Ex Vivo, In Vitro and In Vivo Bone Health Properties of Grana Padano Cheese by Cristina Martelli, Luisa Ottobrini, Anita Ferraretto, Paola Bendinelli, Stefano Cattaneo, Fabio Masotti, Milda Stuknytė, Margherita Dall’Asta, Angelo Del Sole, Ivano De Noni, Filippo Rossi

“…Overall, the ex vivo, in vitro and in vivo experiments highlighted the effectiveness of GP in improving Ca absorption, osteoblast proliferation and bone remodeling and volume.…”
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Th17/Treg balance is regulated during the suppression of experimental autoimmune encephalomyelitis treated by Astragalus polysaccharides via the microbiota-gut-brain axis by Jinyun Ma, Xiaojun Liu, Yan Zhao, Qijin Lu, Guiqing Ding, Yuanhua Wang, Xiaodong Cheng

“…It was further shown that APS inhibited gut inflammation and reduced Th17 function. The experiment with an antibiotic cocktail interfering with the gut microbiota proved that APS alleviated EAE by regulating the gut microbiota. …”
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Fibrin Glue Enhances Adipose-Derived Stromal Cell Cytokine Secretion and Survival Conferring Accelerated Diabetic Wound Healing by Ursula Hopfner, Matthias M. Aitzetmueller, Philipp Neßbach, Michael S. Hu, Hans-Guenther Machens, Zeshaan N. Maan, Dominik Duscher

“…To investigate the in vitro effect of fibrin glue, cultivation experiments were performed and key cytokines for regeneration were quantified. …”
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Experimental Study of Thoracoabdominal Injuries Suffered from Caudocephalad Impacts Using Pigs by Sishu Guan, Zhikang Liao, Hongyi Xiang, Xiyan Zhu, Zhong Wang, Hui Zhao, Peng Liu, Xinan Lai

“…To know the caudocephalad impact- (CCI-) induced injuries more clearly, 21 adult minipigs, randomly divided into three groups: control group (n=3), group I (n=9), and group II (n=9), were used to perform the CCI experiments on a modified deceleration sled. Configured impact velocity was 0 m/s in the control group, 8 m/s in group I, and 11 m/s in group II. …”
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Ddx21 mutant peptide is an effective neoantigen in prophylactic lung cancer vaccines and activates long-term anti-tumor immunity by Zhe Zhang, Yimeng Xia, Yimeng Xia, Zhihong Wang, Yaxing Sun, Dan Pu, Yijia He, Ruixian Liu, Yanru Zhang, Yan Liu, Junzhi Yu, Shiyang Ning, Baisui Feng, Yaohe Wang, Na Wang

“…MethodsTo investigate the fundamental reason for the difference between KPL 160302S and KPL 160424S vaccines, we employed bioinformatics methods and immune related experiments to explore the effects and mechanisms of the screened neoantigens. …”
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Fiber Bragg Grating Thermometry and Post-Treatment Ablation Size Analysis of Radiofrequency Thermal Ablation on Ex Vivo Liver, Kidney and Lung by Sanzhar Korganbayev, Leonardo Bianchi, Clara Girgi, Elva Vergantino, Domiziana Santucci, Eliodoro Faiella, Paola Saccomandi

“…Optical fibers embedding arrays of fiber Bragg grating (FBG) sensors, characterized by 0.1 °C accuracy and 1.2 mm spatial resolution, were employed for thermometry during the procedures. Experiments were conducted for all the organs in two different configurations: single-electrode (200 W for 12 min) and double-electrode (200 W for 9 min). …”
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Modeling automatic horizontation system of steep-sloping mobile machine with hydraulic sensor roll by V. I. Grishchenko, A. A. Tumakov, M. S. Poleshkin, M. S. Kilina, D. D. Dymochkin

“…A generalized mathematical model of the leveling system with a submodel of the control roll sensor has been developed. A computational experiment is performed in the Matlab Simulink software environment based on the modules of the Simscape subroutine using numerical methods, which allowed to identify processes under varying loads on the chassis of mobile machine. …”
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Development of a droplet digital PCR method for the detection of Ureaplasma urealyticum by Yong-Zhuo Zhou, Yun-Hu Zhao, Yan-Lan Chen, Hua Luo, Yu-lin Zhou, Bing Gu, Wei-Zhen Fang, Chao-Hui Duan, Xu-Guang Guo

“…With a high specificity, no positive microdrop were detected in eight negative control pathogens in this experiment. In addition, ddPCR detection of UU has good repeatability, and the calculated CV is 2.1 %. …”
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Polydopamine grafting polyether ether ketone to stabilize growth factor for efficient osteonecrosis repair by Yi Sun, Jingyun Liu, Kaijia Chen, Nannan Zhong, Chengpeng He, Xinming Luan, Xiaobei Zang, Jianbo Sun, Ning Cao, Wenbo Wang, Qiang Ren

“…A range of PEEK composite materials, including sulfonated polyether ether ketone (SPEEK), polydopamine-sulfonated polyether ether ketone (SPEEK-PDA), bone-forming peptide-poly-dopamine-sulfonated polyether ether ketone (SPEEK-PDA-BFP), and vascular endothelial growth factor-poly-dopamine-sulfonated polyether ether ketone (SPEEK-PDA-VEGF), were constructed by concentrated sulfuric acid sulfonation, polydopamine modification and grafting of bioactive factors. The experiments involved adult male New Zealand rabbits aged 24–28 weeks and weighing 2.6–4 kg. …”
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Transformation of NO in Combustion Gases by DC Corona by Oleksandr Molchanov, Kamil Krpec, Jiří Horák, Lenka Kuboňová, František Hopan, Jiří Ryšavý, Marcelina Bury

“…This study investigates the performance of DC corona discharge electrostatic precipitators (ESPs) for NO conversion to increase DeNO_x technologies’ efficiency for small-scale biomass combustion systems. Experiments were conducted using a 5 kW automatic wood pellet domestic heat source with combustion gas treated in a specially designed ESP operated in both positive and negative corona modes, resulting in a reduction in NO concentrations from 130 mg/m³ to 27/29 mg/m³ for positive/negative polarities (at 0 °C and 101.3 kPa). …”
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Effects of in-feed zeolite treatment on some biomarkers in dairy cows with subclinical mastitis by Dražen Đuričić, Silvijo Vince, Nino Maćešić, Damjan Gračner, Mislav Kovačić, Ivan Folnožić, Sonja Perkov, Marko Samardžija

“…A total of 69 Holstein dairy cows, aged between 2 and 7 years, kept on a farm in North-Western Croatia, were included in the experiment. The cows were randomly assigned into the following three groups: (1) clinoptilolite (CPL) group with subclinical mastitis (n = 20), treated with in-feed 50 g natural powdered clinoptilolite twice daily from the 270th day of pregnancy to the end of the study; (2) non-treated cows (n = 19) with subclinical mastitis (SCM); and (3) the control group (CON) of healthy cows (n = 30). …”
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H. Wendl Leaf Metabolites Potentiate the Radiosensitivity of Hepatocellular Carcinoma Through Ki67 and PARP Inhibition by Nabil M. Selim PhD, Seham S. El-Hawary PhD, Soheir M. El Zalabani PhD, Rehab Nabil Shamma PhD, Nariman El Sayed Mahdy PhD, Noheir H. Sherif PhD, Khayrya A. Youssif PhD, Usama Ramadan Abdelmohsen PhD, Mai H. Mekkawy PhD, Hanan A. Fahmy PhD

“…In the in vivo study, HCC was induced in male albino rats through administration of DEN in drinking water for 8 weeks, then treatment with NE (100 mg/kg) until the experiment’s ending. Rats were irradiated by a fractionated dose of 2Gy*4. …”
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A Laboratory Approach Considering Salinity to Investigate Archie’s Parameters in Deep Shale: A Case Study in Longmaxi Formation, Southern Sichuan by Yanran Li, Xiangui Liu, Changhong Cai, Zhiming Hu, Bo Wu, Ying Mu, Xianggang Duan, Qingxiu Zhang, Shuti Zeng, Jingshu Guo, Zhijin Pu

“…The mineral contents and petrophysical properties were firstly acquired through X-ray diffraction (XRD) and basic measurements. Rock-electric experiments under different salinity were conducted on deep shale samples taken from Longmaxi (LMX) Formation in Luzhou (LZ). …”
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Glutathione reductase plays a role in the metabolism of methylmercury degradation in Rhodotorula mucilaginosa by Yi Guo, Wenlong Deng, Qigui Mo, You Yu, Zhenwang Zhang, Mingjie Wei, Ruiling Tang, Surui Lu, Yanting Su

“…Structural domain analysis and molecular docking experiments suggest that the glutathione reductase of R. mucilaginosa has the potential to directly or indirectly participate in methylmercury degradation metabolism. …”
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Horses are worthy of care: Horse sector participants’ attitudes towards animal sentience, welfare, and well-being by Julie M Fiedler, Margaret L Ayre, Sarah Rosanowski, Josh D Slater

“…This requires providing positive experiences for animals which is critical to maintaining the social licence to operate. …”
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Identification of nuclear valosin-containing-protein-like as a target of anti-nuclear autoantibodies in systemic sclerosis by Zitao Zeng, Ramona Miske, Madeleine Scharf, Yvonne Denno, Anthonina Ott, Stefanie Brakopp, Bianca Teegen, Winfried Stöcker, Elise Siegert, Elise Siegert, Sandra Saschenbrecker, Christian Probst, Lars Komorowski

“…Immunoprecipitation with fixed HEp-2 cells followed by SDS-PAGE and MALDI-TOF mass spectrometry was used to identify the autoantigen, which was verified by competitive inhibition experiments, recombinant HEK293 cell-based IFA, and Western and line blots based on the recombinant antigen. …”
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Artificial intelligence assisted risk prediction in organ transplantation: a UK Live-Donor Kidney Transplant Outcome Prediction tool by Hatem Ali, Arun Shroff, Tibor Fülöp, Miklos Z. Molnar, Adnan Sharif, Bernard Burke, Sunil Shroff, David Briggs, Nithya Krishnan

“…Death-censored graft survival was the primary performance indicator. We experimented with four machine learning (ML) models assessed for calibration and discrimination [integrated Brier score (IBS) and Harrell’s concordance index]. …”
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Cooperative mechanisms of LexA and HtpG in the regulation of virulence gene expression in Pseudomonas plecoglossicida by Rongchao He, Yanfei Zuo, Qiu Li, Qingpi Yan, Lixing Huang

“…We employed an array of methods including molecular dynamics simulations, molecular docking, ChIP-seq, RNA-seq, mass spectrometry, gene mutagenesis, LacZ reporter assays, electrophoretic mobility shift assays, co-immunoprecipitation, and in vitro LexA degradation experiments. Our findings identified 36 downstream virulence genes regulated by LexA, define three critical LexA binding motifs, and provide an in-depth analysis of LexA's recognition and binding to promoters, thereby regulating virulence gene expression. …”
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Efficacy and safety of avacopan in antineutrophil cytoplasmic autoantibody-associated vasculitis: a retrospective cohort study in Japan by Genri Tagami, Makoto Yamaguchi, Hirokazu Sugiyama, Hiroshi Kinashi, Kentaro Imai, Keisuke Kamiya, Takayuki Katsuno, Takahiro Imaizumi, Shogo Banno, Yasuhiko Ito, Takuji Ishimoto

“…However, limited real-world data exist on the outcomes and experiences associated with avacopan use for AAV in Japan. …”
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Tannic acid-loaded inorganic-organic coating on magnesium bone nails for enhanced antibacterial, anti-inflammatory and corrosion resistance by Sirui Xu, Chuanyao Dong, Xiaolin Yu, Tianshu Lv, Dapeng Zeng, Ruiyan Li, Xingfu Bao, Kan Zhang

“…Moreover, the incorporation of tannic acid and chitosan imparts strong antibacterial and anti-inflammatory properties, achieving over 90% bacterial inhibition in vitro, mitigating infection risks, and enhancing osteogenic activity. In vivo experiments confirmed that the magnesium implant with the TA-CS/MAO coating exhibits superior antibacterial, anti-inflammatory, and osteoinductive properties. …”
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