Blake Richardson, Matthew J. Evans, Claudia Rückert, Priya S. Shah

“…We observe that ANKLE2 localization is drastically shifted to sites of NS4A accumulation during infection and that knockout of ANKLE2 reduces ZIKV replication in multiple human cell lines. …”
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The generation and evaluation of TKO/hCD55/hTM/hEPCR gene-modified pigs for clinical organ xenotransplantation by Guoli Huai, Yong Wang, Jiaxiang Du, Zhenhui Cheng, Yuxuan Xie, Jia Zhou, Hongmei Tang, Yanyan Jiang, Xiangyang Xing, Shaoping Deng, Dengke Pan, Dengke Pan, Dengke Pan

“…Blood from gene-edited pigs was used for pathophysiological analysis.ResultsComplete knockout of GGTA1, CMAH, and B4GALNT2 was confirmed in 6GE pigs. …”
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RNA‐Binding Protein Hnrnpa1 Triggers Daughter Cardiomyocyte Formation by Promoting Cardiomyocyte Dedifferentiation and Cell Cycle Activity in a Post‐Transcriptional Manner by Chuling Li, Yijin Chen, Qiqi Chen, Haoxiang Huang, Michael Hesse, Yilin Zhou, Ming Jin, Yu Liu, Yifei Ruan, Xiang He, Guoquan Wei, Hao Zheng, Senlin Huang, Guojun Chen, Wangjun Liao, Yulin Liao, Yanmei Chen, Jianping Bin

“…In contrast, CRISPR/Cas9 technology is used to generate CM‐specific Hnrnpa1 knockout mice. Hnrnpa1 knockout inhibited cardiac regeneration and worsened cardiac function in the neonatal MI model. …”
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Exocyst complex component 1 (Exoc1) loss in dormant oocyte disrupts c-KIT and growth differentiation factor (GDF9) subcellular localization and causes female infertility in mice by Chi Lieu Kim Nguyen, Yumeno Kuba, Hoai Thu Le, Hossam Hassan Shawki, Natsuki Mikami, Madoka Aoki, Nanako Yasuhara, Hayate Suzuki, Saori Mizuno-Iijima, Shinya Ayabe, Yuki Osawa, Tomoyuki Fujiyama, Tra Thi Huong Dinh, Miyuki Ishida, Yoko Daitoku, Yoko Tanimoto, Kazuya Murata, Woojin Kang, Masatsugu Ema, Yuji Hirao, Atsuo Ogura, Satoru Takahashi, Fumihiro Sugiyama, Seiya Mizuno

“…Previous studies have shown that Gdf9 knockout mice impaired proliferation and granulosa cell multilayering in growing follicles. …”
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KEAP1 mutations as key crucial prognostic biomarkers for resistance to KRAS-G12C inhibitors by Linyan Tian, Chengming Liu, Sufei Zheng, Huiyang Shi, Fang Wei, Wenxin Jiang, Yucheng Dong, Haiyan Xu, Enzhi Yin, Nan Sun, Jie He, Yan Wang

“…Using CRISPR/Cas9 technology, this study generated cells with KEAP1 and STK11 knockouts, and utilized lentiviral vectors to overexpress PD-L1. …”
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TXNIP deficiency attenuates renal fibrosis by modulating mTORC1/TFEB-mediated autophagy in diabetic kidney disease by Yunxia Du, Ming Wu, Shan Song, Yawei Bian, Yonghong Shi

“…In this study, we found that TXNIP knockout suppressed renal fibrosis and activation of mammalian target of rapamycin complex 1 (mTORC1) and restored transcription factor EB (TFEB) and autophagy activation in diabetic kidneys. …”
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Nucleotide-Binding Oligomerization Domain-1 and -2 Play No Role in Controlling Brucella abortus Infection in Mice by Fernanda S. Oliveira, Natalia B. Carvalho, Dario S. Zamboni, Sergio C. Oliveira

“…However, NOD1, NOD2, and Rip2 knockout mice were no more susceptible to infection with virulent B. abortus than wild-type mice. …”
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Santa-maria is a glial phagocytic receptor that acts with SIMU to recognize and engulf apoptotic neurons by Reut Hilu-Dadia, Aseel Ghanem, Shelly Vogelesang, Malak Ayoub, Ketty Hakim-Mishnaevski, Estee Kurant

“…Moreover, we reveal that triple knockout of genes for all three glial phagocytic receptors (i.e., simu, draper, and santa-maria) causes partial lethality, thus illuminating their role in development, particularly in the developing nervous system.…”
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Application of Proteomics to Inflammatory Bowel Disease Research: Current Status and Future Perspectives by Arash Assadsangabi, Caroline A. Evans, Bernard M. Corfe, Alan Lobo

“…Genome-wide association studies (GWAS) and knockout mouse models have significantly advanced our understanding of genetic susceptibility loci and inflammatory pathways involved in IBD pathogenesis. …”
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Molecular Imaging of Cathepsin B Proteolytic Enzyme Activity Reflects the Inflammatory Component of Atherosclerotic Pathology and Can Quantitatively Demonstrate the Antiatheroscler... by Dong-Eog Kim, Jeong-Yeon Kim, Dawid Schellingerhout, Soo-Min Shon, Sang-Wuk Jeong, Eo-Jin Kim, Woo Kyung Kim

“…We used a CatB-activatable near-infrared fluorescence (NIRF) imaging agent to demonstrate the inflammatory component in mice atheromata and the atherosclerosis-modulating effects of atorvastatin or glucosamine treatments. Apolipoprotein E knockout mice ( n = 35) were fed normal chow, a Western diet, a Western diet + atorvastatin, a Western diet + glucosamine, or a Western diet + atorvastatin + glucosamine for 14 weeks. …”
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Molecular Circuits of Resolution in the Eye by Elvira L. Liclican, Karsten Gronert

“…Protective endogenous roles of LXA4 and NPD1 have been established utilizing lipidomic analysis, knockout mice, and pharmacological, genetic, and dietary manipulation, providing compelling evidence that these intrinsic lipid autacoid circuits play essential roles in restraining inflammation, promoting wound healing, inhibiting pathological angiogenesis, and providing neuroprotection in the delicate visual axis.…”
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Type I Diabetes-Associated Tolerogenic Properties of Interleukin-2 by Aziz Alami Chentoufi, Simon Gaudreau, Alex Nguyen, Mahmoud Sabha, Abdelaziz Amrani, Geyhad ElGhazali

“…The breakdown of tolerance has been associated with a defect in the number and the function of naturally occurring regulatory T cells (nTreg) that are the master player in peripheral tolerance. Gene knockout experiments in mouse models have shown a nonredundant activity of IL-2 related to its critical role in inducing nTreg and controlling peripheral T cell tolerance. …”
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ECM29/proteasome-mediated self-antigen generation by CNS-resident neuroglia promotes regulatory T cell activation by Sheng-Wen Chen, Ying Chu, Chien-Hsin Chu, Xuan-Dieu Thi Pham, Hang Pong Ng, Chin-Lin Guo, Pei-Lin Cheng

“…Inducible oligodendroglia- or microglia-conditional Ecm29 knockout mice exhibit higher susceptibility to experimental autoimmune encephalomyelitis (EAE) than control counterparts do, coincident with reduced Treg cell populations in the spinal cord. …”
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NLRP7 maintains the genomic stability during early human embryogenesis via mediating alternative splicing by Zhongliang Chen, Liangxia Jiang, Min Su, Qibing Zeng, Peng Luo, Liangzhao Chu

“…Here, we show that NLRP7 knockout affects the genetic stability, resulting in increased DNA damage in both human embryonic stem cells and blastoids, making embryonic cells in blastoids more susceptible to apoptosis. …”
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D-tryptophan triggered epithelial-mesenchymal transition by activating TGF-β signaling pathway by Chong Wang, Fangting Wang, Yanbo Wang, Linglin Fu

“…Moreover, D-tryptophan promoted the expression of transforming growth factor-β (TGF-β) 1, and Smad4 knockout damages D-tryptophan's ability. These results indicated that D-tryptophan stimulated HaCaT cells to produce TGF-β1 and thus activated the TGF-β/Samd pathway, resulting in the triggering of EMT. …”
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F4/80+ Host Macrophages Are a Barrier to Murine Embryonic Stem Cell-Derived Hematopoietic Progenitor Engraftment In Vivo by Heather L. Thompson, Nico van Rooijen, Bryce T. McLelland, Jennifer O. Manilay

“…Murine embryonic stem cell-derived hematopoietic progenitors (ESHPs) were generated via coculture with the bone marrow stromal cell line, OP9, and then transplanted into NOD.SCID.Common Gamma Chain (NSG) knockout mice, which lack B, T, and natural killer cells. …”
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Advances in Murine Models of Diabetic Nephropathy by Li-li Kong, Hao Wu, Wen-peng Cui, Wen-hua Zhou, Ping Luo, Jing Sun, Hang Yuan, Li-ning Miao

“…Although a large number of murine models of DN including artificially induced, spontaneous, and genetically engineered (knockout and transgenic) animal models have been developed, none of them develops renal changes sufficiently reflecting those seen in humans. …”
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TEX10 Promotes the Tumorigenesis and Radiotherapy Resistance of Urinary Bladder Carcinoma by Stabilizing XRCC6 by Sheng Luo, Wenjin Wang, Jingfang Feng, Rui Li

“…Moreover, TEX10 gene knockout reduced the radiotherapy resistance of urinary bladder carcinoma. …”
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Cyclin-Dependent Kinase-Like 5 (CDKL5): Possible Cellular Signalling Targets and Involvement in CDKL5 Deficiency Disorder by Syouichi Katayama, Noriyuki Sueyoshi, Tetsuya Inazu, Isamu Kameshita

“…We also discuss the relationship between changes in the phosphorylation signalling pathways and the Cdkl5 knockout mouse phenotype and consider future prospects for the treatment of mental and neurological disease associated with CDKL5 mutations.…”
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Obesity-induced activation of NADPH oxidase 2 prolongs cardiac repolarization via inhibiting K+ currents. by Bin Li, Yating Chen, Maoxiang Zhao, Zhijie Chen, Zhuhui Lin, Jie Liu, Xueping Wang, Jiancheng Zhang, Yang Li

“…We investigated the effect of Nox2 on cardiac repolarization without compromising its expression and function in other systems using mice with conditional cardiac-specific deletions of Nox2 (knockout [KO]). Wild-type, KO, and Flox littermate mice were randomized to either the control or high-fat diet (HFD) groups. …”
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