How to improve the quality of euglycemic glucose clamp tests in long-acting insulin studies by Yi Yang, Fu Kuang, XueYing Zhu, Li Li, Yao Huang, Yang Liu, Xian Yu

“…However, despite its widespread use, there remains a notable absence of an established gold standard for evaluating the test’s quality. …”
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Diagnostic Accuracy of Non-Invasive Diagnostic Tests for Nonalcoholic Fatty Liver Disease: A Systematic Review and Network Meta-Analysis by Sun Y, Hu D, Yu M, Liang SB, Zheng Y, Wang X, Tong G

“…We aimed to assess and compare the diagnostic accuracy of various NITs for NAFLD using network meta-analysis (NMA).Materials and Methods: We conducted a systematic search in seven databases up to April 2024 to identify studies evaluating the diagnostic values of NITs, with liver biopsy as the gold standard. The participants included patients with suspected or confirmed NAFLD, irrespective of age, sex, ethnicity. …”
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Surgical resection, radiotherapy and percutaneous thermal ablation for treatment of stage 1 non-small cell lung cancer: protocol for a systematic review and network meta-analysis by Arun Chockalingam, Menelaos Konstantinidis, Brandon Koo, John Timothy Moon, Andrew Tran, Sahar Nourouzpour, Emily Lawson, Kathleen Fox, Peiman Habibollahi, Bruno Odisio, Mohammed Loya, Ali Bassir, Nariman Nezami

“…Currently, surgical resection is the gold standard of treatment. However, as patients are becoming medically more complex presenting with advanced disease, minimally invasive image-guided percutaneous ablations are gaining popularity. …”
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Clinical evaluation of a multiplex droplet digital PCR for diagnosing suspected bloodstream infections: a prospective study by Yaqin Peng, Ruijie Xie, Yifeng Luo, Yifeng Luo, Yifeng Luo, Penghao Guo, Zhongwen Wu, Yili Chen, Pingjuan Liu, Jiankai Deng, Bin Huang, Kang Liao

“…The results of ddPCR testing were evaluated in comparison with blood cultures (BCs) and clinical diagnosis. Taking BC as the gold standard, receiver operating characteristic curve and Cohen’s kappa agreement were used to investigate whether the pathogen load could predict a corresponding culture-proven BSI for the top five microorganisms detected by ddPCR.ResultsOf the 173 blood samples collected, BC and ddPCR were positive in 48 (27.7%) and 92 (53.2%) cases, respectively. …”
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Effectiveness of a specialist palliative home care nurse–patient consultation followed by an interprofessional telephone case conference compared with usual care among patients wit... by Judith Dams, Hans-Helmut König, Tim Friede, Nils Schneider, Stephanie Stiel, Martin Scherer, Silke Böttcher, Gabriella Marx, Ingmar Schäfer, Nadine Janis Pohontsch, Hendrik van den Bussche, Friedemann Nauck, Michael Freitag, Eva Hummers, Tina Mallon, Franziska Schade, Manuel Zimansky, Thomas Asendorf, Christiane A Mueller

“…Patients diagnosed with CHF (New York Heart Association (NYHA) classification 3–4), COPD (Global Initiative for Chronic Obstructive Lung Disease (GOLD) stage classification 3–4, group D) or dementia GDS stage 4 or above). …”
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plaTform fOr Urinary tract infection diagnostiC evAluatioN (TOUCAN): a protocol for a prospective diagnostic accuracy study of point-of-care testing in patients suspected of acute... by Thomas R Fanshawe, Philip J Turner, Gail N Hayward, Margaret Glogowska, Alastair D Hay, Michael Moore, Mark Lown, Christopher C Butler, Mandy Wootton, Mark H Wilcox, Jane Freeman, Nicola Kenealy, Valerie Tate, Owain Llion, Rebecca Lowe

“…Susceptibility testing will be performed using ‘gold-standard’ methods, not usually performed in diagnostic laboratories. …”
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Extracellular vesicles, RNA sequencing, and bioinformatic analyses: Challenges, solutions, and recommendations by Rebecca T. Miceli, Tzu‐Yi Chen, Yohei Nose, Swapnil Tichkule, Briana Brown, John F. Fullard, Marilyn D. Saulsbury, Simon O. Heyliger, Sacha Gnjatic, Natasha Kyprianou, Carlos Cordon‐Cardo, Susmita Sahoo, Emanuela Taioli, Panos Roussos, Gustavo Stolovitzky, Edgar Gonzalez‐Kozlova, Navneet Dogra

“…Here, we address these gaps by describing the following points by (1) focusing on the large canopy of the EVs and particles (EVPs), which includes, but not limited to – exosomes and other large and small EVs, lipoproteins, exomeres/supermeres, mitochondrial‐derived vesicles, RNA binding proteins, and cell‐free DNA/RNA/proteins; (2) examining the potential functional roles and biogenesis of EVPs; (3) discussing various transcriptomic methods and technologies used in uncovering the cargoes of EVPs; (4) presenting a comprehensive list of RNA subtypes reported in EVPs; (5) describing different EV‐RNA databases and resources specific to EV‐RNA species; (6) reviewing established bioinformatics pipelines and novel strategies for reproducible EV transcriptomics analyses; (7) emphasizing the significant need for a gold standard approach in identifying EV‐RNAs across studies; (8) and finally, we highlight current challenges, discuss possible solutions, and present recommendations for robust and reproducible analyses of EVP‐associated small RNAs. …”
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Exploring the ability of plasma pTau217, pTau181 and beta-amyloid in mirroring cerebrospinal fluid biomarker profile of Mild Cognitive Impairment by the fully automated Lumipulse®... by Marcella Catania, Claudia Battipaglia, Alberto Perego, Erika Salvi, Emanuela Maderna, Federico Angelo Cazzaniga, Paolo M. Rossini, Camillo Marra, Nicola Vanacore, Alberto Redolfi, Daniela Perani, Patrizia Spadin, Maria Cotelli, Stefano Cappa, Naike Caraglia, Pietro Tiraboschi, Fabrizio Tagliavini, Giuseppe Di Fede

“…However, their ability to reflect the cerebrospinal fluid (CSF) profile - that remains to date the gold standard for the biochemical diagnosis of AD - needs to be confirmed and validated before their implementation in clinical practice. …”
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Performance of the AmpliSeq NGS panel in thyroid nodules with indeterminate cytology by Wiame Potonnier, Erell Guillerm, Claude Bigorgne, Cécile Ghander, Malanie Roy, Florence Coulet, François Ansart, Fabrice Menegaux, Laurence Leenhardt, Isabelle Brocheriou, Gabrielle Deniziaut, Camille Buffet

“…The final histological diagnosis was used as the designated gold standard. Results: Histologically, 86 nodules were benign and 35 nodules were malignant. …”
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Validity of a machine learning estimation of blood volumes during altitude training by Basile Moreillon, Bastien Krumm, Lena Mettraux, Julian Wackernell, James Spragg, Martin Faulhaber, Raphael Faiss

“…In conclusion, this study suggest that CO-rebreathing may definitely represent a gold-standard to asess the effectiveness of an altitude sojourn on increasing Hbmass while a predictive machine-learning model may allow to monitor PV shifts from a single blood sample to help decision-making regarding body fluid balance before, during and after an altitude training camp in professional cyclists. …”
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Validity, Accuracy, and Safety Assessment of an Aerobic Interval Training Using an App-Based Prehabilitation Program (PROTEGO MAXIMA Trial) Before Major Surgery: Prospective, Inter... by Sara Fatima Faqar Uz Zaman, Svenja Sliwinski, Lisa Mohr-Wetzel, Julia Dreilich, Natalie Filmann, Charlotte Detemble, Dora Zmuc, Felix Chun, Wojciech Derwich, Waldemar Schreiner, Wolf Bechstein, Johannes Fleckenstein, Andreas A Schnitzbauer

“…ObjectiveThis study aims to test the accuracy and validity of a new software in comparison to the gold-standard electrocardiogram (ECG)-based heart rate measurement. …”
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A prospective multi-site study to evaluate the performance and usability of an oral fluid-based HIV self-test in Canada by Richard A. Galli, Darshanand Maraj, Kristin McBain, Jason M. Lo Hog Tian, Abbey McFarland, Wangari Tharao, Nontobeko P. Nkala, Arlene Chan, Mark da Silva, Rejean Thomas, Anne-Fanny Vassal, Martin Lepage, Laurie Ireland, Mike Payne, Jared Starr, Chris Fraser, Marion Selfridge, Mona Loutfy, Roberta Halpenny, Nirubini Jeyarajah, Vanessa Tran, Tony Mazzulli, Sean B. Rourke

“…Positive and negative agreement was determined by comparing the results of the HIV self-tests with the results of the laboratory-based “gold standard” Abbott Alinity HIV Antigen/Antibody Combo test. …”
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Electrocardiographic signs of cardiac ischemia at rest and during exercise in patients with COPD traveling to 3,100 m: data from a randomized trial of acetazolamide by Marla Christen, Marla Christen, Aline Buergin, Aline Buergin, Maamed Mademilov, Maamed Mademilov, Laura Mayer, Laura Mayer, Simon R. Schneider, Simon R. Schneider, Mona Lichtblau, Mona Lichtblau, Talant M. Sooronbaev, Talant M. Sooronbaev, Silvia Ulrich, Silvia Ulrich, Konrad E. Bloch, Konrad E. Bloch, Michael Furian, Michael Furian

“…We assessed electrocardiogram (ECG)-derived signs of cardiac ischemia and the effects of preventive acetazolamide therapy in COPD patients traveling to high altitudes.MethodsPatients with COPD [Global Initiative for Chronic Obstructive Pulmonary Disease (GOLD) grades 2–3] and a predicted forced expiratory volume in 1 s (FEV1) of 66 ± 11% (mean ± SD), aged 57 ± 8 years, and living <1,000 m were included in this analysis of secondary outcomes from a randomized placebo-controlled double-blind trial (www.clinicaltrials.gov, NCT03156231). …”
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Perbedaan Efektivitas Parasetamol Oral Dengan Tramadol Oral Sebagai Tatalaksana Nyeri Pasca Operasi Transurethral Resection of The Prostate by Ismail Muhammad, Alvarino Alvarino, Nasman Puar, Hafni Bachtiar

“…Transurethral Resection of the Prostate (TURP) is an endoscopic surgery that become the gold standard for the treatment of benign enlargement of the prostate gland that requires surgery. …”
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Aggregation‐induced circularly polarized luminescence and delayed fluorescence enabled by activating high‐level reverse intersystem crossing by Ke Wang, Xinwen Ou, Xiaofei Niu, Zhenghao Wang, Fengyan Song, Xiaobin Dong, Wu‐jie Guo, Hui‐Qing Peng, Zujin Zhao, Jacky W. Y. Lam, Jianwei Sun, Hongkai Wu, Shu‐Yan Yu, Fei Li, Ben Zhong Tang

“…Rational manipulation of higher excited triplet state Tn (n > 1) excitons and suppression of Kasha's rule of CPL materials remains a major challenge. Herein, two gold complex enantiomers ((R/S)‐BPAuBC) based on axially chiral binaphthyls and 3,6‐Di‐tert‐butylcarbazole groups are synthesized and systematically investigated. …”
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Propolis mouthwashes efficacy in managing gingivitis and periodontitis: a systematic review of the latest findings by Muhammed Al-Huda Ballouk, Mohamed Altinawi, Abeer Al-Kafri, Talar S. Zeitounlouian, Piotr S. Fudalej

“…Conclusions Despite the fact that propolis has shown positive effects in terms of controlling gingival and periodontal inflammation especially when used with mechanical methods, studies lack certainty and their power of evidence is low with no agreed gold standards. These conclusions come, for sure, within the limitations of this review, like having substantial variability amongst the included studies and the presence of studies with a high risk of bias. …”
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A customizable multiplex protein microarray for antibody testing and its application for tick-borne and other infectious diseases by Hari Krishnan Krishnamurthy, Vasanth Jayaraman, Karthik Krishna, Tianhao Wang, Kang Bei, Chithra Changalath, Shiny Matilda, Alex J. Rai, Renata Welc-Falęciak, Agnieszka Pawełczyk, Lucas S. Blanton, Aleš Chrdle, Andrea Fořtová, Daniel Růžek, Gheyath K. Nasrallah, Laith J. Abu-Raddadi, Duaa W. Al-Sadeq, Marah Abed Alhakim Abdallah, Daniele Lilleri, Chiara Fornara, Piera D’Angelo, Milena Furione, Maria Söderlund-Venermo, Klaus Hedman, Dimosthenis Chochlakis, Eirini Makridaki, Artemis Ntoula, Anna Psaroulaki, Angélica Escárcega-Ávila, John J. Rajasekaran

“…The microarray improved IgM and IgG sensitivities and specificities of individual microbes when compared with the respective gold standards. The testing was also performed in a single run in comparison to multiple runs needed for comparable testing standards. …”
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Comparison of fluorescence in situ hybridization (FISH) and urine cytology in diagnosing urothelial carcinoma: a single-center retrospective cohort study by WANG Zhiting, ZHU Xiaoli

“…Using histopathology as the gold standard, the accuracy of FISH, cytology and their combination in diagnosing UC was 79.51%, 72.08% and 77.39%, respectively; sensitivity was 72.06%, 58.82% and 78.68%, respectively; specificity was 86.39%, 84.35% and 76.19%, respectively. …”
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Is there a mental health diagnostic crisis in primary care? Current research practices in global mental health cannot answer that question by Brandon A. Kohrt, Dristy Gurung, Ritika Singh, Sauharda Rai, Mani Neupane, Elizabeth L. Turner, Alyssa Platt, Shifeng Sun, Kamal Gautam, Nagendra P. Luitel, Mark J.D. Jordans

“…We recommend evaluating multiple conditions, statistically adjusting prevalence estimates generated from self-report tools, and consistently using structured clinical interviews as a gold standard. We propose clinically meaningful detection as ‘good-enough’ diagnoses incorporating multiple conditions accounting for context, health system and types of interventions available. …”
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A New Smartphone-Based Cognitive Screening Battery for Multiple Sclerosis (icognition): Validation Study by Stijn Denissen, Delphine Van Laethem, Johan Baijot, Lars Costers, Annabel Descamps, Ann Van Remoortel, Annick Van Merhaegen-Wieleman, Marie D’hooghe, Miguel D'Haeseleer, Dirk Smeets, Diana M Sima, Jeroen Van Schependom, Guy Nagels

“…Test performance was comparable between people with MS and healthy participants for all cognitive tests, both in icognition (Symbol Test: U=4431; P=.42; Dot Test: U=3516; P=.32; vBDS: U=3708; P=.27) and the gold standard paper-and-pencil tests (Symbol Digit Modalities Test: U=4060.5, P=.82; 10/36 Spatial Recall Test: U=3934; P=.74; auditory Backward Digit Span: U=3824.5, P=.37). …”
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