Culture dependent and independent approaches reveal the role of specific bacteria in human skin aging by Jing‐jing Xia, Qian Zhong, Zhi‐ming Li, Qing‐zhen Wei, Liu‐yi‐qi Jiang, Cheng Duan, Hui‐jue Jia, Yi‐mei Tan, Lian‐yi Han, Jean Krutmann, Jiucun Wang, Xiao Liu

“…Moraxella osloensis isolated from healthy skin regulates collagen metabolism and extracellular matrix assembly, and promotes cell senescence in human keratinocytes and fibroblasts, making it potentially applicable in the development of antiaging interventions.…”
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Hemodynamic disturbance and mTORC1 activation: Unveiling the biomechanical pathogenesis of thoracic aortic aneurysms in Marfan syndrome by Ming-Yuan Liu, Meili Wang, Junjun Liu, An-Qiang Sun, Chang-Shun He, Xin Cong, Wei Kong, Wei Li

“…Therapeutic administration of rapamycin suppresses mTORC1 activity, leading to the attenuation of aberrant SMC behavior, reduced inflammatory infiltration, and restoration of extracellular matrix integrity—collectively decelerating TAA advancement in our mouse model. …”
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Lung Matrix Metalloproteinase Activation following Partial Hepatic Ischemia/Reperfusion Injury in Rats by Giuseppina Palladini, Andrea Ferrigno, Vittoria Rizzo, Eleonora Tarantola, Vittorio Bertone, Isabel Freitas, Stefano Perlini, Plinio Richelmi, Mariapia Vairetti

“…The aim of the present study was to investigate whether acute liver I/R does affect the function and/or structure of remote organs such as lung, kidney, and heart via modulation of extracellular matrix remodelling. Methods. Male Sprague-Dawley rats were subjected to 30 min partial hepatic ischemia by clamping the hepatic artery and the portal vein. …”
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An ultrasound-driven PLGA/Zn-KNN hybrid piezoelectric scaffold with direct and immunoregulatory antibacterial activity for bone infection by Yuhao Zheng, Shu Wang, Wenhe Jin, Zhuoxuan Li, Guoju Yang, Xiaoxu Li, Ning Li, Yue Wang, Fan Sheng, Zhiming Song

“…Mechanistic investigation suggested that extracellular matrix ligands and complement and coagulation cascades may have a moderate effect on macrophage phagocytosis. …”
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Small spheroids for head and neck cartilage tissue engineering by Sven Reutter, Johann Kern, Yvonne Jakob, Nicole Rotter, David Gvaramia

“…Furthermore, we evaluated the influence of key chondrogenic cytokines, such as transforming growth factor (TGF)-β, connective tissue growth factor (CTGF), and insulin-like growth factor (IGF)-1, on spheroid morphology and the production of cartilage extracellular matrix (ECM) components. Spheroids expressing cartilage markers were formed with 2.5 × 104 cells in a commercially available chondrogenic differentiation medium on ULA plates but not in conventional micromass cultures. …”
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Positive Expression of IGFBP2 as a Novel Prognostic Biomarker for Patients with Lower-Grade Gliomas by Yanguo Li, Jiawei Fan, Zhuo Shen, Jun Zhang, Xuemei Yin, Fang Han, Wenfei Li

“…GSEA results revealed that five differential pathways involved in collagen binding, collagen-containing extracellular matrix, collagen metabolic process, collagen trimer, and extracellular structure organization were significantly enriched in patients with glioma with high IGFBP2 expression.Conclusion: Our study is the first to show that overexpression IGFBP2 could be an independent glioma biomarker. …”
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Glioblastoma-derived migrasomes promote migration and invasion by releasing PAK4 and LAMA4 by Zhe Huang, Ming Wang, Yitian Chen, Hua Tang, Kuo Tang, Mingkuan Zhao, Wei Yang, Zhengjun Zhou, Junjie Tian, Wei Xiang, Shenjie Li, Qinglian Luo, Luotong Liu, Yanru Zhao, Tao Li, Jie Zhou, Ligang Chen

“…We further confirmed that migrasomes are enriched in extracellular matrix (ECM)-related proteins such as p21-activating kinase 4 (PAK4) and laminin alpha 4 (LAMA4). …”
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Proteomic profiling of Labeo rohita intestine infected with Edwardsiella tarda: Unveiling the mucosal defense mechanisms, cell adhesion, and cytoskeletal pathways by Nevil Pinto, Mehar Un Nissa, Gowhar Iqbal, Yashwanth BS, Darshan P, Sanjeeva S, Mukunda Goswami

“…In E. tarda infected intestine, protein pathways were involved in defense response to the bacterium (muc-2), pyruvate metabolism (ldhb), integrin-mediated cell adhesion (ilk), actin filament bundle (lcp1), and collagen-containing extracellular matrix (nid2a). The control group mapped to pathways; innate immune system (mhc), carbon metabolism (rgn), and regulation of body fluid levels (fgg). …”
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Design of the Multi-Bioactive Graphene-Oxide/Gelatin/Alginate Scaffolds as Dual ECM-Mimetic and Specific Wound Healing Phase-Target Therapeutic Concept for Advanced Wound Healing by Marko Demenj, Martina Žabčić, Marija Vukomanović, Tatjana Ilić-Tomić, Dušan Milivojević, Simonida Tomić, Dubravka Živanović, Marija M. Babić Radić

“…<b>Objectives:</b> To develop and evaluate graphene oxide/gelatin/alginate scaffolds for advanced wound therapy capable of mimicking the native extracellular matrix (ECM) and bio-stimulating all specific phases of the wound healing process, from inflammation and proliferation to the remodeling of damaged skin tissue in three dimensions. …”
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Proteome‐Wide Mendelian Randomization Identifies Therapeutic Targets for Abdominal Aortic Aneurysm by Ka Zhang, Yuan Liu, Aiqin Mao, Changzhu Li, Li Geng, Hao Kan

“…Several causal proteins are involved in pathways regulating lipid metabolism, immune responses, and extracellular matrix organization. Nine proteins have already been targeted for drug development in diabetes and other cardiovascular diseases, presenting opportunities for repurposing as AAA therapeutic targets. …”
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Effects of linsitinib on M22 and IGF:1-treated 3D spheroids of human orbital fibroblasts by Fumihito Hikage, Megumi Suzuki, Tatsuya Sato, Araya Umetsu, Toshifumi Ogawa, Nami Nishikiori, Masato Furuhashi, Hiroshi Ohguro, Megumi Watanabe

“…For this purpose, we evaluated 1) cellular metabolic functions by using a seahorse bioanalyzer (2D), 2) physical properties including size and stiffness of 3D spheroids, and mRNA expression of several extracellular matrix (ECM) proteins, their modulators (CCL2 LOX, CTGF, MMPs), ACTA2 and inflammatory cytokines (IL1β, IL6). …”
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Pathogenesis of progressive scarring trachoma in Ethiopia and Tanzania and its implications for disease control: two cohort studies. by Matthew J Burton, Saul N Rajak, Victor H Hu, Athumani Ramadhani, Esmael Habtamu, Patrick Massae, Zerihun Tadesse, Kelly Callahan, Paul M Emerson, Peng T Khaw, David Jeffries, David C W Mabey, Robin L Bailey, Helen A Weiss, Martin J Holland

“…Chronic conjunctival inflammation appears to be central and is associated with enriched expression of pro-inflammatory factors and altered expression of extracellular matrix regulators. Host determinants of scarring progression appear more complex and subtle than the features of inflammation. …”
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The Differential Effects of Leukocyte-Containing and Pure Platelet-Rich Plasma on Nucleus Pulposus-Derived Mesenchymal Stem Cells: Implications for the Clinical Treatment of Interv... by Jun Jia, Shan-zheng Wang, Liang-yu Ma, Jia-bin Yu, Yu-dong Guo, Chen Wang

“…NPMSCs isolated from rabbit NP tissues were treated with L-PRP or P-PRP in vitro, and then cell proliferation and expression of stem cell markers, proinflammatory cytokines (TNF-α, IL-1β), production of ECM (extracellular matrix-related protein), and NF-κB p65 protein were validated by CCK-8 assay, real-time polymerase chain reaction, enzyme-linked immunosorbent assay, immunofluorescence, and western blot respectively. …”
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Obesity-induced mesenteric PVAT remodelling is sexually dimorphic, but not driven by ovarian hormones by Lisa Ivatt, Mhairi Paul, Allende Miguelez-Crespo, Patrick W. F. Hadoke, Matthew A. Bailey, Ruth A. Morgan, Mark Nixon

“…Results Transcriptomic analysis of mPVAT revealed sexually dimorphic responses to HFD, with upregulation of extracellular matrix (ECM) remodelling pathways in male but not female mice. …”
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Tumor-associated-fibrosis and active collagen-CD44 axis characterize a poor-prognosis subtype of gastric cancer and contribute to tumor immunosuppression by Yingqi Yang, Haohan Sun, Hongkai Yu, Luyao Wang, Chang Gao, Haokun Mei, Xiaomeng Jiang, Minghui Ji

“…Results A subtype of gastric cancer was identified with poor prognosis, characterized by higher malignancy, drug resistance, and poor immune infiltration, associated with elevated expression of genes related with Extracellular matrix (ECM). Single-cell transcriptome analysis showed active Collagen-CD44 signaling axis between cancer-associated fibroblasts (CAFs) and immune cells in gastric cancer, with ECM-related genes upregulated during tumor progression. …”
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Mouse Umbilical Cord Mesenchymal Stem Cell Paracrine Alleviates Renal Fibrosis in Diabetic Nephropathy by Reducing Myofibroblast Transdifferentiation and Cell Proliferation and Upr... by Hongde Li, Pengfei Rong, Xiaoqian Ma, Wei Nie, Yan Chen, Juan Zhang, Qiong Dong, Min Yang, Wei Wang

“…Next, mesangial cells were exposed to 5.6 mM glucose, 30 mM glucose, or mUC-MSC-conditioned medium, and then we performed western blotting, immunofluorescence, wound healing assay, and cell proliferation assay to measure extracellular matrix (ECM) proteins and matrix metalloproteinases (MMPs), myofibroblast transdifferentiation (MFT), and cell proliferation. …”
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Spatial transcriptomics unveils estrogen-modulated immune responses and structural alterations in the ectocervical mucosa of depot medroxyprogesterone acetate users by Vilde Kaldhusdal, Mathias Franzen Boger, Annelie Tjernlund, Adam D. Burgener, Frideborg Bradley, Julie Lajoie, Kenneth Omollo, Joshua Kimani, Keith Fowke, Paulo Czarnewski, Kristina Broliden

“…Downregulated genes represented spatially restricted disrupted epithelial barrier integrity and submucosal extracellular matrix dysfunction. The transcriptional profile was associated with markers of estrogen regulation. …”
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Proteomic signature of HIV-associated subclinical left atrial remodeling and incident heart failure by Tess E. Peterson, Virginia S. Hahn, Ruin Moaddel, Min Zhu, Sabina A. Haberlen, Frank J. Palella, Michael Plankey, Joel S. Bader, Joao A. C. Lima, Robert E. Gerszten, Jerome I. Rotter, Stephen S. Rich, Susan R. Heckbert, Gregory D. Kirk, Damani A. Piggott, Luigi Ferrucci, Joseph B. Margolick, Todd T. Brown, Katherine C. Wu, Wendy S. Post

“…This proteomic profile was statistically enriched for immune checkpoint proteins, tumor necrosis factor signaling, ephrin signaling, and extracellular matrix organization, identifying possible shared pathways in HIV and aging that may contribute to risk of heart failure.…”
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Loss of phosphatase and tensin homolog (PTEN) increases Lysyl oxidase-like 2 (LOXL2) expression enhancing the growth of fallopian tube epithelial cells as three-dimensional spheroi... by Angela Russo, Junlone Moy, Manead Khin, Timothy R. Dorsey, Alfredo Lopez Carrero, Joanna E. Burdette

“…In murine oviductal epithelial (MOE) cells (the equivalent of human FTE) loss of PTEN results in the upregulation of transcripts associated with the extracellular matrix, with a specific focus on the elevation of lysyl oxidase-like 2 (LOXL2). …”
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Inhibition of RAC1 activator DOCK2 ameliorates cholestatic liver injury via regulating macrophage polarisation and hepatic stellate cell activation by Jianli Qiu, Yitong Qu, Yinli Li, Cancan Li, Junling Wang, Lu Meng, Xiaojin Jing, Jiangping Fu, Yan Xu, Yuna Chai

“…At 14d post-BDL (chronic phase), DOCK2 knockdown suppressed hepatic stellate cell (HSC) activation and liver fibrosis as indicated by decreased α-SMA + HSCs and extracellular matrix deposition. In vitro experiments further demonstrated that DOCK2 knockdown suppressed M1 macrophage polarisation and HSC to myofibroblast transition, accompanied by inhibition of RAC1 activation. …”
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