Sex differences in the neuroinflammatory signaling pathway: effect of miRNAs on fatty acid synthesis in microglia by Haolin Zheng, Akiko Mizokami, Sergio Romera-Giner, Jaime Llera-Oyola, Yosuke Yamawaki, Tomomi Sano, Eijiro Jimi, Francisco García-García, Takashi Kanematsu

“…The mouse microglial cell line MG6 was used for in vitro experiments. Following testosterone treatment, miRNA, gene, and protein expression levels were investigated. …”
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Boanmycin overcomes bortezomib resistance by inducing DNA damage and endoplasmic reticulum functional impairment in multiple myeloma by Jin-Xing Wang, Ling Zhang, Peng-Wei Zhang, Luo-Wei Yuan, Jian Jiang, Xiao-Hui Cheng, Wei Zhu, Yong Lei, Fa-Qing Tian

“…Colony formation ability was evaluated, and cell apoptosis and the cell cycle were detected through flow cytometry. Xenograft experiments were conducted to evaluate the growth of MM cells in vivo. …”
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Reinvestigation into the role of lipopolysaccharide Glycosyltransferases in Helicobacter pylori protein glycosylation by Hong Li, Xiaoqiong Tang, Tiandi Yang, Tingting Liao, Aleksandra W. Debowski, Tiankuo Yang, Yalin Shen, Hans-Olof Nilsson, Stuart M. Haslam, Barbara Mulloy, Anne Dell, Keith A. Stubbs, Wolfgang Fischer, Rainer Haas, Hong Tang, Barry J. Marshall, Mohammed Benghezal

“…Moreover, using LPS-protein reconstitution experiments, we demonstrate that the presence of O-antigen containing full-length LPS interferes with the electrophoretic mobility of H. pylori RecA and many other proteins including AlpA/B on SDS-PAGE. …”
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Deep application of controlled-release urea increases the yield and saponin content of Panax notoginseng by regulating soil nitrate distribution by Yun-xia Su, Yun-xia Su, Yun-xia Su, Ping Zhao, Li-jie Jia, Li-jie Jia, Li-jie Jia, Yuan-feng Cao, Guan-ze Liu, Guan-ze Liu, Jun-wen Chen, Jun-wen Chen, Sheng-chao Yang, Sheng-chao Yang, Yan-li Zhou, Guang-qiang Long, Guang-qiang Long, Guang-qiang Long

“…However, its effects on P. notoginseng yield and quality, a medicinal plant with a prolonged nutrient acquisition duration, remain unclear.MethodsIn this study, we conducted a two-year field plot experiment to investigate the effect of CRU on P. notoginseng with three placement depths (0, 6, and 12 cm denoted as R0, R6, and R12, respectively) at an application dosage of 250 kg N ha-1 with biochar addition (R6B) and 20% N reduction (R6R) based on the R6, with conventional fertilization (250 kg N ha-1, common urea) serving as the control (CK).ResultsOur results indicated that yields increased by 27.1–37.6% with R0, R6, R12, and R6B, while remaining stable with R6R compared to CK. …”
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Dental Pulp Mesenchymal Stem Cells Attenuate Limb Ischemia via Promoting Capillary Proliferation and Collateral Development in a Preclinical Model by Youfeng Li, Yuning Zhang, Hua Wang, Chengfeng Sun, Dongmei Liu, Hongying Liu, Jia He, Furong Chen, Weiting Wang, Xi Jiang, Chu-tse Wu, Yuefeng Yang

“…Although neovascularization was induced in the ischemic limb after ligation, MSCs, especially DP-MSCs, significantly enhanced the angiogenesis. In vitro experiments showed that serum deprivation improved the expression of angiogenic factors, growth factors, and chemokines in DP-MSCs and UC-MSCs, but not in BM-MSCs and AD-MSCs. …”
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Superior biomechanical stability of pedicle screws compared to lateral mass screws: recommendations for bicortical positioning and enhancing bone contact in geriatric C1 vertebrae by Leon-Gordian Leonhardt, Leonie Rörup, Anna Lena Kammal, Michael Hahn, Marc Dreimann, Benjamin Ondruschka, Felix Nikolai von Brackel, Tim Rolvien, Lennart Viezens, Simon von Kroge

“…Simultaneously, a higher number of cycles and a higher maximum force was needed to loosen PS compared to LMS, while the loose by torque at the experiment end was still greater in PS. Differences between mono- and bicortical positioning of PS and LMS have only been observed in the initial stiffness of screws. …”
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Optimal radiation dose to induce an abscopal effect by combining carbon-ion radiotherapy and anti-CTLA4 antibody by Liqiu Ma, Yang Li, Yoshimitsu Sakamoto, Lin Xie, Saaya Suzuki, Yukari Yoshida, Li Sui, Gang Guo, Jialing Wen, Wangcai Ren, Kazuhiro Kakimi, Kensuke Osada, Akihisa Takahashi, Takashi Shimokawa

“…Materials and methods: Murine cancer cells (colon carcinoma Colon-26 cells for experiments and osteosarcoma LM8 cells for verification) were grafted into both sides of the hind legs of syngeneic mice. …”
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Influence of lemongrass and oregano essential oils and their combination on in vitro ruminal fermentation and greenhouse gas emissions in total mixed ration for dairy cows by Sara Glorio Patrucco, Alessandro Lotto, Martina Dorigo, Rita Fornaciari, Antonio Sagliano, Nicola Martinelli, Alessandra Cosani, Khalil Abid, Salvatore Barbera, Hatsumi Kaihara, Sonia Tassone

“…Among EOs, lemongrass (Cymbopogon winterianus) and oregano (Origanum vulgare) EOs (LEO and OEO) have attracted attention as modulators of ruminal fermentations, but their role needs to be clarified. The experiment was designed using a randomised setup to assess the effects of LEO and OEO on in vitro ruminal fermentation and GHGs, using total mixed ration (TMR) as substrate (incubation time 24h). …”
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The Isolation of Lead-Tolerant PGPR from Red Clover Soil and Its Role in Promoting the Growth of Alfalfa by Wanting Nie, Yuchen Wu, Jingwen Jiang, Zicheng Wang, Meiqi Mu, Siwen Zhao, Minghao Yang, Xi Long, Xiujie Yin, Xiaohua Teng

“…(strain Y23) exhibited a strong growth-promoting ability in alfalfa, and there was no antagonistic reaction among the three strains, enabling their coexistence. The pot experiment manifested that strains Y2, Y22, Y23, and YH (a mixture of Y2, Y22, and Y23) could increase the plant height, root length, fresh and dry weight above ground, and fresh and dry weight below ground of alfalfa. …”
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Shifts in the rhizosphere microbiome and exudation profile of avocado (Persea americana Mill.) during infection by Phytophthora cinnamomi and in presence of a biocontrol bacterial... by Frédérique Reverchon, Mariel García-Meléndez, Edgar Guevara-Avendaño, Oscar Mora-Chávez, Itzel A. Solís-García, Wesley Dáttilo, José A. Guererro-Analco, Alfonso Méndez-Bravo, Juan L. Monribot-Villanueva, Violeta Patiño-Conde, Fernando Pineda-García, Eria A. Rebollar

“…Dataset presentation Here, we present a novel dataset collected from a time-course experiment with four treatments: (1) control trees; (2) trees infected with P. cinnamomi; (3) trees inoculated with the BCA; (4) trees infected with P. cinnamomi and inoculated with the BCA. …”
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miR-223 and Chromogranin A Affect Inflammatory Immune Cell Activation in Liver Metastasis of Neuroendocrine Neoplasms by Lukas Geisler, Katharina Detjen, Teresa Hellberg, Marlene Kohlhepp, Carsten Grötzinger, Jana Knorr, Ines Eichhorn, Raphael Mohr, Theresa Holtmann, Bertram Wiedenmann, Frank Tacke, Christoph Roderburg, Alexander Wree

“…Subsequently, in vitro transfections using the well-established human pancreatic NEN cell line (BON), and co-culture experiments with primary macrophages and neutrophils were performed. …”
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Optimizing Flammulina velutipes residue use: impact on health metrics and performance in three-yellow chickens by Yuanxiao Li, Yuanxiao Li, Dan Liu, Wang Li, Wang Li, Longmei Zhao, Pinghua Cao, Yanbo Ma, Yanbo Ma, Ke Ding, Cai Zhang, Cai Zhang, Ning Liu

“…The control group (CON) was fed a basal diet, while the remaining three groups were supplemented with FVR in the basal diet, adding 2%, 4% and 6% of the basal diet, respectively. The experiment lasted for 48 days. Blood samples were collected from the jugular vein on days 28 and 48 to determine serum biochemical indices. …”
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Exploring the role of ELOVLs family in lung adenocarcinoma based on bioinformatic analysis and experimental validation by Zihan Wang, Wenjing Cui, Long Liang, Jingge Qu, Yuqiang Pei, Danyang Li, Ying Luo, Yue Zhang, Yifan Qiu, Yongchang Sun

“…Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis, cell counting kit-8 (CCK8), colony formation, wound healing, transwell migration assays and fatty acid metabolism detection were employed to confirm the significant role of ELOVL6 in vitro experiment. Results Our results revealed that mRNA expression levels of ELOVL2, ELOVL4 and ELOVL6 and protein expression levels of ELOVL5 and ELOVL6 were elevated in LUAD tissues compared to normal subjects. …”
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Reconstruction for Time-Domain In Vivo EPR 3D Multigradient Oximetric Imaging—A Parallel Processing Perspective by Christopher D. Dharmaraj, Kishan Thadikonda, Anthony R. Fletcher, Phuc N. Doan, Nallathamby Devasahayam, Shingo Matsumoto, Calvin A. Johnson, John A. Cook, James B. Mitchell, Sankaran Subramanian, Murali C. Krishna

“…However, this involves dealing with gigabytes of data for each 3D oximetric imaging experiment involving digital band pass filtering and background noise subtraction, followed by 3D Fourier reconstruction. …”
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DNMT1-driven methylation of RORA facilitates esophageal squamous cell carcinoma progression under hypoxia through SLC2A3 by Wenjian Yao, Linlin Shang, Yinghao Wang, Lei Xu, Yu Bai, Mingyu Feng, Xiangbo Jia, Sen Wu

“…Furthermore, rescue experiments revealed that RORA-mediated suppressive effects on ESCC cell migration and invasion were largely based on its negative regulation of SLC2A3 and glycolysis. …”
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Induction of SUSD2 by STAT3 Activation Is Associated with Tumor Recurrence in HER2-Positive Breast Cancer by Yisun Jeong, Hyungjoo Kim, Daeun You, Soo Youn Cho, Sun Young Yoon, Seok Won Kim, Seok Jin Nam, Jeong Eon Lee, Sangmin Kim

“…Through kinase array experiments, we found that SUSD2 expression was modulated downstream of STAT3-dependent signaling in breast cancer cells overexpressing HER2. …”
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When is “convenient time” for residents?: a trial of Resident Study Log as asynchronous learning tool for residents by Tadayuki Hashimoto, Shoko Ariyoshi, Taira Ariyoshi, Ryosuke Horitani, Mohammad Adrian Hasdianda, Kanapa Kornsawad, Shunsuke Kosugi, Makoto Kikukawa, Tomio Suzuki

“…This tool facilitated residents to share daily reflections on their learning experiences, allowing supervising physicians outside of their regular clinical teams to provide feedback. …”
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The perceived workload of first-line healthcare professionals during neonatal resuscitation by Hai-Bo Huang, Kui Sang, Ming Zhou, Lin Yi, Jiang-Qin Liu, Chuan-Zhong Yang, Brenda H.Y. Law, Georg M. Schmölzer, Po-Yin Cheung

“…Little is known regarding the perceived workload and associated factors among healthcare professionals including medical doctors (MDs) and nurses/midwives who have differences in training and experiences. We aimed to characterize and compare the perceived workload between MDs and nurses/midwives who provided neonatal resuscitation. …”
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Imaging flow cytometry-based cellular screening elucidates pathophysiology in individuals with Variants of Uncertain Significance by Irena Josephina Johanna Muffels, Hans R. Waterham, Giuseppina D’Alessandro, Guido Zagnoli-Vieira, Michael Sacher, Dirk J. Lefeber, Celine Van der Vinne, Chaim M. Roifman, Koen L. I. Gassen, Holger Rehmann, Desiree Y. Van Haaften-Visser, Edward S. S. Nieuwenhuis, Stephen P. Jackson, Sabine A. Fuchs, Femke Wijk, Peter van Hasselt

“…Conclusions Our high-throughput IFC-based platform simplifies the process of identifying VUS pathogenicity through six assays and allows for the recognition of useful pathophysiological markers that structure follow-up experiments, thereby representing a novel valuable tool for precise functional diagnostics in genomics.…”
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Subsurface drip irrigation mitigated greenhouse gas emission and improved root growth and yield in apple in semi-arid region by Jiabin Liu, Xiaodong Gao, Juanjuan Song, Mingyi Wen, Jianjun Wang, Yaohui Cai, Xining Zhao

“…We conducted a two-year field experiment to research the effects of surface drip irrigation (DI), subsurface drip irrigation (SDI), and no irrigation (CK) on soil GHG emissions (CH4, CO2, N2O), as well as the regulatory mechanisms of yield and root growth (root length density, diameter) in apple orchards. …”
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