The importance of genotyping within the climate-smart plant breeding value chain – integrative tools for genetic enhancement programs by Ana Luísa Garcia-Oliveira, Rodomiro Ortiz, Fatma Sarsu, Søren K. Rasmussen, Paterne Agre, Asrat Asfaw, Moctar Kante, Subhash Chander

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Passive infusion of an S2-Stem broadly neutralizing antibody protects against SARS-CoV-2 infection and lower airway inflammation in rhesus macaques. by Christopher T Edwards, Kirti A Karunakaran, Elijah Garcia, Nathan Beutler, Matthew Gagne, Nadia Golden, Hadj Aoued, Kathryn L Pellegrini, Matthew R Burnett, Christopher Cole Honeycutt, Stacey A Lapp, Thang Ton, Mark C Lin, Amanda Metz, Andrei Bombin, Kelly Goff, Sarah E Scheuermann, Amelia Wilkes, Jennifer S Wood, Stephanie Ehnert, Stacey Weissman, Elizabeth H Curran, Melissa Roy, Evan Dessasau, Mirko Paiardini, Amit A Upadhyay, Ian N Moore, Nicholas J Maness, Daniel C Douek, Anne Piantadosi, Raiees Andrabi, Thomas R Rogers, Dennis R Burton, Steven E Bosinger

“…We observed a significant reduction in inflammatory cytokines and macrophages within the lower airway of animals infused with 10mg/kg and 1mg/kg doses of CC40.8. Viral genome sequencing demonstrated a lack of escape mutations in the CC40.8 epitope. …”
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Characterization of the Cannabis sativa glandular trichome epigenome by Lee J. Conneely, Bhavna Hurgobin, Sophia Ng, Muluneh Tamiru-Oli, Mathew G. Lewsey

“…Conclusion We report the first genome-wide histone post-translational modification maps for C. sativa glandular trichomes, and more broadly for glandular trichomes in plants. …”
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Binding affinity screening of polyphenolic compounds in Stachys affinis extract (SAE) for their potential antioxidant and anti-inflammatory effects by Hun Hwan Kim, Se Hyo Jeong, Min Yeong Park, Pritam Bhangwan Bhosale, Abuyaseer Abusaliya, Sang Joon Lee, Jeong Doo Heo, Hyun Wook Kim, Je Kyung Seong, Dong Il Kim, Kwang Il Park, Gon Sup Kim

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Long Noncoding RNA HCG9 Promotes Osteosarcoma Progression through RAD51 by Acting as a ceRNA of miR-34b-3p by Lu Wang, ShuangQing Li, Lin Qi, Lin Ling

“…The candidate lncRNA was further verified by qRT-PCR, and the association of gene expression with clinicopathological features was evaluated by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The targeting miRNA was identified using starBase analysis, and the competing endogenous RNA (ceRNA) network was established by STRING. …”
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Staphylococcus aureus carriage is associated with microbiome composition in the nares and oropharynx, not the hand, of monozygotic twins by Mark R. Dalman, W. Brian Simison, Danny Nielsen, Sabana Bhatta, Noor Ramahi, Clair Yee, Dipendra Thapaliya, Jhalka Kadariya, Shanice Cheatham, Hailee Olson, Tara C. Smith

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Clinical Value and Potential Mechanism of miRNA-33a-5p in Lung Squamous Cell Carcinoma by Xiang-Ming Wang, Shang-Wei Chen, Gang Chen, Hua-Fu Zhou, Ting-Qing Gan, Jing-Jing Zeng, Zu-Yun Li

“…The differentially expressed genes (DEGs) of LUSC and the candidate target genes of miRNA-33a-5p were overlapped to acquire a set of specific genes for further analyses of the Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Ontology (GO), and protein–protein interaction (PPI) network. miRNA-33a-5p overexpressed in LUSC was supported by 706 LUSC and 261 non-LUSC samples gathering from RT-qPCR, miRNA-seq, and public miRNA microarrays. …”
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Comprehensive Analysis of the Mechanism of Anoikis in Hepatocellular Carcinoma by Dongqian Li, Qian Bao, Shiqi Ren, Haoxiang Ding, Chengfeng Guo, Kai Gao, Jian Wan, Yao Wang, MingYan Zhu, Yicheng Xiong

“…This paper’s data (TCGA-HCC) were retrieved from the database of the Cancer Genome Atlas (TCGA). Differential gene expression with prognostic implications for anoikis was identified by performing both the univariate Cox and differential expression analyses. …”
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Virulence-associated variants in Cryptococcus neoformans sequence type 93 are less likely to be associated with population structure compared to independent rare mutations by Katrina M. Jackson, Kisakye Diana Kabbale, Marissa Macchietto, David Meya, Peter Tiffin, Kirsten Nielsen

“…Recent evidence from genome-wide association studies shows that small mutations in key virulence-associated genes are associated with the virulence of specific isolates. …”
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Protocols for the application of human embryonic stem cell-derived neurons for aging modeling and gene manipulation by Hui Zhang, Shuhui Sun, Juan Carlos Izpisua Belmonte, Guang-Hui Liu, Si Wang, Weiqi Zhang, Jing Qu

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The frequency of mutations in advanced thyroid cancer in Japan: a single-center study by Soji Toda, Hiroyuki Iwasaki, Yoichiro Okubo, Hiroyuki Hayashi, Mei Kadoya, Hiroyuki Takahashi, Tomoyuki Yokose, Yukihiko Hiroshima, Katsuhiko Masudo

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Study of the Phytochemical Composition, the Antioxidant and the Anti-Inflammatory Effects of Two Sub-Saharan Plants: Piliostigma reticulatum and Piliostigma thonningii by K. Boualam, B. Ndiaye, H. Harhar, M. Tabyaoui, N. Ayessou, K. Taghzouti

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Mediating Mendelian randomization in the proteome identified potential drug targets for obesity-related allergic asthma by Jiannan Lin, Shuwen Lu, Xiaoyu Zhao

“…We further explored the functions and enriched pathways of the mediating proteins using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. Finally, we conducted drug-targeted MR analysis to evaluate the potential of each mediator plasma proteins as a drug target gene. …”
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Diversity Assessment of Some Sesame (Sesamum indicum L.) Genotypes Cultivated in Northern Ghana Using Morphological and Simple Sequence Repeat (SSR) Markers by Raphael Adu-Gyamfi, Ruth Prempeh, Issahaku Zakaria

“…Molecular genetic diversity was assessed by using thirty eight SSR markers widely distributed across sesame genome to characterize the materials. Twenty-one out of the 38 primers were polymorphic. …”
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Leveraging the fundamentals of heat transfer and fluid mechanics in microscale geometries for automated next-generation sequencing library preparation by Olivia Ott, Sabrina Tolppi, Jennifer Figueroa-Cruz, Khaliun Myagmar, Khulan Unurbuyan, Anubhav Tripathi

“…All sequencing data from our libraries attained Phred (Q) scores > 30, mapping to reference genomes at 99% confidence. The method achieved final library concentrations and size distributions comparable with the conventional manual approach, demonstrating compatibility with downstream sequencing and subsequent data analysis. …”
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Detection of Delafloxacin Resistance Mechanisms in Multidrug-Resistant <i>Klebsiella pneumoniae</i> by András Kubicskó, János Juhász, Katalin Kamotsay, Dora Szabo, Béla Kocsis

“…Four delafloxacin-resistant <i>K. pneumoniae</i> strains were selected for whole-genome sequencing (WGS). <b>Results:</b> The MIC50 values for the 43 <i>K. pneumoniae</i> strains were as follows: ciprofloxacin 0.5 mg/L, levofloxacin 0.25 mg/L, moxifloxacin 0.5 mg/L, and delafloxacin 0.25 mg/L. …”
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Peripheral blood miRNAs are associated with airflow below threshold in children with asthma by Anshul Tiwari, Brian D. Hobbs, Rinku Sharma, Jiang Li, Alvin T. Kho, Sami Amr, Juan C. Celedón, Scott T. Weiss, Craig P. Hersh, Kelan G. Tantisira, Michael J. McGeachie

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Network Pharmacology and Molecular Docking Study on the Mechanism of the Therapeutic Effect of Strychni Semen in NSCLC by He Geng, Yujie Xue, Binghua Yan, Zhaoxue Lu, Hengjin Yang, Peng Li, Jundong Zhou

“…Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were carried out via the Metascape database. …”
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Identification of Survival Risk and Immune-Related Characteristics of Kidney Renal Clear Cell Carcinoma by Xiaobin Wu, Yonghui Liang, Xian Chen, Xiangyang Long, Wujun Xu, Li Liu, Binhui Wang, Xiong Zou

“…A series of information such as RNA sequence, clinical data, and tumor mutation burden (TMB) of KIRC patients were downloaded through The Cancer Genome Atlas (TCGA). Next, combining the survival information and gene expression data of TCGA and Gene Expression Omnibus (GEO), we established an immune gene-related prognosis model (IGRPM) and analyzed it. …”
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The role of cyclooxygenase-2 (COX-2) and inflammatory markers in the progress of Alport syndrome in Egyptian children by Moushira Zaki, Hisham A. Orban, Mohamed A. Shahba, Rehab S. I. Moustafa, Ahmed Adel, Fatina I. Fadel, Abeer Selim, Hala T. El-Bassyouni, Eman R. Youness

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