Optimal protein requirement for rapid and coordinated test growth of juvenile sea urchin (Strongylocentrotus intermedius) by Panke Gong, Haijing Liu, Dan Gou, Weixiao Di, Huinan Zuo, Jun Ding, Yaqing Chang, Rantao Zuo

“…In this study, a 90-days satiety feeding experiment was performed to investigate the effects of different protein levels on the feed intake, somatic growth, and test characteristics of juvenile S. intermedius. …”
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MKRN1 promotes colorectal cancer metastasis by activating the TGF-β signalling pathway through SNIP1 protein degradation by Yi Zhang, Qin-shan Li, Hong-lin Liu, Hong-ting Tang, Han-lin Yang, Dao-qiu Wu, Yu-ying Huang, Li-cheng Li, Li-hong Liu, Meng-xing Li

“…A combination of MKRN1 quantitative proteomics, ubiquitination modification omics analysis, and a string of in vitro and in vivo experiments revealed the potential mechanisms by which MKRN1 regulates CRC metastasis. …”
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A preliminary exploration of establishing a mice model of hypoxic training by Minglu Li, Zhijie Chen, Ziyang He, Xinjuan Zhang, Yanqiu Liu, Hui Zhou, Hang Yang, Tao Liu, Xiaochuan Wang, Ran Zhang, Jiaxing Zhang

“…Then mice were removed from the chamber and tested at normoxic conditions weekly at the beginning of the experiment and the second, third, fourth, and sixth weeks. …”
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Differences in carbon and nitrogen metabolism of soft japonica rice in southern China during grain filling stage under different light and nitrogen fertilizer conditions and their... by Zhongtao Ma, Zhongtao Ma, Zhongtao Ma, Zhongtao Ma, Jiale Cao, Jiale Cao, Jiale Cao, Jiale Cao, Xi Chen, Xi Chen, Xi Chen, Xi Chen, Jianghui Yu, Jianghui Yu, Jianghui Yu, Jianghui Yu, Liu Guodong, Liu Guodong, Liu Guodong, Liu Guodong, Fangfu Xu, Fangfu Xu, Fangfu Xu, Fangfu Xu, Qun Hu, Qun Hu, Qun Hu, Qun Hu, Guangyan Li, Guangyan Li, Guangyan Li, Guangyan Li, Ying Zhu, Ying Zhu, Ying Zhu, Ying Zhu, Hongcheng Zhang, Hongcheng Zhang, Hongcheng Zhang, Hongcheng Zhang, Haiyan Wei, Haiyan Wei, Haiyan Wei, Haiyan Wei

“…To address this gap, field experiments were conducted under varying light intensities and nitrogen fertilizer levels to investigate the changes in carbon and nitrogen metabolism during grain filling, the eating quality of rice at maturity, and the relationship between them. …”
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miR-129-5p Promotes Osteogenic Differentiation of BMSCs and Bone Regeneration via Repressing Dkk3 by Changming Zhao, Yulin Gu, Yan Wang, Qiaozhen Qin, Ting Wang, Meng Huang, Heyang Zhang, Yannv Qu, Jingwen Zhang, Zhangzhen Du, Xiao-Xia Jiang, Lulu Xu

“…For the transfection experiments, lipofectamine 3000 was used to transfect pcDNA-Dkk3 into BMSCs to overexpress Dkk3. …”
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Study on the Key Influential Factors on Water Huff-n-Puff in Ultralow-Permeability Reservoir by Xiaoyan Wang, Kun Xie, Jie Zhang, Yong Zhang, Yang Zhang, Wei Wang, Xi Yan, Fengxiang Zhao

“…This manuscript studied the key influential factors of water huff-n-puff and related improving recovery methods using physical simulation experiments to put forward a beneficial condition for water huff-n-puff, combining with the field practice. …”
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Boosting electrocatalytic generation of FDCA and H2 from 2,5-furanedimethanol solution by carbonized wood supported Fe-CoP nanoleaves by Yuan Ma, Jiaojiao Miao, Yuanyuan Li, Xuehua Zhou, Lianbing Zhang, Jian Zhang, Guanglei Li, Yong Qin, Jie Gao

“…Herein, the effectiveness of Fe-CoP in catalyzing FDM oxidation was demonstrated by density functional theory (DFT) calculations and experiments, and a renewable carbonized porous wood decorated with Fe-doped CoP nanoleaves (Fe-CoP/CW) was constructed for electrocatalytic FDCA and hydrogen generation. …”
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Quantitative accuracy of preclinical in ovo PET/MRI: influence of attenuation and quantification methods by Theresa Balber, Katarína Benčurová, Manuela Mayrhofer, Joachim Friske, Martin Haas, Claudia Kuntner, Thomas H. Helbich, Marcus Hacker, Markus Mitterhauser, Ivo Rausch

“…This study aimed to investigate the influence of the different sources of attenuation on in ovo PET/MRI and assess the accuracy of MR-based AC for in ovo experiments. Method An in-house made chicken egg phantom was used to investigate the magnitude of self-attenuation and the influence of the MRI hardware on the PET signal. …”
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Mesenchymal stem cell-mediated adipogenic transformation: a key driver of oral squamous cell carcinoma progression by Yiting Shao, Yu Du, Zheng Chen, Lei Xiang, Shaoqin Tu, Yi Feng, Yuluan Hou, Xiaoxing Kou, Hong Ai

“…Subcutaneous xenograft experiments were conducted in BALB/c-nu mice to further investigate the effects in vivo. …”
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The use of patient-generated health data in the management of low anterior resection syndrome: a qualitative study by Olivia Monton, Olivia Monton, Allister Smith, Sarah Sabboobeh, Marie Demian, Julie Cornish, Steven D. Wexner, Peter Christensen, Peter Christensen, Amandeep Ghuman, Liliana G. Bordeianou, Liliana G. Bordeianou, Liliana G. Bordeianou, Celia Keane, Celia Keane, Syed Husain, Alessandra Gasior, Alessandra Gasior, Natalie Leon, Julie Savard, Lieba R. Savitt, Margit Majgaard, Gitte Kjær Sørensen, Melanie Mills, Fateme Rajabiyazdi, Marylise Boutros, Marylise Boutros

“…The objective of this study was to explore the perspectives and experiences of LARS experts regarding the use of PGHD in the management of LARS.MethodsWe utilized purposive snowball sampling to identify international LARS experts, including surgeons, nurses, and LARS researchers with knowledge and expertise in LARS. …”
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Temperature-dependent toxicity and mechanisms of florfenicol on the embryonic development of marine medaka (Oryzias melastigma) by Xingying Guo, Xu Gong, Jinhui Wang, Tan-Duc Nguyen, Syed Shabi Ul Hassan Kazmi, Jiezhang Mo, Feng Hua, Wenhua Liu, Zhen Wang

“…This study aimed to investigate the toxic effects and potential mechanisms of florfenicol (0.5, 10, 100, 500 and 1000 μg/L) on the embryonic development of marine medaka (Oryzias melastigma) through 21-d experiment under three temperature exposure scenarios (20, 25, and 30 °C). …”
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Wound-healing activity of solvent fractions and antiinflammatory activity of crude extract and solvent fractions of Acokanthera schimperi Schweinf (Apocynaceae) leaves in mice mode... by Workineh Woldeselassie Hammeso, Tesfaye Yimer, Getnet Tadege, Wakuma Wakene Jifar, Dejen Nureye

“…Purpose: Therefore, this experiment has been initiated to look into the solvent fractions' ability to heal wounds and the anti-inflammatory properties of the A. schimperi leaf 80 % methanol extract and its fractions in mice. …”
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Importin subunit beta‐1 mediates ERK5 nuclear translocation, and its inhibition synergizes with ERK5 kinase inhibitors in reducing cancer cell proliferation by Zoe Lombardi, Lucia Gardini, Anatolii V. Kashchuk, Alessio Menconi, Matteo Lulli, Ignazia Tusa, Alessandro Tubita, Luisa Maresca, Barbara Stecca, Marco Capitanio, Elisabetta Rovida

“…Both co‐immunoprecipitation experiments and proximity ligation assay provided evidence that ERK5 and importin β1 interact and that this interaction is further induced by EGF administration and prevented by ivermectin treatment. …”
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Multiresidue Pesticides Analysis of Vegetables in Vietnam by Ultrahigh-Performance Liquid Chromatography in Combination with High-Resolution Mass Spectrometry (UPLC-Orbitrap MS) by Nam Vu-Duc, Trung Nguyen-Quang, Thuy Le-Minh, Xuyen Nguyen-Thi, Tri Manh Tran, Hai Anh Vu, Lan-Anh Nguyen, Tien Doan-Duy, Bui Van Hoi, Cam-Tu Vu, Dung Le-Van, Lan-Anh Phung-Thi, Hong-An Vu-Thi, Dinh Binh Chu

“…The matrix effect, extraction recovery, and overall recovery were fully investigated by spiking experiments. Experimental results demonstrated that the ionization suppression or enhancement was the main contribution on the overall recoveries of target analytes. …”
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Proteogenomic annotation of T6SS components identified in Bacteroides fragilis secretome by Natalya B. Zakharzhevskaya, Olga Yu Shagaleeva, Daria A. Kashatnikova, Daria A. Kashatnikova, Anton O. Goncharov, Anton O. Goncharov, Daria V. Evsyutina, Dmitry A. Kardonsky, Elizaveta A. Vorobeva, Artemiy S. Silantiev, Viktoria D. Kazakova, Irina V. Kolesnikova, Ivan O. Butenko, Anna A. Vanyushkina, Svetlana V. Smirnova, Andrei V. Chaplin, Andrei V. Chaplin, Boris A. Efimov, Boris A. Efimov

“…IntroductionBacteroides fragilis (Bf)’s T6SS locus has been characterized and shown to have functional activity in competition experiments. It has been demonstrated that symbiont nontoxigenic Bf strains have a more effective “weapon” effect on pathogenic Bf, which is realized through the activity of effector-immune (E-I) protein pairs. …”
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Patient preferences for anti-hyperglycaemic medication for type 2 diabetes mellitus in China: findings from a national survey by Jing Liu, Lei Si, Yingyao Chen, Yanfeng Ren, Liu Liu, Shimeng Liu, Qi Pan, Xiong Ke, Shiyi Bao, Fuming Li, Yijiang Yu, Yan Wei

“…Objective This study aimed to investigate the preferences regarding risks, benefits and other treatment attributes of patients with type 2 diabetes mellitus (T2DM) in China when selecting a second-line anti-hyperglycaemic medicine.Methods A discrete choice experiment with hypothetical anti-hyperglycaemic medication profiles was performed using a face-to-face survey administered to patients with T2DM. …”
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Organic Fertilization Leads to N Limitation Rather than P Limitation in Both Vegetable Soils by Qingshan Li, Mengqian Xu, Lingying Xu, Xingwang Wu, Yuqin Zhang, Jia Xin, Yazhen Shen, Jichao Gao

“…The distribution of soil nutrients and enzyme activities was analyzed under controlled conditions during a pot experiment. The addition of organic amendments, regardless of their source, significantly altered the microbial resource allocation, reducing the carbon limitation while increasing the nitrogen demand. …”
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Exploring the shared gene signatures and mechanism among three autoimmune diseases by bulk RNA sequencing integrated with single-cell RNA sequencing analysis by Xiaofang Liu, Bin Li, Yuxi Lin, Xueying Ma, Yingying Liu, Lili Ma, Xiaomeng Ma, Xia Wang, Nanjing Li, Xiaoyun Liu, Xiaohong Chen

“…Finally, the biomarkers’ expression was validated in human and mice experiments.ResultsSeveral shared genes among MS, SLE, and RA were identified, which play crucial roles in immune responses and inflammation regulation. …”
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Characterization of three novel Helicobacter species infecting stomachs of dogs and cats: Helicobacter gastrocanis sp. nov., Helicobacter gastrofelis sp. nov., and Helicobacter fel... by Emiko Rimbara, Sae Aoki, Masato Suzuki, Hirotaka Kobayashi, Taisuke Nakagawa, Yuko Goto-Koshino, Sachiyo Nomura, Sachiyo Nomura, Wan-Ying Du, Hidenori Matsui, Shigetarou Mori, Keigo Shibayama, Tsuyoshi Kenri, Koichi Ohno

“…Additionally, 10.6% of dogs and 20.8% of cats were infected with H. felistomachi. Animal experiments have confirmed that these three novel species elicit gastric inflammatory responses. …”
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Combining single-cell analysis and molecular docking techniques to construct a prognostic model for colon adenocarcinoma and uncovering inhibin subunit βb as a novel therapeutic ta... by Qinqing Wu, Qinqing Wu, Lu Ye, Yuwei Wu, Mengyu Zhao, Mengyu Zhao, Jiaxin Lu, Yanping Yu, Yanping Yu, Yixiao Niu, Yixiao Niu, Luxiao Zhang, Peijun Zuo, Peijun Zuo

“…Furthermore, in vitro experiments with INHBB showed that downregulation of its expression in COAD cell lines significantly reduced cellular viability and migration capacity.ConclusionIn summary, our research, based on the expression characteristics of ARGs, provides new insights into the precise classification, prognosis assessment, and identification of potential therapeutic targets in COAD. …”
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