Impact of viral load on sample pooling for reverse-transcription polymerase chain reaction detection-based diagnosis of coronavirus disease 2019 in Nigeria by Timan T. Eliya, Elvis E. Isere, Bassey Emmana, Chukwuebuka Ugwu, Jonathan Kushim, Precious Ishaku, Aisha E. Ibrahim, John S. Bimba

“…Reverse-transcription polymerase chain reaction (RT-PCR) remains the gold standard for COVID-19 detection, but limited testing resources caused bottlenecks in Nigeria’s response during the pandemic. …”
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Culture-independent detection of Mycobacterium tuberculosis complex DNA using targeted next generation sequencing in African buffalo (Syncerus caffer) oronasal swabs in South Afric... by Sinegugu Kholeka Mhlophe, Charlene Clarke, Giovanni Ghielmetti, Giovanni Ghielmetti, Megan Matthews, Tanya Jane Kerr, Michele Ann Miller, Wynand Johan Goosen, Wynand Johan Goosen

“…Controlling aTB in South Africa relies on mycobacterial culture as the gold standard for M. bovis confirmation, with the single intradermal comparative cervical test (SICCT) and Bovigam™ assays as validated cell-mediated immunological assays for detection. …”
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Noninvasive Detection of Alpha-Amylase in Saliva Using Screen-Printed Carbon Electrodes: A Promising Biomarker for Clinical Oral Diagnostics by Reviansyah FH, Ristin AD, Rauf AA, Sepirasari PD, Alim FN, Nur Y, Takarini V, Yusuf M, Aripin D, Susilawati S, Komariah M, Alam BYCSSS

“…Saliva samples are applied directly onto electrodes pre-coated with biomarkers and a conditioning agent, allowing for precise detection and analysis.Results: The screen-printed carbon electrode demonstrated excellent performance in detecting alpha-amylase, with clear voltammogram results, achieving a limit of detection (LOD) of 104.252 units and a limit of quantification (LOQ) of 315.915 units.Conclusion: A gold nanoparticle-modified screen-printed electrode (SPE) was developed to measure alpha-amylase quantitatively. …”
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Low-Dose Ketamine Infusion for Perioperative Pain Management in Patients Undergoing Laparoscopic Gastric Bypass: A Prospective Randomized Controlled Trial by Mitchell T. Seman, Shawn H. Malan, Matthew R. Buras, Richard J. Butterfield, Kristi L. Harold, James A. Madura, David M. Rosenfeld, Andrew W. Gorlin

“…Laparoscopic Roux-en-Y gastric bypass is the gold standard surgical treatment for patients whose weight is refractory to diet and exercise. …”
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Use of Blood Lipid Indicators as a Screening Tool of Insulin Resistance among Individuals in Low-Income Country Sides of China: A Multiethnic Region Study by Yi-Zhong Yan, Jia-Ning Fan, Jia-Ming Liu, Yun-hua Hu, Jiao-Long Ma, Jia He, Heng Guo, Xiang-hui Zhang, Xin-ping Wang, Shu-gang Li, Shu-Xia Guo

“…The homeostasis model assessment was the gold standard for identifying IR. The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value, and the nomogram was utilized to analyze the predictive value. …”
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Could kDNA-PCR in Peripheral Blood Replace the Examination of Bone Marrow for the Diagnosis of Visceral Leishmaniasis? by Natalia Souza de Godoy, Marcos Luiz Alves Andrino, Regina Maia de Souza, Erika Gakiya, Valdir Sabbaga Amato, José Ângelo Lauletta Lindoso, Lucia Maria Almeida Braz

“…PB from suspected VL patients was evaluated by parasitological and molecular techniques using as the gold standard (GS) a combination of clinical, epidemiological, and immunochromatographic test (PB-rK39) results and parasitological examination of BM. …”
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An electrolyte-gated transistor for the monitoring of a CRISPR/Cas activity by Pierre Guermonprez, Le Tu Anh, Louis Renaud, Pierre Nioche, Eric Krejci, Aurélie Alleaume-Butaux, Nicolas Battaglini, Vu Thi Thu, Sébastien Sanaur, Benoît Piro

“…Reverse transcriptase-polymerase chain reaction (RT-PCR) remains the gold standard, but clustered regularly interspaced short palindromic repeats linked to a Cas endoribonuclease particle (CRISPR/Cas) have recently revolutionized the recognition step of two RNAs, i.e. the CRISPR-RNA (crRNA) and the target, providing a much better selectivity compared to the naked hybridization on which RT-PCR is based. …”
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Blue-LED assisted Photodegradation kinetics of rhodamine-6G dye, enhanced anticancer activity and cleavage of plasmids using Au-ZnO nanocomposite by Pilavadi Thangamuniyandi, Devan Umapathy, Loganathan Nagarajan, Antony Joseph Velanganni Arockiam

“…Herein, a hexagonal rod-shaped ZnO nanoparticles (NP) precursor was prepared using the sol-gel method, which in the presence of varying concentrations of gold (0.005M, 0.01M, and 0.015M) via a greener citrate reduction method afforded a nanocrystalline Au-ZnO nanocomposite. …”
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Clinically relevant orthotopic pancreatic cancer models for adoptive T cell transfer therapy by Gregory B. Lesinski, Chrystal M. Paulos, Maggie Phillips, Jacklyn Hammons, Isaac Karpovsky, Zaid Mahdi, Natalie K. Horvat, Megan M. Wyatt, Margaret A. Hall, Cameron J. Herting, Richard A. Moffitt

“…Currently, genetically engineered mouse models (GEMMs) for PDAC are considered a “gold-standard” as they recapitulate many aspects of human disease. …”
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Integrated Evaluation of Age-Related Changes in Structural and Functional Vascular Parameters Used to Assess Arterial Aging, Subclinical Atherosclerosis, and Cardiovascular Risk in... by Daniel Bia, Yanina Zócalo, Ignacio Farro, Juan Torrado, Federico Farro, Lucía Florio, Alicia Olascoaga, Javier Brum, Walter Alallón, Carlos Negreira, Ricardo Lluberas, Ricardo L. Armentano

“…Subjects (n=388) were submitted to non-invasive vascular studies (gold-standard techniques), to evaluate (1) common (CCA), internal, and external carotid plaque prevalence, (2) CCA intima-media thickness and diameter, (3) CCA stiffness (percentual pulsatility, compliance, distensibility, and stiffness index), (4) aortic stiffness (carotid-femoral pulse wave velocity), and (5) peripheral and central pressure wave-derived parameters. …”
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Artificial intelligence-assisted platform performs high detection ability of hepatocellular carcinoma in CT images: an external clinical validation study by Rongxue Shan, Chenhao Pei, Qianrui Fan, Junchuan Liu, Dawei Wang, Shifeng Yang, Ximing Wang

“…The CT images were annotated and confirmed by the experienced radiologists using InferScholar software as the “gold standard”. The automatic HCC segmentation performed by the AI-assisted platform was used to compare with the annotation of radiologists. …”
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Establishment and application of a rapid new detection method for antimicrobial susceptibility testing of Klebsiella pneumoniae based on MALDI-TOF MS by Yapei Zhang, Fanghua Fan, Xuan Wang, Jie Zhu, Shilei Dong

“…Guidelines recommend targeted therapy based on minimum inhibitory concentration results, which directly reflect the effectiveness of antibacterial drugs. The gold standard method of AST relies on visible bacterial growth, causing long turnaround times. …”
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Prevalence, incidence and determinants of QuantiFERONTM positivity in South African schoolchildren by J. Stewart, N. Walker, K. Jennings, C. Delport, J Nuttall, A.K. Coussens, R Dyers, D.A. Jolliffe, J.C.Y. Tang, W.D. Fraser, R.J. Wilkinson, L.-G. Bekker, A.R. Martineau, K. Middelkoop

“…Prevalent infection was defined as QuantiFERON™-TB Gold Plus (QFT-Plus) positivity as assessed at baseline. …”
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Comparison of Diaphragmatic Stretch Technique and Manual Diaphragm Release Technique on Diaphragmatic Excursion in Chronic Obstructive Pulmonary Disease: A Randomized Crossover Tri... by Aishwarya Nair, Gopala Krishna Alaparthi, Shyam Krishnan, Santhosh Rai, R. Anand, Vishak Acharya, Preetam Acharya

“…This randomised crossover trial included 20 clinically stable patients with mild and moderate COPD classified according to the GOLD criteria. The patients were allocated to group A or group B by block randomization done by primary investigator. …”
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Factors influencing type 2 diabetes self-management practices in rural Bangladesh: a qualitative investigation by Hasina Akhter Chowdhury, Hasina Akhter Chowdhury, Baki Billah, Shamia Akther Dipa, Ashraful Kabir, A. K. M. Fazlur Rahman, Liaquat Ali, Anju E. Joham, Anju E. Joham, Cheryce L. Harrison, Cheryce L. Harrison

“…Diabetes self-management practices (DSMP) are the gold-standard treatment approach, yet uptake remains challenge in LMICs.Purpose of the studyThis study aimed to explore the barriers to and facilitators of DSMP and preferences for intervention design and delivery in Bangladesh, an LMIC, with prevalent T2DM.MethodsSixteen qualitative focus group discussions (FGDs) with adults with T2DM and their caregivers were conducted in rural Bangladesh to explore preferences, barriers, and facilitators for community DSMP-related intervention programs. …”
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Mitochondria-targeted photothermal-chemodynamic therapy enhances checkpoint blockade immunotherapy on colon cancer by Benchao Zheng, Hongbo Wang, Shiyi Zhai, Jiangsheng Li, Kuangda Lu

“…Herein, we constructed a multifunctional nanoplatform that enables mitochondria-targeted photothermal-chemodynamic combination therapy by conjugating indocyanine green-thiol (ICG-SH) and mercaptoethyl-triphenylphosphonium (TPP-SH) onto polyvinyl pyrrolidone (PVP)-coated gold-copper nanoparticles (AIT). Upon near-infrared light (NIR) irradiation, AIT ablates cancer cells and amplifies the effect of chemodynamic therapy (CDT), thereby inducing apoptosis in the tumor. …”
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Validation of insurance claims data on aetiology, surgical type and prosthesis for isolated valve surgery: a retrospective observational surgery by Shao-Wei Chen, Victor Chien-Chia Wu, Pao-Hsien Chu, An-Hsun Chou, Chun-Yu Chen, Feng-Cheng Chang, Yi‐Hsin Chan, Chia-Pin Lin, Yu-Ting Cheng, Bo-Cheng Hou, Jo-Ting Huang

“…This study validated the coding of valvular heart repair and replacement surgeries in Taiwan’s National Health Insurance (NHI) Research Database using International Classification of Diseases, Clinical Modification (ICD-CM) codes.Methods This retrospective observational study used data from the Chang Gung Research Database between 2015 and 2018, identifying 1171 patients using Taiwanese NHI reimbursement codes. The gold standard was defined as a blinded retrospective review of operation notes. …”
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Shaping Ability of XP Endo Shaper File in Curved Root Canal Models by Abdulmohsen Alfadley, Abdalrhman Alrajhi, Hamad Alissa, Faisal Alzeghaibi, Lubna Hamadah, Khalid Alfouzan, Ahmed Jamleh

“…The aim of this study was to assess the shaping ability of the XP Shaper (XPS) file in severely curved canal models under simulated body temperature and compare it with that of the WaveOne Gold (WOG) file. Ninety-six simulated root canals were equally distributed into XPS and WOG systems to be shaped by eight files each. …”
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The Lipo-DIEP Flap Breast Reconstruction: A Valuable Innovation for Maximizing Abdominal Tissue Volume by Benedetto Longo, MD, PhD, Gennaro D’Orsi, MD, Alessio Farcomeni, PhD, Martina Giacalone, MD, Elettra Gagliano, Lisa Vannucchi, MD, Maximilian Catenacci, MD, Gianluca Vanni, MD, Claudio Oreste Buonomo, MD, Valerio Cervelli, MD

“…The deep inferior epigastric perforator (DIEP) flap is universally considered the gold standard technique for breast reconstruction (BR), though it cannot always be proposed to patients with insufficient donor-site volume. …”
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Prospective clinical performance of CoVarScan in identifying SARS-CoV-2 Omicron subvariants by Kenneth Zhu, Manoj Sah, Lenin Mahimainathan, Yan Liu, Chao Xing, Karen Roush, Andrew Clark, Jeffrey SoRelle

“…The ability to identify variants with high fidelity and low turnaround time is important both epidemiologically and clinically for pandemic monitoring and therapeutic monoclonal antibody (mAb) selection. Currently, the gold-standard test for this task is whole-genome sequencing (WGS), which is prohibitively expensive and/or inaccessible due to equipment requirements for many laboratories. …”
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