Voriconazole Pharmacokinetics Administered at 4 mg/kg IM and IV in Nursehound Sharks (<i>Scyliorhinus stellaris</i>) Under Human Care by Daniela Cañizares-Cooz, Daniel García-Párraga, Sonia Rubio-Langre, Teresa Encinas, Pablo Morón-Elorza

“…However, scientific knowledge regarding azole pharmacokinetics (PK) in fish remains limited, and despite the recent publication of a PK study with voriconazole in rays, there are still no published PK studies for azoles in sharks. …”
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Study on the protective effect of naringin-regulated lncRNA GAS5 against mitochondrial apoptosis in osteoarthritic chondrocytes by LIN Qin, ZHU Yingkai, LIN Yanming, LAN Shujie, CHEN Changxing, FU Changlong

“…In vitro experiments, Lenti-lncRNA GAS5 was used to infect sodium nitroprusside (SNP)-induced chondrocytes, and the expression of lncRNA GAS5 was detected by fluorescence in situ hybridization in rat chondrocytes; after silencing of lncRNA GAS5, the expression level of lncRNA GAS5 in rat chondrocytes was detected by Real-time PCR; Western blot detected the protein levels of Caspase-3, Bax, MMP-13 and Cyt-C.Results① Pathological changes of cartilage tissue: The articular cartilage matrix in naringenin group was relatively complete, the surface was relatively smooth, the structure was clear, the tide line was more complete, the number of chondrocytes was more full, the arrangement was more orderly, the cartilage staining was more uniform, and the structure was significantly restored than that in the model group. ② lncRNA GAS5 expression in cartilage tissue: Compared with model group, lncRNA GAS5 expression in naringin group was decreased (<italic>P</italic>&lt;0.01). ③ Expression of proteins related to mitochondrial apoptosis in cartilage tissues: Compared with model group, the expressions of Caspase-3, Bax, MMP-13 and Cyt-C in cartilage tissues of naringin group were decreased (<italic>P</italic>&lt;0.01). ④ Fluorescence expression of lncRNA GAS5 in chondrocytes: FISH results showed that the fluorescence expression of lncRNA GAS5 was decreased after naringin treatment of SNP-induced chondrocytes (<italic>P</italic>&lt;0.01). ⑤ The protein expressions of Caspase-3, Bax, MMP-13 and Cyt-C in chondrocytes: Compared with the model group, the protein expressions of Caspase-3, Bax, MMP-13 and Cyt-C in naringin group were significantly decreased (<italic>P</italic>&lt; 0.01). …”
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Human exploitation of adult horseshoe crab and public awareness campaign in Hong Kong SAR, China by Pui Yi Tang, Hei Wut Fong, Shan Yee Lee, Wing Tung Chang, Lee Yan Pang, Tin Yan Hui, Kelvin Jun Yin So

“…The market survey recorded a total of 80 adult horseshoe crabs for sale in seafood restaurants or fish stalls out of the 98 shops surveyed. In addition, phone interviews revealed a low level of awareness among Hong Kong citizens (N = 1005 respondents), with 53 % having no prior knowledge and never heard of “horseshoe crab”. …”
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The Clinicopathological Features and Prognostic Significance of HER2-Low in Early Breast Tumors Patients Prognostic Comparison of HER-Low and HER2-Negative Breast Cancer Stratified... by Sanaa Gamrani, Laila Akhouayri, Sara Boukansa, Mehdi Karkouri, Hinde El Fatemi

“…A new nomenclature has been proposed for HER2 1+ and HER2 2+ tumors that are confirmed negative according to fluorescence in situ hybridization (FISH). These tumors are now referred to as HER2-low, and it is thought that they may represent a distinct subtype of breast cancer that warrants further investigation. …”
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The Vibrio type VI secretion system induces intestinal macrophage redistribution and enhanced intestinal motility by Julia S. Ngo, Piyush Amitabh, Jonah G. Sokoloff, Calvin Trinh, Travis J. Wiles, Karen Guillemin, Raghuveer Parthasarathy

“…Using a zebrafish-native Vibrio and live imaging of larval fish, we find evidence that immune cells mediate the connection between bacteria and host mechanics. …”
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Adult dietary patterns and their association with iodine nutrition levels and thyroid function: a cross-sectional study by Gulinaizeer Abuduwaili, Jia Huang, Yan Ma, Hongguang Sun

“…The dietary patterns were PCA1 (staple food pattern), PCA2 (fruit, vegetable and meat pattern), PCA3 (fish, shrimp and legume pattern) and PCA4 (dairy-based protein pattern). …”
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Human Umbilical Cord Perivascular Cells Prevent Tumor Growth in a Melanoma Tumor-Bearing Mouse Model and Modulate Breast Cancer and Melanoma Cells in a Cell Line-Dependent Manner I... by Lianet Lopez, Hannah Shuster-Hyman, Eden Marco, Hasna Khan, Avishai Gasner, Aleksandra Uzelac, Brandon Wyse, Poonam Mander, Mugundhine Sangaralingam, Joseph Fish, Ariel Gorodensky, Samar Mouazz, Amanda Kauffman, Denis Gallagher, Andrée Gauthier-Fisher, Clifford L. Librach

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HER2 与膀胱尿路上皮癌临床病理分子分型的相关性 by 王婧婧, 吴景, 尹恬恬, 何杰

“…所有 UBC 肿瘤组织采用免疫组织化学（IHC）检测HER2 蛋白表达，HER2 表达2+的肿瘤组织采用荧光原位杂交（FISH）检测 HER2 基因扩增状态。50例浸润性UBC 肿瘤组织采用IHC 检测管腔型标记（CK20、GATA3）、基底/鳞状型标记（CK14、CK5/6）和Ki67 的蛋白表达。…”
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Marigold: a machine learning-based web app for zebrafish pose tracking by Gregory Teicher, R. Madison Riffe, Wayne Barnaby, Gabrielle Martin, Benjamin E. Clayton, Josef G. Trapani, Gerald B. Downes

“…We found differences in the number and vigor of swimming bouts between fed and unfed fish at both time points, as well as interactions between developmental stage and feeding regimen. …”
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The Used of Paci-Paci Leaves Extract Leucas sp. to Prevent Mycotic Disease by Sri Nuryati, M.A. Suparman, Y. Hadiroseyani

“… Injury of fish caused by handling of fry transportation and harvesting could impact mycosis. …”
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Physical Inactivation of Monodon Baculovirus (Mbv), a Pathogenic Virus of Tiger Prawn (Penaeus Monodon Fab.) by M. Alifuddin

“…Experimental shrimp were reared at PSIK (Pusat Studi Ilmu Kelautan), Jakarta and examined histologically for MBV infection at Lab. of Fish Health, Department of Aquaculture Faculty of Fisherise IPB.  …”
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Kidney and liver histopathology of sea bass Lates calcarifer infected with black body syndrome-associated bacteria by Sri Nuryati, Mauliza Yanti, Rahman, Sutiastuti Wahyuwardani

“…The results showed that fish liver and kidney organs in all treatments experienced necrosis compared to the control treatment, but not significantly different between bacterial infection treatments. …”
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Bone formation and growth of climbing perch Anabas testudinieus larvae fed with Zn enriched Artemia nauplii by Ismarica Ismarica, Mia Setiawati, Dedi Jusadi, Muhammad Agus Suprayudi

“…The results showed that the application of Artemia sp. enrichment at 0.1 mg Zn/mL influenced the bone formation, increased the growth, and improved the fish survival of climbing perch larvae. Feeding with 0.1 mg/mL Zn enriched Artemia nauplii could be recommended as a strategy to improve the bone formation and growth performance of climbing perch larvae.   …”
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Effects of Iron and Vitamin C on Growth Performance, Iron Utilization, Antioxidant Capacity, Nonspecific Immunity, and Disease Resistance to Aeromonas hydrophila in Chinese Mitten... by Ying Song, Xinyu Cai, Xianyong Bu, Shubin Liu, Mingqi Song, Yiwen Yang, Xiaodan Wang, Qingchao Shi, Jianguang Qin, Liqiao Chen

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CRISPR/Cas9 mutagenesis reveals an essential role of PI4KB in promoting growth and resisting hemorrhagic disease caused by GCRV-II infection in juvenile grass carp by Jie Zhang, Liqiang Zhang, Xiaoman Wu, Minhui Tao, Yang Chen, Mingxian Chang

“…Few studies have reported obtaining grass carp resistant to hemorrhagic disease via gene editing in commercial fish. Here, we demonstrate that the expression and activity of grass carp PI4KB (gcPI4KB) are vital for GCRV-I and GCRV-II replication. …”
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Application of selection Index for enhancing resistance to Cryptocarya irritans and Vibrio alginolyticus in large yellow croaker by Jiaying Wang, Jiaying Wang, Ji Zhao, Ji Zhao, Bo Liu, Hongshu Chi, Pengxing Jiang, Pengxing Jiang, Junzu Xiao, Junzu Xiao, Zhou Jiang, Zhou Jiang, Lingwei Miao, Lingwei Miao, Qian He, Qian He, Junjia Zeng, Junjia Zeng, Qiaozhen Ke, Qiaozhen Ke, Tao Zhou, Tao Zhou, Peng Xu, Peng Xu

“…Despite the high cost of genotyping and the limited economic return per individual that restricts the use of genome selection technology in aquatic species breeding, integrating multiple traits in a selection index can reduce costs and present a promising direction for fish breeding. The aim of this study was to evaluate the feasibility and performance of breeding for dual resistance traits against C. irritans and V. alginolyticus in large yellow croaker, using a selection index approach. …”
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Multi-omics analysis reveals the interplay between intratumoral bacteria and glioma by Ting Li, Zhanyi Zhao, Meichang Peng, Lu Zhang, Cheng Wang, Feiyang Luo, Meiqin Zeng, Kaijian Sun, Zhencheng Fang, Yunhao Luo, Yugu Xie, Cui Lv, Jiaxuan Wang, Jian-Dong Huang, Hongwei Zhou, Haitao Sun

“…We employed immunohistochemistry, multicolor immunofluorescence, and fluorescence in situ hybridization (FISH) staining to observe the presence and location of bacteria. …”
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CeRNA profiling and the role in regulating gonadal development in gold pompano by Changcan Yang, Yijun Zhao, Wendong Zhao, Hai Huang, Quanqi Zhang, Jinxiang Liu

“…Consequently, it has prompted researchers to investigate gonadal development process of this fish. To gain further insight into the function of competing endogenous RNA (ceRNA) in the gonads of T. ovatus and the regulatory mechanism of the ceRNA network, whole transcriptome libraries were constructed from the testes and ovaries. …”
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Analysis Model of Giant Prawns Population (Macrobrachium rosenbergii) in Estuary Edge of Sembakung Waters, Nunukan, Indonesia by Agus Indarjo, Gazali Salim, Christine Dyta Nugraeni, Lukman Yudho Prakoso, Permana Ari Soejarwo, Rukisah Rukisah, Yen Thi Hong Pham, Achmad Daengs GS, Hariyadi Hariyadi, Abdul Jabarsyah

“…The sampling was by using a survey method with purposive judgment sampling based on a catch of 14 fishermen who used the bottom fish pot from Sembakung waters, Nunukan Regency (Indonesia). …”
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Analysis of egg quality and growth-related factors during embryogenic development of olive flounder (Paralichthys olivaceus) fed different broodstock diets by Ji-Sung Moon, Minji Seong, Si-Chan Kim, Josel Cadangin, Ji-Hye Lee, Eun-Seo Lee, Bong-Seung Seo, Haham Kim, Su-Jin Park, Jukyoung Kim, Jinho Lee, Seunghyung Lee, Youn-Hee Choi

“…The current study aimed to demonstrate the comparative effect of a formulated extruded pellet (EP) against a raw fish-based moist pellet (MP) as broodstock diet of olive flounder (Paralichthys olivaceus) on the egg quality and level of growth-related factors during the embryonic development of fertilized eggs. …”
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