Usp11 maintained the survival of marginal zone B cells under ionizing radiation by deubiquitinating DLL1 and JAG2 by Jiaqi Sheng, Depei Wu, Jingzhe Shang, Xiaodan Fu, He Gao, Jianjie Rong, Jun Wang, Jiancheng Hu, Xiaofei Qi

“…Conducted Co-IP and ubiquitination experiments for mechanism elucidation. Quantified IgM and IgG using ELISA and FC. …”
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Exploration of the feasibility of clinical application of phage treatment for multidrug-resistant Serratia marcescens-induced pulmonary infection by Xiangke Duan, Wenfeng Liu, Yanyu Xiao, Man Rao, Liyin Ji, Xiaofu Wan, Shuhong Han, Zixun Lin, Haichen Liu, Peifen Chen, Kun Qiao, Mingbin Zheng, Jiayin Shen, Yang Zhou, Tetsuya Asakawa, Minfeng Xiao, Hongzhou Lu

“…This study was conducted to explore the feasibility of phage application in treating refractory S. marcescens infection by collaborating with a 59-year-old male patient with a pulmonary infection of multidrug-resistant S. marcescens. Our experiments included three domains: i) selection of the appropriate phage, ii) verification of the efficacy and safety of the selected phage, iii) confirmation of phage-bacteria interactions. …”
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Serum-Free Culture System for Spontaneous Human Mesenchymal Stem Cell Spheroid Formation by Guoyi Dong, Shengpeng Wang, Yuping Ge, Qiuting Deng, Qi Cao, Quanlei Wang, Zhouchun Shang, Wenjie OuYang, Jing Li, Chao Liu, Jie Tang, Weihua Zhao, Ying Gu

“…Through component deletion screening and complementation experiments, the knockout serum replacement (KSR) was identified as necessary and sufficient for hMSC spheroid formation. …”
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Verbal fluency and semantic association deficits in children with in birth nonprogressive neuromuscular diseases by Maria Koriakina, Maria Koriakina, Olga E. Agranovich, Ioannis Ntoumanis, Maxim Ulanov, Isak B. Blank, Anna Shestakova, Evgeny Blagovechtchenski

“…IntroductionThe relationship between motor and cognitive skills is a pivotal issue in neuroscience, with embodied cognition theory asserting that bodily actions and experiences play a vital role in cognitive processing. …”
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Synergistic Effect of Sugarcane Bagasse and Zinc Oxide Nanoparticles on Eco-Remediation of Cadmium-Contaminated Saline Soils in Wheat Cultivation by Emad M. Hafez, Khadiga Alharbi, Hany S. Gharib, Alaa El-Dein Omara, Essam Elatafi, Maha M. Hamada, Emadelden Rashwan, Tarek Alshaal

“…This study explores the combined application of sugarcane bagasse (SCB) and zinc oxide nanoparticles (ZnO NPs) to mitigate the toxic effects of Cd and salinity in wheat plants. Field experiments conducted in Cd-contaminated saline soils revealed that the application of SCB (0, 5, and 10 t ha⁻¹) and ZnO NPs (0, 12.5, and 25 mg L⁻¹) significantly improved key soil physicochemical properties, including soil pH, electrical conductivity (EC), and exchangeable sodium percentage (ESP). …”
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Mesenchymal Stromal Cells Accelerate Epithelial Tight Junction Assembly via the AMP-Activated Protein Kinase Pathway, Independently of Liver Kinase B1 by P. Rowart, P. Erpicum, J.-M. Krzesinski, M. Sebbagh, F. Jouret

“…TJ assembly was measured by assessing ZO-1 relocation to cell-cell contacts. Experiments were conducted using MDCK stably expressing short-hairpin-RNA (shRNA) against LKB1 or luciferase (LUC, as controls). …”
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The Origin of the Matter Matters: The Influence of Terrestrial Inputs on Coastal Benthic Microeukaryote Communities Revealed by eDNA by Mathisse Meyneng, Raffaele Siano, Naïna Mouras, Dominique Ansquer, Christine Laporte‐Magoni, Florence Antypas, Thomas Haize, Hugues Lemonnier

“…We hypothesized that the association between microeukaryotes and metallic compositions is linked to selection processes, given the resistance of some microeukaryotes to some high metallic concentrations. In vitro experiments are needed to confirm this hypothesis. Our results emphasized the role of terrestrial inputs in shaping estuarine diversity and the need to consider the entire land–sea continuum for studying these ecosystems.…”
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A strain of Talaromyces assiutensis provides multiple protection effects against insect pests and a fungal pathogen after endophytic settlement in soybean plants by Li Sui, Yang Lu, Ke Cheng, Yifan Tian, Zhiming Liu, Zhao Xie, Zhengkun Zhang, Qiyun Li

“…TaS1GZL-1 colonization upregulated the expression levels of 12 genes related to defense pathways in soybean lateral roots, thereby inducing plant tolerance against phytopathogen infection, as well as disrupting the feeding selectivity of S. litura larvae. Semi-field experiments verified that TaS1GZL-1 had practical control effects on S. litura larvae and sclerotinia disease. …”
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AKT1-Mediated NOTCH1 phosphorylation promotes gastric cancer progression via targeted regulation of IRS-1 transcription by Lingshan Zhou, Yuan Yang, Qian Qiao, Yingying Mi, Yuling Gan, Ya Zheng, Yuping Wang, Min Liu, Yongning Zhou

“…Overexpression of Notch1-IC was shown to promote the proliferation, invasion, and metastasis of GC cells, while knockdown of IRS-1 partially inhibited the aforementioned effects induced by Notch1-IC overexpression. Further experiments in vitro and vivo confirmed that AKT1-induced Notch1 phosphorylation can regulate the expression of IRS-1 and promote the malignant behavior of GC, including proliferation, invasion, metastasis, and EMT, with knockdown of IRS-1 partially reversing these effects. …”
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Development of functional beverage with rice bran protein (Hom mali 105) by Saveboworn Wanticha, Rittisak Sriwiang, Schoenlechner Regine, Wangtueai Sutee, Charoen Ratchanee

“…The development of ready-to-drink fruit juice offers consumers convenience and creates new experiences from functional beverages that were available in the market. …”
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Identification and Analysis of Components in Yizhi Granule and Cynomolgus Monkey Plasma after Oral Administration by UPLC/ESI-Q-TOF MS and Their Protective Effects on PC12 Cells by Erwei Hao, Jianfeng Qin, Wei Wei, Jianhua Miao, Yan Xie, Xianglong Pan, Hangxuan Wu, Jinling Xie, Xiaosu Fan, Zhengcai Du, Xiaotao Hou, Jiagang Deng

“…It improves memory barriers in rat experiments. Here, we describe the first fast and sensitive ultraperformance liquid chromatography/electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF MS) method for analyzing YZG in plasma. …”
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Biodegradation of Azo and disperse dyes by Trametes versicolor: Process optimization and MnP enzyme dynamics by Isma Fayyaz, Salina Saddick, Raja Tahir Mahmood, Muhammad Javaid Asad, Muhammad Altaf Hussain, Jiandong Hu, Muhammad Awais, M. Ijaz Khan, Shaxnoza Saydaxmetova

“…The effect of manganese peroxidase (MnP) on the biodegradation was also monitored and it was partially purified as well as characterized. The screening experiments showed that this fungus has higher biodegradation potential for direct dyes (pink B = 58.9 % & yellow BG= 54.6 %) compared to disperse dyes (redS3B= 43.12 % & yellow SRLB= 27 %). …”
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A cohort-based multi-omics identifies nuclear translocation of eIF5B /PD-L1/CD44 complex as the target to overcome Osimertinib resistance of ARID1A-deficient lung adenocarcinoma by Dantong Sun, Helei Hou, Feiyue Feng, Weizheng Wu, Jingyu Tan, Tongji Xie, Jiayu Liu, Jinsong Wang, Haili Qian, Junling Li, Puyuan Xing

“…To validate the findings, in vitro and in vivo molecular experiments were conducted. Results We found that the ARID1A deficiency results in resistance to Osimertinib by hindering programmed cell death through the EZH2/PTEN/E2F1 axis. …”
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Ethanol Extracts of Solanum lyratum Thunb Regulate Ovarian Cancer Cell Proliferation, Apoptosis, and Epithelial-to-Mesenchymal Transition (EMT) via the ROS-Mediated p53 Pathway by Chen Zhang, Zheming Li, Jie Wang, Xuelu Jiang, Mengting Xia, Jianfen Wang, Shenyi Lu, Shouye Li, Hanmei Wang

“…Lactate dehydrogenase (LDH) and MTT assays indicated that SLT concentrations of 0.25 and 0.5 μg/mL were not cytotoxic and had significant inhibitory effects on the cell viabilities of A2780 and SKOV3 cells, hence were used for subsequent experiments. Flow cytometric and western blot analysis revealed that SLT effectively suppressed ovarian cancer cell proliferation via inducing cell cycle arrest and increasing apoptosis. …”
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Assessing the suitability of sites near Pine Island Glacier for subglacial bedrock drilling aimed at detecting Holocene retreat–readvance by J. S. Johnson, J. Woodward, I. Nesbitt, K. Winter, S. Campbell, K. A. Nichols, R. A. Venturelli, S. Braddock, B. M. Goehring, B. Hall, D. H. Rood, G. Balco, G. Balco

“…Furthermore, the ridge is strewn with glacial erratics at all elevations, providing valuable constraints on its early Holocene deglacial history. Based on our experiences during this study, we conclude with a series of recommendations for assessing site suitability for future bedrock drilling campaigns. …”
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CD-1 mice females recognize male reproductive success via volatile organic compounds in urine by A. S. Khotskina, E. L. Zavjalov, E. P. Shnayder, L. A. Gerlinskaya, S. O. Maslennikova, D. V. Petrovskii, M. N. Baldin, A. L. Makas, V. M. Gruznov, M. L. Troshkov, M. P. Moshkin

“…The aim of our study was to find out if male mice could demonstrate their reproductive efficiency through urine volatile organic compounds. The experiment implies cohabiting one male with two mature females for 6 days. …”
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Composting of chicken manure with a mixture of sawdust and wood shavings under forced aeration in a closed reactor system by Waqas Qasim, Min Ho Lee, Byeong Eun Moon, Frank Gyan Okyere, Fawad Khan, Mohammad Nafees, Hyeon Tae Kim

“…Abstract Purpose This study aimed to achieve successful composting and aeration rate and to optimize the carbon:nitrogen (C:N) ratio to provide favourable conditions for the process. In the current experiment, investigation were made on variations in physico–chemical properties, i.e., temperature, ammonia and carbon dioxide emissions, pH, electrical conductivity (EC), organic matter (OM) and seed germination index (GI%) of composting chicken manure mixed with sawdust and wood shavings under different aeration rates in a closed reactor system. …”
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The lysosome-related characteristics affects the prognosis and tumor microenvironment of lung adenocarcinoma by Wuguang Chang, Wuyou Gao, Yawei Wu, Bin Luo, Lekai Zhong, Leqi Zhong, Wenqian Lin, Zhesheng Wen, Youfang Chen

“…Single cell sequencing (scRNA) was obtained from GEO to analyze the differences of lysosome risk signature at the single-cell level and the differences in the function and pathway. In vitro experiments have validated the function of CTSH in LUAD.ResultsThe risk signature contained seven key LAGs, and patients were categorized into high- and low-risk groups based on a specific calculation formula. …”
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Plant-microbe interactions influence plant performance via boosting beneficial root-endophytic bacteria by Sai Guo, Rubin Pan, Yan Zhang, Quanwei Gu, Qirong Shen, Jian Yang, Luqi Huang, Zongzhuan Shen, Rong Li

“…We further investigated the functional importance of these potentially beneficial bacteria on plant performance in subsequent greenhouse and field experiments. We found that root-endophytic Pseudomonas effectively increased medicinal plant seedling growth, crop yield, and the content of effective medicinal components. …”
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USP25 attenuates anti-GBM nephritis in mice by negative feedback regulation of Th17 cell differentiation by Ranran Xu, Fei Huang, Qingquan Liu, Yongman Lv, Liu Hu, Qian Zhang

“…Furthermore, changes in USP25 expression were monitored during Th17 differentiation, both in vivo and in vitro.Results USP25−/− mice with anti-GBM GN exhibited accelerated renal function deterioration, increased infiltration of Th1 and Th17 cells, and elevated RORγt transcription. In vitro experiments demonstrated that USP25−/− CD4+ T lymphocytes had a higher proportion for Th17 cell differentiation and exhibited higher RORγt levels upon stimulation. …”
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