Development of SacB-based counterselection for efficient allelic exchange in Fusobacterium nucleatum by Peng Zhou, Bibek G C, Bo Hu, Chenggang Wu

“…Utilizing sucrose counterselection, we created an in-frame deletion of the F. nucleatum tonB gene, a critical gene for energy-dependent transport processes in Gram-negative bacteria, and a precise knock-in of the luciferase gene immediately following the stop codon of the hslO gene, the last gene of a five-gene operon possibly related to the natural competence of F. nucleatum. …”
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<i>Clostridium difficile</i>-associated disease by V. T. Ivashkin, O. S. Shifrin, A. S. Tertycnhy, Ye. A. Poluektova, T. L. Lapina, O. S. Lyashenko, K. V. Ivashkin

“…C.difficile is unthrifty on breeding grounds, obligate anaerobic, gram-positive, sporeforming, cytotoxin-producing bacterium. …”
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Reproductive Diseases and Disorders of Female Camels: An Assessment and Pathological and Bacteriological Study in Eastern Ethiopia by Dinaol Belina, Amare Eshetu, Sisay Alemu, Bekyad Shasho, Tajudin Mohammed, Ahmedin Mohammed, Bahar Mummed, Dereje Regassa

“…Of the 119 microbiological samples processed, 77.3% were positive for single or mixed bacterial genera, from which 7 different bacterial isolates and 14 other unidentified Gram-negative bacteria were detected. E. coli, Salmonella, and Staphylococcus spp. were the most frequently isolated organisms with 28.2%, 26.9%, and 12.8% frequencies, respectively. …”
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Microbial Contamination in Chicken Meat in Traditional Markets in Banyuwangi by Prima Ayu Wibawati, Yunia Chrismonica, Racca Biana Susanti, Mario Putra Himawan, Afaf Heny Sintya Devy, Juwita Ayu Nirmalasari, Abzal Abdramanov

“…E. coli and Salmonella spp. were identified using Gram staining, culturing, and biochemical tests. …”
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IMPORTANCE OF SOURCE CONTROL IN THE SUBGROUP OF INTRA-ABDOMINAL INFECTIONS FOR SEPTIC SHOCK PATIENTS: ANALYSIS OF 390 CASES by Uğur Önal, Deniz Akyol, Olcay Buse Kenanoğlu, Merve Mert, Dilşah Başkol, Seichan Chousein Memetali, Gamze Şanlıdağ, Cansu Bulut, Arda Kaya, Ayşe Uyan Önal, Nazlıhan Yalçın, Günel Guliyeva, Şükrü Dirik, Oğuzhan Acet, Damla Akdağ, Melike Demir, Osman Bozbıyık, Berk Göktepe, Tufan Gümüş, İlkin Çankayalı, Kubilay Demirağ, Mehmet Uyar, Hilal Sipahi, Hüseyin Aytaç Erdem, Meltem Işıkgöz Taşbakan, Bilgin Arda, Şöhret Aydemir, Sercan Ulusoy, Oğuz Reşat Sipahi

“…On the other hand, female gender (p<0.001, odds ratio(OR)=1.714-5.054, 95%CI=1.714-5.054), diabetes mellitus (p= 0.014, OR=2.284, 95%CI=1.179-4.424), carbapenem-resistant Gram-negative etiology (p=0.011, OR=4.386, 95%CI=1.398-13.759), SOFA≥10 (p<0.001, OR=3.036, 95%CI=1.802-5.114), lactate >3 mg/dl (p<0.001, OR=2.764, 95%CI=1.562-4.891) and lack of source control (p=0.001, OR=2.796, 95%CI=1.523-5.133) were significantly associated with OMM in logistic regression analysis. …”
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Pharmacokinetics of tebipenem pivoxil used in children suffering from shigellosis: a pilot study in Bangladesh by Sharika Nuzhat, Md Ridwan Islam, Syed Jayedul Bashar, Subhasish Das, Rukaeya Amin, Firdausi Qadri, Farhana Khanam, Dilruba Ahmed, Patricia B. Pavlinac, Cindy X. Zhang, Samuel L. M. Arnold, Amy Newlands, Mohammod Jobayer Chisti, Tahmeed Ahmed

“…Abstract With increasing antibiotic resistance in gram-negative bacteria, including those causing Shigellosis, evidence of safety and pharmacokinetics data on new oral antibiotics is crucial. …”
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Metabolic Blockade-Based Genome Mining of <i>Malbranchea circinata</i> SDU050: Discovery of Diverse Secondary Metabolites by Hu Yang, Xiaowei Luo, Zhuo Shang, Kunlong Li, Jian Cai, Yingying Chen, Longchao Xin, Jianhua Ju

“…Bioactivity assays demonstrated that sclerin (<b>1</b>) and (−)-mycousnine (<b>10</b>) exhibited weak antibacterial activity against Gram-positive bacteria such as <i>Staphylococcus aureus</i>, methicillin-resistant <i>Staphylococcus aureus</i> (MRSA), and <i>Bacillus subtilis</i>. …”
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Senegalin-2: A Novel Hexadecapeptide from <i>Kassina senegalensis</i> with Antibacterial and Muscle Relaxant Activities, and Its Derivative Senegalin-2BK as a Bradykinin Antagonist by Yueyang Lu, Yanguo Zhu, Chengbang Ma, Lei Wang, Mei Zhou, Tianbao Chen, Xiaonan Ma, Xu Zhang, Zhimin Fan

“…This modification retained potent activity against Gram-positive bacteria. Compared to Senegalin-2, Senegalin-2BK significantly reduced hemolysis and exhibited a more than threefold increase in the selectivity index. …”
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Community structure and diversity of myxobacteria in soils from Inner Mongolia, China by Zhihua Wu, Zhihua Wu, Songyuan Li, Xuehan Wang, Xuehan Wang, Huirong Liu

“…Myxobacteria are a special kind of Gram-negative bacteria that can slide and produce a variety of bioactive substances against bacteria, fungi, and viruses. …”
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Exploring Antibacterial Properties of Marine Sponge-Derived Natural Compounds: A Systematic Review by Cintia Cristina Santi Martignago, Camila de Souza Barbosa, Homero Garcia Motta, Beatriz Soares-Silva, Erica Paloma Maso Lopes Peres, Lais Caroline Souza e Silva, Mirian Bonifácio, Karolyne dos Santos Jorge Sousa, Amanda Sardeli Alqualo, Júlia Parisi, Olivier Jordan, Ana Claudia Muniz Renno, Anna Caroline Campos Aguiar, Viorica Patrulea

“…This systematic review examines recent advancements in identifying new sponge-derived compounds with antimicrobial activity, specifically targeting <i>Pseudomonas aeruginosa</i>, a prevalent Gram-negative pathogen with the highest incidence rates in clinical settings. …”
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Epidemiological and genomic analysis of Acinetobacter baumannii strains from retailed raw meat by Leila Hamze, Raquel Garcia-Fierro, Antoine Drapeau, Pauline François, Andrea Endimiani, Jean-Yves Madec, Marisa Haenni, Vincent Perreten, Agnese Lupo

“…While molecular epidemiology of A. baumannii is well described in hospital settings, extra-hospital settings remain poorly investigated.In the frame of two screening campaigns for the presence of Gram-negative bacteria in retailed raw meat, we collected 70 A. baumannii isolates. …”
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A Study of Trichomonas vaginalis Infection and Correlates in Women with Vaginal Discharge Referred at Fann Teaching Hospital in Senegal by Roger C. Tine, Khadime Sylla, Rougyatou Ka, Lamine Dia, Doudou Sow, Souleye Lelo, Khardiata Diallo, Babacar Faye, Thérèse Dieng, Cheikh T. Ndour, Ahmet Y. Sow

“…Optic microscopic examination with 40x magnification was done to detect T. vaginalis and assess biological modifications such as presence of epithelial cells, white blood cells, and red blood cells. A gram stained smear was also performed and examined under oil immersion (100x magnification) to assess the vaginal flora. …”
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Prevalence of Helicobacter pylori infection and its Associated Risk Factors among symptomatic Residents of Sulaimani city, Kurdistan region, Iraq, 2020 by Seerwan Hama Rashid Ali, Sabiha Sharif Salih, Taib Ahmed Hama Sour, Goran Mohammad Raouf, Araz Latif Rahim

“…Helicobacter pylori (H. pylori) bacteria are a microaerobic Gram negative that colonizes in the gastric and duodenum of human. …”
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Comparative Genomics Analysis of Plasmid pPV989-94 from a Clinical Isolate of Pantoea vagans PV989 by Lei Xu, Min Yin, Tingyuan Zhu, Yabo Liu, Yuanyuan Ying, Junwan Lu, Chaoqing Lin, Jianchao Ying, Teng Xu, Liyan Ni, Qiyu Bao, Shunfei Lu

“…Pantoea vagans, a gram-negative bacterium from the genus Pantoea and family Enterobacteriaceae, is present in various natural environments and considered to be plant endophytes. …”
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Extracellular Lipase from Pseudomonas aeruginosa SB-37: Production by Solid State Fermentation, Immobilization, and Characterization by Titin Haryati, Norman Yoshi Haryono, Dian Nugraheni, Fenti Fatmawati, Alfina Kusuma Dewi, Muhammad Zahran Edlian Syach

“…This gave a lypolitic activity value of 2 U/gds (gram dry weight substrates). Since the protein profile of extracellular lipase has a few protein bands, we perform direct immobilization on crude protein supernatant. …”
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Harmful impacts of microplastic pollution on poultry and biodegradation techniques using microorganisms for consumer health protection: A review by Mohamed E. Abd El-Hack, Elwy A. Ashour, Fatemah AlMalki, Asmaa F. Khafaga, Mahmoud Moustafa, Mohammed O. Alshaharni, Islam M. Youssef, Ahmed A. Elolimy, Sylwester Świątkiewicz

“…The contamination levels ranged from 0.03±0.04 to 1.19±0.72 particles per gram of chicken meat. In poultry, microplastics negatively affect production and harm vital organs such as the kidneys, spleen, and lungs. …”
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Assessment of the application of the FA280—a fully automated fecal analyzer for diagnosing clonorchiasis: a mixed-method study by Si-Yue Huang, Qing-Sheng Zeng, Xin-Fu Shi, Yun-Ting He, Yue-Yi Fang, Ying-Si Lai

“…Additionally, Pearson’s Chi-square test was used to analyze the consistency of positive results between the two methods across various eggs per gram (EPG) groups under different cut-off values. …”
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Characterization of the Vibrio anguillarum VaRyhB regulon and role in pathogenesis by Yingjie Li, Xinran Yu, Peng Li, Xin Li, Lushan Wang

“…BackgroundThe marine Gram-negative bacterium Vibrio anguillarum is one of the major pathogens in aquaculture. …”
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Evaluation of Airborne Particulate Matter and Volatile Organic Compounds Released by Three Types of Mosquito Repellents by Soyeon Lee, Jongmin Park, Myoungho Lee, Minjung Kim, Boowook Kim, Kiyoung Lee, Kyungduk Zoh, Chungsik Yoon

“…When normalized by usage weight, Coil types again show the highest emission factors (6.59 × 1011 particles g‒1), implying they emit more particles per gram of product burned than liquid (1.82 × 1011 particles g‒1) and mat (4.81 × 1010 particles g‒1) types. …”
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RETRACTED: Melanin Produced by Bordetella parapertussis Confers a Survival Advantage to the Bacterium during Host Infection by Yukihiro Hiramatsu, Takashi Nishida, Dendi Krisna Nugraha, Fuminori Sugihara, Yasuhiko Horiguchi

“…IMPORTANCE In addition to the Gram-negative bacterium Bordetella pertussis, the etiological agent of pertussis, Bordetella parapertussis also causes respiratory infection in humans, with a mild pertussis-like disease. …”
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