Screening semen samples for Zika virus infection: role for serologic and RT-PCR testing by Humberto Ferraz, Humberto Ferraz, Humberto Ferraz, Kevan Akrami, Kevan Akrami, Kevan Akrami, Laise de Moraes, Laise de Moraes, Lais Cambuí Gusmão, Genevieve Coelho, Daniele Freitas, Daniele Freitas, Manoel Barral-Netto, Aldina Barral, Viviane Boaventura, Viviane Boaventura, Luciane Amorim Santos, Luciane Amorim Santos, Luciane Amorim Santos, Ricardo Khouri, Ricardo Khouri

“…Human reproductive clinics typically perform serologic tests to screen for Zika infection.MethodsIn this study, we evaluated semen samples stored in a human reproduction clinic in Salvador, Brazil, during the Zika virus outbreak, using real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) technique.Results36 sperm samples from 23 donors were analyzed, including 5 with positive IgG serology for ZIKV and all negative for IgM. …”
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Speciation and historical invasions of the Asian black-spined toad (Duttaphrynus melanostictus) by Christophe Dufresnes, Daniel Jablonski, Johanna Ambu, Vishal Kumar Prasad, Kumudani Bala Gautam, Rachunliu G. Kamei, Stephen Mahony, Sylvia Hofmann, Rafaqat Masroor, Bérénice Alard, Angelica Crottini, Devin Edmonds, Annemarie Ohler, Jianping Jiang, Janak R. Khatiwada, Sandeep Kumar Gupta, Amaël Borzée, Leo J. Borkin, Dmitriy V. Skorinov, Daniel A. Melnikov, Konstantin D. Milto, Evgeny L. Konstantinov, Sven Künzel, Tomasz Suchan, Dmitriy V. Arkhipov, Alexei V. Trofimets, Tan Van Nguyen, Chatmongkon Suwannapoom, Spartak N. Litvinchuk, Nikolay A. Poyarkov

“…Combining continental-wide genomic and DNA-barcoding analyses, we reconstructed the historical biogeography of the Asian black-spined toad (Duttaphrynus melanostictus), a toxic commensal amphibian that currently threatens two biodiversity hotspots through biological invasions (Wallacea and Madagascar). …”
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Coupling of an Au@AgPt nanozyme array with an micrococcal nuclease-specific responsiveness strategy for colorimetric/SERS sensing of Staphylococcus aureus in patients with sepsis by Xueqin Huang, Yingqi Yang, Hanlin Zhou, Liping Hu, Annan Yang, Hua Jin, Biying Zheng, Jiang Pi, Jun Xu, Pinghua Sun, Huai-Hong Cai, Xujing Liang, Bin Pan, Junxia Zheng, Haibo Zhou

“…Rapid and ultrasensitive detection of pathogen-associated biomarkers is vital for the early diagnosis and therapy of bacterial infections. Herein, we developed a close-packed and ordered Au@AgPt array coupled with a cascade triggering strategy for surface-enhanced Raman scattering (SERS) and colorimetric identification of the Staphylococcus aureus biomarker micrococcal nuclease (MNase) in serum samples. …”
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Electrically reconfigurable surface acoustic wave phase shifters based on ZnO TFTs on LiNbO3 substrate by Yi Zhang, Zilong Xiong, Lewei He, Yang Jiang, Chenkai Deng, Fangzhou Du, Kangyao Wen, Chuying Tang, Qiaoyu Hu, Mujun Li, Xiaohui Wang, Wenhui Wang, Han Wang, Qing Wang, Hongyu Yu, Zhongrui Wang

“…However, current acoustoelectric devices suffer from limited tunability, intricate heterogeneous structures, and complex manufacturing processes, all of which impede their practical applications. In this study, we present a novel material system for voltage-tunable SAW phase shifters. …”
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NaHS alleviates neuropathic pain in mice by inhibiting IL-17-mediated dopamine (DA) neuron necroptosis in the VTA by Jun Wang, Nan Zhang, Hong-Zheng Liu, Jin-Liang Wang, Yong-Bo Zhang, Dong-Dong Su, Li-Min Zhang, Bao-Dong Li, Hui-Tao Miao, Jun Miao

“…This study aimed to investigate the link between IL-17-induced necroptosis of dopamine neurons in the VTA and neuropathic pain. Additionally, we explored whether H2S treatment could reduce the loss of VTA dopamine neurons, thereby lowering neuropathic pain in a chronic constriction injury (CCI) model. …”
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Efficacy and safety of gut microbiome-targeted treatment in patients with depression: a systematic review and meta-analysis by Bo Pan, Yiming Pan, Yu-Song Huang, Meng Yi, Yuwei Hu, Xiaoyu Lian, Hui-Zhong Shi, Mingwei Wang, Guifen Xiang, Wen-Yi Yang, Zhong Liu, Fangfang Xia

“…Abstract Background The study aimed to comprehensively analyze and establish a framework for evaluating the efficacy of microbiome–targeted treatment (MTT) for depression. Methods We searched PubMed, Embase, Cochrane Library, Web of Science, and the Chinese National Knowledge Infrastructure database for randomized controlled trials (RCTs) on MTT in treating depression until October 19, 2023. …”
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Lethal clinical outcome and chemotherapy and immunotherapy resistance in patients with urothelial carcinoma with MDM2 amplification or overexpression by Weijuan Zhang, Jiejie Xu, Hailong Liu, Yu Zhu, Zewei Wang, Han Zeng, Zhaopei Liu, Yuan Chang, Le Xu, Kaifeng Jin, Jingtong Xu, Xiaohe Su, Yawei Ding, Lingkai Zhang, Jiaxing Sun, Yuzhen Wu

“…However, the clinical significance and impact on tumor immune contexture of MDM2 amplification in UC remain unclear.Methods This study analyzed 240 patients with UC with matched clinical annotations from two local cohorts (ZSHS cohort and FUSCC cohort). We assessed the correlation between MDM2 status and clinical outcomes, therapeutic efficacy, and immunological characteristics by immunohistochemical analysis and targeted sequencing. …”
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Effect of Hemoadsorption for Cytokine Removal in Pneumococcal and Meningococcal Sepsis by Francesca Leonardis, Viviana De Angelis, Francesca Frisardi, Chiara Pietrafitta, Ivano Riva, Tino Martino Valetti, Valentina Broletti, Gianmariano Marchesi, Lorenza Menato, Roberto Nani, Franco Marson, Mirca Fabbris, Luca Cabrini, Sergio Colombo, Alberto Zangrillo, Carlo Coniglio, Giovanni Gordini, Lucia Stalteri, Giovanni Giuliani, Vittorio Dalmastri, Gaetano La Manna

“…Available adjunctive treatments to control the inflammatory response have shown encouraging results in the evolution of patients with sepsis and systemic inflammation, but meningococcal or pneumococcal infection has not been investigated. We herein report five patients with similar critical pathological conditions, characterized by pneumococcal or meningococcal sepsis and treated with hemoadsorption for cytokine removal. …”
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Quantitative N-glycoproteomic analysis reveals glycosylation signatures of plasma immunoglobulin G in systemic sclerosis by Lu Cheng, Lu Cheng, Yanhong Li, Yu Zhou, Yingying Ling, Tong Wu, Zongan Liang, Yinlan Wu, Chunyu Tan, Yi Liu, Yong Zhang

“…Nonetheless, the intricate details of intact glycopeptides in SSc remain elusive owing to challenges in their detection. In this study, we characterized plasma immunoglobulin G (IgG) intact N-glycopeptides from 30 SSc patients and 30 healthy controls (HCs) via our recently developed intact glycopeptide analysis method GlycoQuant. …”
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Energy and Macronutrients Intakes among Childbearing Age Women Living in the Urban Area of Morocco: A Cross-Sectional Study by Fatima Barich, Fatima Zahra Laamiri, Slimane Mehdad, Souad Benaich, Anass Rami, Mohamed Idrissi, Chaimae Serbouti, Houria Lahmame, Hasnae Benkirane, Manelle Rjimati, Amina Barkat, El Arbi Rjimati, Hassan Aguenaou

“…In Morocco, these changes have led to serious nutritional disorders and increased risk of morbidity and mortality particularly among vulnerable groups such as women of childbearing age. We aimed to assess the average daily energy and macronutrient intakes and to investigate their association with socioeconomic factors and weight status among women aged 19–49 years in urban areas. …”
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Genotypic difference in response to copper stress in upland cotton as revealed by physiological and molecular expression analyses by Jianfei Wu, Tao Wang, Yin Huang, Shuiping Xiao, Xiaoxia Luo, Yanfeng Deng, Xiu Yang, Qingquan Kong, Feiyu Tang

“…However, the Cu toxicity tolerance mechanism in cotton remains largely obscure. To address the issue, we first identified two cotton lines contrasting in response to Cu toxicity by examining 12 morphological and physiological attributes of 43 origin scattered cotton genotypes under Cu excess. …”
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Strong-weak dual interface engineered electrocatalyst for large current density hydrogen evolution reaction by Shaorou Ke, Ruiyu Mi, Xin Min, Xinyu Zhu, Congyi Wu, Xin Li, Bozhi Yang, Xiaowen Wu, Yangai Liu, Zhaohui Huang, Minghao Fang

“…Abstract Supported nanocatalysts are crucial for hydrogen production, yet their activity and stability are challenging to manage due to complex metal-support interfaces. Herein, we design Pt@ anatase&rutile-TiO2 with a strong-weak dual interface by modifying TiO2 using high-energy ball milling and in-situ reduction to vary surface energies. …”
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Seaweed (Porphyra) cultivation enhances production of autochthonous refractory dissolved organic matter in coastal ecosystems by Ting Wang, Ting Wang, Jiajun Xu, Jiajun Xu, Randy A. Dahlgren, Qiang Liu, Yang Jia, Yang Jia, Binbin Chen, Binbin Chen, Hanqin Xu, Hanqin Xu, Zengling Ma, Zengling Ma, Liyin Qu, Liyin Qu

“…To fill this knowledge gap, we conducted investigations examining the quantity and optical properties of DOM and POM, microbial community structures and relevant environmental factors along a continuum from a subtropical river through its adjacent coastal Porphyra cultivation zone during the cultivation and non-cultivation periods. …”
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Reduction in <i>Aedes aegypti</i> Population After a Year-Long Application of Targeted Sterile Insect Releases in the West Valley Region of Southern California by Solomon Kibret Birhanie, Jacob Hans, Jennifer Thieme Castellon, Ale Macias, Rubi Casas, Huy Hoang, Deanna Mormile, Kecia Pitts-Love, Michelle Q. Brown

“…Over 99.6% eggs collected from female mosquitoes mated with irradiated males did not hatch, indicating a high level of sterility of the irradiated males utilized for our SIT application. Here, we demonstrated the potential of SIT when combined with IVM strategies such as In2Care^® Mosquito Stations, offering a holistic approach to reducing the public health risks associated with <i>Aedes</i>-borne diseases.…”
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Combination gemcitabine and PD-L1xCD3 bispecific T cell engager (BiTE) enhances T lymphocyte cytotoxicity against cholangiocarcinoma cells by Methi Wathikthinnakon, Piriya Luangwattananun, Nunghathai Sawasdee, Chutipa Chiawpanit, Vannajan Sanghiran Lee, Piyarat Nimmanpipug, Yingmanee Tragoolpua, Siriphorn Rotarayanont, Thanich Sangsuwannukul, Nattaporn Phanthaphol, Yupanun Wutti-in, Chalermchai Somboonpatarakun, Thaweesak Chieochansin, Mutita Junking, Jatuporn Sujjitjoon, Pa-thai Yenchitsomanus, Aussara Panya

“…To overcome this challenge and take advantage of PD-L1 upregulation upon gemcitabine treatment, we generated recombinant PD-L1xCD3 bispecific T cell engagers (BiTEs) to simultaneously block PD-1/PD-L1 signaling and recruit T lymphocytes to eliminate CCA cells. …”
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Immune-related adverse events with PD-1/PD-L1 inhibitors: insights from a real-world cohort of 2523 patients by Ting Yan, Minghui Long, Chaoyi Liu, Jiwen Zhang, Jiwen Zhang, Xingyu Wei, Xingyu Wei, Fei Li, Fei Li, Dehua Liao

“…This study aims to investigate the prevalence and associated risk factors of irAEs in a real-world setting, as well as to assess their effects on optimal therapeutic outcomes.MethodsA retrospective analysis involved 2523 patients with cancer who received inpatient PD-1/PD-L1 inhibitors treatment between January 2018 and December 2022. We documented patients’ demographic and clinical characteristics, PD-1 or PD-L1 inhibitors, treatment modalities, incidences, timing, and severity of irAEs, and efficacy outcomes by reviewing inpatient records. …”
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Body composition and physical performance of mountain bike athletes by Rayane Carvalho de Moura, Crislane de Moura Costa, Cirley Pinheiro Ferreira, Bruna Lorena Soares Cavalcante Sousa, Leandra Caline dos Santos, Michelly da Silva Pinheiro, Sérgio Luís Galan Ribeiro, Sandro Soares Almeida, Joseline Lima e Silva Pinho, Naíza Monge Lages, Maria Flávia de Sousa, Iago Alves Costa, Glêbia Alexa Cardoso, Acácio Salvador Veras Silva, Alisson Alves Silva, Jefferson Cruz dos Santos Leite, Felipe Machado Brito, Lailson Henrique Oliveira dos Santos, Rubens Lima Rodrigues, Jairo da Costa Souza, Marcos Antonio Pereira dos Santos

“…Participants performed the standing long jump and handgrip strength tests, to assess their strength of the lower and upper limbs, respectively. Finally, we measured the completion time of a Mountain Bike Marathon, with the distance of 75 km). …”
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Inverse association between obesity and suicidal death risk by Joonyub Lee, Seung-Hwan Lee, Mee-Kyoung Kim, Hyuk-Sang Kwon, Jae-Seung Yun, Yeoree Yang, Kun-Ho Yoon, Jae-Hyoung Cho, Chi-Un Pae, Kyungdo Han, Jang Won Son

“…Methods This study was nationwide, retrospective, observational study based on Korean National Health Insurance Service database. We analyzed 4,045,081 participants who were aged > 19 years and underwent national health surveillance in 2009. …”
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Advancing precision therapy for colorectal cancer: Developing clinical indications for multi-target kinase inhibitor BPR1J481 using patient-derived xenograft models by Ya-Chu Tang, Jing-Jim Ou, Shu-Ching Hsu, Chih-Hsiang Huang, Li-Mei Lin, Hsin-Huei Chang, Yi-Hsin Wang, Zih-Ting Huang, Manwu Sun, Ko-Jiunn Liu, Yi-Mei Hung, Chi-Yun Lai, Chuan Shih, Chiung-Tong Chen, Jang-Yang Chang, Hsing-Pang Hsieh, Weir-Torn Jiaang, Ching-Chuan Kuo

“…Through kinase competitive binding and kinase activity assays, we discover that BPR1J481 effectively inhibits SRC kinase activity by directly binding to its active site. …”
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Diminished ovarian reserve is associated to euploidy rate: a single center study by Edoardo Carnesi, Stefano Castellano, Stefano Castellano, Elena Albani, Andrea Busnelli, Andrea Busnelli, Antonella Smeraldi, Ozgur Bulbul, Emanuela Morenghi, Valentina Immediata, Paolo Emanuele Levi-Setti, Paolo Emanuele Levi-Setti

“…The relationship between these biomarkers and embryo chromosomal status remains complex and poorly understood.MethodsWe conducted a retrospective analysis of in vitro fertilization (IVF) cycles performed between 2015 and 2022, involving 773 female patients who underwent IVF and pre-implantation genetic screening for aneuploidy (PGT-A). …”
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