Pharmacological approaches in drug-resistant pediatric epilepsies caused by pathogenic variants in potassium channel genes by Ilaria Filareto, Ilaria Mosca, Elena Freri, Francesca Ragona, Laura Canafoglia, Roberta Solazzi, Barbara Castellotti, Giuliana Messina, Cinzia Gellera, Maria Virginia Soldovieri, Paolo Ambrosino, Maurizio Taglialatela, Jacopo C. DiFrancesco, Tiziana Granata

“…We recruited six patients carrying different missense variants in four Kv channels (Kv7.2, Kv7.3, Kv3.1, and KNa1.1). In vitro experiments demonstrated that variants in Kv7 channels induced loss-of-function (LoF) effects, while those affecting Kv3.1 or KNa1.1 led to gain-of-function (GoF). …”
Get full text

CYP3A5 promotes glioblastoma stemness and chemoresistance through fine-tuning NAD+/NADH ratio by Wentao Hu, Xiaoteng Cui, Hongyu Liu, Ze Li, Xu Chen, Qixue Wang, Guolu Zhang, Er Wen, Jinxin Lan, Junyi Chen, Jialin Liu, Chunsheng Kang, Ling Chen

“…Loss-of-function or gain-of-function genetic experiments were performed to assess the impact of CYP3A5 on the self-renewal and chemoresistance of GSCs both in vitro and in vivo. …”
Get full text

Effects of the Oral Health Promotion Program on oral health and oral microbiota changes in diabetic elderly individuals: a quasi-experimental study by Fan Liu, Siping Song, Shuqi Huang, Jing He, Xin Ye, Liwei Hu, Xin Zeng, Sicheng Deng, Xiuying Hu

“…Moreover, microbial changes in the saliva microbiota community were also emphatically investigated. Methods A quasi-experiment was conducted in regionally representative communities to assess oral health and oral microbiota of the elderly diabetic participants. …”
Get full text

Feasibility of trancutaneous auricular vagus nerve stimulation in Black and Hispanic/Latino people with peripheral neuropathy by Marlon L. Wong, Eva Widerström-Noga, Jessica L. Bolanos, Gabriel Gonzalez, Frank J. Penedo, Peter J. Hosein, Melissa M. Tovin, Juan P. Gonzalez, Lisa M. McTeague

“…Exit interviews highlighted positive participant experiences and identified potential barriers, such as protocol length and distrust in medical research.ConclusionThe findings underscore the need for novel CIPN treatments and demonstrate the feasibility of conducting taVNS research in historically underrepresented populations. …”
Get full text

Assessing the role of genotype by environment interaction of winter wheat cultivars using envirotyping techniques in North China by Haiwang Yue, Yanbing Wang, Zhaoyang Chen, Jiashuai Zhu, Partha Pratim Behera, Pengcheng Liu, Haoxiang Yang, Jianwei Wei, Junzhou Bu, Xuwen Jiang, Xuwen Jiang, Wujun Ma

“…Accurate yield estimation depends on understanding the patterns of GEI in multi-environment trials (METs).MethodsFrom 2014 to 2018, continuous experiments were conducted in the Heilonggang region of the North China Plain (NCP), evaluating 71 winter wheat genotypes across 16 locations over five years. …”
Get full text

Therapeutic potential of rutin in premenstrual depression: evidence from in vivo and in vitro studies by Xiangjun Wang, Xiaowen Xia, Xianliang Song, Yi Zhou, Mingyu Ma, Yashuang Ren, Xitai Chen, Zenghui Xia, Yinghui Guo, Chunhong Song

“…The study of the efficacy and mechanism of action of rutin in PMDD-depressed subtype model rats plays an important role in the discovery of new drugs for the treatment of PMDD.MethodsBinding of rutin to gamma-aminobutyric acid type A receptors (GABAA receptors) was probed using molecular docking, microscale thermophoresis, radioactive receptor ligand binding assay and cell membrane clamp experiment. Behavioral tests in mice were performed to screen the optimal dose of rutin. …”
Get full text

Attitudes toward scale-up of an Intensive Combination Approach to Rollback the Epidemic in Nigerian adolescents (iCARE) intervention for youth in Nigeria: results of a mixed method... by Arthi Kozhumam, Revika Singh, Oche Agbaji, Adedotun Adetunji, Bopo Taiwo, Olayinka Omigbodun, Kehinde Kuti, Agatha David, Sulaimon Akanmu, Folashade Adekambi, Akinsegun Akinbami, Bibilola Oladeji, Babafemi Taiwo, Lisa M. Kuhns, Ogochukwu Okonkwor, Baiba Berzins, Amy K. Johnson, Titilope Badru, Patrick Janulis, Olubusuyi M. Adewumi, Marbella Cervantes, Olutosin Awolude, Robert Garofalo, Aima A. Ahonkhai, Lisa R. Hirschhorn

“…Facilitating factors and strategies across both interventions, included supportive institutional culture, ongoing supportive supervision, provision of a manual and training, relevant PN working experiences, communication methods designed to ensure anonymity of targeted youth (testing) or confidentiality (treatment), and access to cellular data and internet. …”
Get full text

Fusion of EEG and EMG signals for detecting pre-movement intention of sitting and standing in healthy individuals and patients with spinal cord injury by Chenyang Li, Chenyang Li, Yuchen Xu, Tao Feng, Tao Feng, Minmin Wang, Minmin Wang, Minmin Wang, Xiaomei Zhang, Li Zhang, Li Zhang, Ruidong Cheng, Weihai Chen, Weihai Chen, Weidong Chen, Weidong Chen, Shaomin Zhang, Shaomin Zhang, Shaomin Zhang, Shaomin Zhang

“…The robustness of the fusion method was further validated through a fatigue training experiment with healthy subjects. The fusion method achieved 92.87% accuracy during the post-fatigue stage, with no significant difference compared to the pre-fatigue stage (p > 0.05). …”
Get full text

Effects of dietary l-carnosine supplementation on the growth, intestinal microbiota, and serum metabolome of fattening lambs by Yaxuan Meng, Yaxuan Meng, Yaxuan Meng, Tingting Xian, Tingting Xian, Guolei Kang, Hongna Wang, Tao Feng, Tao Feng, Tao Feng

“…At the end of the 60-day experiment, all sheep were weighed, and fecal and blood samples were collected from 12 random sheep. …”
Get full text

Size-controllable synthesis of hydroxyapatite nanorods via fluorine modulation: applications in dental adhesives for enhanced enamel remineralization by Linzhi Meng, Mengmeng Shu, Peng Mei, Yakun Liang, Lunguo Xia

“…The remineralization was evaluated via SEM, energy dispersive X-ray spectroscopy (EDS), XRD, and micro-hardness through in vitro and in vivo experiments. Statistical analyses were performed using the Shapiro-Wilk test, Levene test, one-way ANOVA, Tukey HSD method, Welch test, Tamhane test, Fisher’s exact test and Kruskal-Wallis test (α = 0.05). …”
Get full text

Deficit irrigation enhances yield and water productivity of apples by inhibiting excessive vegetative growth and improving photosynthetic performance by Shenglin Wen, Ningbo Cui, Yaosheng Wang, Daozhi Gong, Liwen Xing, Zongjun Wu, Yixuan Zhang, Zhihui Wang

“…A two-year apple irrigation management experiment was carried out with 17 deficit drip irrigation (DDI) treatments, including a control treatment (CK, 100 % ETc) and 4 water deficit degree (W15 %, 85 % ETc; W30 %,70 % ETc; W45 %, 55 % ETc; W60 %, 40 % ETc) during four growth stages: bud burst to leafing stage (I), flowering to fruit set stage (II), fruit expansion stage (III), and fruit maturation stage (IV). …”
Get full text

Dietary Se-enrich Cardamine violifolia supplementation decreases lipid deposition and improves antioxidant status in the liver of aging laying hens by Mengke Yan, Xin Cong, Hui Wang, Kun Qin, Yuhui Tang, Xiao Xu, Dan Wang, Shuiyuan Cheng, Yulan Liu, Huiling Zhu

“…A total of 450 sixty-five-wk-old Roman laying hens were randomly divided into 5 treatments: a basal diet (without Se supplementation, CON) and basal diets supplemented with 0.3 mg/kg Se from sodium selenite (SS), 0.3 mg/kg Se from Se-enriched yeast (SEY), 0.3 mg/kg Se from SEC (SEC), or 0.3 mg/kg Se from SEC and 0.3 mg/kg Se from SEY (SEC + SEY). The experiment lasted for 8 wk. The results showed that dietary SEC + SEY supplementation decreased (P < 0.05) triglyceride (in the plasma and liver) and total cholesterol levels (in the plasma), and increased (P < 0.05) HDL-C concentration in plasma compared to CON diet. …”
Get full text

The Effect of Vascular Endothelial Growth Factor on Bone Marrow Mesenchymal Stem Cell Engraftment in Rat Fibrotic Liver upon Transplantation by Ke Yuan, Chunyou Lai, Lingling Wei, Tianhang Feng, Qinyan Yang, Tianying Zhang, Tao Lan, Yutong Yao, Guangming Xiang, Xiaolun Huang

“…According to existing related experiments and research reports, stem cell transplantation therapy has been shown to have a positive effect on the recovery of liver fibrosis/cirrhosis, but for some reason, this therapy still cannot be widely used in clinical work. …”
Get full text

Stimulation of Alpha1-Adrenergic Receptor Ameliorates Cellular Functions of Multiorgans beyond Vasomotion through PPARδ by Yong-Jik Lee, Hyun Soo Kim, Hong Seog Seo, Jin Oh Na, You-Na Jang, Yoon-Mi Han, Hyun-Min Kim

“…To investigate the effects and mechanism of AR stimulation on the physiology of the whole body, various in vitro and in vivo experiments were conducted using the AR agonist midodrine, 2-amino-N-[2-(2,5-dimethoxyphenyl)-2-hydroxy-ethyl]-acetamide. …”
Get full text

Comparison of two in vitro methods progressed in a computer-controlled simulated digestion system to determine amino acid digestibility of feed ingredients for yellow-feathered roo... by Yao Yu, Yuming Wang, Kaijing Ge, Jiang Chen, Jingjing Xie, Yi Zou, Songbai Liu, Huize Tan, Feng Zhao

“…This experiment compared amino acid (AA) digestibility assessed by 2 in vitro methods using a computer-controlled simulated digestion system and in vivo assay for corn, soybean meal, casein, corn gluten meal, cottonseed meal, rapeseed meal and a corn-soybean meal diet. …”
Get full text

Sinensetin attenuates hepatic ischemia-reperfusion injury through suppressing GRP78/CHOP-mediated endoplasmic reticulum (ER) stress in mice by Yihong Yang, Guanghua Xiong, Huangqi Shi, Yulin Peng, Jinghong Liu, Yaxuan Jiang, Min Lu, Hongbo Liu, Yong Liu

“…We also evaluated the protective effect of sinensetin in mice liver I/R injury through histopathological observation, enzyme activity, immunofluorescence, Western blot, molecular docking, and molecular pharmacology experiments.ResultsIn our study, we have successfully established the mouse HIRI injury model, and the liver function indicators such as ALT, AST and LDH were significantly increased in the HIRI model group, while SEN pretreatment could lead to a significant decrease in these enzymatic activities, especially perfusion at 6 h. …”
Get full text

Disulfidptosis-related gene signatures as prognostic biomarkers and predictors of immunotherapy response in HNSCC by Haotian Qin, Haotian Qin, Juan Xu, Yaohang Yue, Yaohang Yue, Meiling Chen, Zheng Zhang, Panpan Xu, Yan Zheng, Hui Zeng, Jian Weng, Jian Weng, Jun Yang, Fei Yu

“…The role of DSTN in HNSCC is further validated through gene knockout experiments.ResultsWe identified four valuable genes (SLC3A2, NUBPL, ACTB, DSTN) and constructed a prognostic model, along with identifying two DRG-related subtypes. …”
Get full text

Evaluating CNN Architectures for the Automated Detection and Grading of Modic Changes in MRI: A Comparative Study by Li‐peng Xing, Gang Liu, Hao‐chen Zhang, Lei Wang, Shan Zhu, Man Du La Hua Bao, Yan‐ni Wang, Chao Chen, Zhi Wang, Xin‐yu Liu, Shuai Zhang, Qiang Yang

“…In the AI‐assisted experiment, agreement between the junior doctor and the senior spine surgeon significantly improved from Cohen's kappa of 0.368–0.681. …”
Get full text

NALCN expression is down-regulated and associated with immune infiltration in gastric cancer by Xuewei Li, Xuewei Li, Na Wu, Chen Wang, Beibei Pei, Xiaoyan Ma, Jun Xie, Jun Xie, Wenhui Yang

“…Additionally, in vitro experiments were conducted to evaluate the impact of NALCN knockdown on T cells function in GC cell lines.ResultsRNA sequencing analysis revealed that NALCN expression was significantly downregulated in GC tissues. …”
Get full text

In vitro antidiabetic activity of facheiro (Pilosocereus pachycladus) mucilage and its effects on glycaemic, renal and hepatic parameters in obese rats by Fabrícia de Souza Ferreira, Hassler Clementino Cavalcante, Larissa Maria Gomes Dutra, Adriano Francisco Alves, Stephson Andriola Almeida Gonçalves, Tatiana Colombo Pimentel, Carlos Eduardo Barão, Marcos dos Santos Lima, Alana Natalícia Vasconcelos de Araújo, Januse Millia Dantas de Araújo, Kamila Sabino Batista, Jailane de Souza Aquino, Angela Maria Tribuzy de Magalhães Cordeiro

“…Then, the acute toxicity of mucilage was evaluated in Wistar rats for 14 days. After, an experiment to evaluate the effects of mucilage on obesity was conducted with 30 male Wistar rats randomised into three groups: healthy (HG); obese (OG); and obese treated with facheiro mucilage (FOG) by gavage (200 mg/kg). …”
Get full text