Balancing act: optimizing blue light for melanogenesis while minimizing cellular damage in primary human skin cells by Augustin C. Barolet, Augustin C. Barolet, Augustin C. Barolet, Augustin C. Barolet, Brice Magne, Brice Magne, Brice Magne, Karel Ferland, Karel Ferland, Karel Ferland, Natallia E. Uzunbajakava, Daniel Barolet, Daniel Barolet, Lucie Germain, Lucie Germain, Lucie Germain

“…In contrast, moderate blue light intensities (5–15 mW/cm2, 10–20 J/cm2) preserved cellular metabolic activity while stimulating α-MSH production, with an optimal balance achieved at 10 mW/cm2, 15 J/cm2 (1.14 ± 0.046 fold increase relative to sham on day 7). Co-culture experiments confirmed that irradiated keratinocytes enhanced melanogenesis in melanocytes via paracrine signaling, increasing the expression of Tyrosinase and Dopachrome Tautomerase (DCT). …”
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Patient centered medication treatment for opioid use disorder in rural Vermont: a qualitative study by Emily G. Hichborn, Owen B. Murray, Eilis I. Murphy, Tess E. Gallant, Sarah K. Moore, Bethany M. McLeman, John Saroyan, Anthony Folland, Megan Mitchell, Lisa A. Marsch

“…Methods Fifteen semi-structured telephone interviews were conducted from August through November of 2021 regarding patient experiences receiving MOUD in 13 Vermont Hub and Spoke clinics. …”
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Solving unknown primary cancer with earlier diagnosis - the SUPER-ED trial: study protocol for a stepped-wedge cluster randomised controlled trial to support earlier diagnosis for... by Anna Ugalde, Richard W. Tothill, Stephen Quinn, Hui-Li Wong, Owen Prall, Catherine Mitchell, Nilmini Wickramasinghe, Clare Fedele, Natalie Richards, Elizabeth Todio, Cindy Bryant, Louisa G. Collins, Sarah McLean, Hyun Soo Ko, Tim Akhurst, Christopher Steer, Bo Gao, Mark Wong, Chloe Georgiou, Narayan Karanth, Anna Kuchel, Louise Nott, Shamsudheen Padinharakam, Mark Shackleton, Ian M. Collins, Madhu Singh, Rachel Wong, Zee Wan Wong, Alexis Butler, Tharani Sivakumaran, Penelope Schofield, Linda Mileshkin

“…This trial aims to implement and evaluate an optimal model of care for people presenting with MUO to reduce time to diagnosis, improve patient experiences and reduce healthcare costs. Methods/design This is a pragmatic stepped-wedge cluster randomised trial comparing a control phase of standard practice with an intervention phase. …”
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Inhibition of S100A8/A9 ameliorates neuroinflammation by blocking NET formation following traumatic brain injury by Guihong Shi, Yiyao Cao, Jianye Xu, Bo Chen, Xu Zhang, Yanlin Zhu, Liang Liu, Xilei Liu, Luyuan Zhang, Yuan Zhou, Shenghui Li, Guili Yang, Xiao Liu, Fanglian Chen, Xin Chen, Jianning Zhang, Shu Zhang

“…Inhibition of S100A8/A9 effectively attenuated NET-mediated neuroinflammation; however, when PAD4 was overexpressed in the brain using adenovirus, leading to an increased formation of NET in the brain, the anti-inflammatory effects of S100A8/A9 inhibition were markedly diminished. Further experiments with PAD4 knockout mice confirmed that the reduction of NETs could substantially alleviate S100A8/A9-driven neuroinflammation. …”
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The potential use of brewers' spent grain-based substrates as horticultural bio-fertilizers by Angela Bianco, Angela Bianco, Sara Melito, Matteo Garau, Vittoria Giannini, Giacomo Zara, Giacomo Zara, Davide Assandri, Safa Oufensou, Safa Oufensou, Roberta Coronas, Niccolò Pampuro, Marilena Budroni, Marilena Budroni

“…Biochar (B), vermicompost (V), compost (CP), and compost enriched with a microbial consortium (CPE), each mixed at 2 and 4% with peat, were compared with two control treatments: 100% peat (C1) and peat with ternary chemical fertilizer (C2). The experiment was designed with six replicates, half of which were inoculated with Sclerotinia sclerotiorum mycelium during transplantation to assess the contribution of different substrates to pathogen resistance.ResultsOverall, it was found that the control treatment C2 was the most effective, yielding the greatest plant height at the seedbed stage and the best results across all parameters at harvest. …”
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Intravesical BCG in patients with non-muscle invasive bladder cancer induces trained immunity and decreases respiratory infections by J Alfred Witjes, Alina Vrieling, Mihai G Netea, Leo A B Joosten, Katja K H Aben, Sita H Vermeulen, Joost L Boormans, Antoine G van der Heijden, Boris Novakovic, Jelmer H van Puffelen, Liesbeth van Emst, Denise Kooper, Tahlita C M Zuiverloon, Ursula T H Oldenhof, Tessel E Galesloot, Lambertus A L M Kiemeney, Egbert Oosterwijk

“…An epigenomics approach combining chromatin immuno precipitation-sequencing and RNA-sequencing with in vitro trained immunity experiments identified enhanced inflammasome activity in BCG-treated individuals. …”
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Game-thinking; utilizing serious games and gamification in nursing education – a systematic review and meta-analysis by Mats Nylén-Eriksen, Marko Stojiljkovic, Daniela Lillekroken, Katrin Lindeflaten, Elisabeth Hessevaagbakke, Tone Nygaard Flølo, Olav Johannes Hovland, Ada Marie Svarstad Solberg, Sylvia Hansen, Ann Kristin Bjørnnes, Christine Tørris

“…As nursing education continues to adapt to the digital era, integration of game-thinking strategies could serve as a valuable method for creating engaging and effective learning experiences for nursing students.…”
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Dim blue light at night worsens high-fat diet-induced kidney damage via increasing corticosterone levels and modulating the expression of circadian clock genes by Wenji Ren, Zixu Wang, Yulan Dong, Jing Cao, Ting Gao, Qingyun Guo, Yaoxing Chen

“…Mechanistically, dBL increased plasma CORT levels as well as decreased renal GR α expression, and in vitro experiments showed that the circadian clock genes were altered by the addition of CORT and returned to normal levels after the addition of the GR inhibitor RU486. …”
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Evaluating the Performance of Artificial Intelligence-Based Large Language Models in Orthodontics—A Systematic Review and Meta-Analysis by Farraj Albalawi, Sanjeev B. Khanagar, Kiran Iyer, Nora Alhazmi, Afnan Alayyash, Anwar S. Alhazmi, Mohammed Awawdeh, Oinam Gokulchandra Singh

“…Conclusions: ChatGPT-4 has been extensively used for a variety of tasks and has demonstrated advanced and encouraging outcomes compared to other LLMs, and thus can be regarded as a valuable tool for enhancing educational and learning experiences. While LLMs can generate comprehensive responses, their reliability is compromised by the absence of peer-reviewed references, necessitating expert oversight in healthcare applications.…”
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Monitoring poultry social dynamics using colored tags: Avian visual perception, behavioral effects, and artificial intelligence precision by Florencia B. Rossi, Nicola Rossi, Gabriel Orso, Lucas Barberis, Raul H. Marin, Jackelyn M. Kembro

“…These tags can also accommodate sensors, such as accelerometers. In separate experiments, we aim to: (i) evaluate avian visual perception of the different colored tags; (ii) assess the potential impact of tag colors on social behavior; and (iii) test the ability of the YOLO model to accurately distinguish between different colored tags on Japanese quail in social group settings. …”
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Experimental infection of wild boars (Sus scrofa) with Rickettsia rickettsii and evaluation of the transmission potential to Amblyomma sculptum ticks by Lucianne Cardoso Neves, Lina de Campos Binder, Warley Vieira de Freitas Paula, Nicolas Jalowitzki de Lima, Ennya Rafaella Neves Cardoso, Rayane Almeida Santos, Raphaela Bueno Mendes Bittencourt, Gracielle Teles Pádua, Gabriel Cândido dos Santos, Mariana Avelar Tavares, Maria Carolina de Azevedo Serpa, Adriano Pinter, Artur Luiz de Almeida Felicio, Marcelo B. Labruna, Felipe da Silva Krawczak

“…IgG antibody titers were followed until the end of the experiment. Unfed nymphs and adults, molted from engorged larvae and nymphs that fed on wild boars, were used to infest susceptible guinea pigs and rabbits; some of these unfed ticks were tested by qPCR for rickettsial detection. …”
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Emergence of Self-Identity in Artificial Intelligence: A Mathematical Framework and Empirical Study with Generative Large Language Models by Minhyeok Lee

“…To validate this theoretical framework, we conducted empirical experiments using the Llama 3.2 1B model, employing low-rank adaptation (LoRA) for efficient fine-tuning. …”
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Terrain Irregularity Sensing by Evaluating Feet Coordinate Standard Deviation by Tomas Luneckas, Mindaugas Luneckas, Dainius Udris

“…We present simulation results that show that the <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>S</mi><mi>D</mi></mrow></semantics></math></inline-formula> value only depends on terrain roughness and is not influenced by large terrain slopes. Experiments were conducted with real robots while walking over obstacles with different gaits to validate the method. …”
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Effect of Kind and Dosage of Enrichment Materials on the Nutritional Quality of Rotifers Especially n3-HUFA by Muhammad Agus Suprayudi

“… This experiment was conducted to evaluate the quality of rotifer enriched with four  kinds of enrichement materials from the stand point of essensial fatty acids. …”
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Intestinal barrier function, caecal microbiota and growth performance of thermoneutral or heat stressed broiler chickens fed reduced crude protein diets supplemented with guanidino... by Reza Barekatain, Vivienne Inhuber, Nishchal Sharma, Tanya Nowland, Thi Thu Hao Van, Robert J. Moore, David Cadogan

“…The effectiveness of guanidinoacetic acid (GAA) in reduced protein (RP) diets on performance and gut health of broilers under heat stress is largely unknown. A 35-d experiment was conducted using four dietary treatments: a standard protein diet (SP, 22.1 and 20.7% CP in grower and finisher), a RP diet (20.1 and 18.7% in grower and finisher), a RP diet with 0.092% GAA per kg diet substituting 50% of supplemented arginine (GAA50) at one-to-one ratio and a RP diet with the same amount of GAA added on top (GAAtop). …”
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Breaking law of overburden rock and key mining technology for narrow coal pillar working face in isolated island by Du Feng, Li Zhenhua, Li Songtao, Li Xiaolei, Li Guodong, Fan Xuan, Ren Hao, Cao Zhengzheng

“…By using research methods such as on-site measurement, theoretical analysis, numerical simulation, and engineering experiments, starting from the stress state analysis and evolution law of coal seam mining, the dynamic evolution law of the overlying rock structure of sectional coal pillars has been mastered. …”
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Exploration of urease-aided calcium carbonate mineralization by enzyme analyses of Neobacillus mesonae strain NS-6 by Zhiwei Ma, Mengyao Chen, Juncheng Lu, Shichuang Liu, Yanling Ma

“…This study focused on the highly urease-producing bacterial Neobacillus mesonae NS-6 to explore the effects of specific nickel-ligand residues on the urease-aided CaCO3 mineralization process using molecular simulation predictions and targeted mutation experiments. The aim was to provide a molecular-level understanding of the interactive effects between urea and critical residues associated with the urease active center, as well as propose an effective modification strategy to enhance the application of UICP in future environmental areas.…”
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COVID-19 status and utilisation of essential maternal and child healthcare services during the pandemic in Ahmedabad, India by Sandul Yasobant, K Shruti Lekha, Ravina Tadvi, Bhavin Solanki, Walter Bruchhausen, Deepak Saxena

“…There were diverse experiences documented regarding access to essential maternal and child healthcare services during the pandemic. …”
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Effect of exposure time of pregnant females guppies, Poecilia reticulata Peters, in 17a-methyltestosterone solution on sex ratio of their offsprings by M. Zairin Junior, A. Yunianti, R.R.S.P.S. Dewi, Kusman Sumawidjaja

“… ABSTRACT This experiment was carried out to study the effect of female broodstocks immersion in 17amethyltestosterone (MT) solution on sex ratio of their offspring, Three-months old males and females were paired to mate in aquaria for four days.  …”
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Biodesulfurization of high-sulfur oil from the Karazhanbas field of Kazakhstan with deep eutectic solvents by A.O. Akimbek, G.A. Jamalova, A.K. Yernazarova, G.K. Kaiyrmanova, B.K. Yelikbayev, M.C. Pagano, A.G. Zazybin, Kh. S. Rafikova

“…As a result of a comparative analysis of microbial desulfurization processes, it was found that the highest biodesulfurization rate at the end of the experiment was recorded in cultures of Pseudomonas aeruginosa B-5807 (96.3 %), Bacillus thuringiensis SFN-3 (96.1 %), and Rhodococcus erythropolis AC 1039 (96 %).…”
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